FlyBase Allele Report: Dmel\dco[UAS.cKa]

General Information

Symbol

Dmel\dco^UAS.cKa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Klein

FlyBase ID

FBal0194129

Feature type

allele

Associated gene

Dmel\dco

Associated Insertion(s)

Carried in Construct

P{UAS-dco.K}

Also Known As

UAS-dco

Key Links

Transgenic product class

wild_type

(Klein et al., 2006)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Klein et al., 2006)

Mutagen

in vitro construct

(Klein et al., 2006)

Progenitor genotype

Carried in construct

P{UAS-dco.K}

Cytology

Description

UAS regulatory sequences drive expression of dco.

(Klein et al., 2006)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

dco

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates amyotrophic lateral sclerosis type 6

modeled by Hsap\FUS^P525L.UAS

(Kishino et al., 2022)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal cell polarity, with Scer\GAL4^dpp.blk1

(Klein et al., 2006)

abnormal cell polarity, with Scer\GAL4^hs.2sev

(Klein et al., 2006)

Phenotype Manifest In

eye photoreceptor cell, with Scer\GAL4^hs.2sev

(Klein et al., 2006)

ommatidium, with Scer\GAL4^hs.2sev

(Klein et al., 2006)

wing hair, with Scer\GAL4^dpp.blk1

(Klein et al., 2006)

wing margin bristle | increased number, with Scer\GAL4^dpp.blk1

(Klein et al., 2006)

Detailed Description

Statement

Reference

Expression of dco^Scer\UAS.cKa under the control of Scer\GAL4^hs.2sev, results in planar polarity defects in the eye. A small percentage of ommatidia in eyes of this genotype show a change in photoreceptor number.

Expression of dco^Scer\UAS.cKa under the control of Scer\GAL4^dpp.blk1 in the wing causes a mild planar cell polarity defect visible as a characteristic hair swirl near the intersection of the dpp expression stripe and the wing margin. These wings also show a small increase in the number of margin bristles.

(Klein et al., 2006)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Scer\GAL4^dpp.blk1, dco^UAS.cKa has abnormal cell polarity phenotype, enhanceable by Scer\GAL4^dpp.blk1/fz^{1-1-1.UAS.Tag:MYC}

(Klein et al., 2006)

Suppressed by

Statement

Reference

Scer\GAL4^hs.2sev, dco^UAS.cKa has abnormal cell polarity phenotype, suppressible by dsh³/dsh[+]

(Klein et al., 2006)

Enhancer of

Statement

Reference

dco^UAS.cKa/Scer\GAL4^P2.4.Pdf is an enhancer of abnormal locomotor rhythm phenotype of Scer\GAL4^P2.4.Pdf, lark^UAS.Tag:HA

(Huang et al., 2014)

Scer\GAL4^bbg-C96/dco^UAS.cKa is an enhancer of visible phenotype of Scer\GAL4^bbg-C96, dom^RNAi.Sym.UAS

(Kwon et al., 2013)

Scer\GAL4^dpp.blk1/dco^UAS.cKa is an enhancer of abnormal cell polarity phenotype of fz^{1-1-1.UAS.Tag:MYC}

(Klein et al., 2006)

Suppressor of

Statement

Reference

dco^UAS.cKa/Scer\GAL4^P2.4.Pdf is a suppressor | partially of abnormal locomotor rhythm phenotype of Scer\GAL4^P2.4.Pdf, lark¹, lark^RNAi.UAS.cSb

(Huang et al., 2014)

Other

Statement

Reference

Scer\GAL4^dpp.blk1, dco^UAS.cKa, fz2^{2-2-2.UAS.Tag:MYC} has lethal | heat sensitive phenotype

(Klein et al., 2006)

Phenotype Manifest In

Enhanced by

Statement

Reference

Scer\GAL4^dpp.blk1, dco^UAS.cKa has wing margin bristle | increased number phenotype, enhanceable by Scer\GAL4^dpp.blk1/fz^{1-1-1.UAS.Tag:MYC}

(Klein et al., 2006)

Suppressed by

Statement

Reference

Scer\GAL4^hs.2sev, dco^UAS.cKa has ommatidium phenotype, suppressible by dsh³/dsh[+]

(Klein et al., 2006)

Scer\GAL4^hs.2sev, dco^UAS.cKa has eye photoreceptor cell phenotype, suppressible by dsh³/dsh[+]

(Klein et al., 2006)

Enhancer of

Statement

Reference

Scer\GAL4^bbg-C96/dco^UAS.cKa is an enhancer of wing margin phenotype of Scer\GAL4^bbg-C96, dom^RNAi.Sym.UAS

(Kwon et al., 2013)

Scer\GAL4^dpp.blk1/dco^UAS.cKa is an enhancer of wing hair phenotype of fz^{1-1-1.UAS.Tag:MYC}

(Klein et al., 2006)

Scer\GAL4^dpp.blk1/dco^UAS.cKa is an enhancer of wing margin bristle | ectopic phenotype of Scer\GAL4^dpp.blk1, fz2^{2-2-2.UAS.Tag:MYC}

(Klein et al., 2006)

Additional Comments

Genetic Interactions

Statement

Reference

Co-expression of dco^Scer\UAS.cKa significantly suppresses circadian rhythm defects in flies with lark^{dsRNA.Scer\UAS.cSb} driven by Scer\GAL4^P2.4.Pdf in a lark¹/+ background.

Conditional adult-specific co-expression (using TubGAL80ts) of dco^Scer\UAS.cKa (but not dco^{D132N.Scer\UAS}) results in arrhythmicity in flies with expression of lark^{Scer\UAS.T:Ivir\HA1} driven by Scer\GAL4^P2.4.Pdf.

(Huang et al., 2014)

The wing nicking phenotype caused by expression of dom^{dsRNA.Sym.Scer\UAS} under the control of Scer\GAL4^bbg-C96 is enhanced if the flies are also co-expressing dco^Scer\UAS.cKa.

(Kwon et al., 2013)

Coexpression of fz^{1-1-1.Scer\UAS.T:Hsap\MYC} and dco^Scer\UAS.cKa, under the control of Scer\GAL4^dpp.blk1, results in an enhancement of both the planar cell polarity defects seen when only fz^{1-1-1.Scer\UAS.T:Hsap\MYC} is expressed and an increase number of ectopic wing margin bristles produced relative to when only dco^Scer\UAS.cKa is expressed.

Co-expression of fz2^{2-2-2.Scer\UAS.T:Hsap\MYC} and dco^Scer\UAS.cKa, under the control of Scer\GAL4^dpp.blk1, is lethal when animals are raised at 25^oC. However, these animals are viable at 18^oC but show an increase in the number of ectopic wing margin bristles relative to expression of fz2^{2-2-2.Scer\UAS.T:Hsap\MYC} alone.

Expression of dco^Scer\UAS.cKa under the control of Scer\GAL4^hs.2sev in a dsh³/+ background completely suppresses the planar cell polarity and photoreceptor number phenotypes seen when dco^Scer\UAS.cKa is expressed in a wild-type background.

(Klein et al., 2006)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

26274

w^*; P{UAS-dco.K}4

26269

w^*; P{UAS-dco.K}1/CyO

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

dco^Scer\UAS.cKa

dco^UAS.cKa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Klein

Secondary FlyBase IDs

References (6)

Report Sections

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