FlyBase Allele Report: Dmel\fz2[2-2-2.UAS.Tag:MYC]

General Information

Symbol

Dmel\fz2^{2-2-2.UAS.Tag:MYC}

Species

D. melanogaster

Name

FlyBase ID

FBal0118225

Feature type

allele

Associated gene

Dmel\fz2

Associated Insertion(s)

Carried in Construct

P{UAS-fz2-2}

Also Known As

UAS-fz2

Key Links

Transgenic product class

cDNA

(Boutros et al., 2000)

wild_type

(Boutros et al., 2000)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Boutros et al., 2000)

wild_type

(Boutros et al., 2000)

Mutagen

in vitro construct

(Boutros et al., 2000)

Progenitor genotype

Carried in construct

P{UAS-fz2-2}

Cytology

Description

UAS regulatory sequences drive expression of a fz2 cDNA that has been tagged with Tag:MYC in the non-conserved region in between the CRD and the transmembrane domains.

(Boutros et al., 2000)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

fz2

Tagged with

Tag:MYC

epitope tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal, with Scer\GAL4^en.PU

(Serysheva et al., 2013)

Phenotype Manifest In

wing margin bristle | ectopic, with Scer\GAL4^dpp.blk1

(Serysheva et al., 2013, Klein et al., 2006)

Detailed Description

Statement

Reference

Scer\GAL4^dpp.blk1-mediated expression of fz2^{2-2-2.Scer\UAS.T:Hsap\MYC} induces ectopic wing margin bristles.

(Serysheva et al., 2013)

Expression of fz2^{2-2-2.Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^dpp.blk1 at 18^oC induces a small number of ectopic wing margin bristles, while expression at 25^oC induces many ectopic bristles.

(Klein et al., 2006)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Scer\GAL4^en.PU, fz2^{2-2-2.UAS.Tag:MYC} has lethal phenotype, suppressible by Scer\GAL4^en.PU/Wnk^KK102654

(Serysheva et al., 2013)

Other

Statement

Reference

Scer\GAL4^dpp.blk1, dco^UAS.cKa, fz2^{2-2-2.UAS.Tag:MYC} has lethal | heat sensitive phenotype

(Klein et al., 2006)

Phenotype Manifest In

Enhanced by

Statement

Reference

Scer\GAL4^dpp.blk1, fz2^{2-2-2.UAS.Tag:MYC} has wing margin bristle | ectopic phenotype, enhanceable by Wnk^UAS.Tag:HA/Scer\GAL4^dpp.blk1

(Serysheva et al., 2013)

Scer\GAL4^dpp.blk1, fz2^{2-2-2.UAS.Tag:MYC} has wing margin bristle | ectopic phenotype, enhanceable by Scer\GAL4^dpp.blk1/dco^UAS.cKa

(Klein et al., 2006)

NOT Enhanced by

Statement

Reference

Scer\GAL4^dpp.blk1, fz2^{2-2-2.UAS.Tag:MYC} has wing margin bristle | ectopic phenotype, non-enhanceable by Scer\GAL4^dpp.blk1/fray^KK100424

(Serysheva et al., 2013)

Scer\GAL4^dpp.blk1, fz2^{2-2-2.UAS.Tag:MYC} has wing margin bristle | ectopic phenotype, non-enhanceable by Df(3R)ED2/+

(Serysheva et al., 2013)

Scer\GAL4^dpp.blk1, fz2^{2-2-2.UAS.Tag:MYC} has wing margin bristle | ectopic phenotype, non-enhanceable by Scer\GAL4^dpp.blk1/dco^D132N.UAS

(Klein et al., 2006)

Suppressed by

Statement

Reference

Scer\GAL4^dpp.blk1, fz2^{2-2-2.UAS.Tag:MYC} has wing margin bristle | ectopic phenotype, suppressible by Scer\GAL4^dpp.blk1/Wnk^KK102654

(Serysheva et al., 2013)

NOT suppressed by

Statement

Reference

Scer\GAL4^dpp.blk1, fz2^{2-2-2.UAS.Tag:MYC} has wing margin bristle | ectopic phenotype, non-suppressible by Scer\GAL4^dpp.blk1/fray^KK100424

(Serysheva et al., 2013)

Scer\GAL4^dpp.blk1, fz2^{2-2-2.UAS.Tag:MYC} has wing margin bristle | ectopic phenotype, non-suppressible by Df(3R)ED2/+

(Serysheva et al., 2013)

Enhancer of

Statement

Reference

Scer\GAL4^C587/fz2^{2-2-2.UAS.Tag:MYC} is an enhancer of spectrosome | oogenesis | increased number phenotype of Scer\GAL4^C587, dlp^UAS.cBa

(Waghmare et al., 2020)

Scer\GAL4^C587/fz2^{2-2-2.UAS.Tag:MYC} is an enhancer of female germline stem cell | decreased number phenotype of Scer\GAL4^C587, dlp^UAS.cBa

(Waghmare et al., 2020)

NOT Enhancer of

Statement

Reference

Scer\GAL4^C587/fz2^{2-2-2.UAS.Tag:MYC} is a non-enhancer of escort cell | decreased number phenotype of Scer\GAL4^C587, dlp^UAS.cBa

(Waghmare et al., 2020)

NOT Suppressor of

Statement

Reference

Scer\GAL4^C587/fz2^{2-2-2.UAS.Tag:MYC} is a non-suppressor of escort cell | decreased number phenotype of Scer\GAL4^C587, dlp^UAS.cBa

(Waghmare et al., 2020)

Other

Statement

Reference

Scer\GAL4^dpp.blk1, Wnk^KK102654, fz2^{2-2-2.UAS.Tag:MYC} has wing margin phenotype

(Serysheva et al., 2013)

Scer\GAL4^en.PU, Wnk^KK102654, fz2^{2-2-2.UAS.Tag:MYC} has wing margin phenotype

(Serysheva et al., 2013)

Scer\GAL4^en.PU, Wnk^KK102654, fz2^{2-2-2.UAS.Tag:MYC} has wing margin bristle phenotype

(Serysheva et al., 2013)

Additional Comments

Genetic Interactions

Statement

Reference

Co-expression of Wnk^KK102654 suppresses the ectopic margin bristles seen in Scer\GAL4^dpp.blk1 fz2^{2-2-2.Scer\UAS.T:Hsap\MYC} wings - such wings also show a loss of wing margin.

Co-expression of Wnk^KK102654 suppresses the lethality of Scer\GAL4^en.PU fz2^{2-2-2.Scer\UAS.T:Hsap\MYC}. Wings of surviving animals show partial loss of wing margin and lack margin bristles.

Co-expression of Wnk^{Scer\UAS.T:Ivir\HA1} increases the number of ectopic margin bristles seen in Scer\GAL4^dpp.blk1 fz2^{2-2-2.Scer\UAS.T:Hsap\MYC} wings.

Co-expression of fray^KK100424 or Df(3R)ED2/+ has no effect on the ectopic margin bristles seen in Scer\GAL4^dpp.blk1 fz2^{2-2-2.Scer\UAS.T:Hsap\MYC} wings.

(Serysheva et al., 2013)

Co-expression of fz2^{2-2-2.Scer\UAS.T:Hsap\MYC} and dco^Scer\UAS.cKa, under the control of Scer\GAL4^dpp.blk1, is lethal when animals are raised at 25^oC. However, these animals are viable at 18^oC but show an increase in the number of ectopic wing margin bristles relative to expression of fz2^{2-2-2.Scer\UAS.T:Hsap\MYC} alone.

Coexpression of fz2^{2-2-2.Scer\UAS.T:Hsap\MYC} and dco^{D132N.Scer\UAS}, under the control of Scer\GAL4^dpp.blk1, at 18^oC does not lead to an enhancement in the number of ectopic wing margin bristles compared to expression of fz2^{2-2-2.Scer\UAS.T:Hsap\MYC} alone. Coexpression of fz2^{2-2-2.Scer\UAS.T:Hsap\MYC} and dco^{D132N.Scer\UAS} at 25^oC is not lethal.

(Klein et al., 2006)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

41793

w^*; P{UAS-fz2-2}65/CyO

41794

w^*; P{UAS-fz2-2}16/TM6B, Tb⁺

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

fz2^{2-2-2.Scer\UAS.T:Hsap\MYC}

fz2^{2-2-2.UAS.Tag:MYC}

fz2^{2-2.Scer\UAS.T:Hsap\MYC}

Name Synonyms

Secondary FlyBase IDs

References (7)

Report Sections

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