FlyBase Allele Report: Dmel\Notum[3]

General Information

Symbol

Dmel\Notum³

Species

D. melanogaster

Name

FlyBase ID

FBal0138446

Feature type

allele

Associated gene

Dmel\Notum

Associated Insertion(s)

P{EPg}Notum^EPg31259

Carried in Construct

Also Known As

EP-Notum

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

Nature of the Allele

Allele class

Mutagen

ethyl methanesulfonate

(Giraldez et al., 2002)

P-element activity

(Giraldez et al., 2002)

Progenitor genotype

Notum^EPg31259

(Giraldez et al., 2002)

Associated Insertion(s)

P{EPg}Notum^EPg31259

Cytology

Description

The P{EPg}Notum^EPg31259 insertion is also still present on the chromosome.

(Giraldez et al., 2002)

Amino acid replacement: Q485term.

(Giraldez et al., 2002)

Allele components

Component

Use(s)

Inserted element

P{EPg}

misexpression element

Regulatory region(s)

UASp

binary expression system - regulatory region

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

3L:16,030,778..16,030,778 [+]

Nucleotide change:

C16030778T

Amino acid change:

Q485term | Notum-PA

Reported amino acid change:

Q485term

Comment:

The Notum³ mutation was induced on the Notum^EPg31259 chromosome which carries a P{EPg} insertion approximately 1kb upstream of Notum. Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

(Giraldez et al., 2002)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

increased cell death | somatic clone

(Lim and Tomlinson, 2006)

visible | recessive | somatic clone

(Giraldez et al., 2002)

Phenotype Manifest In

Detailed Description

Statement

Reference

Clones of Notum³ in pupal eyes show an increase in the number of peripheral ommatidia that undergo apoptosis and a corresponding expansion of the pigment rim.

(Lim and Tomlinson, 2006)

Homozygous embryos derived from females containing homozygous germline clones have a range of severe defects. Most of these embryos have abnormal morphology; 43% have a posterior end that is not completely retracted, 12% are U-shaped and 34% are shaped like a ball. Of those embryos where the ventral cuticle can be reliably analysed, nearly all have thinner denticle belts in the abdomen than normal, and many have either patches of naked cuticle within the denticle belts, or missing denticle belts. Paternally rescued embryos derived from females containing homozygous germline clones hatch and do not show any morphological defects.

(Desbordes et al., 2005)

Notum³/Notum^I6 larval wing discs show defects ranging from duplication of the wing pouch and hinge associated with a reduced thorax region to almost complete loss of the thorax region associated with a severely abnormal wing duplication. Large clones in the wing disc produce additional rows of sensory organ precursor cells, resulting in extra mechanosensory bristles in the anterior wing margin. These defects only occur when the clone affects both sides of the dorsoventral boundary. Some of the ectopic bristles are derived from wild-type cells neighbouring the clone.

(Giraldez et al., 2002)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Other

Statement

Reference

Notum³, Scer\GAL4^en-e16E, wg^UAS.cLa has ventral denticle belt phenotype

(Desbordes et al., 2005)

Notum³, Scer\GAL4^en-e16E, wg^UAS.cLa has embryonic/first instar larval cuticle phenotype

(Desbordes et al., 2005)

Notum³/Notum[+], Scer\GAL4^en-e16E, wg^UAS.cLa has ventral denticle belt phenotype

(Desbordes et al., 2005)

Notum³/Notum[+], Scer\GAL4^en-e16E, wg^UAS.cLa has embryonic/first instar larval cuticle phenotype

(Desbordes et al., 2005)

Notum³, dlp² has wing margin bristle | ectopic phenotype

(Kirkpatrick et al., 2004)

Notum³, dlp² has wing margin bristle | increased number phenotype

(Kirkpatrick et al., 2004)

Notum³/Notum[+], dlp² has wing margin bristle | ectopic phenotype

(Kirkpatrick et al., 2004)

Notum³/Notum[+], dlp² has wing margin bristle | increased number phenotype

(Kirkpatrick et al., 2004)

Additional Comments

Genetic Interactions

Statement

Reference

dlp² +/+ Notum³ transheterozygotes show increased bristle density at the anterior wing margin and ectopic bristles positioned further from the wing margin than in wild type. Neither dlp²/+ or Notum³/+ single mutant flies show this phenotype.

(Kirkpatrick et al., 2004)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Not³

(Desbordes et al., 2005)

Notum³

(Silver et al., 2007, Lim and Tomlinson, 2006)

Name Synonyms

Secondary FlyBase IDs

References (5)

Report Sections

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