FlyBase Allele Report: Dmel\SF2[GD12097]

General Information

Symbol

Dmel\SF2^GD12097

Species

D. melanogaster

Name

FlyBase ID

FBal0208442

Feature type

allele

Associated gene

Dmel\SF2

Associated Insertion(s)

Carried in Construct

P{GD12097}

Also Known As

UAS-SF2-RNAi, SRSF1-RNAi

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD12097}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD12097

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates amyotrophic lateral sclerosis

modeled by Zzzz\GGGGCC^36.UAS

(Hautbergue et al., 2017)

DOES NOT ameliorate amyotrophic lateral sclerosis

modeled by Zzzz\poly-GR^PO-36.UAS

(Hautbergue et al., 2017)

modeled by Zzzz\poly-PR^PO-36.UAS

(Hautbergue et al., 2017)

ameliorates frontotemporal dementia and/or amyotrophic lateral sclerosis 1

modeled by Zzzz\GGGGCC^36.UAS

(Castelli et al., 2021, Ortega et al., 2020)

ameliorates amyotrophic lateral sclerosis type 10

modeled by Hsap\TARDBP^UAS.TDPBR

(Pons et al., 2017)

ameliorates cancer

modeled by Pten^KK109278

(Parniewska and Stocker, 2020)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

ganglion mother cell | larval stage, with Scer\GAL4^insc-Mz1407

(Neumüller et al., 2011)

larval neuroblast | larval stage, with Scer\GAL4^insc-Mz1407

(Neumüller et al., 2011)

Detailed Description

Statement

Reference

Flies expressing SF2^GD12097 under the control of Scer\GAL4^GMR.PU have structurally normal eyes.

(Pons et al., 2017)

Expression under the control of Scer\GAL4¹⁴⁰⁷ results in loss of neuroblasts in the larval brain associated with increased size of the remaining neuroblasts.

Expression under the control of Scer\GAL4¹⁴⁰⁷ results in shorter neuroblast lineages (less daughter cells per neuroblast) in the larval brain compared to controls.

Expression under the control of Scer\GAL4¹⁴⁰⁷ may result in mild defects in the shape of ganglion mother cells in the larval brain (depending on the insertion line used).

Expression under the control of Scer\GAL4¹⁴⁰⁷ results in mild defects in the shape of neuroblasts in the larval brain.

(Neumüller et al., 2011)

Adults expressing SF2^GD12097 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) show significantly reduced avoidance of noxious temperature (46[o]C) compared to control flies.

(Neely et al., 2010)

Depending on the insertion line used, expression under the control of Scer\GAL4^Mef2.PR can result in viable flies or adult/pharate lethality.

(Schnorrer et al., 2010)

Expression under the control of Scer\GAL4^pnr-MD237 may result in lethality, depending on the insertion line used.

Expression under the control of Scer\GAL4^pnr-MD237 results in the absence of 20-30% of the Scer\GAL4^pnr-MD237-expressing area of the notum.

(Mummery-Widmer et al., 2009)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

12097

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

SF2^GD12097, Scer\GAL4^GMR.PU is a suppressor | partially of visible | adult stage phenotype of Scer\GAL4^GMR.PU, Zzzz\GGGGCC^30.UAS.EGFP

(Ortega et al., 2020)

SF2^GD12097, Scer\GAL4^GMR.PU is a suppressor of visible | adult stage phenotype of Scer\GAL4^GMR.PU, Zzzz\GGGGCC^36.UAS

(Ortega et al., 2020)

SF2^GD12097, Scer\GAL4^GMR.PF is a suppressor of visible | adult stage phenotype of Scer\GAL4^GMR.PF, Zzzz\GGGGCC^36.UAS

(Hautbergue et al., 2017)

SF2^GD12097, Scer\GAL4^da.G32 is a suppressor of abnormal locomotor behavior | adult stage phenotype of Scer\GAL4^da.G32, Zzzz\GGGGCC^36.UAS

(Hautbergue et al., 2017)

SF2^GD12097, Scer\GAL4^nSyb.PS is a suppressor | partially of abnormal locomotor behavior | third instar larval stage phenotype of Scer\GAL4^nSyb.PS, Zzzz\GGGGCC^36.UAS

(Hautbergue et al., 2017)

SF2^GD12097, Scer\GAL4^GMR.PU is a suppressor | partially of visible phenotype of Hsap\TARDBP^UAS.cPa, Scer\GAL4^GMR.PU

(Pons et al., 2017)

NOT Suppressor of

Statement

Reference

SF2^GD12097, Scer\GAL4^da.G32 is a non-suppressor of abnormal locomotor behavior | adult stage phenotype of Scer\GAL4^da.G32, Zzzz\poly-GR^PO-36.UAS

(Hautbergue et al., 2017)

SF2^GD12097, Scer\GAL4^da.G32 is a non-suppressor of abnormal locomotor behavior | adult stage phenotype of Scer\GAL4^da.G32, Zzzz\poly-PR^PO-36.UAS

(Hautbergue et al., 2017)

SF2^GD12097, Scer\GAL4^GMR.PF is a non-suppressor of visible | adult stage phenotype of Scer\GAL4^GMR.PF, Zzzz\poly-GR^PO-36.UAS

(Hautbergue et al., 2017)

SF2^GD12097, Scer\GAL4^GMR.PF is a non-suppressor of visible | adult stage phenotype of Scer\GAL4^GMR.PF, Zzzz\poly-PR^PO-36.UAS

(Hautbergue et al., 2017)

Phenotype Manifest In

Suppressor of

Statement

Reference

SF2^GD12097, Scer\GAL4^GMR.PU is a suppressor | partially of eye phenotype of Scer\GAL4^GMR.PU, Zzzz\GGGGCC^30.UAS.EGFP

(Ortega et al., 2020)

SF2^GD12097, Scer\GAL4^GMR.PU is a suppressor of eye phenotype of Scer\GAL4^GMR.PU, Zzzz\GGGGCC^36.UAS

(Ortega et al., 2020)

SF2^GD12097, Scer\GAL4^GMR.PF is a suppressor of eye | adult stage phenotype of Scer\GAL4^GMR.PF, Zzzz\GGGGCC^36.UAS

(Hautbergue et al., 2017)

SF2^GD12097, Scer\GAL4^GMR.PU is a suppressor | partially of eye phenotype of Hsap\TARDBP^UAS.cPa, Scer\GAL4^GMR.PU

(Pons et al., 2017)

NOT Suppressor of

Statement

Reference

SF2^GD12097, Scer\GAL4^GMR.PF is a non-suppressor of eye | adult stage phenotype of Scer\GAL4^GMR.PF, Zzzz\poly-GR^PO-36.UAS

(Hautbergue et al., 2017)

SF2^GD12097, Scer\GAL4^GMR.PF is a non-suppressor of eye | adult stage phenotype of Scer\GAL4^GMR.PF, Zzzz\poly-PR^PO-36.UAS

(Hautbergue et al., 2017)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

The co-expression of SF2^GD12097 fully suppresses the rough eye, fully suppresses the decreased adult locomotor activity, and partially suppresses the decreased larval locomotor activity induced by the expression of Zzzz\GGGGCC^36.Scer\UAS respectively under the control of Scer\GAL4^GMR.PF, Scer\GAL4^da.G32, and Scer\GAL4^nSyb.PS. SF2^GD12097 co-expression, however, is not able to suppress the severe rough eye phenotype or the decreased adult locomotor activity induced by the expression of either Zzzz\poly-GR^{PO-36.Scer\UAS} or Zzzz\poly-PR^{PO-36.Scer\UAS}under the control of Scer\GAL4^GMR.PF and Scer\GAL4^da.G32, respectively.

(Hautbergue et al., 2017)

SF2^GD12097 reduces the extent of the rough eye phenotype when co-expressed with a strongly expressing Hsap\TARDBP^UAS.cPa line under the control of Scer\GAL4^GMR.PU.

(Pons et al., 2017)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

VDRC

v27775

w¹¹¹⁸; P{GD12097}v27775/TM3

v27776

w¹¹¹⁸; P{GD12097}v27776/TM3