Ptp10D101/Ptp10D1, Ptp69D4 has larval ventral nerve cord commissure phenotype
Ptp10D101/Ptp10D1, Ptp69Dunspecified has larval abdominal segmental nerve phenotype
Ptp10D101/Ptp10D1, Ptp69Dunspecified has larval RP5 motor neuron phenotype
Ptp10D101, Ptp69D1/Ptp69D8ex25 has larval longitudinal connective phenotype
Ptp10D101, Ptp69Dunspecified has larval VL3/4 motor neuron phenotype
Ptp10D101, Ptp69Dunspecified has larval RP2 motor neuron phenotype
Ptp10D101, Ptp69Dunspecified has larval intersegmental nerve phenotype
Ptp10D101, Ptp69Dunspecified has larval VO2 motor neuron phenotype
Ptp10D101, Ptp69Dunspecified has larval VO1 motor neuron phenotype
Ptp10D101, Ptp69Dunspecified has larval abdominal segmental nerve phenotype
Ptp10D101, Ptp69Dunspecified has larval RP5 motor neuron phenotype
Ptp10D101, Ptp69D8ex25/Ptp69D4 has larval ventral nerve cord commissure phenotype
Ptp10D101/Ptp10D1, Ptp69D8ex25 has larval ventral nerve cord commissure phenotype
Ptp10D101/Ptp10D1, Ptp69Dunspecified has larval VL3/4 motor neuron phenotype
Ptp10D101/Ptp10D1, Ptp69Dunspecified has larval RP2 motor neuron phenotype
Ptp10D101/Ptp10D1, Ptp69Dunspecified has larval intersegmental nerve phenotype
Ptp10D101/Ptp10D1, Ptp69Dunspecified has larval VO2 motor neuron phenotype
Ptp10D101/Ptp10D1, Ptp69Dunspecified has larval VO1 motor neuron phenotype
The longitudinal axon bundles are irregular and often fuse to each other in Ptp10D101; Ptp69D1/Ptp69D8ex25 double mutant embryos. The outer Fas2-positive bundle is usually missing or reduced to short, discontinuous Fas2-positive regions, and breaks in the inner two bundles are also seen. Three or more Fas2-positive axon bundles that cross the midline are seen within the commissural tracts of each segment (this is not seen in wild-type embryos). Ptp69Dunspecified in double mutant combination with either Ptp10D1/Ptp10D101 or Df(1)101 results in alterations in many central nervous system axonal pathways in the embryo. The contralaterally projecting interneuronal axons of the NB 2-5 lineage cross the midline and turn anteriorly in a normal manner, but then double back across the midline after about two segments and grow posteriorly in the ipsilateral longitudinal tract. The axons of the ipsilateral intersegmental neurons grow anteriorly for a short distance and stop. The ISNd motorneuron extends an axon toward the midline that stalls and never enters the ISN root. In the NB 4-2 lineage, the RP2 axons stall before reaching their target, and the CoR axons do not branch onto all of their target muscles. The projection of local interneurons across the midline in the anterior commissure appears wild type, except that the axons always defasciculate into two bundles after crossing the midline. Ectopic interneuronal projections form on the ipsilateral side, and project across segmental boundaries in the anterior direction (these axons never form in wild type). In the NB 3-1 lineage, the RP neurons (RP1, RP3, RP4 and RP5) extend axons normally across the commissure and into the ISNb nerve, although they do not form normal synapses. The interneuronal projections are radically altered; they still cross the midline, but do not form defined anterior and posterior projections in the contralateral connective. Instead, they grow anteriorly in a circular path around the neuropil, contacting the midline at the end of their trajectory. No alterations in the numbers or positions of cell bodies of the progeny of NBs 3-1, 4-2 or 2-5 are seen in these embryos. Fas2-positive axons are seen crossing the midline in 95.5% of segments in Ptp10D1/Ptp10D101; Ptp69D4/Ptp69D8ex25 double mutant embryos (this is never seen in wild-type embryos).
