FlyBase Allele Report: Dmel\dco[EY02910]

General Information

Symbol

Dmel\dco^EY02910

Species

D. melanogaster

Name

FlyBase ID

FBal0159333

Feature type

allele

Associated gene

Dmel\dco

Associated Insertion(s)

P{EPgy2}dco^EY02910

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

hypomorphic allele - molecular evidence

Mutagen

P-element activity

Nature of the Allele

Allele class

hypomorphic allele - molecular evidence

(Zheng et al., 2014)

Mutagen

P-element activity

(Gene Disruption Project members, 2001-)

Progenitor genotype

Associated Insertion(s)

P{EPgy2}dco^EY02910

Cytology

Description

Insertion in a 5'UTR exon that is present in all dco isoforms.

(Zheng et al., 2014)

Allele components

Component

Use(s)

Inserted element

P{EPgy2}

misexpression element

Regulatory region(s)

UASp

binary expression system - regulatory region

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

3R:31,058,563..31,058,563 [-]

(Cziko et al., 2009, Gene Disruption Project members, 2001-)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal locomotor rhythm (with dco^ar)

(Zheng et al., 2014)

abnormal locomotor rhythm (with dco^L)

(Zheng et al., 2014)

abnormal locomotor rhythm (with dco^S)

(Zheng et al., 2014)

abnormal locomotor rhythm | recessive

(Zheng et al., 2014)

fertile

(Gene Disruption Project members, 2001-)

viable

(Gene Disruption Project members, 2001-)

Phenotype Manifest In

larval multidendritic class IV neuron | somatic clone, with Scer\GAL4^Act.PU

(Cziko et al., 2009)

Detailed Description

Statement

Reference

Under conditions of total darkness (DD), more than half of dco^EY02910/dco^EY02910 flies are arrhythmic, and most of the remainder show weak rhythms and a longer period. In DD, a small percentage of dco^EY02910/dco^L flies are arrhythmic and have a slightly longer period and dco^EY02910/dco^S flies have a slightly shorter period compared to wild type. In DD, almost half of dco^EY02910/dco^ar flies are arrhythmic, and most of the remainder show weak rhythms and a longer period.

(Zheng et al., 2014)

Over-expression of dco^EY02910 reduces dendrite branching, and alters dendritic morphology in larval class IV sensory neurons (induced under the control of Scer\GAL4^Act.PU). Total dendrite length is not significantly reduced in these neurons.

(Cziko et al., 2009)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

dco^EY02910 has abnormal locomotor rhythm phenotype, suppressible | partially by per^s

(Zheng et al., 2014)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Presence of per^s does not rescue arrhythmicity of dco^EY02910 flies but shortens the period of weakly rhythmic dco^EY02910 flies.

(Zheng et al., 2014)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

dco^EY02910 is rescued by Scer\GAL4^tim.PE, dco^EY02910

(Zheng et al., 2014)

Rescues

Scer\GAL4^tim.PE, dco^EY02910 rescues dco^EY02910

(Zheng et al., 2014)

Comments

Scer\GAL4^tim.PE driven expression of endogenous dco trapped by the EY insertion dco^EY02910 rescues defective locomotor rhythms in dco^EY02910/dco^EY02910 flies.

(Zheng et al., 2014)

Images (0)

Mutant

Wild-type

Stocks (4)

Bloomington

19662

y¹ w^67c23; P{EPgy2}dco^EY02910

80984

w¹¹¹⁸; tim^PL; P{EPgy2}dco^EY02910/TM6C, Sb¹

80982

w¹¹¹⁸; CkIIα^Tik P{EPgy2}dco^EY02910/TM6C, Sb¹