FlyBase Allele Report: Dmel\Vps35[EY14200]

General Information

Symbol

Dmel\Vps35^EY14200

Species

D. melanogaster

Name

FlyBase ID

FBal0161256

Feature type

allele

Associated gene

Dmel\Vps35

Associated Insertion(s)

P{EPgy2}Vps35^EY14200

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

P-element activity

Nature of the Allele

Allele class

Mutagen

P-element activity

(Gene Disruption Project members, 2001-)

Progenitor genotype

Associated Insertion(s)

P{EPgy2}Vps35^EY14200

Cytology

Description

Insertion 21bp upstream of the first and 190bp upstream of the second ATG site/

(Port et al., 2008)

Insertion in the 5' untranslated region.

(Korolchuk et al., 2007)

Allele components

Component

Use(s)

Inserted element

P{EPgy2}

misexpression element

Regulatory region(s)

UASp

binary expression system - regulatory region

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

2R:22,186,070..22,186,070 [+]

(Sanhueza et al., 2015, Linhart et al., 2014, Belenkaya et al., 2008, Port et al., 2008, Korolchuk et al., 2007, Gene Disruption Project members, 2001-)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates Parkinson's disease 8

modeled by Hsap\LRRK2^I2020T.UAS

(Linhart et al., 2014)

ameliorates amyotrophic lateral sclerosis type 8

modeled by Vap33^{P58S.UAS.Tag:FLAG}

(Sanhueza et al., 2015)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | larval stage

(Korolchuk et al., 2007)

abnormal neuroanatomy | larval stage (with Df(2R)ED3952)

(Korolchuk et al., 2007)

lethal | larval stage (with Df(2R)ED3952)

(Korolchuk et al., 2007)

lethal | larval stage | recessive

(Korolchuk et al., 2007)

lethal | pupal stage (with Df(2R)ED3952)

(Korolchuk et al., 2007)

lethal | pupal stage | recessive

(Korolchuk et al., 2007)

lethal | recessive

(Port et al., 2008)

melanotic mass phenotype | larval stage

(Korolchuk et al., 2007)

visible, with Scer\GAL4^GMR.PU

(Linhart et al., 2014)

Phenotype Manifest In

Detailed Description

Statement

Reference

Over-expression of Vps35^EY14200 driven by Scer\GAL4^GMR.PU causes a mild eye phenotype with occasional presence of black lesions (in around 8% of mutant flies, compared to 3% in controls). Expression of Vps35^EY14200 driven by Scer\GAL4^Ddc.PU does not cause significant locomotor deficits or changes in lifespan.

(Linhart et al., 2014)

Rare adult homozygous escapers are seen, but they are unable to move and die shortly after eclosion.

Homozygous larvae develop melanotic masses in the body cavity. The hemolymph of mutant larvae contains an increased number of plasmatocytes compared to controls and also contains significant numbers of lamellocytes (which are absent in wild-type larval haemolymph).

Vps35^EY14200/Df(2R)ED3952 larvae have an increased number of haemocytes compared to controls.

Primary cultured haemocytes from homozygous and Vps35^EY14200/Df(2R)ED3952 third instar larvae show reduced uptake of maleylated bovine serum albumin (mBSA) compared to wild-type haemocytes, indicating a defect in endocytosis.

Haemocytes isolated from mutant larvae often have increased numbers of lamellopodia compared with wild type and these often appear detached from the coverslip beneath them.

Boutons at the Vps35^EY14200 neuromuscular junction show no significant defect in uptake of the styryl dye FM1-43FX, during stimulation by 90mM K[+].

Neuromuscular junctions (NMJs) of homozygous larvae have many satellite boutons that protrude from larger boutons (these are rare in wild-type synapses). This results in a twofold increase in total bouton number per NMJ compared to wild type.

Vps35^EY14200/Df(2R)ED3952 NMJs have an increase in total bouton number per NMJ compared to wild type.

(Korolchuk et al., 2007)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Vps35^EY14200/Df(2R)ED3952 has abnormal neuroanatomy | larval stage phenotype, suppressible by Rac1^N17.UAS/Scer\GAL4^C57

(Korolchuk et al., 2007)

Vps35^EY14200 has abnormal neuroanatomy | larval stage phenotype, suppressible by wit^unspecified/wit[+]

(Korolchuk et al., 2007)

Vps35^EY14200 has abnormal neuroanatomy | larval stage phenotype, suppressible by Mad[+]/Mad¹⁰

(Korolchuk et al., 2007)

Vps35^EY14200/Df(2R)ED3952 has abnormal neuroanatomy | larval stage phenotype, suppressible by Rac1^N17.UAS/Scer\GAL4^elav.PLu

(Korolchuk et al., 2007)

NOT suppressed by

Statement

Reference

Vps35^EY14200 has abnormal neuroanatomy | larval stage phenotype, non-suppressible by fz2[+]/fz2^C1

(Korolchuk et al., 2007)

Suppressor of

Statement

Reference

Vps35^EY14200, Scer\GAL4^GMR.PU is a suppressor of visible phenotype of Hsap\LRRK2^I2020T.UAS, Scer\GAL4^GMR.PU

(Linhart et al., 2014)

Vps35^EY14200, Scer\GAL4^Ddc.PU is a suppressor of abnormal locomotor behavior | adult stage phenotype of Hsap\LRRK2^I2020T.UAS, Scer\GAL4^Ddc.PU

(Linhart et al., 2014)

Vps35^EY14200, Scer\GAL4^Ddc.PU is a suppressor of short lived phenotype of Hsap\LRRK2^I2020T.UAS, Scer\GAL4^Ddc.PU

(Linhart et al., 2014)

Vps35^EY14200, Scer\GAL4^Ddc.PU is a suppressor of abnormal locomotor behavior | adult stage phenotype of Hsap\LRRK2^Y1699C.UAS, Scer\GAL4^Ddc.PU

(Linhart et al., 2014)

Vps35^EY14200, Scer\GAL4^Ddc.PU is a suppressor of abnormal locomotor behavior | adult stage phenotype of Hsap\LRRK2^I1122V.UAS, Scer\GAL4^Ddc.PU

(Linhart et al., 2014)

Phenotype Manifest In

Suppressed by

Statement

Reference

Vps35^EY14200 has NMJ bouton phenotype, suppressible by wit^unspecified/wit[+]

(Korolchuk et al., 2007)

Vps35^EY14200 has NMJ bouton phenotype, suppressible by Mad[+]/Mad¹⁰

(Korolchuk et al., 2007)

Vps35^EY14200 has NMJ bouton phenotype, suppressible by Rac1^J11/Rac1[+]

(Korolchuk et al., 2007)

Vps35^EY14200/Df(2R)ED3952 has NMJ bouton phenotype, suppressible by Rac1^N17.UAS/Scer\GAL4^elav.PLu

(Korolchuk et al., 2007)

Vps35^EY14200/Df(2R)ED3952 has NMJ bouton phenotype, suppressible by Rac1^N17.UAS/Scer\GAL4^C57

(Korolchuk et al., 2007)

NOT suppressed by

Statement

Reference

Vps35^EY14200 has NMJ bouton phenotype, non-suppressible by fz2[+]/fz2^C1

(Korolchuk et al., 2007)

Suppressor of

Statement

Reference

Vps35^EY14200, Scer\GAL4^GMR.PU is a suppressor of eye | adult stage phenotype of Hsap\LRRK2^I2020T.UAS, Scer\GAL4^GMR.PU

(Linhart et al., 2014)

Additional Comments

Genetic Interactions

Statement

Reference

The increased bouton number at the neuromuscular junction that is seen in homozygous Vps35^EY14200 larvae is not suppressed by fz2^C1/+ but is suppressed by wit^unspecified/+ or Mad¹⁰/+.

The increased bouton number at the neuromuscular junction that is seen in Vps35^EY14200/Df(2R)ED3952 larvae is suppressed by Rac1^J11/+.

The increased bouton number at the neuromuscular junction that is seen in Vps35^EY14200/Df(2R)ED3952 larvae is suppressed by expression of Rac1^N17.Scer\UAS under the control of either Scer\GAL4^elav.PLu or Scer\GAL4^C57.

(Korolchuk et al., 2007)

Xenogenetic Interactions

Statement

Reference

Co-expression of Vps35^EY14200 significantly suppresses eye phenotypes (presence of black lesions in around 50% of flies), locomotor deficits and shortened lifespan in flies with expression of Hsap\LRRK2^{I2020T.Scer\UAS} driven by Scer\GAL4^GMR.PU at 29[o]C. Co-expression of Vps35^EY14200 significantly suppresses locomotor deficits in flies with expression of Hsap\LRRK2^{I1122V.Scer\UAS} or Hsap\LRRK2^{Y1699C.Scer\UAS} driven by Scer\GAL4^Ddc.PU.

(Linhart et al., 2014)

Complementation and Rescue Data

Rescued by

Vps35^EY14200/Df(2R)ED3952 is rescued by Scer\GAL4^Hml.PG, Vps35^EY14200

(Korolchuk et al., 2007)

Vps35^EY14200/Df(2R)ED3952 is rescued by Scer\GAL4^elav.PLu/Scer\GAL4^C57, Vps35^EY14200

(Korolchuk et al., 2007)

Partially rescued by

Vps35^EY14200/Df(2R)ED3952 is partially rescued by Scer\GAL4^elav.PLu, Vps35^EY14200

(Korolchuk et al., 2007)

Vps35^EY14200/Df(2R)ED3952 is partially rescued by Scer\GAL4^C57, Vps35^EY14200

(Korolchuk et al., 2007)

Rescues

Scer\GAL4^Hml.PG, Vps35^EY14200 rescues Vps35^EY14200/Df(2R)ED3952

(Korolchuk et al., 2007)

Scer\GAL4^elav.PLu/Scer\GAL4^C57, Vps35^EY14200 rescues Vps35^EY14200/Df(2R)ED3952

(Korolchuk et al., 2007)

Partially rescues

Scer\GAL4^elav.PLu, Vps35^EY14200 partially rescues Vps35^EY14200/Df(2R)ED3952

(Korolchuk et al., 2007)

Scer\GAL4^C57, Vps35^EY14200 partially rescues Vps35^EY14200/Df(2R)ED3952

(Korolchuk et al., 2007)

Comments

Expression of Vps35^EY14200 under the control of Scer\GAL4^Hml.PG rescues the endocytic defects seen in cultured Vps35^EY14200/Df(2R)ED3952 haemocytes in the absence of a Scer\GAL4 driver.

Expression of Vps35^EY14200 under the control of Scer\GAL4^Hml.PG rescues the increased number of haemocytes seen in Vps35^EY14200/Df(2R)ED3952 larvae in the absence of a Scer\GAL4 driver.

The increased bouton number at the neuromuscular junction that is seen in Vps35^EY14200/Df(2R)ED3952 larvae in the absence of a Scer\GAL4 driver is partially rescued by expression of Vps35^EY14200 under the control of one of Scer\GAL4^elav.PLu or Scer\GAL4^C57 and is fully rescued by expression of Vps35^EY14200 using both Scer\GAL4^elav.PLu and Scer\GAL4^C57 drivers simultaneously.