FlyBase Allele Report: Dmel\hyx[2]

General Information

Symbol

Dmel\hyx²

Species

D. melanogaster

Name

FlyBase ID

FBal0193884

Feature type

allele

Associated gene

Dmel\hyx

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

Mutagen

ethyl methanesulfonate

(Mosimann et al., 2006)

Progenitor genotype

hyx^EP9

(Mosimann et al., 2009, Mosimann et al., 2006)

Cytology

Description

Amino acid replacement: Q461term.

(Mosimann et al., 2006)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

3R:9,041,576..9,041,576 [+]

Nucleotide change:

C9041576T

Amino acid change:

Q461term | hyx-PA; Q461term | hyx-PB

Reported amino acid change:

Q461term

Comment:

Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

(Mosimann et al., 2006)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

FlyBase_internaltransgene_features:cell lethal:cell lethal | somatic clone

(Mosimann et al., 2006)

abnormal cell number | embryonic stage (with hyx^EY06898)

(Bahrampour and Thor, 2016)

abnormal neuroanatomy | embryonic stage (with hyx^EY06898)

(Bahrampour and Thor, 2016)

lethal - all die during embryonic stage

(Mosimann et al., 2006)

some die during embryonic stage

(Mosimann et al., 2006)

Phenotype Manifest In

apterous-expressing neuron of the lateral cluster of the thorax | embryonic stage (with hyx^EY06898)

(Bahrampour and Thor, 2016)

eye | somatic clone

(Mosimann et al., 2006)

leg | somatic clone

(Mosimann et al., 2006)

macrochaeta | somatic clone

(Mosimann et al., 2006)

wing margin | somatic clone

(Mosimann et al., 2006)

wing vein | somatic clone

(Mosimann et al., 2006)

Detailed Description

Statement

Reference

The number of cells expressing particular neuropeptide markers in the ap cell cluster of the NB5-6T lineage is changed relative to controls (number of eya expressing cells is increased, no. of FMRFa or Nplp1 positive cells is decreased) in hyx²/hyx^EY06898 transheterozygous embryos.

(Bahrampour and Thor, 2016)

Flies with hyx² mutant clones in their adult tissue, generated using the Minute technique, show notches in the wing, ectopic wing vein material, deformed legs and deformed eyes. Flies with hyx² clones in the notum lack thoracic dorsal macrochaetae.

(Mosimann et al., 2006)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

hyx² has lethal | embryonic stage phenotype, suppressible by Hsap\CDC73^{αTub84B.Tag:HA}

(Mosimann et al., 2006)

NOT suppressed by

Statement

Reference

hyx² has lethal | embryonic stage phenotype, non-suppressible by Scer\CDC73^{αTub84B.Tag:HA}

(Mosimann et al., 2006)

NOT Enhancer of

Statement

Reference

Scer\GAL4^salm.E/hyx² is a non-enhancer of visible phenotype of Scer\GAL4^salm.E, ptc^UAS.Tag:MYC

(Mosimann et al., 2009)

NOT Suppressor of

Statement

Reference

Scer\GAL4^salm.E/hyx² is a non-suppressor of visible phenotype of Scer\GAL4^salm.E, ptc^UAS.Tag:MYC

(Mosimann et al., 2009)

Phenotype Manifest In

NOT Suppressor of

Statement

Reference

Scer\GAL4^salm.E/hyx² is a non-suppressor of wing blade phenotype of Scer\GAL4^salm.E, ptc^UAS.Tag:MYC

(Mosimann et al., 2009)

hyx² is a non-suppressor of wing margin phenotype of Scer\GAL4^salm.E, lgs^17E.UAS

(Mosimann et al., 2006)

Additional Comments

Genetic Interactions

Statement

Reference

The presence of a hyx² mutant background has no effect on the L2-L5 intervein growth and patterning defects resulting from the expression of ptc^{Scer\UAS.T:Hsap\MYC} driven by Scer\GAL4^salm.E.

(Mosimann et al., 2009)

hyx² fails to suppress the notched wing of Scer\GAL4^salm.E>lgs^17E.Scer\UAS flies.

(Mosimann et al., 2006)

Xenogenetic Interactions

Statement

Reference

Expression of the Hsap\CDC73^{αTub84B.T:Ivir\HA1} transgene carrying the human homologue of hyx rescues the lethality of hyx² and hyx¹/hyx² flies. Rescued flies how no obvious phenotypic defects. In contrast, a transgene carrying the yeast hyx homologue (Scer\CDC73^{αTub84B.T:Ivir\HA1}) fails to rescue these mutants.

(Mosimann et al., 2006)

Complementation and Rescue Data

Fails to complement

hyx¹

(Mosimann et al., 2006)

Rescued by

hyx¹/hyx² is rescued by hyx^{WT.αTub84B.Tag:HA}

(Goetze et al., 2009)

hyx¹/hyx² is rescued by hyx^{A2P.αTub84B.Tag:HA}

(Goetze et al., 2009)

Partially rescued by

hyx¹/hyx² is partially rescued by hyx^{A2P.αTub84B.Tag:HA}

(Goetze et al., 2009)

Comments

hyx^{WT.αTub84B.T:Ivir\HA1} fully rescues hyx¹/hyx² to adulthood at 18[o]C as well as at 25[o]C and 29[o]C, without obvious phenotypic defects and at the expected Mendelian ratios.

hyx^{A2P.αTub84B.T:Ivir\HA1} fully rescues hyx¹/hyx² to adulthood at 18[o]C as well as at 25[o]C, without obvious phenotypic defects and at the expected Mendelian ratios. However, at 29[o]C, hyx^{A2P.αTub84B.T:Ivir\HA1} shows a 50% reduction in rescue capability compared to controls.

(Goetze et al., 2009)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

EP-hyx²

(Mosimann et al., 2009)

hyx²

(Bahrampour and Thor, 2016, Goetze et al., 2009, Mosimann et al., 2009, Mosimann et al., 2006)

Name Synonyms

Secondary FlyBase IDs

References (4)

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