FlyBase Allele Report: Dmel\pygo[EP1076]

General Information

Symbol

Dmel\pygo^EP1076

Species

D. melanogaster

Name

FlyBase ID

FBal0119700

Feature type

allele

Associated gene

Dmel\pygo

Associated Insertion(s)

P{EP}pygo^EP1076

Carried in Construct

Also Known As

EP(3)1076

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

P-element activity

Nature of the Allele

Allele class

Mutagen

P-element activity

(Belenkaya et al., 2002, Parker et al., 2002, Thompson et al., 2002, Tseng and Hariharan, 2002, Gene Disruption Project members, 2001-, Abdelilah-Seyfried et al., 2000)

Progenitor genotype

Associated Insertion(s)

P{EP}pygo^EP1076

Cytology

Description

Insertion of a P{EP} element 500bp upstream of the putative start codon of the pygo gene prediction CG11518.

(Belenkaya et al., 2002)

P{EP} insertion within the first intron of pygo.

(Thompson et al., 2002)

P{EP} insertion in the 5' UTR of pygos.

(Tseng and Hariharan, 2002)

P{EP} insertion within the transcription unit.

(Abdelilah-Seyfried et al., 2000)

Allele components

Component

Use(s)

Inserted element

P{EP}

misexpression element

Regulatory region(s)

UAS

binary expression system - regulatory region

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

3R:31,579,900..31,579,900 [-]

(Belenkaya et al., 2002, Parker et al., 2002, Thompson et al., 2002, Tseng and Hariharan, 2002, Gene Disruption Project members, 2001-, Abdelilah-Seyfried et al., 2000)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal - all die before end of pupal stage

(Parker et al., 2002)

some die during pupal stage

(Parker et al., 2002)

visible, with Scer\GAL4^en-e16E

(Tseng and Hariharan, 2002)

visible, with Scer\GAL4^ey.PH

(Tseng and Hariharan, 2002)

visible, with Scer\GAL4^sca-537.4

(Abdelilah-Seyfried et al., 2000)

Phenotype Manifest In

adult cuticle

(Parker et al., 2002)

dorsocentral bristle, with Scer\GAL4^sca-537.4

(Abdelilah-Seyfried et al., 2000)

eye, with Scer\GAL4^ey.PH

(Tseng and Hariharan, 2002)

leg

(Parker et al., 2002)

scutellar bristle, with Scer\GAL4^sca-537.4

(Abdelilah-Seyfried et al., 2000)

sex comb

(Parker et al., 2002)

wing, with Scer\GAL4^en-e16E

(Tseng and Hariharan, 2002)

Detailed Description

Statement

Reference

pygo^EP1076 homozygotes (not driven by Scer\GAL4) are late pupal lethal and exhibit several defects in their exoskeleton including malformed legs. The sex comb is also missing.

(Parker et al., 2002)

Expression of pygo^EP1076 under the control of Scer\GAL4^ey.PH results in eyes that are somewhat reduced in size. Flies expressing pygo^EP1076 under the control of Scer\GAL4^hs.2sev have wild-type eyes. Flies expressing pygo^EP1076 under the control of Scer\GAL4^en-e16E have reduced wings. Cells from third larval instar wing discs expressing pygo^EP1076 under the control of Scer\GAL4^Act5C.PP are normal in size. There is a small increase in the population of cells with a 4N DNA content.

(Tseng and Hariharan, 2002)

Expression of pygo^EP1076 under the control of Scer\GAL4^sca-537.4 results in the loss or ectopic positioning of a few scutellar and dorsocentral macrochaetae.

(Abdelilah-Seyfried et al., 2000)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhanced by

Statement

Reference

Scer\GAL4^sca-537.4, pygo^EP1076 has visible phenotype, non-enhanceable by H[+]/H^E21

(Abdelilah-Seyfried et al., 2000)

Suppressed by

Statement

Reference

Scer\GAL4^ey.PH, pygo^EP1076 has visible phenotype, suppressible by CycE^UAS.cLa, Scer\GAL4^ey.PH

(Tseng and Hariharan, 2002)

NOT suppressed by

Statement

Reference

Scer\GAL4^sca-537.4, pygo^EP1076 has visible phenotype, non-suppressible by H[+]/H^E21

(Abdelilah-Seyfried et al., 2000)

Phenotype Manifest In

Suppressed by

Statement

Reference

Scer\GAL4^ey.PH, pygo^EP1076 has eye phenotype, suppressible by CycE^UAS.cLa, Scer\GAL4^ey.PH

(Tseng and Hariharan, 2002)

NOT Enhancer of

Statement

Reference

pygo^EP1076/Scer\GAL4^sca-537.4 is a non-enhancer of phenotype of H^E21

(Abdelilah-Seyfried et al., 2000)

Suppressor of

Statement

Reference

pygo^EP1076/Scer\GAL4^GMR.PF is a suppressor of eye phenotype of Scer\GAL4^GMR.PF, wg^UAS.cKa

(Parker et al., 2002)

pygo^EP1076/Scer\GAL4^GMR.PF is a suppressor of eye phenotype of arm^S56F.GMR

(Parker et al., 2002)

pygo^EP1076/Scer\GAL4^GMR.PF is a suppressor of interommatidial bristle phenotype of wg^sev.PC

(Parker et al., 2002)

pygo[+]/pygo^EP1076 is a suppressor of phenotype of arm^S56F.GMR

(Thompson et al., 2002)

NOT Suppressor of

Statement

Reference

pygo^EP1076/Scer\GAL4^sca-537.4 is a non-suppressor of phenotype of H^E21

(Abdelilah-Seyfried et al., 2000)

Additional Comments

Genetic Interactions

Statement

Reference

Co-expression of CycE^Scer\UAS.cLa suppresses the small eye phenotype caused by expression of pygo^EP1076 under the control of Scer\GAL4^ey.PH. Cells from third larval instar wing discs expressing pygo^EP1076 under the control of Scer\GAL4^Act5C.PP (and also co-expressing BacA\p35^Scer\UAS.cHa to eliminate cell death) have a 10% longer doubling time than control cells expressing BacA\p35^Scer\UAS.cHa alone under the control of Scer\GAL4^Act5C.PP.

(Tseng and Hariharan, 2002)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to complement

pygo^F107

(Belenkaya et al., 2002)

pygo^F15

(Belenkaya et al., 2002)

pygo^F66

(Belenkaya et al., 2002)

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (5)

Reported As

Symbol Synonym

BEST:LD21971^EP1076

EP(3)1076

(Parker et al., 2002, Abdelilah-Seyfried et al., 2000)

gam^EP1076

pygo^EP1076

pygo^EP

(Parker et al., 2002)

Name Synonyms

Secondary FlyBase IDs

References (7)

Report Sections

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