FlyBase Allele Report: Dmel\Gαo[UAS.cKa]

General Information

Symbol

Dmel\Gαo^UAS.cKa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Katanaev

FlyBase ID

FBal0183481

Feature type

allele

Associated gene

Dmel\Gαo

Associated Insertion(s)

Carried in Construct

P{UAS(-FRT)Gαo.K}

Also Known As

UAS-Go, UAS-Gαo

Key Links

Transgenic product class

wild_type

(Katanaev et al., 2005)

Mutagen

FLPase

Nature of the Allele

Transgenic product class

wild_type

(Katanaev et al., 2005)

Mutagen

FLPase

(Katanaev et al., 2005)

Progenitor genotype

Gαo^FRT.UAS

(Katanaev et al., 2005)

Carried in construct

P{UAS(-FRT)Gαo.K}

Cytology

Description

The FRT cassette has been excised, juxtaposing the UAS regulatory sequences and wild-type Gαo class II isoform coding region.

(Katanaev et al., 2005)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

Gαo

Also carries

FRT

recombinase target site

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal cell polarity, with Scer\GAL4^Bx-MS1096

(Katanaev and Tomlinson, 2006)

abnormal mitotic cell cycle, with Scer\GAL4^Bx-MS1096

(Katanaev and Tomlinson, 2006)

abnormal neuroanatomy, with Scer\GAL4^repo

(Schwabe et al., 2005)

abnormal planar polarity, with Scer\GAL4^Bx-MS1096

(Katanaev and Tomlinson, 2006, Katanaev et al., 2005)

Phenotype Manifest In

medial triple row & eo neuron, with Scer\GAL4^pnr-MD237

(Katanaev and Tomlinson, 2006)

medial triple row & eo sensory structure, with Scer\GAL4^pnr-MD237

(Katanaev and Tomlinson, 2006)

medial triple row & sensory organ cell, with Scer\GAL4^pnr-MD237

(Katanaev and Tomlinson, 2006)

medial triple row & socket, with Scer\GAL4^pnr-MD237

(Katanaev and Tomlinson, 2006)

pupa, with Scer\GAL4^CCAP.PP

(Kim et al., 2015)

wing hair | increased number, with Scer\GAL4^Bx-MS1096

(Katanaev et al., 2005)

wing margin bristle, with Scer\GAL4^Bx-MS1096

(Katanaev and Tomlinson, 2006)

Detailed Description

Statement

Reference

Expression of Gαo^Scer\UAS.cKa under the control of Scer\GAL4^CCAP.PP increases duration of pupal pre-ecdysis behavior stage, while expression under two copies of Scer\GAL4^Lk.PM has no significant effect on the stage length.

(Kim et al., 2015)

Flies expressing G-oα47A^Scer\UAS.cKa under the control of Scer\GAL4^pnr-MD237 exhibit defective wing margin stout bristles. Defective bristles exhibit a range of phenotypes, they may contain: two sockets/two hairs, one socket/three hairs, one socket/two hairs, two sockets/one hair, no socket/two hairs, no socket/one hair, or one or two sockets/no hair. Flies expressing G-oα47A^Scer\UAS.cKa under the control of Scer\GAL4^Bx-MS1096 exhibit defects in approximately 1.5% of wing margin bristles.

(Katanaev and Tomlinson, 2006)

Expression of G-oα47A^Scer\UAS.cKa under the control of Scer\GAL4^Bx-MS1096 induces multiple wing hairs.

(Katanaev et al., 2005)

Expression of G-oα47A^Scer\UAS.cKa in glia of embryos, driven by Scer\GAL4^repo, causes defects in the formation of the blood-brain barrier, as assessed by the penetrance of a fluorescent dye into the nerve cord.

(Schwabe et al., 2005)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Gαo^UAS.cKa, Scer\GAL4^Bx-MS1096 has abnormal planar polarity phenotype, enhanceable by raps[+]/pins¹⁹³

(Katanaev and Tomlinson, 2006)

Gαo^UAS.cKa, Scer\GAL4^Bx-MS1096 has abnormal cell polarity phenotype, enhanceable by raps[+]/pins^Δ50

(Katanaev and Tomlinson, 2006)

Gαo^UAS.cKa, Scer\GAL4^Bx-MS1096 has abnormal planar polarity phenotype, enhanceable by fz^UAS.cSa, Scer\GAL4^Bx-MS1096

(Katanaev et al., 2005)

NOT Enhanced by

Statement

Reference

Gαo^UAS.cKa, Scer\GAL4^Bx-MS1096 has abnormal cell polarity phenotype, non-enhanceable by loco[+]/loco^M1

(Katanaev and Tomlinson, 2006)

Gαo^UAS.cKa, Scer\GAL4^Bx-MS1096 has abnormal planar polarity phenotype, non-enhanceable by loco[+]/loco^M1

(Katanaev and Tomlinson, 2006)

Gαo^UAS.cKa, Scer\GAL4^Bx-MS1096 has abnormal mitotic cell cycle phenotype, non-enhanceable by loco[+]/loco^M1

(Katanaev and Tomlinson, 2006)

Suppressed by

Statement

Reference

Gαo^UAS.cKa, Scer\GAL4^Bx-MS1096 has abnormal mitotic cell cycle phenotype, suppressible by Ggamma1[+]/Gγ1^k08017

(Katanaev and Tomlinson, 2006)

Gαo^UAS.cKa, Scer\GAL4^Bx-MS1096 has abnormal planar polarity phenotype, suppressible by fz^unspecified

(Katanaev et al., 2005)

NOT suppressed by

Statement

Reference

Gαo^UAS.cKa, Scer\GAL4^Bx-MS1096 has abnormal cell polarity phenotype, non-suppressible by loco[+]/loco^M1

(Katanaev and Tomlinson, 2006)

Gαo^UAS.cKa, Scer\GAL4^Bx-MS1096 has abnormal planar polarity phenotype, non-suppressible by loco[+]/loco^M1

(Katanaev and Tomlinson, 2006)

Gαo^UAS.cKa, Scer\GAL4^Bx-MS1096 has abnormal mitotic cell cycle phenotype, non-suppressible by loco[+]/loco^M1

(Katanaev and Tomlinson, 2006)

Enhancer of

Statement

Reference

Gαo^UAS.cKa, Scer\GAL4^Bx-MS1096 is an enhancer of abnormal planar polarity phenotype of Scer\GAL4^Bx-MS1096, kermit^UAS.cLa

(Lin and Katanaev, 2013)

Gαo^UAS.cKa/Scer\GAL4^Bx-MS1096 is an enhancer of abnormal mitotic cell cycle phenotype of pins¹⁹³

(Katanaev and Tomlinson, 2006)

Gαo^UAS.cKa/Scer\GAL4^Bx-MS1096 is an enhancer of abnormal mitotic cell cycle phenotype of pins^Δ50

(Katanaev and Tomlinson, 2006)

Gαo^UAS.cKa, Scer\GAL4^Bx-MS1096 is an enhancer of abnormal planar polarity phenotype of Scer\GAL4^Bx-MS1096, fz^UAS.cSa

(Katanaev et al., 2005)

Phenotype Manifest In

Enhanced by

Statement

Reference

Gαo^UAS.cKa, Scer\GAL4^Bx-MS1096 has wing margin bristle phenotype, enhanceable by fz2^UAS.GFP, Scer\GAL4^Bx-MS1096

(Katanaev and Tomlinson, 2006)

Gαo^UAS.cKa, Scer\GAL4^Bx-MS1096 has wing hair | increased number phenotype, enhanceable by fz^UAS.cSa, Scer\GAL4^Bx-MS1096

(Katanaev et al., 2005)

Gαo^UAS.cKa, Scer\GAL4^Bx-MS1096 has wing hair phenotype, enhanceable by fz^UAS.cSa, Scer\GAL4^Bx-MS1096

(Katanaev et al., 2005)

Suppressed by

Statement

Reference

Gαo^UAS.cKa, Scer\GAL4^Bx-MS1096 has wing margin bristle phenotype, suppressible by Ggamma1[+]/Gγ1^k08017

(Katanaev and Tomlinson, 2006)

Gαo^UAS.cKa, Scer\GAL4^Bx-MS1096 has wing margin bristle phenotype, suppressible by fz^unspecified/fz^unspecified

(Katanaev and Tomlinson, 2006)

Gαo^UAS.cKa, Scer\GAL4^Bx-MS1096 has wing hair | increased number phenotype, suppressible by fz^unspecified

(Katanaev et al., 2005)

Gαo^UAS.cKa, Scer\GAL4^Bx-MS1096 has wing hair phenotype, suppressible by fz^unspecified

(Katanaev et al., 2005)

Enhancer of

Statement

Reference

Gαo^UAS.cKa, Scer\GAL4^Bx-MS1096 is an enhancer of wing hair phenotype of Scer\GAL4^Bx-MS1096, kermit^UAS.cLa

(Lin and Katanaev, 2013)

Gαo^UAS.cKa, Scer\GAL4^Bx-MS1096 is an enhancer of wing hair | increased number phenotype of Scer\GAL4^Bx-MS1096, kermit^UAS.cLa

(Lin and Katanaev, 2013)

Gαo^UAS.cKa, Scer\GAL4^Bx-MS1096 is an enhancer of wing margin bristle | ectopic phenotype of Scer\GAL4^Bx-MS1096, fz2^UAS.GFP

(Katanaev and Tomlinson, 2006)

Gαo^UAS.cKa, Scer\GAL4^Bx-MS1096 is an enhancer of wing hair | increased number phenotype of Scer\GAL4^Bx-MS1096, fz^UAS.cSa

(Katanaev et al., 2005)

Gαo^UAS.cKa, Scer\GAL4^Bx-MS1096 is an enhancer of wing hair phenotype of Scer\GAL4^Bx-MS1096, fz^UAS.cSa

(Katanaev et al., 2005)

Additional Comments

Genetic Interactions

Statement

Reference

Co-expression of Gαo^Scer\UAS.cKa enhances the wing defects caused by expressing kermit^Scer\UAS.cLa under the control of Scer\GAL4^Bx-MS1096, significantly increasing the number of cells producing multiple hairs and producing severe reorientation of hairs in some wing regions. The wing cells often produce 3 hairs in the double mutant wings.

(Lin and Katanaev, 2013)

Overexpressed G-oα47A^Scer\UAS.cKa, under the control of Scer\GAL4^Bx-MS1096 induces division defects that are attenuated by the removal of one copy of Gγ1^k08017. Overexpression of G-oα47A^Scer\UAS.cKa, under the control of Scer\GAL4^Bx-MS1096 in a raps^Δ50 or raps¹⁹³ heterozygous background results in a significant enhancement of the asymmetric division defects observed in G-oα47A^Scer\UAS.cKa Scer\GAL4^Bx-MS1096 mutants. The effects of overexpression of G-oα47A^Scer\UAS.cKa are suppressed in fz^unspecified homozygous wings. The effects of overexpression of G-oα47A^Scer\UAS.cKa under the control of Scer\GAL4^Bx-MS1096 are attenuated in Gγ1^k08017 heterozygotes. Coexpression of fz2^{Scer\UAS.T:Avic\GFP} and G-oα47A^Scer\UAS.cKa under the control of Scer\GAL4^Bx-MS1096 results in mutual enhancement of their respective phenotypes. No modification of the G-oα47A^Scer\UAS.cKa overexpression phenotype (under Scer\GAL4^Bx-MS1096 control) is observed in loco^M1 heterozygous mutants.

(Katanaev and Tomlinson, 2006)

G-oα47A^Scer\UAS.cKa and fz^Scer\UAS.cSa act synergistically when co-expressed using Scer\GAL4^Bx-MS1096, resulting in severe defects in wing hair polarity and many cells with multiple wing hairs. The frequency of multiple wing hairs induced by expression of G-oα47A^Scer\UAS.cKa under the control of Scer\GAL4^Bx-MS1096 is reduced if the flies also carry fz^unspecified.

(Katanaev et al., 2005)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Partially rescues

Scer\GAL4^NP3200/Gαo^UAS.cKa partially rescues Gαo^NP3200

(Bredendiek et al., 2011)

Comments

Expression of G-oα47A^Scer\UAS.cKa under the control of Scer\GAL4^{G-oα47A-NP3200} partially rescues the reduced spike rate in response to sucrose that is seen in the L-type sensilla of G-oα47A^NP3200 heterozygotes.

Expression of G-oα47A^Scer\UAS.cKa under the control of Scer\GAL4^{G-oα47A-NP3200} rescues the impaired ability of G-oα47A^NP3200 heterozygotes to distinguish between 5mM and 2mM sucrose in a 2-choice preference assay.

(Bredendiek et al., 2011)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

G-oα47A^Scer\UAS.cKa

Gαo^Scer\UAS.cKa

Gαo^UAS.cKa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Katanaev

(Katanaev et al., 2005)

Secondary FlyBase IDs

References (11)

Report Sections

Open Close