FlyBase Allele Report: Dmel\Klp64D[k1]

General Information

Symbol

Dmel\Klp64D^k1

Species

D. melanogaster

Name

FlyBase ID

FBal0102965

Feature type

allele

Associated gene

Dmel\Klp64D

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

hypomorphic allele

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

hypomorphic allele

(Jana et al., 2011)

Mutagen

ethyl methanesulfonate

(Ray et al., 1999)

Progenitor genotype

Cytology

Description

Amino acid replacement: Q13term.

(Ray et al., 1999)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

3L:5,359,218..5,359,218 [-]

Nucleotide change:

C5359218T

Reported nucleotide change:

C?T

Amino acid change:

Q13term | Klp64D-PA

Reported amino acid change:

Q13term

(Ray et al., 1999)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal auditory perception (with Klp64D^k5)

(Sarpal et al., 2003)

abnormal neuroanatomy

(Jana et al., 2011)

abnormal neuroanatomy (with Klp64D^k5)

(Jana et al., 2011)

abnormal neuroanatomy (with Klp64D^l4)

(Jana et al., 2011)

abnormal neurophysiology (with Klp64D^k5)

(Jana et al., 2011, Sarpal et al., 2003)

abnormal neurophysiology (with Klp64D^l4)

(Jana et al., 2011)

decreased rate of movement | recessive

(Ray et al., 1999)

lethal | recessive

(Ray et al., 1999)

segment polarity phenotype

(Vuong et al., 2014)

uncoordinated | adult stage | recessive

(Ray et al., 1999)

visible | somatic clone

(Vuong et al., 2014)

Phenotype Manifest In

basiconic sensillum of antennal segment 3

(Jana et al., 2011)

basiconic sensillum of antennal segment 3 (with Klp64D^k5)

(Jana et al., 2011)

basiconic sensillum of antennal segment 3 (with Klp64D^l4)

(Jana et al., 2011)

cilium

(Jana et al., 2011)

cilium (with Klp64D^k5)

(Jana et al., 2011)

cilium (with Klp64D^l4)

(Jana et al., 2011)

cilium & cell

(Avidor-Reiss et al., 2004)

denticle | ectopic

(Vuong et al., 2014)

Johnston's organ & scolopidial cilium (with Klp64Dⁿ¹²³)

(Sarpal et al., 2003)

scolopidial cilium (with Klp64Dⁿ¹²³)

(Han et al., 2003)

trichoid sensillum of antennal segment 3

(Jana et al., 2011)

trichoid sensillum of antennal segment 3 (with Klp64D^l4)

(Jana et al., 2011)

wing | somatic clone

(Vuong et al., 2014)

Detailed Description

Statement

Reference

Adult wings containing homozygous Klp64D^k1 mutant clones show severe wing margin notches. Although mutant clones are found away from the wing margin, no loss of wing tissue is seen in non-margin areas.

Heterozygous Klp64D^k1 mutant flies do not exhibit any wing phenotypes.

About 17.7% of null mutant Klp64D^k1 embryos show severe segment polarity phenotypes. Ectopic denticles are formed in the space between normal denticle belts.

(Vuong et al., 2014)

The average peak electroantennogram response is reduced in Klp64D^k1/Klp64D^l4 and Klp64D^k5/Klp64D^k1 adults compared to controls (in response to ethyl acetate, n-butanol, benzaldehyde or propionic acid).

The average cilia volume is reduced in mutant adults compared to wild type in sensilla basiconica and sensilla trichodea of the antennae.

The outer segments of the basiconic cilia of the sensilla basiconica of the antenna are less branched than normal in Klp64D^k5/Klp64D^k1 adults.

The length and volume expansions of both the basiconic and trichoid cilia are reduced at the second stage of cilia growth in Klp64D^k1/Klp64D^k1 and Klp64D^k1/Klp64D^l4 antennae compared to wild type.

(Jana et al., 2011)

In mutant animals, accumulation of microtubules is seen at the cilia base of ciliated cells.

(Avidor-Reiss et al., 2004)

Klp64D^k1/Klp64Dⁿ¹²³ mutants lack cilia in the chordotonal organs.

(Han et al., 2003)

Klp64D^k1/Klp64D^k5 homozygotes have a reduced auditory response, producing a severely reduced sound evoked potentials from the Johnston's organ neurons. In Klp64D^k1/Klp64Dⁿ¹²³ mutant animals, the sensory cilia from Johnston's organ are absent, but inner-dendritic segment and desmosomal junctions appear normal.

(Sarpal et al., 2003)

Homozygotes die at or before the early third larval instar stage in a typical crowded culture. If the homozygotes are raised in low density conditions without competition from wild-type larvae, a few mutant animals survive to be pharate pupae and occasionally eclose as fully formed adults. The adults show an acutely uncoordinated walk, are unable to stand and die in a few hours. Mutant third instar larvae show slight to severe sluggishness and roll abnormally from side to side during crawling.

(Ray et al., 1999)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Klp64D^k1/Klp64Dⁿ¹²³ has lethal phenotype, suppressible by Mmus\Kif3a^UAS.cRa/Scer\GAL4^elav.PLu

(Ray et al., 1999)

NOT Suppressor of

Statement

Reference

Klp64D[+]/Klp64D^k1 is a non-suppressor of visible phenotype of N^nd-3

(Vuong et al., 2014)

Other

Statement

Reference

Klp64D^k1, arm¹/arm[+] has visible | dominant phenotype

(Vuong et al., 2014)

Klp64D^k1, dsh³/dsh[+] has visible | dominant phenotype

(Vuong et al., 2014)

Klp64D^k1/Klp64Dⁿ¹²³, Mmus\Kif3a^UAS.cRa, Scer\GAL4^elav.PLu has viable phenotype

(Ray et al., 1999)

Phenotype Manifest In

NOT Suppressor of

Statement

Reference

Klp64D[+]/Klp64D^k1 is a non-suppressor of wing phenotype of N^nd-3

(Vuong et al., 2014)

Other

Statement

Reference

Klp64D^k1, arm¹/arm[+] has wing margin phenotype

(Vuong et al., 2014)

Klp64D^k1, arm¹/arm[+] has wing margin bristle phenotype

(Vuong et al., 2014)

Klp64D^k1, dsh³/dsh[+] has wing margin phenotype

(Vuong et al., 2014)

Klp64D^k1, dsh³/dsh[+] has wing margin bristle phenotype

(Vuong et al., 2014)

Additional Comments

Genetic Interactions

Statement

Reference

One copy of Klp64D^k1 is unable to enhance the wing notching seen in N^nd-3 heterozygotes.

One copy of Klp64D^k1 is unable to enhance the wing notching seen in N^264-40 heterozygotes.

In contrast to either mutation alone, more than 50% of flies heterozygous for Klp64D^k1 and arm¹ show significant loss of wing bristles and wing margin notching.

In contrast to either mutation alone, flies heterozygous for Klp64D^k1 and dsh³ show loss of wing bristles and wing margin notching.

(Vuong et al., 2014)

Xenogenetic Interactions

Statement

Reference

The lethality of Klp64D^k1/Klp64Dⁿ¹²³ flies is completely rescued by Mmus\Kif3a^Scer\UAS.cRa expressed under the control of Scer\GAL4^elav.PLu.

(Ray et al., 1999)

Complementation and Rescue Data

Fails to complement

Klp64Dⁿ¹²³

(Ray et al., 1999)

Rescued by

Klp64D^k1 is rescued by Klp64D^UAS.GFP/Scer\GAL4^SG18.1

(Jana et al., 2011)

Klp64D^k1 is rescued by Klp64D^UAS.GFP/Scer\GAL4^ChAT.7.4

(Jana et al., 2011)

Klp64D^k5/Klp64D^k1 is rescued by Scer\GAL4^da.G32/Klp64D^UAS.cRa

(Jana et al., 2011)

Klp64D^k1/Klp64Dⁿ¹²³ is rescued by Klp64D^UAS.cRa

(Ray et al., 1999)

Klp64D^k1 is rescued by Klp64D^UAS.cRa

(Ray et al., 1999)

Comments

Expression of Klp64D^Scer\UAS.cRa under the control of Scer\GAL4^da.G32 rescues the reduced electroantennogram response of Klp64D^k5/Klp64D^k1 adults in response to ethyl acetate, n-butanol, benzaldehyde or propionic acid.

Expression of Klp64D^{Scer\UAS.T:Avic\GFP} under the control of either Scer\GAL4^SG18.1 or Scer\GAL4^Cha.7.4 rescues the reduced electroantennogram response of homozygous Klp64D^k1 adults in response to ethyl acetate, n-butanol, benzaldehyde or propionic acid.

(Jana et al., 2011)

Klp64D^Scer\UAS.cRa rescues the lethality and uncoordinated behaviour of Klp64D^k1, either in homozygous condition or in transheterozygous combination with Klp64Dⁿ¹²³, in the absence of a Scer\GAL4 driver.

(Ray et al., 1999)

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

5578

y¹ w¹; Klp64D^k1/TM3, y⁺ Ser¹

Kyoto

108311

y¹ w¹; Klp64D^k1/TM3, y⁺ Ser¹

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Klp64D^k1

(Vuong et al., 2020, Dey and Ray, 2018, Kulkarni et al., 2017, Park et al., 2016, Vuong et al., 2014, Sadananda et al., 2012, Jana et al., 2011, Mattie et al., 2010, Christensen et al., 2008.7.11, Lee et al., 2008, Christensen et al., 2007.10.29, Christensen et al., 2007.10.29, Baqri et al., 2006)

klp64D^k1

(White et al., 2020, Avidor-Reiss et al., 2004)

Name Synonyms

Secondary FlyBase IDs

References (18)

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