FB2024_03 , released June 25, 2024
Allele: Dmel\unc-5UAS.Tag:HA,Tag:SS(wg)
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General Information
Symbol
Dmel\unc-5UAS.Tag:HA,Tag:SS(wg)
Species
D. melanogaster
Name
FlyBase ID
FBal0128974
Feature type
allele
Associated gene
Dmel\unc-5
Associated Insertion(s)
Carried in Construct
P{UAS-unc-5::wg.HA1}
Also Known As
UAS-HA-Unc5, UAS-Unc5
Key Links
Transgenic product class
Mutagen
Nature of the Allele
Transgenic product class
Mutagen
in vitro construct
(Keleman and Dickson, 2001)
Progenitor genotype
Carried in construct
P{UAS-unc-5::wg.HA1}
Cytology
Description

Expression of a coding region made up of amino acids 1-32 of wg (including the signal sequence) fused to AR fused to three Tag:HA tags fused to SLD fused to amino acids 32-536 of unc-5 (extracellular and transmembrane domain) fused to SF fused to amino acids 536-1072 of unc-5 (cytoplasmic domain) is governed by UAS regulatory sequences.

(Keleman and Dickson, 2001)
Allele components
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
unc-5
Tagged with
Tag:HA
Tag:SS(wg)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      EW neurons misexpressing unc-5Scer\UAS.T:Ivir\HA1,T:SS-wg, under the control of Scer\GAL4eg-Mz360, do not cross the midline but fail to project laterally.

      (Zarin et al., 2014)

      Embryos expressing unc-5Scer\UAS.T:Ivir\HA1,T:SS-wg under the control of Scer\GAL4eve.RN2 do not have guidance defects in the intersegmental nerve.

      (Zarin et al., 2012)

      Expression of unc-5Scer\UAS.T:Ivir\HA1,T:SS-wg under the control of Scer\GAL4repo has no effect on the migration of embryonic peripheral glia. However, ectopic interface glia are frequently seen in the periphery.

      (von Hilchen et al., 2010)

      Misexpression of unc-5Scer\UAS.T:Ivir\HA1,T:wg, under the control of Scer\GAL4elav.PLu, results in the redirection of the ventral-ISNb branch, which joins the dorsal ISN and projects dorsally. The lateral SNa is not affected.

      (Labrador et al., 2005)

      When unc-5Scer\UAS.T:Ivir\HA1,T:wg is driven by Scer\GAL4ap-md544, ap expressing neurons are driven laterally to exit the ventral nerve cord into the periphery.

      (Allan et al., 2003)

      When expression is driven by Scer\GAL4elav.PLu commissures are completely lacking from the embryonic CNS, because the commissural axons are prevented from crossing the midline. When expression is driven by Scer\GAL4ap-md544, the axon in which unc-5Scer\UAS.T:Ivir\HA1,T:wg is expressed projects directly away from the midline, as opposed to the wild type situation of it projecting towards the midline.

      (Keleman and Dickson, 2001)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Enhanced by
      Suppressed by
      NOT suppressed by
      Enhancer of
      Suppressor of
      Other
      Phenotype Manifest In
      Enhanced by
      Suppressed by
      NOT suppressed by
      Enhancer of
      Suppressor of
      Other
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Expression of unc-5Scer\UAS.T:Ivir\HA1,T:SS-wg in a Df(2R)eve mutant background, under the control of Scer\GAL4eve.RN2 leads to both aCC and RP2 motor neurons displaying an almost exclusively single exit from the CNS.

      Expression of unc-5Scer\UAS.T:Ivir\HA1,T:SS-wg in an eveΔRP2A mutant background results in 11% of hemisegments exhibiting dual motoneuron exit. These mutants exhibit an increase in dorsal projections in 67% of hemisegments.

      Co-expression of beat-IaScer\UAS.cFa and unc-5Scer\UAS.T:Ivir\HA1,T:SS-wg in a Df(2R)eve mutant background, under the control of Scer\GAL4eve.RN2 leads to a more robust CNS exit for motor neurons, with many hemisegments displaying exit of both motor neurons. There are two types of exit: unfasciculated, both axons from the same hemisegment chose a different nerve root, or fasciculated, where axons join and exit through the intersegmental nerve root.

      Co-expression of unc-5Scer\UAS.T:Ivir\HA1,T:SS-wg and Nrg180.I.Scer\UAS in a Df(2R)eve mutant background, under the control of Scer\GAL4eve.RN2 leads to a more robust CNS exit for motor neurons, with many hemisegments displaying exit of both motor neurons. There are two types of exit: unfasciculated, both axons from the same hemisegment chose a different nerve root, or fasciculated, where axons join and exit through the intersegmental nerve root.

      Co-expression of unc-5Scer\UAS.T:Ivir\HA1,T:SS-wg and Fas2Scer\UAS.cLa in a Df(2R)eve mutant background, under the control of Scer\GAL4eve.RN2 leads to a more robust CNS exit for motor neurons, with many hemisegments displaying exit of both motor neurons. There are two types of exit: unfasciculated, both axons from the same hemisegment chose a different nerve root, or fasciculated, where axons join and exit through the intersegmental nerve root.

      Co-expression of unc-5Scer\UAS.T:Ivir\HA1,T:SS-wg, beat-IaScer\UAS.cFa and Nrg180.I.Scer\UAS in a Df(2R)eve mutant background, under the control of Scer\GAL4eve.RN2 leads to a more robust CNS exit for motor neurons, with many hemisegments displaying exit of both motor neurons. The number of hemisegments with one neuronal exit is reduced, compared to controls and single and double mutants.

      Co-expression of unc-5Scer\UAS.T:Ivir\HA1,T:SS-wg, beat-IaScer\UAS.cFa and Fas2Scer\UAS.cLa in a Df(2R)eve mutant background, under the control of Scer\GAL4eve.RN2 leads to a more robust CNS exit for motor neurons, with many hemisegments displaying exit of both motor neurons. The number of hemisegments with one neuronal exit is reduced, compared to controls and single and double mutants.

      Combinatorial misexpression of unc-5Scer\UAS.T:Ivir\HA1,T:SS-wg with Nrg180.I.Scer\UAS under the control of Scer\GAL4eg-Mz360, results in lateral exit of 8% of EW axons.

      Triple misexpression of unc-5Scer\UAS.T:Ivir\HA1,T:SS-wg, beat-IaScer\UAS.cFa, and Nrg180.I.Scer\UAS in EW neurons, under the control of Scer\GAL4eg-Mz360, leads to lateral redirection of axons in 20% of hemisegments.

      Quadrupal misexpression of unc-5Scer\UAS.T:Ivir\HA1,T:SS-wg, beat-IaScer\UAS.cFa, Nrg180.I.Scer\UAS and Fas2Scer\UAS.cLa in EW neurons, under the control of Scer\GAL4eg-Mz360, leads to lateral redirection of axons in 33% of hemisegments.

      (Zarin et al., 2014)

      The defects in intersegmental nerve innervation of muscles 1/9 are partially rescued by expression of unc-5Scer\UAS.T:Ivir\HA1,T:SS-wg under the control of Scer\GAL4eve.RN2.

      (Zarin et al., 2012)

      The ventral-ISNb branch projection phenotype is suppressed when unc-5Scer\UAS.T:Ivir\HA1,T:wg is expressed, under the control of Scer\GAL4elav.PLu, in a NetA, NetB double mutant background.

      (Labrador et al., 2005)

      When in combination with sli2/sli2 the interaction is difficult to interpret - collapse of longitudinal fibers onto the midline is hindered, but midline cells are still displaced.

      (Keleman and Dickson, 2001)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescues

      unc-5UAS.Tag:HA,Tag:SS(wg)/Scer\GAL4Mef2.247 rescues unc-58

      (Raza and Jacobs, 2016)
      Partially rescues

      unc-5UAS.Tag:HA,Tag:SS(wg)/Scer\GAL4cas-3921 partially rescues unc-58

      (von Hilchen et al., 2010)

      unc-5UAS.Tag:HA,Tag:SS(wg)/Scer\GAL4repo partially rescues unc-58

      (von Hilchen et al., 2010)

      unc-5UAS.Tag:HA,Tag:SS(wg)/Scer\GAL4elav.PLu partially rescues unc-58/unc-52

      (Labrador, 2005)
      Comments

      The expression of unc-5Scer\UAS.T:Ivir\HA1,T:SS-wg under the control of Scer\GAL4Mef2.247 rescues the unc-58 homozygous embryonic heart cardioblast defects (i.e. migration velocity and in filopodial and lamellopodial extensions and activities) and embryonic heart lumen formation defects.

      (Raza and Jacobs, 2016)

      Expression of unc-5Scer\UAS.T:Ivir\HA1,T:SS-wg under the control of Scer\GAL4cas-3921 in unc-58 embryos rescues the normal number of six embryonic peripheral glia distal to the SNc in 49% of hemisegments (compared to only 6% of hemisegments in unc-58 embryos) and five ePG are found distal to the SNc in 39% of hemisegments (compared to only 24% of hemisegments in unc-58 embryos).

      Expression of unc-5Scer\UAS.T:Ivir\HA1,T:SS-wg under the control of Scer\GAL4repo in unc-58 embryos rescues the normal number of six embryonic peripheral glia distal to the SNc in 49% of hemisegments (compared to only 6% of hemisegments in unc-58 embryos) and five ePG are found distal to the SNc in 33% of hemisegments (compared to only 24% of hemisegments in unc-58 embryos).

      (von Hilchen et al., 2010)

      Expression of unc-5Scer\UAS.T:Ivir\HA1,T:wg, under the control of Scer\GAL4elav.PLu, partially rescues the ISN and SNa defects of unc-52/unc-58 mutants.

      (Labrador, 2005)
      Images (0)
      Mutant
      Wild-type
      Stocks (2)
      Notes on Origin
      Discoverer
      Comments
      Comments

      Four out of four inserts tested gave completely penetrant phenotype with single copy of construct.

      (Keleman and Dickson, 2001)
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      unc-5Scer\UAS.T:Ivir\HA1,T:SS-wg
      unc-5Scer\UAS.T:Ivir\HA1,T:wg
      unc-5UAS.Tag:HA,Tag:SS(wg)
      Name Synonyms
      Secondary FlyBase IDs
        References (11)