FlyBase Allele Report: Zzzz\CAG[Q108.UAS.Tag:MYC,Tag:FLAG]

General Information

Symbol

Zzzz\CAG^{Q108.UAS.Tag:MYC,Tag:FLAG}

Species

a. artificial

Name

FlyBase ID

FBal0137335

Feature type

allele

Associated gene

Zzzz\CAG

Associated Insertion(s)

Carried in Construct

P{UAS-Q108.myc.flag}

Also Known As

UAS-Q108

Transgenic product class

trinucleotide_repeat_expansion

(Marsh et al., 2000)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

trinucleotide_repeat_expansion

(Marsh et al., 2000)

Mutagen

in vitro construct

(Kazantsev et al., 2002, Marsh et al., 2000)

Progenitor genotype

Carried in construct

P{UAS-Q108.myc.flag}

Cytology

Description

UAS regulatory sequences drive expression of a 108 amino acid polyglutamine repeat (the repeat is flanked by the amino acid sequence MRSRKL at the N-terminal end and by KLRS at the C-terminal end). The Tag:MYC and Tag:FLAG tags are present at the C-terminal end of the open reading frame (immediately after the KLRS sequence).

(Marsh et al., 2000)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

Tagged with

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of neurodegenerative disease

CEA

(Berger et al., 2006)

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

model of Huntington's disease

is ameliorated by Hsap\HTT^{Su3.Scer\UAS.T:Zzzz\CAG,T:Hsap\MYC}

(Kazantsev et al., 2002)

is ameliorated by Zzzz\CAG^{Su3.UAS.Tag:MYC}

(Kazantsev et al., 2002)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal eye color, with Scer\GAL4^GMR.PF

(Marsh et al., 2000)

partially lethal - majority die, with Scer\GAL4^elav-C155

(Berger et al., 2006, Berger et al., 2005)

partially lethal - majority die, with Scer\GAL4^GMR.PF

(Marsh et al., 2000)

partially lethal - majority die, with Scer\GAL4^hs.2sev

(Marsh et al., 2000)

short lived, with Scer\GAL4^elav.PU

(Marsh et al., 2000)

viable, with Scer\GAL4^dpp.blk1

(Marsh et al., 2000)

viable, with Scer\GAL4^elav.PU

(Marsh et al., 2000)

visible, with Scer\GAL4^dpp.blk1

(Marsh et al., 2000)

visible, with Scer\GAL4^GMR.PF

(Marsh et al., 2000)

visible, with Scer\GAL4^hs.2sev

(Marsh et al., 2000)

visible | adult stage, with Scer\GAL4^GMR.PF

(Iijima-Ando et al., 2010)

Phenotype Manifest In

adult thorax, with Scer\GAL4^dpp.blk1

(Marsh et al., 2000)

eye, with Scer\GAL4^GMR.PF

(Iijima-Ando et al., 2010, Marsh et al., 2000)

macrochaeta, with Scer\GAL4^hs.2sev

(Marsh et al., 2000)

retina, with Scer\GAL4^elav.PU

(Marsh et al., 2000)

rhabdomere, with Scer\GAL4^elav.PLu

(Kazantsev et al., 2002)

rhabdomere, with Scer\GAL4^elav.PU

(Marsh et al., 2000)

Detailed Description

Statement

Reference

Expression of Zzzz\CAG^{Q108.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG} in the eye under the control of Scer\GAL4^GMR.PF causes depigmentation.

(Iijima-Ando et al., 2010)

Expression of Zzzz\CAG^{Q108.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG} in neurons under the control of Scer\GAL4^elav-C155 causes dramatic pre-adult lethality, which is partially rescued by rapamycin treatment.

(Berger et al., 2006)

Expression of Zzzz\CAG^{Q108.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG} under the control of Scer\GAL4^elav-C155 results in dramatic pre-adult lethality, which is partially rescued by Li[+].

(Berger et al., 2005)

Expression of Zzzz\CAG^{Q108.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG} under the control of Scer\GAL4^elav.PLu results in degeneration of neurons in the eye, as indicated by large gaps in the eye tissue and loss of rhabdomeres. Each ommatidium contains on average only 3 neurons in these eyes (compared to 7 in wild type).

(Kazantsev et al., 2002)

Adult survivors expressing Zzzz\CAG^{Q108.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG} under the control of Scer\GAL4^dpp.blk1 often have a split thorax phenotype.

Adult survivors expressing Zzzz\CAG^{Q108.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG} under the control of Scer\GAL4^GMR.PF have a rough eye with no pigment.

Adult survivors expressing Zzzz\CAG^{Q108.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG} under the control of Scer\GAL4^hs.2sev show a loss of large sensory bristles.

External eye morphology is normal in adults expressing Zzzz\CAG^{Q108.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG} under the control of Scer\GAL4^elav.PU. However, many of the ommatidia contain only four to six rhabdomeres. Gaps are evident in the retinal tissue.

Adults expressing Zzzz\CAG^{Q108.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG} under the control of Scer\GAL4^elav.PU have a shortened lifespan compared to wild type.

(Marsh et al., 2000)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhanced by

Statement

Reference

Scer\GAL4^GMR.PF, Zzzz\CAG^{Q108.UAS.Tag:MYC,Tag:FLAG} has visible | adult stage phenotype, non-enhanceable by Scer\GAL4^GMR.PF/lok^UAS.cPa

(Iijima-Ando et al., 2010)

Phenotype Manifest In

NOT Enhanced by

Statement

Reference

Scer\GAL4^GMR.PF, Zzzz\CAG^{Q108.UAS.Tag:MYC,Tag:FLAG} has eye phenotype, non-enhanceable by Scer\GAL4^GMR.PF/lok^UAS.cPa

(Iijima-Ando et al., 2010)

Suppressed by

Statement

Reference

Scer\GAL4^elav.PLu, Zzzz\CAG^{Q108.UAS.Tag:MYC,Tag:FLAG} has rhabdomere phenotype, suppressible by Zzzz\CAG^{Su3.UAS.Tag:MYC}/Scer\GAL4^elav.PLu

(Kazantsev et al., 2002)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Expression of lok^Scer\UAS.cPa does not enhance the eye depigmentation phenotype seen when Zzzz\CAG^{Q108.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG} is expressed under the control of Scer\GAL4^GMR.PF.

(Iijima-Ando et al., 2010)

Co-expression of Hsap\HD^{Su3.Scer\UAS.T:Zzzz\CAG,T:Hsap\MYC} significantly suppresses the loss of photoreceptor neurons seen in flies expressing Zzzz\CAG^{Q108.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG} under the control of Scer\GAL4^elav.PLu.

(Kazantsev et al., 2002)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Zzzz\CAG^{Q108.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG}

Zzzz\CAG^{Q108.UAS.Tag:MYC,Tag:FLAG}

Name Synonyms

Secondary FlyBase IDs

References (5)

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