FlyBase Allele Report: Dmel\PDZ-GEF[EP388]

General Information

Symbol

Dmel\PDZ-GEF^EP388

Species

D. melanogaster

Name

FlyBase ID

FBal0193629

Feature type

allele

Associated gene

Dmel\PDZ-GEF

Associated Insertion(s)

P{EP}PDZ-GEF^EP388

Carried in Construct

Also Known As

Gef26², EP(2)0388, EP(2)388

Key Links

Genomic Maps

JBrowse

Allele class

hypomorphic allele - genetic evidence

Mutagen

Nature of the Allele

Allele class

hypomorphic allele - genetic evidence

(Wang et al., 2006)

Mutagen

Progenitor genotype

Associated Insertion(s)

P{EP}PDZ-GEF^EP388

Cytology

Description

Insertion of a P{EP} element at position +39 of exon 0.

(Huelsmann et al., 2006)

Insertion in the 5' upstream region of the Gef26 gene.

(Lee et al., 2002)

Allele components

Component

Use(s)

Inserted element

P{EP}

misexpression element

Regulatory region(s)

UAS

binary expression system - regulatory region

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

2L:6,323,797..6,323,797 [+]2L:6,323,660..6,323,660

(Huelsmann et al., 2006, Dupuy et al., 2005, Lee et al., 2002)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal cell shape, with Scer\GAL4^srp

(Huelsmann et al., 2006)

partially lethal

(Singh et al., 2006)

partially lethal (with PDZ-GEF³)

(Singh et al., 2006)

partially lethal (with PDZ-GEF^k13720)

(Singh et al., 2006)

viable

(Huelsmann et al., 2006)

visible

(Singh et al., 2006)

visible, with Scer\GAL4^GMR.PF

(Lee et al., 2002)

visible, with Scer\GAL4^hs.2sev

(Lee et al., 2002)

visible (with PDZ-GEF³)

(Singh et al., 2006)

visible (with PDZ-GEF^k13720)

(Singh et al., 2006, Lee et al., 2002)

Phenotype Manifest In

embryonic/larval plasmatocyte, with Scer\GAL4^srp

(Huelsmann et al., 2006)

eye

(Singh et al., 2006)

eye, with Scer\GAL4^GMR.PF

(Lee et al., 2002)

eye, with Scer\GAL4^hs.2sev

(Lee et al., 2002)

eye (with PDZ-GEF³)

(Singh et al., 2006)

eye (with PDZ-GEF^k13720)

(Singh et al., 2006, Lee et al., 2002)

eye photoreceptor cell (with PDZ-GEF^k13720)

(Lee et al., 2002)

eye photoreceptor cell | ectopic, with Scer\GAL4^GMR.PF

(Lee et al., 2002)

larval ventral nerve cord, with Scer\GAL4^srp

(Huelsmann et al., 2006)

ommatidium, with Scer\GAL4^GMR.PF

(Lee et al., 2002)

wing

(Singh et al., 2006)

wing (with PDZ-GEF³)

(Singh et al., 2006)

wing (with PDZ-GEF^k13720)

(Singh et al., 2006)

Detailed Description

Statement

Reference

Expression of one copy of Gef26^EP388 in macrophages, driven by Scer\GAL4^srp, does not affect macrophage migration but causes the formation of abnormally long protrusions from the macrophages. The protrusions from different macrophages contact one another, resulting in the formation of a network that either spans the nervous system in a dorsoventral directions or that is seen below the epidermis at lateral positions. In addition, cell shape is changed dramatically. Expression of two copies of Scer\GAL4^srp (also under the control of Scer\GAL4^srp) causes the migration of the macrophages to slow down and results in a gap at the ventral nerve cord after stage 13, which is closed later on in embryogenesis.

Macrophages that express Gef26^EP388, under the control of Scer\GAL4^srp, leave long stable protrusions behind during their migration. These extensions appear not to be formed as independent entities, but originate either from retracted lamellopodia or more frequently from the tail of migrating cells. At later stages, the long extensions are maintained in parallel to lamellopodia and do not disturb the normal local mobility of the cells.

(Huelsmann et al., 2006)

Eye and wing phenotypes (small rough eyes, abnormal wings) and some degree of lethality are seen in various PDZ-GEF allelic combinations, with their relative strength in the following order: PDZ-GEF⁴/PDZ-GEF⁶ = PDZ-GEF⁴/PDZ-GEF⁵ > PDZ-GEF³/PDZ-GEF⁴ > PDZ-GEF^k13720/PDZ-GEF³ = PDZ-GEF^k13720/PDZ-GEF⁴ > PDZ-GEF^EP388/PDZ-GEF³ > PDZ-GEF^k13720/PDZ-GEF^k13720 > PDZ-GEF^k13720/PDZ-GEF^EP388 > PDZ-GEF^EP388/PDZ-GEF^EP388.

Many mutant females have three spermathecae instead of two, with different allelic combinations having different percentages of females with three spermathecae: PDZ-GEF³/PDZ-GEF³ 22%, PDZ-GEF³/PDZ-GEF⁴ 30%, PDZ-GEF⁴/PDZ-GEF⁵ 59%, PDZ-GEF⁴/PDZ-GEF⁶ 62%, PDZ-GEF^k13720/PDZ-GEF⁴ % (all PDZ-GEF^k13720/PDZ-GEF⁴, PDZ-GEF^k13720/PDZ-GEF³ or PDZ-GEF^EP388/PDZ-GEF³ females have two spermathecae).

(Singh et al., 2006)

Gef26^EP388 flies have no visible mutant phenotype.

Gef26^k13720/Gef26^EP388 flies have a slight rough eye phenotype, with some missing photoreceptor cells.

Expression of Gef26^EP388 under the control of Scer\GAL4^GMR.PF results in a rough eye phenotype, without a reduction in total size of the eye compared to controls. Photoreceptor cell numbers are increased in some ommatidia and some ommatidial fusion is seen.

(Lee et al., 2002)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

PDZ-GEF^EP388, Scer\GAL4^GMR.PF has visible phenotype, suppressible by Ras85D^UAS.cKa, Scer\GAL4^GMR.PF

(Lee et al., 2002)

PDZ-GEF^EP388, Scer\GAL4^GMR.PF has visible phenotype, suppressible by R[+]/Rap1^rvB1

(Lee et al., 2002)

PDZ-GEF^EP388, Scer\GAL4^GMR.PF has visible phenotype, suppressible by Raf¹¹/phl[+]

(Lee et al., 2002)

NOT suppressed by

Statement

Reference

PDZ-GEF^EP388, Scer\GAL4^GMR.PF has visible phenotype, non-suppressible by Ras85D^e1B

(Lee et al., 2002)

NOT Suppressor of

Statement

Reference

PDZ-GEF^EP388, Scer\GAL4^GMR.PF is a non-suppressor of lethal phenotype of Rap1^S17A.UAS, Scer\GAL4^GMR.PF

(Dupuy et al., 2005)

Scer\GAL4^GMR.PF, PDZ-GEF^EP388/C3G^UAS.Tag:MYC is a non-suppressor of lethal phenotype of Rap1^S17A.UAS, Scer\GAL4^GMR.PF

(Dupuy et al., 2005)

Phenotype Manifest In

Suppressed by

Statement

Reference

PDZ-GEF^EP388, Scer\GAL4^srp has embryonic/larval plasmatocyte phenotype, suppressible by Rap1^P5709

(Huelsmann et al., 2006)

PDZ-GEF^EP388, Scer\GAL4^srp has larval ventral nerve cord phenotype, suppressible by Rap1^P5709

(Huelsmann et al., 2006)

PDZ-GEF^EP388, Scer\GAL4^srp has embryonic/larval plasmatocyte phenotype, suppressible by mys¹¹

(Huelsmann et al., 2006)

PDZ-GEF^EP388, Scer\GAL4^GMR.PF has eye phenotype, suppressible by Ras85D^UAS.cKa, Scer\GAL4^GMR.PF

(Lee et al., 2002)

PDZ-GEF^EP388, Scer\GAL4^GMR.PF has eye photoreceptor cell | ectopic phenotype, suppressible by R[+]/Rap1^rvB1

(Lee et al., 2002)

PDZ-GEF^EP388, Scer\GAL4^GMR.PF has ommatidium phenotype, suppressible by Raf¹¹/phl[+]

(Lee et al., 2002)

PDZ-GEF^EP388, Scer\GAL4^GMR.PF has eye photoreceptor cell | ectopic phenotype, suppressible by Raf¹¹/phl[+]

(Lee et al., 2002)

PDZ-GEF^EP388, Scer\GAL4^GMR.PF has eye phenotype, suppressible by Raf¹¹/phl[+]

(Lee et al., 2002)

PDZ-GEF^EP388, Scer\GAL4^GMR.PF has ommatidium phenotype, suppressible by Dsor1[+]/Dsor1^LH110

(Lee et al., 2002)

PDZ-GEF^EP388, Scer\GAL4^GMR.PF has eye photoreceptor cell | ectopic phenotype, suppressible by Dsor1[+]/Dsor1^LH110

(Lee et al., 2002)

PDZ-GEF^EP388, Scer\GAL4^GMR.PF has eye phenotype, suppressible by Dsor1[+]/Dsor1^LH110

(Lee et al., 2002)

PDZ-GEF^EP388, Scer\GAL4^GMR.PF has ommatidium phenotype, suppressible by rl[+]/rl¹

(Lee et al., 2002)

PDZ-GEF^EP388, Scer\GAL4^GMR.PF has eye photoreceptor cell | ectopic phenotype, suppressible by rl[+]/rl¹

(Lee et al., 2002)

PDZ-GEF^EP388, Scer\GAL4^GMR.PF has eye phenotype, suppressible by rl[+]/rl¹

(Lee et al., 2002)

PDZ-GEF^EP388, Scer\GAL4^GMR.PF has ommatidium phenotype, suppressible by Ras85D^UAS.cKa, Scer\GAL4^GMR.PF

(Lee et al., 2002)

PDZ-GEF^EP388, Scer\GAL4^GMR.PF has eye photoreceptor cell | ectopic phenotype, suppressible by Ras85D^UAS.cKa, Scer\GAL4^GMR.PF

(Lee et al., 2002)

NOT suppressed by

Statement

Reference

PDZ-GEF^EP388, Scer\GAL4^GMR.PF has eye photoreceptor cell | ectopic phenotype, non-suppressible by Ras85D^e1B

(Lee et al., 2002)

PDZ-GEF^EP388, Scer\GAL4^GMR.PF has eye phenotype, non-suppressible by Ras85D^e1B

(Lee et al., 2002)

Additional Comments

Genetic Interactions

Statement

Reference

The cell shape changes and protrusion network formation phenotype of macrophages expressing Gef26^EP388 under the control of Scer\GAL4^srp is substantially rescued in R^P5709 mutants.

Coexpression of Gef26^EP388 and R^{N17.Scer\UAS.T:Hsap\MYC} in macrophages, under the control of Scer\GAL4^srp, results in a substantial suppression of the R^{N17.Scer\UAS.T:Hsap\MYC} macrophage phenotype. These embryos show only a minimal gap in the distribution of the macrophages around the ventral nerve cord at stage 14, and the macrophages form larger protrusions than in cells expressing only R^{N17.Scer\UAS.T:Hsap\MYC}.

The macrophage phenotype of Scer\GAL4^srp>Gef26^EP388 embryos is suppressed by a mys¹¹ background; these macrophages have the appearance of wild-type cells regarding size and protrusions.

(Huelsmann et al., 2006)

The lethality caused by expression of R^{S17A.Scer\UAS} under the control of Scer\GAL4^GMR.PF is not rescued by co-expression of Gef26^EP388.

(Dupuy et al., 2005)

Flies which are co-expressing Ras85D^Scer\UAS.cKa and Gef26^EP388 under the control of Scer\GAL4^GMR.PF have a rather normal eye, with a normal ommatidial array and photoreceptor structure.

The rough eye phenotype, irregular ommatidial array and increased photoreceptor cell number caused by expression of Gef26^EP388 under the control of Scer\GAL4^GMR.PF are strongly suppressed by phl¹¹/+, Dsor1^LH110/+ or rl¹/+.

(Lee et al., 2002)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (7)

Reported As

Symbol Synonym

EP(2)0388

(Wang et al., 2006)

Gef26²

(Singh et al., 2006, Wang et al., 2006)

Gef26^EP388

PDZ-GEF^EP388

dPDZ-GEF²

(Lee et al., 2002)

dizzy^EP388

(Huelsmann et al., 2006)

dizzy^EP

(Huelsmann et al., 2006)

Name Synonyms

Secondary FlyBase IDs

References (5)

Report Sections

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