FlyBase Allele Report: Dmel\rn[UAS.cSPa]

General Information

Symbol

Dmel\rn^UAS.cSPa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of St Pierre

FlyBase ID

FBal0137098

Feature type

allele

Associated gene

Dmel\rn

Associated Insertion(s)

Carried in Construct

P{UAS-rn.SP}

Also Known As

UAS-rn, UASrn

Key Links

Transgenic product class

genomic_DNA

(St Pierre et al., 2002)

wild_type

(St Pierre et al., 2002)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

genomic_DNA

(St Pierre et al., 2002)

wild_type

(St Pierre et al., 2002)

Mutagen

in vitro construct

(St Pierre et al., 2002)

Progenitor genotype

Carried in construct

P{UAS-rn.SP}

Cytology

Description

UASt regulatory sequences drive expression of the "rn" isoform of rn (nucleotides 0-3373 of AF395905 and 86bp of upstream genomic DNA).

(St Pierre et al., 2002)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy

(Li et al., 2013)

visible, with Scer\GAL4^GMR.PF

(St Pierre et al., 2002)

Phenotype Manifest In

antennal intermediate sensillum ai3 | increased number

(Li et al., 2013)

antennal trichoid sensillum at1

(Li et al., 2013)

antennal trichoid sensillum at4

(Li et al., 2013)

eye, with Scer\GAL4^GMR.PF

(St Pierre et al., 2002)

Detailed Description

Statement

Reference

Expression of rn^{Scer\UAS.cSPa} in the trichoid and basiconic lineage precursors under the control of Scer\GAL4^amos.PZ leads to an increase in rn-positive at3 sensilla olfactory receptor neurons (ORNs) and a decreases in the rn-positive at4 ORNs. There is also a decrease in the rn-positive at1 ORNs.

(Li et al., 2013)

Expression of rn^{Scer\UAS.cSPa} under the control of Scer\GAL4^rn-GAL4-5 has no obvious effect on the leg. Expression of rn^{Scer\UAS.cSPa} under the control of Scer\GAL4^GMR.PF disrupts the morphology and size of the adult eye. Expression of rn^{Scer\UAS.cSPa} under the control of Scer\GAL4^sev.EP results in little if any rough eye morphology.

(St Pierre et al., 2002)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Suppressor of

Statement

Reference

rn^UAS.cSPa/Scer\GAL4^Bx-MS1096 is a non-suppressor of visible phenotype of Scer\GAL4^Bx-MS1096, hng1^KK103642

(Shukla et al., 2014)

Other

Statement

Reference

Scer\GAL4^dpp.blk1, nab^GD1607, rn^UAS.cSPa has visible phenotype

(Perea et al., 2013)

Phenotype Manifest In

Suppressor of

Statement

Reference

rn^UAS.cSPa is a suppressor | partially of wing hinge phenotype of Scer\GAL4^rn-GAL4-13, nab^EP13

(Félix et al., 2007)

rn^UAS.cSPa is a suppressor | partially of tarsal segment 2 phenotype of Scer\GAL4^rn-GAL4-13, nab^EP13

(Félix et al., 2007)

rn^UAS.cSPa is a suppressor | partially of tarsal segment 3 phenotype of Scer\GAL4^rn-GAL4-13, nab^EP13

(Félix et al., 2007)

rn^UAS.cSPa is a suppressor | partially of tarsal segment 4 phenotype of Scer\GAL4^rn-GAL4-13, nab^EP13

(Félix et al., 2007)

NOT Suppressor of

Statement

Reference

rn^UAS.cSPa/Scer\GAL4^Bx-MS1096 is a non-suppressor of wing hinge phenotype of Scer\GAL4^Bx-MS1096, hng1^KK103642

(Shukla et al., 2014)

Other

Statement

Reference

Scer\GAL4^dpp.blk1, nab^GD1607, rn^UAS.cSPa has wing pouch | ectopic phenotype

(Perea et al., 2013)

Scer\GAL4^dpp.blk1, nab^GD1607, rn^UAS.cSPa has mesothoracic tergum primordium phenotype

(Perea et al., 2013)

Additional Comments

Genetic Interactions

Statement

Reference

Expressing rn^{Scer\UAS.cSPa} has no effect on the wing hinge phenotype seen when hng1^KK103642 is expressed under the control of Scer\GAL4^Bx-MS1096.

(Shukla et al., 2014)

Co-expression of rn^{Scer\UAS.cSPa} and nab^GD1607 under the control of Scer\GAL4^dpp.blk1 results in the formation of an extra wing pouch in the developing notum of the wing disc.

(Perea et al., 2013)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

rn^UAS.cSPa/Scer\GAL4^rn-GAL4-5 rescues Df(3R)rn20/rn^GAL4-5

(St Pierre et al., 2002)

Fails to rescue

Scer\GAL4^sev.EP/rn^UAS.cSPa fails to rescue Df(3R)rn20/rn¹⁶

(St Pierre et al., 2002)

Comments