Mobilisation of P{lacW}gloS011046 produces an approximately 1.1kb deletion beginning 576bp upstream and ending 512bp downstream of the translation start codon. The deletion removes the glo transcription start site and 5' flanking region, the first and second exons, and part of the second intron for all predicted splice forms.
A ~1.1kb deletion resulting from the imprecise excision of P{lacW}gloS011046 that removes sequences from 576bp upstream to 512bp downstream of the translation start codon.
chromatin & nurse cell | germ-line clone
Approximately 30% of eggs laid by females with glo162x germ-line clones have abnormal dorsal appendages, ranging from short, wide appendages to fused appendages that extend laterally around the anterior of the egg. Late oocytes show a higher frequency (55%) of dorsal appendage defects, suggesting that some of these oocytes never mature as eggs. In glo162x ovaries, some egg chambers fail to undergo nurse cell dumping. In nurse cells from glo162x ovaries, chromosomes fail to disperse during mid-oogenesis and maintain a polytene morphology.
Homozygotes survive until the third instar larval stage. The class IV da neurons of the mutant larvae show a significant decrease in the number of higher order dendritic branches, as reflected by a decreased number of terminal dendritic processes.
Homozygous class IV da neuron clones show a reduction in the density of terminal dendritic branches in mosaic third instar larvae.
Over 60% of eggs produced by females with glo162x mutant ovaries (somatic clones) fail to develop beyond blastoderm stages. Between 10% and 20% of these display gross morphological defects, including abnormal dorsal appendages, collapsed appearance, or shortened egg length and are frequently unfertilised.
Approximately 25% of eggs produced by females with glo162x mutant ovaries (somatic clones) develop to hatching, while a small and variable fraction (5-10%) show defects in anterior-posterior patterning, including loss of anterior structures, loss of abdominal segments, and bicaudal phenotypes.
glo162x has visible | germline clone | egg stage phenotype, suppressible by otu104
glo162x has dorsal appendage | germline clone phenotype, suppressible by otu104
glo162x has nurse cell | germline clone phenotype, suppressible | partially by otu104
