UAS regulatory sequences drive expression of a 108 amino acid polyglutamine repeat (the repeat is flanked by the amino acid sequence MRSRKL at the N-terminal end and by KLRS at the C-terminal end). The Tag:MYC and Tag:FLAG tags are present at the C-terminal end of the open reading frame (immediately after the KLRS sequence).
Expression of Zzzz\CAGQ108.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG in the eye under the control of Scer\GAL4GMR.PF causes depigmentation.
Expression of Zzzz\CAGQ108.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG in neurons under the control of Scer\GAL4elav-C155 causes dramatic pre-adult lethality, which is partially rescued by rapamycin treatment.
Expression of Zzzz\CAGQ108.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG under the control of Scer\GAL4elav-C155 results in dramatic pre-adult lethality, which is partially rescued by Li[+].
Expression of Zzzz\CAGQ108.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG under the control of Scer\GAL4elav.PLu results in degeneration of neurons in the eye, as indicated by large gaps in the eye tissue and loss of rhabdomeres. Each ommatidium contains on average only 3 neurons in these eyes (compared to 7 in wild type).
Adult survivors expressing Zzzz\CAGQ108.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG under the control of Scer\GAL4dpp.blk1 often have a split thorax phenotype.
Adult survivors expressing Zzzz\CAGQ108.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG under the control of Scer\GAL4GMR.PF have a rough eye with no pigment.
Adult survivors expressing Zzzz\CAGQ108.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG under the control of Scer\GAL4hs.2sev show a loss of large sensory bristles.
External eye morphology is normal in adults expressing Zzzz\CAGQ108.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG under the control of Scer\GAL4elav.PU. However, many of the ommatidia contain only four to six rhabdomeres. Gaps are evident in the retinal tissue.
Adults expressing Zzzz\CAGQ108.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG under the control of Scer\GAL4elav.PU have a shortened lifespan compared to wild type.
Scer\GAL4GMR.PF, Zzzz\CAGQ108.UAS.Tag:MYC,Tag:FLAG has visible | adult stage phenotype, non-enhanceable by Scer\GAL4GMR.PF/lokUAS.cPa
Scer\GAL4GMR.PF, Zzzz\CAGQ108.UAS.Tag:MYC,Tag:FLAG has eye phenotype, non-enhanceable by Scer\GAL4GMR.PF/lokUAS.cPa
Scer\GAL4elav.PLu, Zzzz\CAGQ108.UAS.Tag:MYC,Tag:FLAG has rhabdomere phenotype, suppressible by Zzzz\CAGSu3.UAS.Tag:MYC/Scer\GAL4elav.PLu
Expression of lokScer\UAS.cPa does not enhance the eye depigmentation phenotype seen when Zzzz\CAGQ108.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG is expressed under the control of Scer\GAL4GMR.PF.
Co-expression of Hsap\HDSu3.Scer\UAS.T:Zzzz\CAG,T:Hsap\MYC significantly suppresses the loss of photoreceptor neurons seen in flies expressing Zzzz\CAGQ108.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG under the control of Scer\GAL4elav.PLu.