eye, with Scer\GAL4GMR.PF
sensory mother cell & dorsal mesothoracic disc, with Scer\GAL4Bx-MS1096
Expression of ttk69.Scer\UAS under the control of Scer\GAL4GMR.PF causes a distorted eye morphology characterized by disorganised facets and a loss of bristles. The cone cell pattern in the ommatidium of 40hr old pupal eye discs is also severely disrupted. The photoreceptor pattern is severely disrupted.
When ttk69.Scer\UAS is driven by Scer\GAL4Bx-MS1096 the wing blade area is reduced and all sensilla are removed except the ventral mechanosensory bristles. Sensory organ precursors are ablated in the wing disc.
Expression of ttk69.Scer\UAS under the control of Scer\GAL4GMR.PF abolishes the second mitotic wave in the eye disc. The proportion of cells behind the furrow in G2 is increased in these discs compared to wild type.
Expression of ttk69.Scer\UAS under the control of Scer\GAL4elav-C155 results in pathfinding and fasciculation defects in the central nervous system (CNS). The longitudinal fascicles are disorganised and the intersegmental nerve is misrouted and often fails to exit the CNS. Organisation of the peripheral nervous system is unaffected. Expression of ttk69.Scer\UAS under the control of Scer\GAL4Kr.PM inhibits normal glial development. Longitudinal glia are ablated when ttk69.Scer\UAS is expressed under the control of Scer\GAL4sca-537.4. The number of longitudinal glia is reduced when ttk69.Scer\UAS is expressed under the control of Scer\GAL4MZ1580. The normal BrdU incorporation in the ventral neuroectoderm is inhibited in embryos expressing ttk69.Scer\UAS under the control of Scer\GAL4Kr.PM.
Scer\GAL4GMR.PF, ttk69.UAS has visible phenotype, enhanceable by Usp47UAS.cBa/Scer\GAL4GMR.PF
Scer\GAL4GMR.PF, ttk69.UAS has visible phenotype, non-enhanceable by Usp47C405A.UAS/Scer\GAL4GMR.PF
Scer\GAL4GMR.PF, ttk69.UAS has visible phenotype, suppressible | partially by Ubp64E[+]/Usp47Δ1
Scer\GAL4GMR.PF, ttk69.UAS has visible phenotype, non-suppressible by Ubp64E[+]/Usp47Rev
ttk69.UAS/Scer\GAL4GMR.PF is an enhancer of visible phenotype of Scer\GAL4GMR.PF, Usp47UAS.cBa
Scer\GAL4GMR.PF, ttk69.UAS has interommatidial bristle phenotype, enhanceable by Usp47UAS.cBa/Scer\GAL4GMR.PF
Scer\GAL4GMR.PF, ttk69.UAS has eye disc | pupal stage phenotype, enhanceable by Usp47UAS.cBa/Scer\GAL4GMR.PF
Scer\GAL4GMR.PF, ttk69.UAS has ommatidium | pupal stage phenotype, enhanceable by Usp47UAS.cBa/Scer\GAL4GMR.PF
Scer\GAL4GMR.PF, ttk69.UAS has photoreceptor neuron | pupal stage phenotype, enhanceable by Usp47UAS.cBa/Scer\GAL4GMR.PF
ttk69.UAS has cone cell | pupal stage phenotype, enhanceable by Usp47UAS.cBa/Scer\GAL4GMR.PF
Scer\GAL4GMR.PF, ttk69.UAS has eye phenotype, enhanceable by Usp47UAS.cBa/Scer\GAL4GMR.PF
Scer\GAL4GMR.PF, ttk69.UAS has ommatidium | adult stage phenotype, enhanceable by Usp47UAS.cBa/Scer\GAL4GMR.PF
Scer\GAL4GMR.PF, ttk69.UAS has eye phenotype, non-enhanceable by Usp47C405A.UAS/Scer\GAL4GMR.PF
Scer\GAL4GMR.PF, ttk69.UAS has ommatidium | adult stage phenotype, non-enhanceable by Usp47C405A.UAS/Scer\GAL4GMR.PF
Scer\GAL4GMR.PF, ttk69.UAS has interommatidial bristle phenotype, non-enhanceable by Usp47C405A.UAS/Scer\GAL4GMR.PF
Scer\GAL4GMR.PF, ttk69.UAS has eye disc | pupal stage phenotype, non-enhanceable by Usp47C405A.UAS/Scer\GAL4GMR.PF
Scer\GAL4GMR.PF, ttk69.UAS has ommatidium | pupal stage phenotype, non-enhanceable by Usp47C405A.UAS/Scer\GAL4GMR.PF
Scer\GAL4GMR.PF, ttk69.UAS has cone cell | pupal stage phenotype, non-enhanceable by Usp47C405A.UAS/Scer\GAL4GMR.PF
Scer\GAL4GMR.PF, ttk69.UAS has photoreceptor neuron | pupal stage phenotype, non-enhanceable by Usp47C405A.UAS/Scer\GAL4GMR.PF
Scer\GAL4GMR.PF, ttk69.UAS has eye phenotype, suppressible | partially by Ubp64E[+]/Usp47Δ1
Scer\GAL4GMR.PF, ttk69.UAS has ommatidium | adult stage phenotype, suppressible | partially by Ubp64E[+]/Usp47Δ1
Scer\GAL4GMR.PF, ttk69.UAS has interommatidial bristle phenotype, suppressible | partially by Ubp64E[+]/Usp47Δ1
Scer\GAL4GMR.PF, ttk69.UAS has eye disc | pupal stage phenotype, suppressible | partially by Ubp64E[+]/Usp47Δ1
Scer\GAL4GMR.PF, ttk69.UAS has ommatidium | pupal stage phenotype, suppressible | partially by Ubp64E[+]/Usp47Δ1
Scer\GAL4GMR.PF, ttk69.UAS has cone cell | pupal stage phenotype, suppressible | partially by Ubp64E[+]/Usp47Δ1
Scer\GAL4GMR.PF, ttk69.UAS has photoreceptor neuron | pupal stage phenotype, suppressible | partially by Ubp64E[+]/Usp47Δ1
Scer\GAL4GMR.PF, ttk69.UAS has mitotic cell cycle phenotype, suppressible by stgUAS.cNa/Scer\GAL4GMR.PF
Scer\GAL4GMR.PF, ttk69.UAS has eye disc phenotype, suppressible by stgUAS.cNa/Scer\GAL4GMR.PF
Scer\GAL4GMR.PF, ttk69.UAS has eye phenotype, non-suppressible by Ubp64E[+]/Usp47Rev
Scer\GAL4GMR.PF, ttk69.UAS has ommatidium | adult stage phenotype, non-suppressible by Ubp64E[+]/Usp47Rev
Scer\GAL4GMR.PF, ttk69.UAS has interommatidial bristle phenotype, non-suppressible by Ubp64E[+]/Usp47Rev
Scer\GAL4GMR.PF, ttk69.UAS has eye disc | pupal stage phenotype, non-suppressible by Ubp64E[+]/Usp47Rev
Scer\GAL4GMR.PF, ttk69.UAS has ommatidium | pupal stage phenotype, non-suppressible by Ubp64E[+]/Usp47Rev
Scer\GAL4GMR.PF, ttk69.UAS has cone cell | pupal stage phenotype, non-suppressible by Ubp64E[+]/Usp47Rev
Scer\GAL4GMR.PF, ttk69.UAS has photoreceptor neuron | pupal stage phenotype, non-suppressible by Ubp64E[+]/Usp47Rev
ttk69.UAS/Scer\GAL4GMR.PF is an enhancer of eye phenotype of Scer\GAL4GMR.PF, Usp47UAS.cBa
ttk69.UAS/Scer\GAL4GMR.PF is an enhancer of ommatidium | adult stage phenotype of Scer\GAL4GMR.PF, Usp47UAS.cBa
ttk69.UAS/Scer\GAL4GMR.PF is an enhancer of interommatidial bristle phenotype of Scer\GAL4GMR.PF, Usp47UAS.cBa
ttk69.UAS/Scer\GAL4GMR.PF is an enhancer of eye disc | pupal stage phenotype of Scer\GAL4GMR.PF, Usp47UAS.cBa
ttk69.UAS/Scer\GAL4GMR.PF is an enhancer of ommatidium | pupal stage phenotype of Scer\GAL4GMR.PF, Usp47UAS.cBa
ttk69.UAS/Scer\GAL4GMR.PF is an enhancer of photoreceptor neuron | pupal stage phenotype of Scer\GAL4GMR.PF, Usp47UAS.cBa
ttk69.UAS/Scer\GAL4GMR.PF is an enhancer of cone cell | pupal stage phenotype of Scer\GAL4GMR.PF, Usp47UAS.cBa
One copy of Ubp64EΔ1 strongly suppresses the adult eye morphology phenotype seen when ttk69.Scer\UAS is expressed under the control of Scer\GAL4GMR.PF. The cone cell disruption see in pupal eye discs is also suppressed, as is the disruption to the photoreceptor pattern.
One copy of Ubp64ERev is unable to suppress the adult eye morphology phenotype seen when ttk69.Scer\UAS is expressed under the control of Scer\GAL4GMR.PF. The cone cell pattern disruption and loss of photoreceptors seen in pupal ommatidia is also unaffected.
Co-expression of ttk69.Scer\UAS and Ubp64EScer\UAS.cBa under the control of Scer\GAL4GMR.PF causes dramatically defective eye development, more severe than is seen when either transgene is expressed alone. The cone cell disruption seen in the ommatidia of pupal eye discs is also enhanced, as is the disruption to the photoreceptor pattern.
Expression of Ubp64EC405A.Scer\UAS does not enhance the adult eye phenotypes seen when ttk69.Scer\UAS is expressed under the control of Scer\GAL4GMR.PF. The cone cell and photoreceptor disruption seen in the ommatidia of pupal eye discs is also unaffected.
Coexpression of stgScer\UAS.cNa completely rescues the second mitotic wave in eye discs expressing ttk69.Scer\UAS under the control of Scer\GAL4GMR.PF.