FlyBase Allele Report: Dmel\Mad[10]

General Information

Symbol

Dmel\Mad¹⁰

Species

D. melanogaster

Name

FlyBase ID

FBal0044914

Feature type

allele

Associated gene

Dmel\Mad

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

amorphic allele - genetic evidence

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

amorphic allele - genetic evidence

(Sekelsky et al., 1995)

Mutagen

ethyl methanesulfonate

(Sekelsky et al., 1995)

Progenitor genotype

Cytology

Description

Amino acid replacement: G409S.

(Hoodless et al., 1996, Sekelsky et al., 1995)

Nucleotide substitution: G1577A.

(Sekelsky et al., 1995)

Point mutation.

(Sekelsky et al., 1995)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

2L:3,147,562..3,147,562 [-]

Nucleotide change:

G3147562A

Reported nucleotide change:

G1577A

Amino acid change:

G409S | Mad-PA; G479S | Mad-PB

Reported amino acid change:

G409S

(Sekelsky et al., 1995)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal - all die before end of pupal stage | recessive

(Sekelsky et al., 1995)

some die during pupal stage | recessive

(Sekelsky et al., 1995)

Phenotype Manifest In

embryonic/first instar larval cuticle

(Das et al., 1998)

ventral denticle belt

(Das et al., 1998)

Detailed Description

Statement

Reference

Mad¹⁰ heterozygotes exhibit wild-type levels of synaptic boutons.

(Higashi-Kovtun et al., 2010)

Embryos lacking maternal and zygotic Mad function (Mad¹⁰/Mad¹² embryos derived from females with homozygous Mad¹² germ line clones) show loss of dorsal tissue and expansion of the lateral denticle bands into dorsal regions.

(Das et al., 1998)

Larval heterozygotes with Df(2L)JS17 exhibit reduced fat body, midgut defects and greatly reduced gastric caecae and dissected pupae exhibit absent or severely reduced imaginal discs.

(Sekelsky et al., 1995)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

Mad[+]/Mad¹⁰ is a suppressor of abnormal neuroanatomy | larval stage phenotype of Vps35^EY14200

(Korolchuk et al., 2007)

Mad[+]/Mad¹⁰ is a suppressor of visible phenotype of Scer\GAL4^en-e16E, sax^{Q263D.UAS.cDa}

(Das et al., 1998)

Mad[+]/Mad¹⁰ is a suppressor of visible phenotype of tkv^SC143

(Das et al., 1998)

Other

Statement

Reference

Impβ11⁷⁰, Mad¹⁰ has abnormal neuroanatomy phenotype

(Higashi-Kovtun et al., 2010)

Phenotype Manifest In

Enhancer of

Statement

Reference

Mad¹⁰ is an enhancer of phenotype of dpp^e87

(Sekelsky et al., 1995)

Mad¹⁰ is an enhancer of phenotype of dpp^hr56

(Sekelsky et al., 1995)

Suppressor of

Statement

Reference

Mad[+]/Mad¹⁰ is a suppressor of NMJ bouton phenotype of Vps35^EY14200

(Korolchuk et al., 2007)

Mad[+]/Mad¹⁰ is a suppressor of wing | posterior phenotype of Scer\GAL4^en-e16E, sax^{Q263D.UAS.cDa}

(Das et al., 1998)

Mad[+]/Mad¹⁰ is a suppressor of wing vein | ectopic phenotype of Scer\GAL4^en-e16E, sax^{Q263D.UAS.cDa}

(Das et al., 1998)

Mad[+]/Mad¹⁰ is a suppressor of wing phenotype of tkv^SC143

(Das et al., 1998)

Mad[+]/Mad¹⁰ is a suppressor of wing vein | ectopic phenotype of tkv^SC143

(Das et al., 1998)

Other

Statement

Reference

Impβ11⁷⁰, Mad¹⁰ has bouton phenotype

(Higashi-Kovtun et al., 2010)

Additional Comments

Genetic Interactions

Statement

Reference

Ranbp11⁷⁰/Mad¹⁰ double mutants exhibit a 15% reduction in synaptic bouton number.

(Higashi-Kovtun et al., 2010)

The increased bouton number at the neuromuscular junction that is seen in homozygous Vps35^EY14200 larvae is suppressed by Mad¹⁰/+.

(Korolchuk et al., 2007)

Df(1)su(s)R194/+ ; ex^NY1 Mad¹⁰ clones are recovered in adult eyes, although they are smaller than Df(1)su(s)R194/+ ; ex^NY1 clones.

(Tyler et al., 2007)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Comments

Mad mutations can be placed in an allelic series based on relative severity of the maternal effect enhancement of weak dpp alleles: Mad¹ < Mad⁷ < Mad¹² < Mad¹⁰ < Mad⁵ < Mad³ < Mad² < Mad¹¹ < Mad⁶ < Mad⁴ < Mad⁸ < Mad⁹.

(Sekelsky et al., 1995)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

MAD¹⁰

(Higashi-Kovtun et al., 2010)

Mad¹⁰

(Bivik et al., 2015, Oh and Irvine, 2011, Veverytsa and Allan, 2011, Ellis et al., 2010, Eivers et al., 2009, Miguel-Aliaga et al., 2008, Korolchuk et al., 2007, Tyler et al., 2007)

mad¹⁰

(Fregoso Lomas et al., 2016)

Name Synonyms

Secondary FlyBase IDs

References (15)

Report Sections

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