FlyBase Allele Report: Hsap\APP[695.UAS.Tag:MYC]

General Information

Symbol

Hsap\APP^{695.UAS.Tag:MYC}

Species

H. sapiens

Name

FlyBase ID

FBal0138483

Feature type

allele

Associated gene

Hsap\APP

Associated Insertion(s)

Carried in Construct

P{UAS-APP695-N-myc}

Also Known As

UAS-APP, UAS-APP695-N-myc, UAS-hAPP, UAS-APP⁶⁹⁵

Key Links

Transgenic product class

wild_type

(Merdes, 2002.9)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Merdes, 2002.9)

Mutagen

in vitro construct

(Merdes, 2002.9)

Progenitor genotype

Carried in construct

P{UAS-APP695-N-myc}

Cytology

Description

Similar to P{UAS-APP.695}, though tagged with Tag:MYC at the N-terminal end.

(Merdes, 2002.9)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

Hsap\APP

Tagged with

Tag:MYC

epitope tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of Alzheimer's disease

CEA

(Panikker et al., 2018, Xu et al., 2016, Shaw et al., 2015, Xu et al., 2014, Shaw and Chang, 2013, Pirooznia et al., 2012, Pirooznia et al., 2012)

CEA with Hsap\BACE1^UAS.Exel

(Adedayo et al., 2023, Chocron et al., 2022, Ogunsuyi et al., 2022, Wang et al., 2022, Rimal et al., 2021, Tsakiri et al., 2021, Mhatre et al., 2014)

CEA with Hsap\BACE1^UAS.cUa

(Zhu et al., 2022)

model of Alzheimer's disease 1

CEA

(Armour et al., 2023, Shao et al., 2022)

CEC

(Wang et al., 2022)

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

model of Alzheimer's disease

is exacerbated by sra^JF02557

(Shaw and Chang, 2013)

is exacerbated by Pp2B-14D^Act.Δ.UAS

(Shaw and Chang, 2013)

is ameliorated by CanB^JF02616

(Shaw et al., 2015, Shaw and Chang, 2013)

is ameliorated by sgg¹

(Shaw and Chang, 2013)

is exacerbated by sra¹

(Shaw and Chang, 2013)

is ameliorated by sra^UAS.Tag:HA

(Shaw et al., 2015, Shaw and Chang, 2013)

is ameliorated by Tip60^UAS.cLa

(Xu et al., 2016, Xu et al., 2014, Johnson et al., 2013, Pirooznia et al., 2012)

is ameliorated by Tip60^UAS.cPa

(Panikker et al., 2018, Pirooznia et al., 2012)

is ameliorated by Pka-C1^UAS.Tag:FLAG

(Shaw et al., 2015)

is exacerbated by Tip60^E431Q.UAS

(Xu et al., 2016, Johnson et al., 2013)

model of Alzheimer's disease 1

is ameliorated by CanB^JF02616

(Shao et al., 2022)

is ameliorated by Itpr^JF01957

(Shao et al., 2022)

is ameliorated by Mmmm\PV^UAS.Tag:MYC

(Shao et al., 2022)

is ameliorated by Ca-α1D^HMS00294

(Shao et al., 2022)

is ameliorated by NFAT^Δa1

(Shao et al., 2022)

is ameliorated by NFAT^Δab

(Shao et al., 2022)

is ameliorated by NFAT^Δb

(Shao et al., 2022)

is ameliorated by SARS-CoV-2\nsp1^UAS.cZa

(Wang et al., 2022)

is ameliorated by Adk2^GD6724

(Huichalaf et al., 2019)

is ameliorated by Pdk1^GD7480

(Huichalaf et al., 2019)

is ameliorated by inaC^KK105562

(Huichalaf et al., 2019)

is ameliorated by rl^GD12910

(Huichalaf et al., 2019)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal courtship behavior, with Scer\GAL4^Gr33a-GAL4

(Hu et al., 2015)

abnormal learning | adult stage, with Scer\GAL4^ey-OK107

(Xu et al., 2014)

abnormal learning | third instar larval stage, with Scer\GAL4^elav-C155

(Panikker et al., 2018)

abnormal locomotor behavior | third instar larval stage, with Scer\GAL4^elav.PU

(Shaw and Chang, 2013)

abnormal locomotor behavior | third instar larval stage, with Scer\GAL4^elav-C155

(Johnson et al., 2013)

abnormal memory | adult stage, with Scer\GAL4^c739

(Shaw et al., 2015)

abnormal memory | adult stage, with Scer\GAL4^ey-OK107

(Xu et al., 2014)

abnormal memory | adult stage | RU486 conditional, with Scer\GAL4^{Mef2.247.Switch}

(Shaw et al., 2015)

abnormal memory | third instar larval stage, with Scer\GAL4^elav-C155

(Panikker et al., 2018)

abnormal neuroanatomy, with Scer\GAL4^Gr33a-GAL4

(Hu et al., 2015)

abnormal neuroanatomy | adult stage, with Scer\GAL4^ey-OK107

(Xu et al., 2016)

abnormal neuroanatomy | adult stage | progressive, with Scer\GAL4^GMR.PU

(Shaw and Chang, 2013)

abnormal neuroanatomy | larval stage, with Scer\GAL4^ppk.PU

(Pizzano et al., 2023)

abnormal neuroanatomy | third instar larval stage, with Scer\GAL4^elav.PU

(Shaw and Chang, 2013)

abnormal phototaxis | adult stage | progressive, with Scer\GAL4^GMR.PU

(Shaw and Chang, 2013)

abnormal size | third instar larval stage, with Scer\GAL4^ey-OK107

(Panikker et al., 2018)

abnormal sleep | adult stage, with Scer\GAL4^P2.4.Pdf

(Pirooznia et al., 2012)

decreased cell number, with Scer\GAL4^Gr33a-GAL4

(Hu et al., 2015)

increased cell death | third instar larval stage, with Scer\GAL4^179Y

(Pirooznia et al., 2012)

majority die during P-stage, with Scer\GAL4^179Y

(Pirooznia et al., 2012)

majority die during P-stage, with Scer\GAL4^337Y

(Pirooznia et al., 2012)

partially lethal - majority die, with Scer\GAL4^337Y

(Pirooznia et al., 2012)

partially lethal - majority die, with Scer\GAL4^elav.PU

(Lee et al., 2016)

short lived, with Scer\GAL4^337Y

(Pirooznia et al., 2012)

visible | adult stage, with Scer\GAL4^GMR.PU

(Lee et al., 2016)

Phenotype Manifest In

adult mushroom body, with Scer\GAL4^ey-OK107

(Xu et al., 2016)

axon | adult stage | progressive, with Scer\GAL4^GMR.PU

(Shaw and Chang, 2013)

axon | larval stage, with Scer\GAL4^ppk.PU

(Pizzano et al., 2023)

axon | third instar larval stage, with Scer\GAL4^elav.PU

(Shaw and Chang, 2013)

embryonic/larval brain | third instar larval stage, with Scer\GAL4^179Y

(Pirooznia et al., 2012)

embryonic/larval mushroom body | third instar larval stage, with Scer\GAL4^ey-OK107

(Panikker et al., 2018)

embryonic/larval neuromuscular junction | third instar larval stage, with Scer\GAL4^elav.PU

(Shaw and Chang, 2013)

eye, with Scer\GAL4^GMR.PU

(Lee et al., 2016)

eye photoreceptor cell | adult stage | progressive, with Scer\GAL4^GMR.PU

(Shaw and Chang, 2013)

labellum, with Scer\GAL4^Gr33a-GAL4

(Hu et al., 2015)

larval multidendritic class IV neuron, with Scer\GAL4^ppk.PU

(Pizzano et al., 2023)

mitochondrion | third instar larval stage, with Scer\GAL4^elav.PU

(Shaw and Chang, 2013)

neuron, with Scer\GAL4^Gr33a-GAL4

(Hu et al., 2015)

NMJ bouton | increased number | third instar larval stage, with Scer\GAL4^elav.PU

(Shaw and Chang, 2013)

prothoracic tarsal segment 4, with Scer\GAL4^Gr33a-GAL4

(Hu et al., 2015)

prothoracic tarsal segment 5, with Scer\GAL4^Gr33a-GAL4

(Hu et al., 2015)

retina | adult stage | progressive, with Scer\GAL4^GMR.PU

(Shaw and Chang, 2013)

synaptic vesicle | third instar larval stage, with Scer\GAL4^elav.PU

(Shaw and Chang, 2013)

Detailed Description

Statement

Reference

The expression of Hsap\APP^{695.UAS.Tag:MYC} under the control of Scer\GAL4^elav-C155 leads to third instar larvae presenting severely decreased olfactory learning and memory, despite of apparently normal naive olfactory and gustatory responses, as compared to controls. Expression under the control of Scer\GAL4^ey-OK107 leads to a significant decrease in the size of the larval mushroom body size, but not of the (7 days old) adult mushroom body or of the overall larval or (7 days old) adult brain, as compared to controls.

(Panikker et al., 2018)

Expression of Hsap\APP^{695.Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^GMR.PU results in a mild rough-eye phenotype.

Expression of Hsap\APP^{695.Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^elav.PU results in significantly decreased survival rate during development but adult survival rate under oxidative stress conditions is not affected compared to controls.

(Lee et al., 2016)

Flies expressing Hsap\APP^{695.Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^ey-OK107 exhibit abnormal morphology of the mushroom body, with both the alpha- and beta-lobes being significantly thinner and shorter as compared to controls. These flies also show a loss of mushroom body neuroadaptive response to environmental enrichment in the first 5 days of adulthood, in contrast to wild type flies, which exhibit an increase in mushroom body size when housed with other flies during the first 5 days of adulthood, versus those kept in isolation.

(Xu et al., 2016)

Males expressing Hsap\APP^{695.Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^Gr33a-GAL4 show no difference in courtship intensity as compared with controls, but lose preference for younger females, instead showing no significant preference for either younger or older females; and also display loss of neurons in labella and foreleg tarsi.

(Hu et al., 2015)

Expression of Hsap\APP^{695.Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^c739 results in severe short term memory defects in flies throughout adulthood in a pavlovian olfactory conditioning context. These flies also show memory acquisition and retention defects in a 10-trial spaced training protocol, with severely reduced performance both immediately and 24 h after training. Aged (30-33 day old and 45 day old) flies fed the calcineurin inhibitors cyclosporin A or FK506 exhibited significant rescue of short term memory defects as compared to age-matched controls.

Expression of Hsap\APP^{695.Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^c739 does not affect gross morphology of the mushroom body.

Expression of Hsap\APP^{695.Scer\UAS.T:Hsap\MYC} under the control of transient activation of Scer\GAL4^{Mef2.247.Switch} results in short term memory defects in both young (2-4 day old) and old (30-33 day old) adult flies in a pavlovian olfactory conditioning context.

(Shaw et al., 2015)

No body wall contraction defects are seen in third instar larvae expressing Hsap\APP^{695.Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^elav-C155. Crawling distance and rate are similar to controls.

(Mhatre et al., 2014)

Scer\GAL4^ey-OK107>Hsap\APP^{695.Scer\UAS.T:Hsap\MYC} male flies do not show normal learning in a conditioned courtship suppression assay (placed with a mated female for 60 minutes), with no significant drop (unlike wild type) in courtship index when comparing the first 10 minutes to the final 10 minutes. Two minutes after training, flies are placed with a virgin female and courtship is assessed for 10 minutes: trained controls show significantly reduced courtship index when compared to sham (untrained males), whereas Scer\GAL4^ey-OK107>Hsap\APP^{695.Scer\UAS.T:Hsap\MYC} males show no significant drop (immediate-recall memory defect).

(Xu et al., 2014)

Third instar larvae expressing Hsap\APP^{695.Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^elav-C155 are significantly less mobile and perform significantly fewer full body peristaltic contractions in a locomotor assay compared to controls. The time taken for the larvae to right themselves after being turned ventral side up is also significantly longer than normal.

Larvae expressing Hsap\APP^{695.Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^elav-C155 show abnormal aggregations of vesicles on motor neurons extending from the central nervous system.

(Johnson et al., 2013)

Expression of Hsap\APP^{695.Scer\UAS.T:Hsap\MYC} driven by Scer\GAL4^GMR.PU causes age-dependent degeneration (similar to controls at day 1, with a significant percentage of the area of the retina lost and appearance of vacuoles at day 15 and even more so at day 45) of photoreceptor neurons; mutant flies also show progressive loss of phototaxis (significant decrease in the percentage of phototactic flies at day 15 and 30 but not day 1 compared to controls).

Expression of Hsap\APP^{695.Scer\UAS.T:Hsap\MYC} driven by Scer\GAL4^GMR.PU causes the formation of Hsap\APP aggregates in photoreceptor axons (axonal transport abnormalities, detected at day 45 in adults).

Expression of Hsap\APP^{695.Scer\UAS.T:Hsap\MYC} driven by Scer\GAL4^elav.PU causes accumulation of synaptic vesicles in axons (significant increase in co-localized Syt1 Hsap\APP aggregates, and the percentage and speed of Syt1 vesicles or mitochondria moving in retrograde or anterograde directions is significantly reduced) at the neuromuscular junction of third instar larvae; larvae also show significant locomotor impairments. Expression of Hsap\APP^{695.Scer\UAS.T:Hsap\MYC} driven by Scer\GAL4^elav.PU does not affect distribution or density of mitochondria, or microtubule integrity, at the third instar larval NMJ.

Scer\GAL4^elav.PU>Hsap\APP^{695.Scer\UAS.T:Hsap\MYC} larvae show significant increases in bouton number (synapse proliferation) and satellite boutons at the NMJ.

(Shaw and Chang, 2013)

Expression of Hsap\APP^{695.Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^P2.4.Pdf has no significant effect on the axonal pattern of the s-LNv neurons either in third instar larval or in adult brains but it leads to disrupted sleep pattern in adult flies compared to controls: nighttime sleep is significantly reduced with concomitant increase in daytime sleep.

(Pirooznia et al., 2012)

Ubiquitous induction of Hsap\APP^{695.Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^337Y results in 60% lethality that occurs in the pupal stage, with the remaining 40% of progeny surviving only 2-5 days after eclosion.

Moderate levels of apoptotic cell death are observed in third instar larval brains expressing Scer\GAL4^179Y>Hsap\APP^{695.Scer\UAS.T:Hsap\MYC}.

(Pirooznia et al., 2012)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Hsap\APP^{695.UAS.Tag:MYC}, Scer\GAL4^ey-OK107 has abnormal neuroanatomy | adult stage phenotype, enhanceable by Tip60^E431Q.UAS, Scer\GAL4^ey-OK107

(Xu et al., 2016)

Hsap\APP^{695.UAS.Tag:MYC}, Scer\GAL4^elav-C155 has visible phenotype, enhanceable by Dmtn^GD2834, Scer\GAL4^elav-C155

(Hopkins, 2013)

Hsap\APP^{695.UAS.Tag:MYC}, Scer\GAL4^elav-C155 has abnormal locomotor behavior | third instar larval stage phenotype, enhanceable by Tip60^E431Q.UAS, Scer\GAL4^elav-C155

(Johnson et al., 2013)

Hsap\APP^{695.UAS.Tag:MYC}, Scer\GAL4^GMR.PU has abnormal neuroanatomy | adult stage | progressive phenotype, enhanceable by sra¹

(Shaw and Chang, 2013)

Hsap\APP^{695.UAS.Tag:MYC}, Scer\GAL4^GMR.PU has abnormal phototaxis | adult stage | progressive phenotype, enhanceable by sra¹

(Shaw and Chang, 2013)

Hsap\APP^{695.UAS.Tag:MYC}, Scer\GAL4^elav.PU has abnormal locomotor behavior | third instar larval stage phenotype, enhanceable by Pp2B-14D^Act.Δ.UAS, Scer\GAL4^elav.PU

(Shaw and Chang, 2013)

NOT Enhanced by

Statement

Reference

Hsap\APP^{695.UAS.Tag:MYC}, Scer\GAL4^c739 has abnormal memory | adult stage phenotype, non-enhanceable by sra^JF02557, Scer\GAL4^c739

(Shaw et al., 2015)

Hsap\APP^{695.UAS.Tag:MYC}, Scer\GAL4^elav.PU has abnormal locomotor behavior | third instar larval stage phenotype, non-enhanceable by sra^JF02557, Scer\GAL4^elav.PU

(Shaw and Chang, 2013)

Hsap\APP^{695.UAS.Tag:MYC}, Scer\GAL4^elav.PU has abnormal neuroanatomy | third instar larval stage phenotype, non-enhanceable by sra^JF02557, Scer\GAL4^elav.PU

(Shaw and Chang, 2013)

Hsap\APP^{695.UAS.Tag:MYC}, Scer\GAL4^elav.PU has abnormal locomotor behavior | third instar larval stage phenotype, non-enhanceable by sra¹

(Shaw and Chang, 2013)

Hsap\APP^{695.UAS.Tag:MYC}, Scer\GAL4^elav.PU has abnormal neuroanatomy | third instar larval stage phenotype, non-enhanceable by sra¹

(Shaw and Chang, 2013)

Suppressed by

Statement

Reference

Hsap\APP^{695.UAS.Tag:MYC}, Scer\GAL4^elav-C155 has abnormal memory | third instar larval stage phenotype, suppressible by Tip60^UAS.cPa, Scer\GAL4^elav-C155

(Panikker et al., 2018)

Hsap\APP^{695.UAS.Tag:MYC}, Scer\GAL4^elav-C155 has abnormal learning | third instar larval stage phenotype, suppressible by Tip60^UAS.cPa, Scer\GAL4^elav-C155

(Panikker et al., 2018)

Hsap\APP^{695.UAS.Tag:MYC}, Scer\GAL4^ey-OK107 has abnormal size | third instar larval stage phenotype, suppressible by Tip60^UAS.cPa, Scer\GAL4^ey-OK107

(Panikker et al., 2018)

Hsap\APP^{695.UAS.Tag:MYC}, Scer\GAL4^elav.PU has partially lethal - majority die phenotype, suppressible | partially by sra^EY07182, Scer\GAL4^elav.PU

(Lee et al., 2016)

Hsap\APP^{695.UAS.Tag:MYC}, Scer\GAL4^ey-OK107 has abnormal neuroanatomy | adult stage phenotype, suppressible by Tip60^UAS.cLa, Scer\GAL4^ey-OK107

(Xu et al., 2016)

Hsap\APP^{695.UAS.Tag:MYC}, Scer\GAL4^Gr33a-GAL4 has abnormal courtship behavior phenotype, suppressible by foxo[+]/foxo^Δ94

(Hu et al., 2015)

Hsap\APP^{695.UAS.Tag:MYC}, Scer\GAL4^c739 has abnormal memory | adult stage phenotype, suppressible | partially by CanB^JF02616, Scer\GAL4^c739

(Shaw et al., 2015)

Hsap\APP^{695.UAS.Tag:MYC}, Scer\GAL4^c739 has abnormal memory | adult stage phenotype, suppressible | partially by Pka-C1^UAS.Tag:FLAG, Scer\GAL4^c739

(Shaw et al., 2015)

Hsap\APP^{695.UAS.Tag:MYC}, Scer\GAL4^{Mef2.247.Switch} has abnormal memory | adult stage | RU486 conditional phenotype, suppressible | RU486 conditional by sra^UAS.Tag:HA, Scer\GAL4^{Mef2.247.Switch}

(Shaw et al., 2015)

Hsap\APP^{695.UAS.Tag:MYC} has abnormal memory | adult stage phenotype, suppressible by sra^UAS.Tag:HA/Scer\GAL4^c739

(Shaw et al., 2015)

Hsap\APP^{695.UAS.Tag:MYC}, Scer\GAL4^ey-OK107 has abnormal learning | adult stage phenotype, suppressible by Tip60^UAS.cLa, Scer\GAL4^ey-OK107

(Xu et al., 2014)

Hsap\APP^{695.UAS.Tag:MYC}, Scer\GAL4^ey-OK107 has abnormal memory | adult stage phenotype, suppressible by Tip60^UAS.cLa, Scer\GAL4^ey-OK107

(Xu et al., 2014)

Hsap\APP^{695.UAS.Tag:MYC}, Scer\GAL4^elav-C155 has partially lethal - majority die phenotype, suppressible by Dmtn^UAS.cHa, Scer\GAL4^elav-C155

(Hopkins, 2013)

Hsap\APP^{695.UAS.Tag:MYC}, Scer\GAL4^elav-C155 has visible phenotype, suppressible by Dmtn^UAS.cHa, Scer\GAL4^elav-C155

(Hopkins, 2013)

Hsap\APP^{695.UAS.Tag:MYC}, Scer\GAL4^elav-C155 has partially lethal - majority die phenotype, suppressible by Dmtn^GD2834, Scer\GAL4^elav-C155

(Hopkins, 2013)

Hsap\APP^{695.UAS.Tag:MYC}, Scer\GAL4^GMR.PU has abnormal neuroanatomy | adult stage | progressive phenotype, suppressible | partially by sra^UAS.Tag:HA, Scer\GAL4^GMR.PU

(Shaw and Chang, 2013)

Hsap\APP^{695.UAS.Tag:MYC}, Scer\GAL4^GMR.PU has abnormal phototaxis | adult stage | progressive phenotype, suppressible by sra^UAS.Tag:HA, Scer\GAL4^GMR.PU

(Shaw and Chang, 2013)

Hsap\APP^{695.UAS.Tag:MYC}, Scer\GAL4^elav.PU has abnormal locomotor behavior | third instar larval stage phenotype, suppressible | partially by sra^UAS.Tag:HA, Scer\GAL4^elav.PU

(Shaw and Chang, 2013)

Hsap\APP^{695.UAS.Tag:MYC}, Scer\GAL4^elav.PU has abnormal locomotor behavior | third instar larval stage phenotype, suppressible by CanB^JF02616, Scer\GAL4^elav.PU

(Shaw and Chang, 2013)

Hsap\APP^{695.UAS.Tag:MYC}, Scer\GAL4^elav.PU, sra^UAS.Tag:HA has abnormal locomotor behavior | third instar larval stage phenotype, suppressible | partially by Pp2B-14D^Act.Δ.UAS, Scer\GAL4^elav.PU

(Shaw and Chang, 2013)

Hsap\APP^{695.UAS.Tag:MYC}, Scer\GAL4^P2.4.Pdf has abnormal sleep | adult stage phenotype, suppressible by Tip60^UAS.cLa, Scer\GAL4^P2.4.Pdf

(Pirooznia et al., 2012)

Hsap\APP^{695.UAS.Tag:MYC}, Scer\GAL4^179Y has increased cell death | third instar larval stage phenotype, suppressible | partially by Tip60^UAS.cPa, Scer\GAL4^179Y

(Pirooznia et al., 2012)

Hsap\APP^{695.UAS.Tag:MYC}, Scer\GAL4^337Y has majority die during P-stage phenotype, suppressible | partially by Tip60^UAS.cPa, Scer\GAL4^337Y

(Pirooznia et al., 2012)

Hsap\APP^{695.UAS.Tag:MYC}, Scer\GAL4^179Y has majority die during P-stage phenotype, suppressible | partially by Tip60^UAS.cPa, Scer\GAL4^179Y

(Pirooznia et al., 2012)

NOT suppressed by

Statement

Reference

Hsap\APP^{695.UAS.Tag:MYC}, Scer\GAL4^c739 has abnormal memory | adult stage phenotype, non-suppressible by sra^JF02557, Scer\GAL4^c739

(Shaw et al., 2015)

Hsap\APP^{695.UAS.Tag:MYC}, Scer\GAL4^c739 has abnormal memory | adult stage phenotype, non-suppressible by sgg¹

(Shaw et al., 2015)

Hsap\APP^{695.UAS.Tag:MYC}, Scer\GAL4^ey-OK107 has abnormal learning | adult stage phenotype, non-suppressible by Tip60^E431Q.UAS, Scer\GAL4^ey-OK107

(Xu et al., 2014)

Hsap\APP^{695.UAS.Tag:MYC}, Scer\GAL4^ey-OK107 has abnormal memory | adult stage phenotype, non-suppressible by Tip60^E431Q.UAS, Scer\GAL4^ey-OK107

(Xu et al., 2014)

Enhancer of

Statement

Reference

Hsap\APP^{695.UAS.Tag:MYC}, Scer\GAL4^elav-C155 is an enhancer of abnormal locomotor behavior | third instar larval stage phenotype of Hsap\BACE1^UAS.Exel, Scer\GAL4^elav-C155

(Mhatre et al., 2014)

Hsap\APP^{695.UAS.Tag:MYC}, Scer\GAL4^elav-C155 is an enhancer of abnormal locomotor behavior | third instar larval stage phenotype of Scer\GAL4^elav-C155, Tip60^E431Q.UAS

(Johnson et al., 2013)

Hsap\APP^{695.UAS.Tag:MYC}, Scer\GAL4^P2.4.Pdf is an enhancer of abnormal neuroanatomy | adult stage phenotype of Scer\GAL4^P2.4.Pdf, Tip60^E431Q.UAS

(Pirooznia et al., 2012)

Hsap\APP^{695.UAS.Tag:MYC}, Scer\GAL4^179Y is an enhancer of increased mortality during development phenotype of Scer\GAL4^179Y, Tip60^E431Q.UAS

(Pirooznia et al., 2012)

Hsap\APP^{695.UAS.Tag:MYC}, Scer\GAL4^179Y is an enhancer of increased cell death | third instar larval stage phenotype of Scer\GAL4^179Y, Tip60^E431Q.UAS

(Pirooznia et al., 2012)

Hsap\APP^{695.UAS.Tag:MYC}, Scer\GAL4^337Y is an enhancer of increased mortality during development phenotype of Scer\GAL4^337Y, Tip60^E431Q.UAS

(Pirooznia et al., 2012)

NOT Enhancer of

Statement

Reference

Hsap\APP^{695.UAS.Tag:MYC}, Scer\GAL4^c739 is a non-enhancer of abnormal memory | adult stage phenotype of Scer\GAL4^c739, sra^JF02557

(Shaw et al., 2015)

Suppressor of

Statement

Reference

Hsap\APP^{695.UAS.Tag:MYC}, Scer\GAL4^elav.PU is a suppressor of abnormal oxidative stress response | adult stage phenotype of Scer\GAL4^elav.PU, sra^EY07182

(Lee et al., 2016)

Hsap\APP^{695.UAS.Tag:MYC}, Scer\GAL4^c739 is a suppressor | partially of abnormal memory | adult stage phenotype of Scer\GAL4^c739, sra^UAS.Tag:HA

(Shaw et al., 2015)

Hsap\APP^{695.UAS.Tag:MYC}, Scer\GAL4^{Mef2.247.Switch} is a suppressor | RU486 conditional of abnormal memory | adult stage | RU486 conditional phenotype of Scer\GAL4^{Mef2.247.Switch}, sra^UAS.Tag:HA

(Shaw et al., 2015)

Hsap\APP^{695.UAS.Tag:MYC}, Scer\GAL4^P2.4.Pdf is a suppressor of abnormal sleep | adult stage phenotype of Scer\GAL4^P2.4.Pdf, Tip60^UAS.cLa

(Pirooznia et al., 2012)

NOT Suppressor of

Statement

Reference

Hsap\APP^{695.UAS.Tag:MYC}, Scer\GAL4^c739 is a non-suppressor of abnormal memory | adult stage phenotype of Scer\GAL4^c739, sra^JF02557

(Shaw et al., 2015)

Hsap\APP^{695.UAS.Tag:MYC}, Scer\GAL4^337Y is a non-suppressor of partially lethal - majority die phenotype of Scer\GAL4^337Y, Tip60^UAS.cPa

(Pirooznia et al., 2012)

Hsap\APP^{695.UAS.Tag:MYC}, Scer\GAL4^179Y is a non-suppressor of partially lethal - majority die phenotype of Scer\GAL4^179Y, Tip60^UAS.cPa

(Pirooznia et al., 2012)

Other

Statement

Reference

Phenotype Manifest In

Enhanced by

Statement

Reference

Hsap\APP^{695.UAS.Tag:MYC}, Scer\GAL4^ey-OK107 has adult mushroom body phenotype, enhanceable by Tip60^E431Q.UAS, Scer\GAL4^ey-OK107

(Xu et al., 2016)

Hsap\APP^{695.UAS.Tag:MYC}, Scer\GAL4^elav-C155 has wing phenotype, enhanceable by Dmtn^GD2834, Scer\GAL4^elav-C155

(Hopkins, 2013)

Hsap\APP^{695.UAS.Tag:MYC}, Scer\GAL4^GMR.PU has retina | adult stage | progressive phenotype, enhanceable by sra¹

(Shaw and Chang, 2013)

Hsap\APP^{695.UAS.Tag:MYC}, Scer\GAL4^elav.PU has synaptic vesicle | third instar larval stage phenotype, enhanceable by sra^JF02557, Scer\GAL4^elav.PU

(Shaw and Chang, 2013)

Hsap\APP^{695.UAS.Tag:MYC}, Scer\GAL4^elav.PU has embryonic/larval neuromuscular junction | third instar larval stage phenotype, enhanceable by sra^JF02557, Scer\GAL4^elav.PU

(Shaw and Chang, 2013)

Hsap\APP^{695.UAS.Tag:MYC}, Scer\GAL4^elav.PU has axon | third instar larval stage phenotype, enhanceable by sra^JF02557, Scer\GAL4^elav.PU

(Shaw and Chang, 2013)

Hsap\APP^{695.UAS.Tag:MYC}, Scer\GAL4^elav.PU has axon | third instar larval stage phenotype, enhanceable by Pp2B-14D^Act.Δ.UAS, Scer\GAL4^elav.PU

(Shaw and Chang, 2013)

Hsap\APP^{695.UAS.Tag:MYC}, Scer\GAL4^elav.PU has synaptic vesicle | third instar larval stage phenotype, enhanceable by Pp2B-14D^Act.Δ.UAS, Scer\GAL4^elav.PU

(Shaw and Chang, 2013)

NOT Enhanced by

Statement

Reference

Hsap\APP^{695.UAS.Tag:MYC}, Scer\GAL4^elav.PU has NMJ bouton | third instar larval stage | increased number phenotype, non-enhanceable by sra^JF02557, Scer\GAL4^elav.PU

(Shaw and Chang, 2013)

Hsap\APP^{695.UAS.Tag:MYC}, Scer\GAL4^elav.PU has NMJ bouton | third instar larval stage | increased number phenotype, non-enhanceable by sra¹

(Shaw and Chang, 2013)

Hsap\APP^{695.UAS.Tag:MYC}, Scer\GAL4^elav.PU has axon | third instar larval stage phenotype, non-enhanceable by sgg^S9A.UAS, Scer\GAL4^elav.PU

(Shaw and Chang, 2013)

Hsap\APP^{695.UAS.Tag:MYC}, Scer\GAL4^elav.PU has synaptic vesicle | third instar larval stage phenotype, non-enhanceable by sgg^S9A.UAS, Scer\GAL4^elav.PU

(Shaw and Chang, 2013)

Suppressed by

Statement

Reference

Hsap\APP^{695.UAS.Tag:MYC}, Scer\GAL4^ey-OK107 has embryonic/larval mushroom body | third instar larval stage phenotype, suppressible by Tip60^UAS.cPa, Scer\GAL4^ey-OK107

(Panikker et al., 2018)

Hsap\APP^{695.UAS.Tag:MYC}, Scer\GAL4^ey-OK107 has adult mushroom body phenotype, suppressible by Tip60^UAS.cLa, Scer\GAL4^ey-OK107

(Xu et al., 2016)

Hsap\APP^{695.UAS.Tag:MYC}, Scer\GAL4^elav-C155 has wing phenotype, suppressible by Dmtn^UAS.cHa, Scer\GAL4^elav-C155

(Hopkins, 2013)

Hsap\APP^{695.UAS.Tag:MYC}, Scer\GAL4^GMR.PU has eye photoreceptor cell | adult stage | progressive phenotype, suppressible by sra^UAS.Tag:HA, Scer\GAL4^GMR.PU

(Shaw and Chang, 2013)

Hsap\APP^{695.UAS.Tag:MYC}, Scer\GAL4^GMR.PU has axon | adult stage | progressive phenotype, suppressible by sra^UAS.Tag:HA, Scer\GAL4^GMR.PU

(Shaw and Chang, 2013)

Hsap\APP^{695.UAS.Tag:MYC}, Scer\GAL4^GMR.PU has retina | adult stage | progressive phenotype, suppressible | partially by sra^UAS.Tag:HA, Scer\GAL4^GMR.PU

(Shaw and Chang, 2013)

Hsap\APP^{695.UAS.Tag:MYC}, Scer\GAL4^elav.PU has synaptic vesicle | third instar larval stage phenotype, suppressible | partially by sra^UAS.Tag:HA, Scer\GAL4^elav.PU

(Shaw and Chang, 2013)

Hsap\APP^{695.UAS.Tag:MYC}, Scer\GAL4^elav.PU has embryonic/larval neuromuscular junction | third instar larval stage phenotype, suppressible | partially by sra^UAS.Tag:HA, Scer\GAL4^elav.PU

(Shaw and Chang, 2013)

Hsap\APP^{695.UAS.Tag:MYC}, Scer\GAL4^elav.PU has axon | third instar larval stage phenotype, suppressible | partially by sra^UAS.Tag:HA, Scer\GAL4^elav.PU

(Shaw and Chang, 2013)

Hsap\APP^{695.UAS.Tag:MYC}, Scer\GAL4^elav.PU has mitochondrion | third instar larval stage phenotype, suppressible | partially by sra^UAS.Tag:HA, Scer\GAL4^elav.PU

(Shaw and Chang, 2013)

Hsap\APP^{695.UAS.Tag:MYC}, Scer\GAL4^elav.PU has axon | third instar larval stage phenotype, suppressible by CanB^JF02616, Scer\GAL4^elav.PU

(Shaw and Chang, 2013)

Hsap\APP^{695.UAS.Tag:MYC}, Scer\GAL4^elav.PU has synaptic vesicle | third instar larval stage phenotype, suppressible by CanB^JF02616, Scer\GAL4^elav.PU

(Shaw and Chang, 2013)

Hsap\APP^{695.UAS.Tag:MYC}, Scer\GAL4^elav.PU, sra^UAS.Tag:HA has axon | third instar larval stage phenotype, suppressible | partially by Pp2B-14D^Act.Δ.UAS, Scer\GAL4^elav.PU

(Shaw and Chang, 2013)

Hsap\APP^{695.UAS.Tag:MYC}, Scer\GAL4^elav.PU, sra^UAS.Tag:HA has synaptic vesicle | third instar larval stage phenotype, suppressible | partially by Pp2B-14D^Act.Δ.UAS, Scer\GAL4^elav.PU

(Shaw and Chang, 2013)

Hsap\APP^{695.UAS.Tag:MYC}, Scer\GAL4^elav.PU has axon | third instar larval stage phenotype, suppressible by sgg¹

(Shaw and Chang, 2013)

Hsap\APP^{695.UAS.Tag:MYC}, Scer\GAL4^elav.PU has synaptic vesicle | third instar larval stage phenotype, suppressible by sgg¹

(Shaw and Chang, 2013)

Hsap\APP^{695.UAS.Tag:MYC}, Pp2B-14D^Act.Δ.UAS, Scer\GAL4^elav.PU has axon | third instar larval stage phenotype, suppressible by sgg¹

(Shaw and Chang, 2013)

Hsap\APP^{695.UAS.Tag:MYC}, Pp2B-14D^Act.Δ.UAS, Scer\GAL4^elav.PU has synaptic vesicle | third instar larval stage phenotype, suppressible by sgg¹

(Shaw and Chang, 2013)

Hsap\APP^{695.UAS.Tag:MYC}, Scer\GAL4^179Y has embryonic/larval brain | third instar larval stage phenotype, suppressible | partially by Tip60^UAS.cPa, Scer\GAL4^179Y

(Pirooznia et al., 2012)

Enhancer of

Statement

Reference

Hsap\APP^{695.UAS.Tag:MYC}, Scer\GAL4^P2.4.Pdf is an enhancer of s-LNv Pdf neuron | adult stage phenotype of Scer\GAL4^P2.4.Pdf, Tip60^E431Q.UAS

(Pirooznia et al., 2012)

Hsap\APP^{695.UAS.Tag:MYC}, Scer\GAL4^P2.4.Pdf is an enhancer of axon | adult stage phenotype of Scer\GAL4^P2.4.Pdf, Tip60^E431Q.UAS

(Pirooznia et al., 2012)

Hsap\APP^{695.UAS.Tag:MYC}, Scer\GAL4^179Y is an enhancer of embryonic/larval brain | third instar larval stage phenotype of Scer\GAL4^179Y, Tip60^E431Q.UAS

(Pirooznia et al., 2012)

Other

Statement

Reference

Hsap\APP^{695.UAS.Tag:MYC}, Hsap\BACE1^UAS.Exel, Scer\GAL4^elav-C155 has type I bouton phenotype

(Mhatre et al., 2014)

Hsap\APP^{695.UAS.Tag:MYC}, Scer\GAL4^elav.PU, sra^UAS.Tag:HA has NMJ bouton | third instar larval stage phenotype

(Shaw and Chang, 2013)

Hsap\APP^{695.UAS.Tag:MYC}, Scer\GAL4^P2.4.Pdf, Tip60^UAS.cLa has axon | adult stage phenotype

(Pirooznia et al., 2012)

Hsap\APP^{695.UAS.Tag:MYC}, Scer\GAL4^P2.4.Pdf, Tip60^UAS.cLa has s-LNv Pdf neuron | adult stage phenotype

(Pirooznia et al., 2012)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

The decreased olfactory learning and memory exhibited by third instar larvae upon the expression of Hsap\APP^{695.UAS.Tag:MYC} under the control of Scer\GAL4^elav-C155 is partially suppressed by the co-expression of Tip60^UAS.cPa. The smaller larval mushroom body induced by the expression of Hsap\APP^{695.UAS.Tag:MYC} under the control of Scer\GAL4^ey-OK107 is also partially suppressed by the co-expression of Tip60^UAS.cPa.

(Panikker et al., 2018)

The increased mortality during development characteristic for adult flies expressing Hsap\APP^{695.Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^elav.PU is improved by co-expression of sra^EY07182.

(Lee et al., 2016)

Co-expression of Tip60^Scer\UAS.cLa suppresses the mushroom body morphological defects, observed in flies expressing Hsap\APP^{695.Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^ey-OK107. The mushroom body neuroadaptive response to environmental enrichment is also restored in these flies.

Co-expression of Tip60^{E431Q.Scer\UAS} exacerbates axonal defects in all three mushroom body lobes, resulting in a lack of structural consistency of the mushroom body in flies expressing Hsap\APP^{695.Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^ey-OK107.

(Xu et al., 2016)

The younger female preference defect of males expressing Hsap\APP^{695.Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^Gr33a-GAL4 is significantly rescued in the presence of the foxo^Δ94/+ mutation.

(Hu et al., 2015)

Co-expression of CanB^JF02616 significantly rescues the short term memory defects of flies expressing Hsap\APP^{695.Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^c739.

Introducing the sgg¹ mutation to flies expressing Hsap\APP^{695.Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^c739 does not result in any rescue of short term memory defects.

Co-expression of Pka-C1^{Scer\UAS.T:Zzzz\FLAG} significantly rescues the short term memory defects of flies expressing Hsap\APP^{695.Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^c739.

Co-expression of sra^Scer\UAS.cCa fully rescues the short term memory defects of flies expressing Hsap\APP^{695.Scer\UAS.T:Hsap\MYC} under the control of transient activation of Scer\GAL4^{Mef2.247.Switch}.

Co-expression of sra^Scer\UAS.cCa and Hsap\APP^{695.Scer\UAS.T:Hsap\MYC} (under the control of Scer\GAL4^c739) fully rescues the short term memory defect in young (< 10 day old) adult flies, but not in older (> 30 day old) flies, as compared to flies with either construct alone, and does not affect gross morphology of the mushroom body.

Co-expression of sra^Scer\UAS.cCa partially rescues performance after 10-trial spaced training in flies with Hsap\APP^{695.Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^c739.

Co-expression of Hsap\APP^{695.Scer\UAS.T:Hsap\MYC} and sra^JF02557 (under the control of Scer\GAL4^c739) results in a similar performance index in short term memory assays as expression of either construct alone.

(Shaw et al., 2015)

Larvae co-expressing Hsap\APP^{695.Scer\UAS.T:Hsap\MYC} and Hsap\BACE1^{Scer\UAS.Exel} under the control of Scer\GAL4^elav-C155 show a significant decrease in third instar larval body wall contractions.

The crawling defects seen when Hsap\BACE1^{Scer\UAS.Exel} is expressed under the control of Scer\GAL4^elav-C155 are enhanced upon co-expression of Hsap\APP^{695.Scer\UAS.T:Hsap\MYC}.

The body wall contraction and crawling phenotypes seen in flies co-expressing Hsap\APP^{695.Scer\UAS.T:Hsap\MYC} and Hsap\BACE1^{Scer\UAS.Exel} under the control of Scer\GAL4^elav-C155 on standard food are partially rescued when the flies are raised on food containing the γ-secretase inhibitor L-685,458.

Co-expression of Hsap\APP^{695.Scer\UAS.T:Hsap\MYC} and Hsap\BACE1^{Scer\UAS.Exel} under the control of Scer\GAL4^elav-C155 results in structural changes in the synapse of the muscle 6 and 7 NMJ. These structural changes are rescued when the flies are raised on the γ-secretase inhibitor L-685,458. The overall number of boutons is reduced compared to controls, with reductions in the numbers of both 1b and 1s boutons. The 1s bouton phenotype is significantly rescued by L-685,458, and the 1b phenotype is partially rescued. The total area of motor neuron innervation in flies expressing Hsap\APP^{695.Scer\UAS.T:Hsap\MYC} and Hsap\BACE1^{Scer\UAS.Exel} is similar to controls, but a significant reduction is seen in the amount of motor neuron branching, as well as the size of muscles 6 and 7 at the NMJ. Feeding the larvae L-685,458 suppresses the branching defect, but has no effect on the reduction in muscle size. The density of presynaptic release sites (brp-positive active zones) is normal, both on standard food or L-685,458. Mitochondrial localisation defects are seen in motor neurons and this is also rescued by L-685,458.

(Mhatre et al., 2014)

Co-expression of Tip60^Scer\UAS.cLa (but not Tip60^{E431Q.Scer\UAS}) suppresses learning and memory defects in Scer\GAL4^ey-OK107>Hsap\APP^{695.Scer\UAS.T:Hsap\MYC} males.

(Xu et al., 2014)

Expression of Dmtn^Scer\UAS.cHa suppresses the predominantly male semi-lethality and wing expansion defects seen when Hsap\APP^{695.Scer\UAS.T:Hsap\MYC} is expressed in neurons under the control of Scer\GAL4^elav-C155.

Expression of Dmtn^GD2834 partially suppresses the predominantly male semi-lethality seen when Hsap\APP^{695.Scer\UAS.T:Hsap\MYC} is expressed in neurons under the control of Scer\GAL4^elav-C155 (co-expressed with Dcr-2^Scer\UAS.cDa). The wing expansion defects are enhanced compared to expression of Dmtn^GD2834 alone.

(Hopkins, 2013)

Larvae co-expressing both Tip60^{E431Q.Scer\UAS} and Hsap\APP^{695.Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^elav-C155 show a synergistic enhancement of the locomotor defects that are seen in larvae expressing either Tip60^{E431Q.Scer\UAS} or Hsap\APP^{695.Scer\UAS.T:Hsap\MYC} alone. In addition, the formation of axonal vesicle accumulations is exacerbated in the double mutants.

Co-expression of Tip60^Scer\UAS.cLa partially suppresses the locomotor defects and vesicle accumulation on motor axons that is seen in third instar larvae expressing Hsap\APP^{695.Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^elav-C155.

(Johnson et al., 2013)

Co-expression of sra^{Scer\UAS.T:Ivir\HA1} significantly delays the onset of age-dependent retinal degeneration and suppresses age-dependent phototaxis defects in flies with expression of Hsap\APP^{695.Scer\UAS.T:Hsap\MYC} driven by Scer\GAL4^GMR.PU. sra¹ significantly enhances age-dependent retinal degeneration and phototaxis defects in flies with expression of Hsap\APP^{695.Scer\UAS.T:Hsap\MYC} driven by Scer\GAL4^GMR.PU. Co-expression of sra^{Scer\UAS.T:Ivir\HA1} suppresses formation of Hsap\APP aggregates in photoreceptor axons in flies with Hsap\APP^{695.Scer\UAS.T:Hsap\MYC} driven by Scer\GAL4^GMR.PU, likely alleviating blocked transport to synaptic terminals in the medulla.

sra¹ slightly (but not significantly) increases synaptic vesicle accumulation (aggregates) seen in axons at the neuromuscular junction (and does not enhance locomotor defects) in third instar larvae with expression of Hsap\APP^{695.Scer\UAS.T:Hsap\MYC} driven by Scer\GAL4^elav.PU. Co-expression of sra^JF02557 significantly enhances aggregate formation in axons at the NMJ (but does not enhance locomotor defects) of Scer\GAL4^elav.PU>Hsap\APP^{695.Scer\UAS.T:Hsap\MYC} larvae; Syt1 anterograde and retrograde transport and speed is also reduced in larval motor axons. Co-expression of sra^{Scer\UAS.T:Ivir\HA1} significantly partially suppresses aggregate formation in axons and locomotion defects at the Scer\GAL4^elav.PU>Hsap\APP^{695.Scer\UAS.T:Hsap\MYC} third instar larval NMJ; Syt1 vesicle or mitochondrial anterograde and retrograde transport is also facilitated in larval motor axons.

Co-expression of sra^{Scer\UAS.T:Ivir\HA1} significantly suppresses the increases in bouton number (synapse proliferation), but not satellite bouton number at the NMJ in Scer\GAL4^elav.PU>Hsap\APP^{695.Scer\UAS.T:Hsap\MYC} larvae. Co-expression of sra^JF02557 or presence of sra¹ does not enhance bouton number (synapse proliferation) or satellite bouton number at the NMJ in Scer\GAL4^elav.PU>Hsap\APP^{695.Scer\UAS.T:Hsap\MYC} larvae.

Co-expression of CanB^JF02616 reduces Syt1 aggregate accumulations at NMJ axons and restores locomotor behavior in Scer\GAL4^elav.PU>Hsap\APP^{695.Scer\UAS.T:Hsap\MYC} larvae; co-expression of Pp2B-14D^{Act.Δ.Scer\UAS} exacerbates these phenotypes. Co-expression of Pp2B-14D^{Act.Δ.Scer\UAS} suppresses the ability of sra^{Scer\UAS.T:Ivir\HA1} to protect against Scer\GAL4^elav.PU>Hsap\APP^{695.Scer\UAS.T:Hsap\MYC} induced larval axonal transport and locomotion defects.

sgg¹ suppresses Syt1 aggregation in motor axons in the NMJ of Scer\GAL4^elav.PU>Hsap\APP^{695.Scer\UAS.T:Hsap\MYC} third instar larvae. sgg¹ suppresses the effect of Pp2B-14D^{Act.Δ.Scer\UAS} exacerbating the aggregation in motor axons in the NMJ of Scer\GAL4^elav.PU>Hsap\APP^{695.Scer\UAS.T:Hsap\MYC} third instar larvae. Co-expression of sgg^S9A.Scer\UAS does not affect Syt1 aggregation in motor axons in the NMJ of Scer\GAL4^elav.PU>Hsap\APP^{695.Scer\UAS.T:Hsap\MYC} third instar larvae.

(Shaw and Chang, 2013)

The defects in the axonal pattern of s-LNv neurons in brains characteristic for adult flies expressing Tip60^{E431Q.Scer\UAS} under the control of Scer\GAL4^P2.4.Pdf are exacerbated further by co-expression of Hsap\APP^{695.Scer\UAS.T:Hsap\MYC} and flies co-expressing Tip60^{E431Q.Scer\UAS} with Hsap\APP^{695.Scer\UAS.T:Hsap\MYC} show reduction in nighttime sleep with concomitant increase in daytime sleep (also seen in flies expressing either Tip60^{E431Q.Scer\UAS} or Hsap\APP^{695.Scer\UAS.T:Hsap\MYC} alone).

Simultaneous expression of Tip60^Scer\UAS.cLa with Hsap\APP^{695.Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^P2.4.Pdf has no effect on the axonal pattern of the s-LNv neurons in third instar larval brain, while it leads to a large increase in the area of the s-LNv neuron axonal arbor in the adult brain and it also rescues the nighttime sleep defects observed in adult flies expressing either of the transgenes alone.

(Pirooznia et al., 2012)

Co-expression of Tip60^{E431Q.Scer\UAS} and Hsap\APP^{695.Scer\UAS.T:Hsap\MYC} both under the control of Scer\GAL4^337Y results in 0% viability, with lethality occurring during the early second instar larval stage. Additionally, hatching of 100% of these larvae is delayed by 24-48 hours.

(Pirooznia et al., 2012)

Complementation and Rescue Data

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Mutant

Wild-type

Stocks (3)

Bloomington

6700

w^*; P{UAS-APP695-N-myc}TW6

33798

w¹¹¹⁸; P{UAS-APP695-N-myc}TW6, P{UAS-BACE1.Exel}1b