FlyBase Allele Report: Dmel\Pura[GD12260]

General Information

Symbol

Dmel\Pura^GD12260

Species

D. melanogaster

Name

FlyBase ID

FBal0203646

Feature type

allele

Associated gene

Dmel\Pura

Associated Insertion(s)

Carried in Construct

P{GD12260}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD12260}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD12260

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | adult stage, with Scer\GAL4^P0.5.Pdf

(Petsakou et al., 2015)

lethal, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010)

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

(Neely et al., 2010)

viable, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

viable, with Scer\GAL4^tin.CΔ4

(Neely et al., 2010)

Phenotype Manifest In

presynaptic active zone, with Scer\GAL4^P0.5.Pdf

(Petsakou et al., 2015)

s-LNv Pdf neuron, with Scer\GAL4^P0.5.Pdf

(Petsakou et al., 2015)

Detailed Description

Statement

Reference

Expression of Pura^GD12260 under the control of Scer\GAL4^P0.5.Pdf (limited to the adult stages using Scer\GAL80^ts.αTub84B) results in an increase in axonal volume and spread of s-LNv neuron projections compared to controls when expression is induced at ZT24 (dawn) and spread is measured 12 hours later. The spread of projections is unaffected when expressed is induced at ZT12 (dusk) and measured at dawn. Normal locomotor activity is seen when the flies are kept in complete darkness (DD conditions).

Expressing Pura^GD12260 for 14 hours under the control of Scer\GAL4^P0.5.Pdf (restricted to the adult stages using Scer\GAL80^ts.αTub84B) has no effect on the number of active zones in LNv projections at ZT2, but the normally low number of active zones seen at ZT14 is increased to the levels seen at ZT2 in controls.

(Petsakou et al., 2015)

Adults expressing Pura^GD12260 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

(Neely et al., 2010)

Expression under the control of Scer\GAL4^Mef2.PR results in adult/pharate lethality.

(Schnorrer et al., 2010)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

12260

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

Pura^GD12260, Scer\GAL4^P0.5.Pdf is a suppressor of abnormal circadian behavior phenotype of Rho1^UAS.cMa, Scer\GAL4^P0.5.Pdf

(Petsakou et al., 2015)

NOT Suppressor of

Statement

Reference

Pura^GD12260, Scer\GAL4^P0.5.Pdf is a non-suppressor of abnormal circadian behavior phenotype of Rho1^V14.UAS, Scer\GAL4^P0.5.Pdf

(Petsakou et al., 2015)

Pura^GD12260, Scer\GAL4^P0.5.Pdf is a non-suppressor of abnormal circadian behavior phenotype of Rac1^UAS.cLa, Scer\GAL4^P0.5.Pdf

(Petsakou et al., 2015)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

The behavioural arrhythmicity of flies overexpressing Rho1^Scer\UAS.cMa under the control of Scer\GAL4^P0.5.Pdf from birth is rescued is rescued upon co-expression of Pura^GD12260.

Expression of Pura^GD12260 does not suppress the increase in length of the behavioral rhythm period seen when Rac1^Scer\UAS.cLa is expressed under the control of Scer\GAL4^P0.5.Pdf.

Co-expression of Pura^GD12260 suppresses the reduction in axonal volume and spread of s-LNv neuron projections seen at ZT24 (dawn), following 12 hours of expression of Rho1^Scer\UAS.cMa under the control of Scer\GAL4^P0.5.Pdf (restricted to the adult stages using Scer\GAL80^ts.αTub84B).

Expression of Pura^GD12260 does not suppress the arrhythmicity seen in flies expressing Rho1^V14.Scer\UAS under the control of Scer\GAL4^P0.5.Pdf.

(Petsakou et al., 2015)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 1 )

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

12260

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

CG33275^GD12260

Pura^GD12260

Name Synonyms

Secondary FlyBase IDs

References (7)

Report Sections

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