The L-type sensilla of heterozygous flies show a significantly reduced spike rate in response to sucrose (over a range of concentrations extending from 20 to 500mM) compared to wild-type control sensilla. The mutant L-type sensilla show a normal electrophysiological response to maltose and trehalose, but show a reduced spike rate in response to glucose or fructose.
Heterozygous flies have a reduced ability to distinguish between 5mM and 2mM sucrose in a 2-choice preference assay compared to wild-type controls. The heterozygous flies prefer 13mM maltose or 75 mM trehalose over 5mM sucrose in a 2-choice preference assay, in contrast to wild-type flies which have no preference for 13mM maltose over 5mM sucrose or for 75 mM trehalose over 5mM sucrose.
Only 24% of heterozygotes display a proboscis extension reflex in response to 100mM sucrose, compared to 93% of wild-type controls.
Heterozygotes show a normal avoidance response to caffeine and to quinine in a 2-choice preference assay. The S6 sensilla of these flies show a normal electrophysiological response to caffeine and to quinine.
GαoNP3200 is partially rescued by Scer\GAL4NP3200/GαoUAS.cKa
Expression of G-oα47AScer\UAS.cKa under the control of Scer\GAL4G-oα47A-NP3200 partially rescues the reduced spike rate in response to sucrose that is seen in the L-type sensilla of G-oα47ANP3200 heterozygotes.
Expression of G-oα47AScer\UAS.cKa under the control of Scer\GAL4G-oα47A-NP3200 rescues the impaired ability of G-oα47ANP3200 heterozygotes to distinguish between 5mM and 2mM sucrose in a 2-choice preference assay.
