FlyBase Allele Report: Dmel\Zip71B[GD2461]

General Information

Symbol

Dmel\Zip71B^GD2461

Species

D. melanogaster

Name

FlyBase ID

FBal0198867

Feature type

allele

Associated gene

Dmel\Zip71B

Associated Insertion(s)

Carried in Construct

P{GD2461}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD2461}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD2461

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates xanthinuria

modeled by ry^HMS02827

(Yin et al., 2017)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

chemical sensitive, with Scer\GAL4^NP1093

(Yin et al., 2017)

chemical sensitive, with Scer\GAL4^NP3084

(Yin et al., 2017)

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

(Neely et al., 2010)

viable, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010)

viable, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

viable, with Scer\GAL4^tin.CΔ4

(Neely et al., 2010)

visible | adult stage, with Scer\GAL4^GMR.long

(Dechen et al., 2015)

Phenotype Manifest In

eye, with Scer\GAL4^GMR.long

(Dechen et al., 2015)

Detailed Description

Statement

Reference

Under normal diet conditions, the expression of Zip71B^GD2461 under the control of either Scer\GAL4^{CG10962-NP1093} or Scer\GAL4^NP3084 does not have a significant effect on the eclosion rate, but these flies exhibit significantly decreased eclosion rates when fed with zinc (but not copper, iron, or EDTA) supplemented food, as compared to control flies. Under normal or zinc-supplemented diet, third instar larvae expressing Zip71B^GD2461 under the control of Scer\GAL4^{CG10962-NP1093} present a significant increase in the whole-body content of zinc, but not of Iron, Manganese or Copper, as compared to controls.

(Yin et al., 2017)

Ectopic expression of single copy of Zip71B^GD2461 under the control of Scer\GAL4^GMR.long does not produce any defects in the adult eye, when two copies of Zip71B^GD2461 as well as the Scer\GAL4^GMR.long driver are used the eye displays moderate loss of pigmentation, compared to the double driver control.

(Dechen et al., 2015)

Adults expressing Zip71B^GD2461 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

(Neely et al., 2010)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

2461

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT suppressed by

Statement

Reference

Scer\GAL4^NP1093, Zip71B^GD2461 has chemical sensitive phenotype, non-suppressible by Scer\GAL4^NP1093/ZnT63C^KK102261

(Yin et al., 2017)

Enhancer of

Statement

Reference

Scer\GAL4^GMR.long/Zip71B^GD2461 is an enhancer of visible | adult stage phenotype of Scer\GAL4^GMR.long, ZnT86D^UAS.EGFP

(Dechen et al., 2015)

Scer\GAL4^GMR.long/Zip71B^GD2461 is an enhancer of abnormal eye color phenotype of Scer\GAL4^GMR.long, ZnT86D^UAS.EGFP

(Dechen et al., 2015)

NOT Suppressor of

Statement

Reference

Scer\GAL4^GMR.long/Zip71B^GD2461 is a non-suppressor of visible | adult stage phenotype of Scer\GAL4^GMR.long, ZnT86D^UAS.cDa

(Dechen et al., 2015)

Scer\GAL4^GMR.long/Zip71B^GD2461 is a non-suppressor of abnormal eye color phenotype of Scer\GAL4^GMR.long, ZnT86D^UAS.cDa

(Dechen et al., 2015)

Scer\GAL4^GMR.long/Zip71B^GD2461 is a non-suppressor of visible | adult stage phenotype of Scer\GAL4^GMR.long, ZnT86D^UAS.EGFP

(Dechen et al., 2015)

Other

Statement

Reference

Hsap\SLC39A5^UAS.cYa, Scer\GAL4^NP1093, Zip71B^GD2461 has chemical sensitive phenotype

(Yin et al., 2017)

Phenotype Manifest In

Enhancer of

Statement

Reference

Scer\GAL4^GMR.long/Zip71B^GD2461 is an enhancer of eye phenotype of Scer\GAL4^GMR.long, ZnT86D^UAS.EGFP

(Dechen et al., 2015)

Suppressor of

Statement

Reference

Scer\GAL4^NP1093/Zip71B^GD2461 is a suppressor of embryonic/larval Malpighian tubule | third instar larval stage phenotype of Scer\GAL4^NP1093, ry^HMS02827

(Yin et al., 2017)

NOT Suppressor of

Statement

Reference

Scer\GAL4^GMR.long/Zip71B^GD2461 is a non-suppressor of eye phenotype of Scer\GAL4^GMR.long, ZnT86D^UAS.cDa

(Dechen et al., 2015)

Scer\GAL4^GMR.long/Zip71B^GD2461 is a non-suppressor of eye phenotype of Scer\GAL4^GMR.long, ZnT86D^UAS.EGFP

(Dechen et al., 2015)

Additional Comments

Genetic Interactions

Statement

Reference

The co-expression of ZnT63C^KK102261 does not suppress the decreased eclosion rate exhibited by animals expressing Zip71B^GD2461 under the control of Scer\GAL4^{CG10962-NP1093} reared in zinc-supplemented food; under normal diet conditions, the co-expression of Zip71B^GD2461 and ZnT63C^KK102261 under the control of Scer\GAL4^{CG10962-NP1093} has no significant effect on the eclosion rate, as compared to animals only expressing either Zip71B^GD2461 or ZnT63C^KK102261 under the control of Scer\GAL4^{CG10962-NP1093}.

The formation of Malpighian tubule stones in third instar larvae induced by the expression of ry^HMS02827 under the control of Scer\GAL4^{CG10962-NP1093} is suppressed by the co-expression of Zip71B^GD2461.

(Yin et al., 2017)

The morphological and pigmentation defects in the adult eye seen upon expression of ZnT86D^Scer\UAS.cDa under the control of Scer\GAL4^GMR.long do not change upon co-expression of Zip71B^GD2461 RNAi.

The adult eye phenotype seen upon expression of ZnT86D^{Scer\UAS.T:Avic\GFP-EGFP} under the control of Scer\GAL4^GMR.long can be partially modified by co-expression of Zip71B^GD2461 RNAi: the morphological defects are unchanged the pigmentation loss is mildly worsened.

(Dechen et al., 2015)

Xenogenetic Interactions

Statement

Reference

The co-expression of Hsap\SLC39A5^Scer\UAS.cYa, but not of Hsap\SLC39A4^Scer\UAS.cYa, significantly increases the eclosion rate of animals expressing Zip71B^GD2461 under the control of Scer\GAL4^{CG10962-NP1093} when reared under zinc-supplemented diet, but not when reared under normal diet.

(Yin et al., 2017)

Complementation and Rescue Data

Rescues

Scer\GAL4^GMR.long/Zip71B^GD2461 rescues Zip71B^UAS.Tag:FLAG

(Dechen et al., 2015)

Comments

Expression of Zip71B^GD2461 under the control of Scer\GAL4^GMR.long rescues the flat eye shape and loss of pigmentation induced by Zip71B^{Scer\UAS.T:Zzzz\FLAG} expression.

(Dechen et al., 2015)

Images (0)

Mutant

Wild-type

Stocks (1)

VDRC

v44538

w¹¹¹⁸; P{GD2461}v44538

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 1 )

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

2461

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

CG10006^GD2461

Zip71B-RNAi 1

(Yin et al., 2017)

Zip71B^GD2461

dZip71B^RNAi

(Dechen et al., 2015)

Name Synonyms

Secondary FlyBase IDs

References (8)

Report Sections

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