FlyBase Allele Report: Dmel\pain[EP2621]

General Information

Symbol

Dmel\pain^EP2621

Species

D. melanogaster

Name

FlyBase ID

FBal0146949

Feature type

allele

Associated gene

Dmel\pain

Associated Insertion(s)

P{EP}pain^EP2621

Carried in Construct

Also Known As

pain²

Key Links

Genomic Maps

JBrowse

Allele class

hypomorphic allele - molecular evidence

Mutagen

P-element activity

Nature of the Allele

Allele class

hypomorphic allele - molecular evidence

(Sakai et al., 2014)

Mutagen

P-element activity

(Tracey et al., 2003)

Progenitor genotype

Associated Insertion(s)

P{EP}pain^EP2621

Cytology

Description

pain^EP2621 contains P{EP}pain^EP2621 in the 5'UTR and contains a 12kb deficiency that deletes the promoter and enhancer elements of the pain gene.

(Al-Anzi et al., 2006)

Allele components

Component

Use(s)

Inserted element

P{EP}

misexpression element

Regulatory region(s)

UAS

binary expression system - regulatory region

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

2R:24,928,258..24,928,258 [+]

(Pradhan et al., 2024, Dhakal et al., 2022, Al-Anzi et al., 2006, Tracey et al., 2003)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal chemosensory behavior

(Al-Anzi et al., 2006)

abnormal memory

(Sakai et al., 2012)

abnormal memory (with pain^GAL4)

(Sakai et al., 2012)

abnormal pain response | semidominant

(Tracey et al., 2003)

abnormal taste perception

(Al-Anzi et al., 2006)

partially lethal - majority die

(Sakai et al., 2012)

short lived

(Sakai et al., 2012)

Phenotype Manifest In

embryonic/larval heart

(Sénatore et al., 2010)

Detailed Description

Statement

Reference

pain^EP2621 mutant larvae do not have reduced rolling (escape) behavior in response to noxious temperature increases, compared to controls.

(Luo et al., 2017)

pain^EP2621 mutant adults show normal preference for less solid sucrose-agarose food than a harder one (higher agarose conc., same sucrose content) one in a two-way food choice assay.

(Zhang et al., 2016)

pain^EP2621 mutant females show enhanced sexual receptivity. The mating success of homozygous pain^EP2621 mutant females is significantly higher than that of wild type females and the time required for copulation is significantly shorter. No significant difference is detected between wild type and pain^EP2621 females in the courtship latency (duration between introduction of flies and the first courtship), courtship index (percentage of time spent courting) or general locomotion.

The number of times pain^EP2621 females reject males is significantly lower than that of wild-type females.

(Sakai et al., 2014)

The gustatory aversion of mutant flies to 6mM camphor in a two way-choice test is not significantly different from that seen in wild-type flies.

(Zhang et al., 2013)

pain^EP2621 heterozygous adults are defective in courtship long term memory (reduction in courtship activity 5 days after 7-hours conditioning) while they exhibit normal courtship short term memory (reduction in courtship activity 30 minutes after 1-hour conditioning) when compared to controls.

(Sakai et al., 2012)

Mutant adults show normal avoidance of 1% citronellal in a direct airborne repellent test (DART) assay.

(Kwon et al., 2010)

Mutant third instar larvae show the same preference for 17.5[o]C over 14[o]C in a two-way choice test as do wild-type controls.

(Kwon et al., 2010)

Mutant larvae do not show the cardiac pauses during motion which are seen in wild-type larvae.

Basal cardiac activity (measured in anaesthetised animals) is increased in mutant larvae compared to wild-type controls.

Contractility of the heart tube is reduced in mutant larvae compared to wild-type controls.

(Sénatore et al., 2010)

pain^EP2621 mutants retain a preference for 18[o]C (i.e. are thermotactic).

(Kwon et al., 2008)

pain^EP2621 flies exhibit a reduced avoidance to isothiocyanate-containing food.

pain^EP2621 mutants exhibit normal avoidance to quinine and NaCl. They also exhibit wild-type levels of attraction to sucrose.

pain^EP2621 mutants exhibit wild-type levels of attraction to capsaicin.

pain^EP2451/pain^EP2621 trans-heterozygotes exhibit a reduced avoidance to isothiocyanate-containing food as in homozygous mutants.

(Al-Anzi et al., 2006)

Mutants are strongly insensitive to noxious heat.

(Tracey et al., 2003)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to complement

pain^EP2451

(Tracey et al., 2003)

pain^EP2462

(Tracey et al., 2003)

pain^EP2251

(Tracey et al., 2003)

Rescued by

pain^EP2621 is rescued by pain^+t8.5

(Sakai et al., 2012)

pain^EP2621 is rescued by pain^UAS.cTa/Scer\GAL4^pain-GAL4

(Al-Anzi et al., 2006)

Not rescued by

pain^EP2621 is not rescued by Scer\GAL4^Ilp2.PU/pain^UAS.cTa

(Sakai et al., 2014)

pain^EP2621 is not rescued by pain^GAL4

(Sakai et al., 2012)

Fails to rescue

pain^EP2621 fails to rescue pain^GAL4

(Sakai et al., 2012)

Comments

Expression of pain^Scer\UAS.cTa in insulin producing cells under the control of Scer\GAL4^Ilp2.PU is unable to rescue the increased sexual receptivity seen in pain^EP2621 mutant females.

(Sakai et al., 2014)

pain^EP2621/+ fails to rescue courtship long term memory phenotype of pain^GAL4/+.

Expression of pain^+t8.5 rescues while pain^GAL4/+ fails to rescue courtship long term memory phenotype of pain^EP2621/+ .

(Sakai et al., 2012)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

28799

w^*; P{EP}pain^EP2621/CyO; P{UAS-pain.T}AR9

Notes on Origin

Discoverer

Comments

Based on the avoidance to isothiocyanate-containing food in a two-choice preference test, the following alleles can be ranked from strongest response to weakest in the following order: pain^EP2451 > pain^EP2621 > pain^GAL4.

(Al-Anzi et al., 2006)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

pain2

(Sénatore et al., 2010)

pain²

(Pradhan et al., 2024, Dhakal et al., 2022, Rimal and Lee, 2019, Ohashi and Sakai, 2018, Luo et al., 2017, Zhang et al., 2016, Sakai et al., 2014, Zhang et al., 2013, Sakai et al., 2012, Kwon et al., 2010, Kwon et al., 2010, Kwon et al., 2008, Rosenzweig et al., 2008, Al-Anzi et al., 2006, Tracey et al., 2003)

pain^EP2621

Name Synonyms

Secondary FlyBase IDs

References (17)

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