FB2024_03 , released June 25, 2024
Allele: Dmel\lokUAS.cPa
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General Information
Symbol
Dmel\lokUAS.cPa
Species
D. melanogaster
Name
Saccharomyces cerevisiae UAS construct a of Peters
FlyBase ID
FBal0147039
Feature type
allele
Associated gene
Dmel\lok
Associated Insertion(s)
Carried in Construct
P{UAS-lok.P}
Key Links
Transgenic product class
wild_type
(Peters et al., 2002)
Mutagen
Nature of the Allele
Transgenic product class
wild_type
(Peters et al., 2002)
Mutagen
in vitro construct
(Peters et al., 2002)
Progenitor genotype
Carried in construct
P{UAS-lok.P}
Cytology
Description

UAS regulatory sequences drive expression of lok.

(Peters et al., 2002)
Allele components
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
lok
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 1 )
      Disease
      Interaction
      References
      exacerbates  tauopathy
      modeled by Hsap\MAPTUAS.cWa
      (Iijima-Ando et al., 2010)
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Expression of lokScer\UAS.cPa under the control of Scer\GAL4GMR.PF does not cause obvious eye degeneration.

      (Iijima-Ando et al., 2010)

      Expression of lokScer\UAS.cPa under the control of Scer\GAL4GMR.PF does not result in an overt phenotype in the eye and no apoptotic cells are detected in third larval instar eye discs. Irradiated eye discs from larvae expressing lokScer\UAS.cPa under the control of Scer\GAL4GMR.PF posterior to the morphogenetic furrow show high numbers of apoptotic cells in this area (wild-type irradiated discs have an increased number of apoptotic cells both anterior and posterior to the furrow).

      (Peters et al., 2002)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Enhancer of
      Statement
      Reference

      Scer\GAL4GMR.PF/lokUAS.cPa is an enhancer of eye phenotype of Scer\GAL4GMR.PF, p53UAS.cPa

      (Peters et al., 2002)
      NOT Enhancer of
      NOT Suppressor of
      Statement
      Reference

      Scer\GAL4GMR.PF/lokUAS.cPa is a non-suppressor of eye phenotype of Scer\GAL4GMR.PF, p53S4A.UAS

      (Peters et al., 2002)
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Co-expression of lokScer\UAS.cPa and p53Scer\UAS.cPa under the control of Scer\GAL4GMR.PF results in almost complete loss of the eye. Excessive apoptosis is seen in third larval instar eye discs in these animals. The small eye phenotype caused by expression of p53S4A.Scer\UAS under the control of Scer\GAL4GMR.PF is not altered by coexpression of lokScer\UAS.cPa.

      (Peters et al., 2002)
      Xenogenetic Interactions
      Statement
      Reference

      Expression of lokScer\UAS.cPa does not enhance the eye depigmentation phenotype seen when Zzzz\CAGQ108.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG is expressed under the control of Scer\GAL4GMR.PF.

      Expression of lokScer\UAS.cPa does not enhance the eye degeneration phenotype seen when Hsap\APPAβ1-42.Scer\UAS.Arc is expressed under the control of Scer\GAL4GMR.PF.

      Co-expression of Hsap\MAPTScer\UAS.S262A and lokScer\UAS.cPa under the control of Scer\GAL4GMR.PF has no effect on external eye size.

      Expression of lokScer\UAS.cPa does not enhance the eye degeneration phenotype seen when par-1Scer\UAS.T:Hsap\MYC is expressed under the control of Scer\GAL4GMR.PF.

      (Iijima-Ando et al., 2010)
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      Comments
      Comments

      Carried in plasmid "pUAST-dm-chk2-wt", transfected into S2 cells with a driver construct and used in transcription activation assays.

      (Peters et al., 2002)
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      lokScer\UAS.cPa
      lokUAS.cPa
      Name Synonyms
      Saccharomyces cerevisiae UAS construct a of Peters
      (Peters et al., 2002)
      Secondary FlyBase IDs
        References (2)