FlyBase Allele Report: Dmel\nvd[RNAi.1.UAS]

General Information

Symbol

Dmel\nvd^RNAi.1.UAS

Species

D. melanogaster

Name

FlyBase ID

FBal0193613

Feature type

allele

Associated gene

Dmel\nvd

Associated Insertion(s)

Carried in Construct

P{UAS-nvd.RNAi.1}

Also Known As

UAS-nvd-Dm-IR-1

Key Links

Transgenic product class

RNAi_reagent

(Yoshiyama et al., 2006)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Yoshiyama et al., 2006)

Mutagen

in vitro construct

(Yoshiyama et al., 2006)

Progenitor genotype

Carried in construct

P{UAS-nvd.RNAi.1}

Cytology

Description

UASt regulatory sequences drive expression of a nvd genomic DNA-cDNA inverted repeat. This repeat comprises genomic nvd sequences (nucleotides 488-1131 which correspond to part of the fourth exon and the entire fourth intron) fused to an inverted nvd cDNA fragment corresponding to the same region.

(Yoshiyama et al., 2006)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

nvd

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal developmental rate | first instar larval stage, with Scer\GAL4^2-286

(Yoshiyama et al., 2006)

auxotroph, with Scer\GAL4^2-286

(Lang et al., 2012)

lethal - all die before end of first instar larval stage, with Scer\GAL4^2-286

(Yoshiyama-Yanagawa et al., 2011, Yoshiyama et al., 2006)

lethal - all die before end of larval stage, with Scer\GAL4⁵⁰¹⁵

(Yoshiyama et al., 2006)

some die during first instar larval stage, with Scer\GAL4^2-286

(Yoshiyama-Yanagawa et al., 2011, Yoshiyama et al., 2006)

some die during larval stage, with Scer\GAL4⁵⁰¹⁵

(Yoshiyama et al., 2006)

viable, with Scer\GAL4^Akh.PL

(Yoshiyama et al., 2006)

viable, with Scer\GAL4^Aug21

(Yoshiyama et al., 2006)

viable, with Scer\GAL4^btl.PS

(Yoshiyama et al., 2006)

viable, with Scer\GAL4^dpp.blk1

(Yoshiyama et al., 2006)

viable, with Scer\GAL4^elav-C155

(Yoshiyama et al., 2006)

viable, with Scer\GAL4^Lsp2.PH

(Yoshiyama et al., 2006)

viable, with Scer\GAL4^sca-537.4

(Yoshiyama et al., 2006)

viable, with Scer\GAL4^tsh-Gal4-1

(Yoshiyama et al., 2006)

Phenotype Manifest In

Detailed Description

Statement

Reference

Ovaries from virgin females expressing nvd^{dsRNA.IR-1.UAS} under the control of Scer\GAL4^C587 do not show changes in the number of germline stem cells in the germarium, as compared to controls.

(Ameku et al., 2018)

Flies expressing nvd^{dsRNA.IR-1.Scer\UAS} under the control of Scer\GAL4^2-286 do not survive to adulthood on regular fly food or food supplemented with lathosterol, but are viable on food supplemented with 7-dehydrocholesterol.

(Lang et al., 2012)

Following hatching, first instar larvae expressing nvd^{dsRNA.IR-1.Scer\UAS} under the control of Scer\GAL4^2-286 (resulting in nvd RNAi) appear normal in terms of morphology and behaviour. However, their development then arrests and they fail to moult. At around 72 hours AEL, most larvae gradually cease feeding and become stationary. 98% die before 108 hours AEL and are still first instar larva, judging from the mouth-hook morphology.

The prolonged larval phenotype of Scer\GAL4^2-286>nvd^{dsRNA.IR-1.Scer\UAS} animals can be partially rescued by feeding the larvae with active ecdysteroid (20E) at 30-42 hours AEL. Rescued larvae are able to progress to the second instar, but then remain in this stage for a prolonged time and die. If these larvae are then fed again with 20E at 54-66 hours AEL, they progress to the third instar but do not pupate. Rescued second and third instar larvae do not grow beyond the size of wild-type larvae, even though they remain in these stages for a prolonged time (3-4 days following molting). Feeding the rescued third instar larvae with 20E after 78 hours AEL allows them to develop into pupae and a number of these pupae complete metamorphosis and become fertile adults, although most die within one week of eclosion.

Feeding Scer\GAL4^2-286>nvd^{dsRNA.IR-1.Scer\UAS} larvae with 7-dehydrocholesterol at 40 hours AEL results in a full rescue of the prolonged larval phenotype. Feeding with cholesterol at 38 hours AEL results in a partial rescue with around 47% of larvae molting to the second instar. These rescued larvae fail to develop into the third instar but do survive for about 3 weeks and grow to almost the same size as wild-type third instar larvae.

(Yoshiyama et al., 2006)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Scer\GAL4^2-286, nvd^RNAi.1.UAS has auxotroph phenotype, suppressible by Dpac\nvd^4mut.UAS, Scer\GAL4^2-286

(Lang et al., 2012)

Scer\GAL4^2-286, nvd^RNAi.1.UAS has lethal | first instar larval stage phenotype, suppressible by Bmor\nvd^UAS.Tag:HA, Scer\GAL4^2-286

(Yoshiyama-Yanagawa et al., 2011)

Scer\GAL4^2-286, nvd^RNAi.1.UAS has lethal | first instar larval stage phenotype, suppressible by Cele\daf-36^UAS.Tag:HA, Scer\GAL4^2-286

(Yoshiyama-Yanagawa et al., 2011)

Scer\GAL4^2-286, nvd^RNAi.1.UAS has lethal | first instar larval stage phenotype, suppressible by Drer\zgc:92275^UAS.Tag:HA, Scer\GAL4^2-286

(Yoshiyama-Yanagawa et al., 2011)

Scer\GAL4^2-286, nvd^RNAi.1.UAS has lethal | first instar larval stage phenotype, suppressible by Scer\GAL4^2-286/Xlae\nvd^UAS.Tag:HA

(Yoshiyama-Yanagawa et al., 2011)

NOT suppressed by

Statement

Reference

Scer\GAL4^2-286, nvd^RNAi.1.UAS has auxotroph phenotype, non-suppressible by Dpac\nvd^UAS.cLa, Scer\GAL4^2-286

(Lang et al., 2012)

Scer\GAL4^2-286, nvd^RNAi.1.UAS has lethal | first instar larval stage phenotype, non-suppressible by Bmor\nvd^{H190A.UAS.Tag:HA}, Scer\GAL4^2-286

(Yoshiyama-Yanagawa et al., 2011)

Scer\GAL4^2-286, nvd^RNAi.1.UAS has lethal | first instar larval stage phenotype, non-suppressible by Bmor\nvd^{H282A.UAS.Tag:HA}, Scer\GAL4^2-286

(Yoshiyama-Yanagawa et al., 2011)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Co-expression of Dpac\nvd^Scer\UAS.cLa rescues survival to adulthood of flies expressing nvd^{dsRNA.IR-1.Scer\UAS} under the control of Scer\GAL4^2-286 on food supplemented with lathosterol, but there is no rescue of survival on regular fly food. These flies survive significantly better on food supplemented with lathosterol compared to food supplemented with 7-dehydrocholesterol.

Co-expression of Dpac\nvd^{4mut.Scer\UAS} rescues survival to adulthood of flies expressing nvd^{dsRNA.IR-1.Scer\UAS} under the control of Scer\GAL4^2-286 on both regular fly food and food supplemented with lathosterol.

(Lang et al., 2012)

The lethality caused by expression of nvd^{dsRNA.IR-1.Scer\UAS} under the control of Scer\GAL4^2-286 is suppressed by co-expression of either Bmor\nvd^{Scer\UAS.T:Ivir\HA1}, Cele\daf-36^{Scer\UAS.T:Ivir\HA1}, Brer\nvd^{Scer\UAS.T:Ivir\HA1} or Xlae\nvd^{Scer\UAS.T:Ivir\HA1}.

The lethality caused by expression of nvd^{dsRNA.IR-1.Scer\UAS} under the control of Scer\GAL4^2-286 is not suppressed by co-expression of either Bmor\nvd^{H190A.Scer\UAS.T:Ivir\HA1} or Bmor\nvd^{H282A.Scer\UAS.T:Ivir\HA1}.

(Yoshiyama-Yanagawa et al., 2011)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Comments

Transcription from the P{UAS-nvd.IR-1} produces nvd dsRNA, resulting in dsRNA interference (RNAi) of the nvd gene.

(Yoshiyama et al., 2006)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (5)

Reported As

Symbol Synonym

nvd^RNAi.1.UAS

nvd^{RNAi.IR-1.UAS}

nvd^RNAi

(Ameku et al., 2018)

nvd^{dsRNA.IR-1.Scer\UAS}

nvd^{dsRNA.IR-1.UAS}

Name Synonyms

Secondary FlyBase IDs

References (7)

Report Sections

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