From kcook@XXXX Mon Oct 07  18:04:07  2002
To: Rachel Drysdale (Genetics) <rd120@XXXX>
Subject: APP constructs and insertions
The following information was provided by Gunter Merdes, University of
Heidelberg (9/02).
P{UAS-Hsap\APP.695SPA4CT. T:Myc }XX and P{UAS-Hsap\APP.695DeltaCT. T:Myc }61
are homozygous viable and fertile second chromosome insertions. Their
construction and transformation were described in Fossgreen et al. (PNAS
95: 13703-13708, 1998; FBrf0105227).
After Fossgreen et al. was published, constructs similar to
P{UAS-Hsap\APP.695} and P{UAS-Hsap\APP.695-Swedish} were made with
N-terminal Myc tags. The constructs are P{UAS-APP695-N-myc} and
P{UAS-APP695-Swedish-N-myc}.
P{UAS-APP695-N-myc}TW6 is a homozygous viable and fertile second
chromosome insertion.
P{UAS-APP695-Swedish-N-myc}TS23 is a homozygous viable and fertile
third chromosome insertion.
__________________________________________________________
Kevin Cook, Ph.D. Bloomington Drosophila Stock Center
Department of Biology http://flystocks.bio.indiana.edu
Jordan Hall 142
Indiana University 812-855-5782
1001 E. Third St. 812-855-2577 (fax)
Bloomington, IN 47405-3700 kcook@XXXX