FlyBase Allele Report: Dmel\sti[GS9053]

General Information

Symbol

Dmel\sti^GS9053

Species

D. melanogaster

Name

FlyBase ID

FBal0177615

Feature type

allele

Associated gene

Dmel\sti

Associated Insertion(s)

P{GSV6}sti^GS9053

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

Nature of the Allele

Allele class

Mutagen

Progenitor genotype

Associated Insertion(s)

P{GSV6}sti^GS9053

Cytology

Description

Insertion oriented to drive expression in the sense direction starting upstream of the coding region.

(Gregory et al., 2007)

Allele components

Component

Use(s)

Inserted element

P{GSV6}

misexpression element

Regulatory region(s)

UASt

binary expression system - regulatory region

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

3L:12,514,736..12,514,736

(Bassi et al., 2011, Gregory et al., 2007, Shandala et al., 2004)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal cytokinesis (with Df(3L)iro-2)

(Shandala et al., 2004)

lethal | pupal stage

(Shandala et al., 2004)

lethal | pupal stage (with Df(3L)iro-2)

(Shandala et al., 2004)

lethal | pupal stage (with sti^KG01697)

(Shandala et al., 2004)

partially lethal - majority die | larval stage

(Shandala et al., 2004)

partially lethal - majority die | larval stage (with Df(3L)iro-2)

(Shandala et al., 2004)

partially lethal - majority die | larval stage (with sti^KG01697)

(Shandala et al., 2004)

Phenotype Manifest In

contractile ring (with Df(3L)iro-2)

(Bassi et al., 2011)

embryonic/larval brain (with Df(3L)iro-2)

(Shandala et al., 2004)

embryonic peripheral nervous system & neuron (with Df(3L)iro-2)

(Shandala et al., 2004)

imaginal disc (with Df(3L)iro-2)

(Shandala et al., 2004)

presumptive embryonic/larval peripheral nervous system (with Df(3L)iro-2)

(Shandala et al., 2004)

Detailed Description

Statement

Reference

Contractile ring dynamics in sti^GS9053/Df(3L)iro-2 mutant neuroblasts are similar to controls, albeit less uniform, indicating that the defects in contractile ring organization observed in the absence of sti occur after and not during cleavage furrow ingression.

(Bassi et al., 2011)

Expression of sti^GS9053 under the control of Scer\GAL4^GMR.PF results in a wild type eye.

(Gregory et al., 2007)

Stage 16 sti^GS9053/Df(3L)iro-2 embryos have a modestly disrupted peripheral nervous system (PNS) with some disorganisation of clusters and defects in axonal projections. There is also a reduction in the numbers of PNS neurons in these embryos, and concomitant appearance of binucleate neurons (about 15% of total neurons are binucleate). About 1/4 of cells in the brains of third instar sti^GS9053/Df(3L)iro-2 larvae have massively enlarged nuclei. Chromosome preparations from these brains show tetraploid metaphase figures and some massively hyperploid cells. As chromosomes seem to separate normally in all except the most polyploid of cells, the defect appears to be in cytokinesis, rather than some other aspect of mitosis. These larvae also exhibit a variable reduction in imaginal disc size. This is associated with an increase in apoptosis in the imaginal discs and a decrease in numbers of cells expressing mitotic markers.

(Shandala et al., 2004)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

sti^GS9053/Scer\GAL4^en-e16E is an enhancer of abnormal mitotic cell cycle | third instar larval stage phenotype of Scer\GAL4^en-e16E, pbl^RNAi.UAS

(Gregory et al., 2007)

sti^GS9053/Scer\GAL4^GMR.PF is an enhancer of visible phenotype of Scer\GAL4^GMR.PF, pbl^{ΔDH497-549.UAS}

(Gregory et al., 2007)

Suppressor of

Statement

Reference

sti^GS9053/Scer\GAL4^GMR.PF is a suppressor | partially of visible phenotype of Scer\GAL4^GMR.PF, pbl^UAS.cPa

(Gregory et al., 2007)

Phenotype Manifest In

Enhanced by

Statement

Reference

sti^GS9053/Df(3L)iro-2 has embryonic peripheral nervous system & neuron phenotype, enhanceable by pbl[+]/pbl³

(Shandala et al., 2004)

Enhancer of

Statement

Reference

sti^GS9053/Scer\GAL4^GMR.PF is an enhancer of eye phenotype of Scer\GAL4^GMR.PF, pbl^{ΔDH497-549.UAS}

(Gregory et al., 2007)

NOT Enhancer of

Statement

Reference

sti^GS9053/Scer\GAL4^en-e16E is a non-enhancer of wing | posterior phenotype of Scer\GAL4^en-e16E, pbl^RNAi.UAS

(Gregory et al., 2007)

sti^GS9053/Scer\GAL4^en-e16E is a non-enhancer of wing | posterior & wing hair phenotype of Scer\GAL4^en-e16E, pbl^RNAi.UAS

(Gregory et al., 2007)

Suppressor of

Statement

Reference

sti^GS9053/Scer\GAL4^GMR.PF is a suppressor | partially of eye phenotype of Scer\GAL4^GMR.PF, pbl^UAS.cPa

(Gregory et al., 2007)

NOT Suppressor of

Statement

Reference

sti^GS9053/Scer\GAL4^en-e16E is a non-suppressor of wing | posterior phenotype of Scer\GAL4^en-e16E, pbl^RNAi.UAS

(Gregory et al., 2007)

sti^GS9053/Scer\GAL4^en-e16E is a non-suppressor of wing | posterior & wing hair phenotype of Scer\GAL4^en-e16E, pbl^RNAi.UAS

(Gregory et al., 2007)

sti^GS9053/Scer\GAL4^en-e16E is a non-suppressor of wing hair | increased number phenotype of Scer\GAL4^en-e16E, pbl^RNAi.UAS

(Shandala et al., 2004)

sti^GS9053/Scer\GAL4^en-e16E is a non-suppressor of wing blade posterior compartment phenotype of Scer\GAL4^en-e16E, pbl^RNAi.UAS

(Shandala et al., 2004)

Additional Comments

Genetic Interactions

Statement

Reference

The The reduction in PNS neuron numbers and the percentage of binucleate PNS neurons in sti^GS9053/Df(3L)iro-2 embryos are both enhanced by pbl³/+. When cell death is inhibited in sti^GS9053/Df(3L)iro-2 third instar discs by the presence of BacA\p35^Scer\UAS.cHa, multinucleate cells accumulate.

(Shandala et al., 2004)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

sti^GS9053/Df(3L)iro-2 is rescued by Scer\GAL4^da.G32, sti^GS9053

(Shandala et al., 2004)

Rescues

Scer\GAL4^da.G32, sti^GS9053 rescues sti^GS9053/Df(3L)iro-2

(Shandala et al., 2004)

Comments

Driving expression of sti using Scer\GAL4^da.G32 in sti^GS9053/Df(3L)iro-2 animals (via P{GSV6} Scer\UAS sequences), rescues lethality.

(Shandala et al., 2004)

Images (0)

Mutant

Wild-type

Stocks (1)

Kyoto

201488

y¹ w^67c23; P{GSV6}sti^GS9053/TM3, Sb¹ Ser¹

Notes on Origin

Discoverer

Toshiro Aigaki

(Shandala et al., 2004)

Comments

Lethality over Df(3L)iro-2 is revertable with Δ2-3. However, original stock carries 2nd site lethal on 3R.

(Shandala et al., 2004)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

cit²

(Shandala et al., 2004)

sti^GS9053

(Tran et al., 2019)

Name Synonyms

Secondary FlyBase IDs

References (4)

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