FlyBase Allele Report: Dmel\park[UAS.cGa]

General Information

Symbol

Dmel\park^UAS.cGa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Greene

FlyBase ID

FBal0146935

Feature type

allele

Associated gene

Dmel\park

Associated Insertion(s)

Carried in Construct

P{UAS-park.G}

Also Known As

UAS-parkin, UAS-park

Key Links

Transgenic product class

cDNA

(Greene et al., 2003)

wild_type

(Greene et al., 2003)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Greene et al., 2003)

wild_type

(Greene et al., 2003)

Mutagen

in vitro construct

(Greene et al., 2003)

Progenitor genotype

Carried in construct

P{UAS-park.G}

Cytology

Description

UASt Sequences drive expression of a park cDNA.

(Greene et al., 2003)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

park

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of Parkinson's disease 2

CEA

(Martinez et al., 2017)

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates Parkinson's disease

modeled by Pink1^B9

(Pogson et al., 2014, Hao et al., 2010, Poole et al., 2008)

ameliorates late onset Parkinson's disease

modeled by Hsap\ZNF746^DM.UAS

(Pirooznia et al., 2020)

ameliorates amyotrophic lateral sclerosis type 10

modeled by Hsap\TARDBP^UAS.cDa

(Sun et al., 2018)

ameliorates Parkinson's disease 1

modeled by Hsap\SNCA^UAS.cFa

(Krzystek et al., 2021)

Comments on Models/Modifiers Based on Experimental Evidence ( 1 )

While expression of park^Scer\UAS.cGa ameliorates the behavioral phenotypes seen in Pink1^B9 mutants, it achieves this without restoring complex I activity.

(Pogson et al., 2014)

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal locomotor behavior | female limited, with Scer\GAL4^da.Switch.PT

(Rana et al., 2013)

abnormal locomotor behavior | female limited, with Scer\GAL4^{elav.Switch.PO}

(Rana et al., 2013)

abnormal starvation stress response, with Scer\GAL4^da.Switch.PT

(Rana et al., 2013)

abnormal starvation stress response, with Scer\GAL4^{elav.Switch.PO}

(Rana et al., 2013)

decreased cell number | adult stage | progressive, with Scer\GAL4^ple.PF

(Pirooznia et al., 2020)

increased fecundity | RU486 conditional, with Scer\GAL4^da.Switch.PT

(Rana et al., 2013)

long lived | female limited, with Scer\GAL4^da.Switch.PT

(Rana et al., 2013)

long lived | female limited, with Scer\GAL4^{elav.Switch.PO}

(Rana et al., 2013)

partially lethal - majority die, with Scer\GAL4^Act5C.PI

(Pirooznia et al., 2020)

viable, with Scer\GAL4^Act5C.PI

(Pirooznia et al., 2020)

viable, with Scer\GAL4^da.PU

(Hao et al., 2010)

Phenotype Manifest In

mitochondrion | female limited, with Scer\GAL4^da.Switch.PT

(Rana et al., 2013)

mitochondrion | female limited, with Scer\GAL4^{elav.Switch.PO}

(Rana et al., 2013)

Detailed Description

Statement

Reference

Ubiquitous expression of park^Scer\UAS.cGa throughout development and adulthood under the control of Scer\GAL4^da.Switch.PT (and treatment with RU486) results in an increase in the mean and maximum lifespans of female flies. Smaller effects are also seen in male flies. These long lived flies display normal food consumption and an increase in spontaneous physical activity in young flies. Young flies also show an increase in fecundity. Hypoxia resistance is similar to controls, but improved survival is seen following starvation. Expressing park^Scer\UAS.cGa only during development has no major impact on adult longevity, whereas Scer\GAL4^da.Switch.PT>park^Scer\UAS.cGa flies are significantly longer lived when exposed to RU486 exclusively in the adult stage.

Ubiquitous expression of park^Scer\UAS.cGa under the control of Scer\GAL4^da.Switch.PT results in reduced levels of protein aggregates during ageing compared to controls. The average size of aggregates is also significantly reduced.

The mitochondria of young and aged flies expressing park^Scer\UAS.cGa under the control of Scer\GAL4^da.Switch.PT are fragmented (smaller and rounder) compared to controls. There is an increase in citrate synthase and mitochondrial respiratory complexes I and II activities in young flies, and an increase in citrate synthase activity in aged flies.

Constitutive expression of park^Scer\UAS.cGa in neurons under the control of Scer\GAL4^{elav.Switch.PO} (and treatment with RU486) results in an increase in the mean and maximum lifespans of female flies. An increase in lifespan is also seen when expression is limited only to the adult stages. No major effect is seen in male flies. Neuronal expression of park^Scer\UAS.cGa does not reduce food consumption or increase physical activity or fecundity in young flies. Improved survival is seen following starvation.

Neuronal expression of park^Scer\UAS.cGa under the control of Scer\GAL4^{elav.Switch.PO} results in reduced levels of protein aggregates during ageing compared to controls.

The mitochondria of young and aged flies expressing park^Scer\UAS.cGa under the control of Scer\GAL4^da.Switch.PT show an increase in citrate synthase in young flies and mitochondrial respiratory complex I activity in aged flies.

(Rana et al., 2013)

Expression of park^Scer\UAS.cGa under the control of Scer\GAL4^GMR.PF does not have any detectable effect on eye morphology.

(Poole et al., 2008)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT suppressed by

Statement

Reference

Pink1^UAS.Tag:HA, Scer\GAL4^GMR.PF, park^UAS.cGa has visible | adult stage phenotype, non-suppressible by HtrA2^Δ1

(Whitworth et al., 2008)

Scer\GAL4^GMR.PF, park^UAS.cGa, rho-7^UAS.cWa has visible phenotype, non-suppressible by HtrA2^Δ1

(Whitworth et al., 2008)

Enhancer of

Statement

Reference

park^UAS.cGa, Scer\GAL4^GMR.PF is an enhancer of visible phenotype of Pink1^UAS.Tag:HA, Scer\GAL4^GMR.PF

(Poole et al., 2008)

park^UAS.cGa, Scer\GAL4^GMR.PF is an enhancer of visible | adult stage phenotype of Pink1^UAS.Tag:HA, Scer\GAL4^GMR.PF

(Whitworth et al., 2008)

park^UAS.cGa/Scer\GAL4^GMR.PF is an enhancer of visible phenotype of Scer\GAL4^GMR.PF, rho-7^UAS.cWa

(Whitworth et al., 2008)

Suppressor of

Statement

Reference

Scer\GAL4^Act5C.PI/park^UAS.cGa is a suppressor of decreased rate of adult locomotory behavior phenotype of Scer\GAL4^Act5C.PI, Usp30^UAS.cUa

(Sanchez-Martinez et al., 2023)

park^UAS.cGa, Scer\GAL4^Appl.G1a is a suppressor of increased cell death | larval stage phenotype of Hsap\SNCA^UAS.cFa, Scer\GAL4^Appl.G1a

(Krzystek et al., 2021)

park^UAS.cGa, Scer\GAL4^ple.PF is a suppressor of abnormal neuroanatomy | adult stage | progressive phenotype of Paris^{UAS.ORF.GW.Tag:HA}, Scer\GAL4^ple.PF

(Pirooznia et al., 2020)

park^UAS.cGa, Scer\GAL4^ple.PF is a suppressor of decreased cell number | adult stage | progressive phenotype of Paris^{UAS.ORF.GW.Tag:HA}, Scer\GAL4^ple.PF

(Pirooznia et al., 2020)

park^UAS.cGa, Scer\GAL4^ple.PF is a suppressor of abnormal locomotor behavior | adult stage | progressive phenotype of Paris^{UAS.ORF.GW.Tag:HA}, Scer\GAL4^ple.PF

(Pirooznia et al., 2020)

park^UAS.cGa, Scer\GAL4^Act5C.PI is a suppressor | partially of increased mortality during development phenotype of Hsap\ZNF746^UAS.cDa, Scer\GAL4^Act5C.PI

(Pirooznia et al., 2020)

park^UAS.cGa, Scer\GAL4^ple.PF is a suppressor of abnormal neuroanatomy | adult stage | progressive phenotype of Hsap\ZNF746^UAS.cDa, Scer\GAL4^ple.PF

(Pirooznia et al., 2020)

park^UAS.cGa, Scer\GAL4^ple.PF is a suppressor of abnormal locomotor behavior | adult stage | progressive phenotype of Hsap\ZNF746^UAS.cDa, Scer\GAL4^ple.PF

(Pirooznia et al., 2020)

Scer\GAL4^da.G32/park^UAS.cGa is a suppressor | partially of lethal - all die before end of pupal stage | recessive phenotype of ari-1^A

(Tan et al., 2018)

Scer\GAL4^da.G32/park^UAS.cGa is a suppressor | partially of short lived phenotype of ari-1^T-GT15

(Tan et al., 2018)

park^UAS.cGa/Scer\GAL4^GMR.PF is a suppressor of visible phenotype of Scer\GAL4^GMR.PF, cindr^{RNAi.PC.PD.UAS}

(Ketosugbo et al., 2017)

Scer\GAL4^ple.PU/park^UAS.cGa is a suppressor of abnormal locomotor behavior | adult stage phenotype of Scer\GAL4^ple.PU, Stoml2^GD16706

(Zanon et al., 2017)

park^UAS.cGa, Scer\GAL4^ple.PU is a suppressor of abnormal locomotor behavior | adult stage phenotype of Df(2R)BSC695, Scer\GAL4^ple.PU, Stoml2^GD16706

(Zanon et al., 2017)

park^UAS.cGa, Scer\GAL4^ple.PU is a suppressor of abnormal locomotor behavior | adult stage phenotype of Df(2R)BSC770, Scer\GAL4^ple.PU, Stoml2^GD16706

(Zanon et al., 2017)

Scer\GAL4^fln.IFM/park^UAS.cGa is a suppressor of visible phenotype of Scer\GAL4^fln.IFM, Stoml2^GD16706

(Zanon et al., 2017)

park^UAS.cGa, Scer\GAL4^fln.IFM is a suppressor of visible phenotype of Df(2R)BSC695, Scer\GAL4^fln.IFM, Stoml2^GD16706

(Zanon et al., 2017)

park^UAS.cGa, Scer\GAL4^fln.IFM is a suppressor of visible phenotype of Df(2R)BSC770, Scer\GAL4^fln.IFM, Stoml2^GD16706

(Zanon et al., 2017)

Scer\GAL4^Mef2.PR/park^UAS.cGa is a suppressor of abnormal locomotor behavior | third instar larval stage phenotype of Scer\GAL4^Mef2.PR, clu^GD13926

(Wang et al., 2016)

Scer\GAL4^Mef2.PR/park^UAS.cGa is a suppressor of partially lethal - majority die phenotype of Scer\GAL4^Mef2.PR, clu^GD13926

(Wang et al., 2016)

Scer\GAL4^da.PU/park^UAS.cGa is a suppressor of abnormal flight phenotype of Pink1^B9

(Pogson et al., 2014)

Scer\GAL4^da.PU/park^UAS.cGa is a suppressor of abnormal locomotor behavior | adult stage phenotype of Pink1^B9

(Pogson et al., 2014)

Scer\GAL4^da.G32/park^UAS.cGa is a suppressor | partially of visible phenotype of Pink1^B9

(de Castro et al., 2013)

park^UAS.cGa, Scer\GAL4^da.G32 is a suppressor of abnormal locomotor behavior | adult stage | progressive phenotype of Rnor\Otc^d.UAS, Scer\GAL4^da.G32

(Pimenta de Castro et al., 2012)

Scer\GAL4^da.PU/park^UAS.cGa is a suppressor of visible phenotype of Pink1^B9

(Hao et al., 2010)

Scer\GAL4^da.PU/park^UAS.cGa is a suppressor of increased cell death | adult stage phenotype of Pink1^B9

(Hao et al., 2010)

Scer\GAL4^Mef2.PR/park^UAS.cGa is a suppressor of abnormal flight phenotype of Pink1^B9

(Poole et al., 2008)

Scer\GAL4^Mef2.PR/park^UAS.cGa is a suppressor of visible phenotype of Pink1^B9

(Poole et al., 2008)

NOT Suppressor of

Statement

Reference

park^UAS.cGa, Scer\GAL4^ple.PF is a non-suppressor of abnormal neuroanatomy | adult stage phenotype of Hsap\ZNF746^DM.UAS, Scer\GAL4^ple.PF

(Pirooznia et al., 2020)

park^UAS.cGa, Scer\GAL4^ple.PF is a non-suppressor of decreased cell number | adult stage phenotype of Hsap\ZNF746^DM.UAS, Scer\GAL4^ple.PF

(Pirooznia et al., 2020)

Scer\GAL4^da.G32/park^UAS.cGa is a non-suppressor of partially lethal | recessive phenotype of ari-1^T-GT15

(Tan et al., 2018)

Scer\GAL4^da.PU/park^UAS.cGa is a non-suppressor of abnormal locomotor behavior phenotype of Pink1^B9

(Tsai et al., 2018)

Scer\GAL4^ey-OK107/park^UAS.cGa is a non-suppressor of abnormal neuroanatomy | somatic clone | adult stage | progressive phenotype of bsk^flp147E

(Rallis et al., 2013)

Other

Statement

Reference

Scer\GAL4^da.G32, ari-1^A, park^UAS.cGa has short lived phenotype

(Tan et al., 2018)

DJ-1α^Δ72, Scer\GAL4^da.PU, dj-1β^Δ93, park^UAS.cGa has lethal phenotype

(Hao et al., 2010)

Phenotype Manifest In

NOT suppressed by

Statement

Reference

Pink1^UAS.Tag:HA, Scer\GAL4^GMR.PF, park^UAS.cGa has eye | adult stage phenotype, non-suppressible by HtrA2^Δ1

(Whitworth et al., 2008)

Pink1^UAS.Tag:HA, Scer\GAL4^GMR.PF, park^UAS.cGa has ommatidium | adult stage phenotype, non-suppressible by HtrA2^Δ1

(Whitworth et al., 2008)

Scer\GAL4^GMR.PF, park^UAS.cGa, rho-7^UAS.cWa has eye phenotype, non-suppressible by HtrA2^Δ1

(Whitworth et al., 2008)

Scer\GAL4^GMR.PF, park^UAS.cGa, rho-7^UAS.cWa has interommatidial bristle phenotype, non-suppressible by HtrA2^Δ1

(Whitworth et al., 2008)

Enhancer of

Statement

Reference

park^UAS.cGa, Scer\GAL4^GMR.PF is an enhancer of eye phenotype of Pink1^UAS.Tag:HA, Scer\GAL4^GMR.PF

(Poole et al., 2008)

park^UAS.cGa, Scer\GAL4^GMR.PF is an enhancer of eye | adult stage phenotype of Pink1^UAS.Tag:HA, Scer\GAL4^GMR.PF

(Whitworth et al., 2008)

park^UAS.cGa, Scer\GAL4^GMR.PF is an enhancer of ommatidium | adult stage phenotype of Pink1^UAS.Tag:HA, Scer\GAL4^GMR.PF

(Whitworth et al., 2008)

park^UAS.cGa/Scer\GAL4^GMR.PF is an enhancer of eye phenotype of Scer\GAL4^GMR.PF, rho-7^UAS.cWa

(Whitworth et al., 2008)

park^UAS.cGa/Scer\GAL4^GMR.PF is an enhancer of interommatidial bristle phenotype of Scer\GAL4^GMR.PF, rho-7^UAS.cWa

(Whitworth et al., 2008)

Suppressor of

Statement

Reference

park^UAS.cGa, Scer\GAL4^Appl.G1a is a suppressor of embryonic/larval brain | larval stage phenotype of Hsap\SNCA^UAS.cFa, Scer\GAL4^Appl.G1a

(Krzystek et al., 2021)

park^UAS.cGa, Scer\GAL4^ple.PF is a suppressor of dopaminergic neuron | adult stage | decreased number phenotype of Paris^{UAS.ORF.GW.Tag:HA}, Scer\GAL4^ple.PF

(Pirooznia et al., 2020)

park^UAS.cGa, Scer\GAL4^ple.PF is a suppressor of dopaminergic neuron | adult stage | decreased number phenotype of Hsap\ZNF746^UAS.cDa, Scer\GAL4^ple.PF

(Pirooznia et al., 2020)

park^UAS.cGa, Scer\GAL4^ple.PF is a suppressor of mitochondrion | adult stage phenotype of Hsap\ZNF746^UAS.cDa, Scer\GAL4^ple.PF

(Pirooznia et al., 2020)

Scer\GAL4^da.G32/park^UAS.cGa is a suppressor of embryonic/larval hypodermal muscle cell | third instar larval stage phenotype of ari-1^A

(Tan et al., 2018)

Scer\GAL4^da.G32/park^UAS.cGa is a suppressor of nucleus | third instar larval stage phenotype of ari-1^A

(Tan et al., 2018)

park^UAS.cGa/Scer\GAL4^GMR.PF is a suppressor of eye phenotype of Scer\GAL4^GMR.PF, cindr^{RNAi.PC.PD.UAS}

(Ketosugbo et al., 2017)

park^UAS.cGa/Scer\GAL4^GMR.PF is a suppressor of retina | P-stage phenotype of Scer\GAL4^GMR.PF, cindr^{RNAi.PC.PD.UAS}

(Ketosugbo et al., 2017)

park^UAS.cGa/Scer\GAL4^GMR.PF is a suppressor of interommatidial cell | P-stage phenotype of Scer\GAL4^GMR.PF, cindr^{RNAi.PC.PD.UAS}

(Ketosugbo et al., 2017)

Scer\GAL4^fln.IFM/park^UAS.cGa is a suppressor of wing phenotype of Scer\GAL4^fln.IFM, Stoml2^GD16706

(Zanon et al., 2017)

park^UAS.cGa, Scer\GAL4^fln.IFM is a suppressor of wing phenotype of Df(2R)BSC695, Scer\GAL4^fln.IFM, Stoml2^GD16706

(Zanon et al., 2017)

park^UAS.cGa, Scer\GAL4^fln.IFM is a suppressor of wing phenotype of Df(2R)BSC770, Scer\GAL4^fln.IFM, Stoml2^GD16706

(Zanon et al., 2017)

Scer\GAL4^Mef2.PR/park^UAS.cGa is a suppressor of mitochondrion | adult stage phenotype of Scer\GAL4^Mef2.PR, clu^GD13926

(Wang et al., 2016)

Scer\GAL4^how-24B/park^UAS.cGa is a suppressor of mitochondrion | third instar larval stage phenotype of Pink1^B9

(Zhu et al., 2015)

Scer\GAL4^how-24B/park^UAS.cGa is a suppressor of embryonic/larval somatic muscle cell | third instar larval stage phenotype of Pink1^B9

(Zhu et al., 2015)

Scer\GAL4^da.G32/park^UAS.cGa is a suppressor | partially of adult thorax phenotype of Pink1^B9

(de Castro et al., 2013)

park^UAS.cGa, Scer\GAL4^da.G32 is a suppressor of mitochondrion phenotype of Rnor\Otc^d.UAS, Scer\GAL4^da.G32

(Pimenta de Castro et al., 2012)

park^UAS.cGa, Scer\GAL4^da.G32 is a suppressor of indirect flight muscle cell phenotype of Rnor\Otc^d.UAS, Scer\GAL4^da.G32

(Pimenta de Castro et al., 2012)

Scer\GAL4^da.PU/park^UAS.cGa is a suppressor of wing phenotype of Pink1^B9

(Hao et al., 2010)

Scer\GAL4^da.PU/park^UAS.cGa is a suppressor of adult somatic muscle cell of thorax phenotype of Pink1^B9

(Hao et al., 2010)

Scer\GAL4^Mef2.PR/park^UAS.cGa is a suppressor | partially of mitochondrion phenotype of Pink1⁵

(Deng et al., 2008)

Scer\GAL4^Mef2.PR/park^UAS.cGa is a suppressor of adult thorax phenotype of Pink1^B9

(Poole et al., 2008)

Scer\GAL4^how-24B/park^UAS.cGa is a suppressor | partially of mitochondrial crista & indirect flight muscle phenotype of Pink1⁵

(Clark et al., 2006)

NOT Suppressor of

Statement

Reference

park^UAS.cGa, Scer\GAL4^Appl.G1a is a non-suppressor of mitochondrion | larval stage phenotype of Hsap\SNCA^1-120.UAS, Scer\GAL4^Appl.G1a

(Krzystek et al., 2021)

park^UAS.cGa, Scer\GAL4^ple.PF is a non-suppressor of dopaminergic neuron | adult stage | decreased number phenotype of Hsap\ZNF746^DM.UAS, Scer\GAL4^ple.PF

(Pirooznia et al., 2020)

Scer\GAL4^da.PU/park^UAS.cGa is a non-suppressor of mitochondrion | third instar larval stage phenotype of Pink1⁵

(Tsai et al., 2018)

Scer\GAL4^da.PU/park^UAS.cGa is a non-suppressor of mitochondrial crista | third instar larval stage phenotype of Pink1⁵

(Tsai et al., 2018)

Scer\GAL4^da.PU/park^UAS.cGa is a non-suppressor of embryonic/larval hypodermal muscle cell | third instar larval stage phenotype of Pink1⁵

(Tsai et al., 2018)

Scer\GAL4^ey-OK107/park^UAS.cGa is a non-suppressor of alpha/beta Kenyon cell phenotype of bsk^flp147E

(Rallis et al., 2013)

Scer\GAL4^ey-OK107/park^UAS.cGa is a non-suppressor of gamma Kenyon cell phenotype of bsk^flp147E

(Rallis et al., 2013)

Additional Comments

Genetic Interactions

Statement

Reference

The wing posture defects induced by the expression of Stoml2^GD16706 under the control of Scer\GAL4^fln.IFM alone, or in combination with heterozygosity for either Df(2R)BSC695 or Df(2R)BSC770, are fully suppressed by the co-expression of park^UAS.cGa.

The decreased adult climbing capacity observed upon the expression of Stoml2^GD16706 under the control of Scer\GAL4^ple.PU alone, or in combination with heterozygosity for either Df(2R)BSC695 or Df(2R)BSC770, is fully suppressed by the co-expression of park^UAS.cGa.

(Zanon et al., 2017)

The larval locomotor deficit, very low adult eclosion rate as well as the mitochondrial morphology (enlarged clustered mitochondria) and functional (loss of inner mitochondrial potential measured using cationic fluorescent dye JC-1) defects in adult indirect flight muscles characteristic for animals expressing clu^GD13926 under the control of Scer\GAL4^Mef2.PR can be suppressed by co-expression of park^Scer\UAS.cGa.

(Wang et al., 2016)

The enlarged or aggregated mitochondria phenotype observed in the body wall muscles of Pink1^B9 hemizygous male third instar larvae is suppressed either by combination with mask^10.22/mask^EY13048 alleles or by ectopic expression of park^Scer\UAS.cGa under the control of Scer\GAL4^how-24B.

(Zhu et al., 2015)

Ubiquitous expression of park^Scer\UAS.cGa under the control of Scer\GAL4^da.PU restores climbing and flight ability in Pink1^B9 mutant flies.

Expression of park^Scer\UAS.cGa under the control of Scer\GAL4^da.PU rescues the reduction in ATP level seen in Pink1^B9 mutant flies, but complex I activity is not significantly increased.

(Pogson et al., 2014)

Expression of park^Scer\UAS.cGa under the control of Scer\GAL4^ey-OK107 fails to suppress the axon degeneration seen in bsk^flp147E mutant mushroom body neuron clones.

(Rallis et al., 2013)

Expression of park^Scer\UAS.cGa under the control of Scer\GAL4^da.G32 significantly suppresses the penetrance of the indented thorax phenotype seen in Pink1^B9 mutants. This suppression is reduced if the flies are also carrying either ref(2)P^od2 or ref(2)P^od3.

The ability of park^Scer\UAS.cGa expressed under the control of Scer\GAL4^da.G32 to suppress the Pink1^B9 mutant phenotypes of an indented thorax and reduced climbing ability is suppressed if the flies are also mutant for Atg1^Δ3D.

(de Castro et al., 2013)

Expression of park^Scer\UAS.cGa under the control of Scer\GAL4^da.PU results in lethality in the DJ-1α^Δ72/DJ-1α^Δ72, dj-1β^Δ93/dj-1β^Δ93 double mutant background.

Expression of park^Scer\UAS.cGa under the control of Scer\GAL4^da.PU rescues the wing posture defect and thoracic muscle apoptosis phenotype of Pink1^B9/Pink1^B9 mutants.

(Hao et al., 2010)

Expression of park^Scer\UAS.cGa under the control of Scer\GAL4^Mef2.PR partially rescues the defects in mitochondrial morphology that are seen in the indirect flight muscles of Pink1⁵ mutants.

(Deng et al., 2008)

Expression of park^Scer\UAS.cGa under the control of Scer\GAL4^Mef2.PR fully rescues the thoracic indentation and flight phenotypes of Pink1^B9 mutants.

The rough eye phenotype of flies expressing Pink1^{Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^GMR.PF is enhanced by co-expression of park^Scer\UAS.cGa.

(Poole et al., 2008)

Co-expression of Pink1^{Scer\UAS.T:Ivir\HA1} and park^Scer\UAS.cGa under the control of Scer\GAL4^GMR.PF results in a severe rough eye phenotype, which greatly exceeds that conferred when Pink1^{Scer\UAS.T:Ivir\HA1} is expressed alone. This phenotype is not suppressed in a HtrA2^Δ1 mutant background.

No eye phenotypes are observed when park^Scer\UAS.cGa and HtrA2^Scer\UAS.cPa are co-expressed under the control of Scer\GAL4^GMR.PF.

Expression of park^Scer\UAS.cGa enhances the rough eye phenotype seen when rho-7^Scer\UAS.cWa is expressed under the control of Scer\GAL4^GMR.PF, resulting in severe loss of eye tissue. This phenotype is not suppressed by HtrA2^Δ1.

(Whitworth et al., 2008)

The indirect flight muscles of 2 day old Pink1⁵ adults that are also expressing park^Scer\UAS.cGa under the control of Scer\GAL4^how-24B have some mitochondria with normal densely packed cristae and some with fragmented cristae, in contrast to 2 day old Pink1⁵ single mutants, where the mitochondria of the indirect flight muscles have fragmented cristae.

(Clark et al., 2006)

Xenogenetic Interactions

Statement

Reference

Co-expression of park^Scer\UAS.cGa rescues the age-dependent defect in climbing ability seen in flies expressing Rnor\Otc^d.Scer\UAS under the control of Scer\GAL4^da.G32. The defects in indirect flight muscle structure and mitochondrial integrity are also suppressed.

Co-expression of park^Scer\UAS.cGa is no longer able to suppress the defects in climbing ability and indirect flight muscle structure seen in flies expressing Rnor\Otc^d.Scer\UAS under the control of Scer\GAL4^da.G32 when the flies are also mutant for Atg1^Δ3D.

(Pimenta de Castro et al., 2012)

Complementation and Rescue Data

Rescues

park^UAS.cGa rescues park^Δ21/park²⁵

(Zhu et al., 2015)

Scer\GAL4^Act5C.PU/park^UAS.cGa rescues park^Z3678/park²⁵

(Vincent et al., 2012)

Scer\GAL4^how-24B/park^UAS.cGa rescues Df(3L)Pc-MK/park²⁵

(Greene et al., 2003)

Scer\GAL4^how-24B/park^UAS.cGa rescues Df(3L)Pc-MK/park¹³

(Greene et al., 2003)

Scer\GAL4^Mef2.PR/park^UAS.cGa rescues Df(3L)Pc-MK/park¹³

(Greene et al., 2003)

Partially rescues

park^UAS.cGa/Scer\GAL4^elav.PLu partially rescues park^Z3678/park²⁵

(Vincent et al., 2012)

Scer\GAL4^G14/park^UAS.cGa partially rescues park^Z3678/park²⁵

(Vincent et al., 2012)

Scer\GAL4^Act5C.PU/park^UAS.cGa partially rescues park²⁵

(Vincent et al., 2012)

park^UAS.cGa/Scer\GAL4^elav.PLu partially rescues park²⁵

(Vincent et al., 2012)

Scer\GAL4^G14/park^UAS.cGa partially rescues park²⁵

(Vincent et al., 2012)

Scer\GAL4^ple.PF/park^UAS.cGa partially rescues park²⁵

(Whitworth et al., 2005)

Comments

Expression of park^Scer\UAS.cGa under the control of the Scer\GAL4^how-24B driver rescues the defective mitochondria observed in body wall muscles of park²⁵/park^Δ21 third instar larvae.

(Zhu et al., 2015)

Expression of park^Scer\UAS.cGa with a global (Scer\GAL4^Act5C.PU) or neuronal (Scer\GAL4^elav.PLu), but not muscle (Scer\GAL4^G14), driver rescues locomotion in park²⁵/park^Z3678 larvae; global and neuronal (but not muscle) expression partially rescues locomotion in park²⁵/park²⁵ larvae; neuronal but not muscle driver rescues contraction frequency in park²⁵/park²⁵ larvae; muscle expression rescues muscle resting membrane potential but not synaptic potential in park²⁵/park^Z3678 larvae; neuronal expression partially rescues synaptic potential but not muscle resting membrane potential in park²⁵/park^Z3678 larvae; global, neuronal and muscle drivers rescue synaptic overgrowth in park²⁵/park²⁵ larvae.

(Vincent et al., 2012)

Expression of park^Scer\UAS.cGa in the PPL neurons, under the regulation of Scer\GAL4^ple.PF significantly attenuates dopaminergic (DA) neuron loss in the PPL cluster in park²⁵ mutants.

(Whitworth et al., 2005)

The addition of park^Scer\UAS.cGa driven by Scer\GAL4^how-24B or Scer\GAL4^Mef2.PR rescues the flight and climbing behaviour defects seen in park¹³/Df(3L)Pc-MK animals. park^Scer\UAS.cGa driven by Scer\GAL4^how-24B rescues the mitochondrial and myofibril defects seen in park²⁵/Df(3L)Pc-MK indirect flight muscles.

(Greene et al., 2003)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

51651

w^*; P{UAS-park.G}L2/CyO

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

park^Scer\UAS.cGa

park^UAS.cGa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Greene

(Greene et al., 2003)

Secondary FlyBase IDs

References (36)

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