FlyBase Allele Report: Dmel\Klp64D[l4]

General Information

Symbol

Dmel\Klp64D^l4

Species

D. melanogaster

Name

FlyBase ID

FBal0156302

Feature type

allele

Associated gene

Dmel\Klp64D

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

Nature of the Allele

Allele class

Mutagen

Progenitor genotype

Cytology

Description

Amino acid replacement: G101D.

(Sarpal et al., 2003)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

3L:5,358,953..5,358,953 [-]

Nucleotide change:

G5358953A

Amino acid change:

G101D | Klp64D-PA

Reported amino acid change:

G101D

Comment:

Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

(Sarpal et al., 2003)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal auditory perception (with Klp64D^k5)

(Sarpal et al., 2003)

abnormal neuroanatomy (with Klp64D^k1)

(Jana et al., 2011)

abnormal neuroanatomy (with Klp64D^k5)

(Jana et al., 2011)

abnormal neurophysiology (with Klp64D^k1)

(Jana et al., 2011)

abnormal neurophysiology (with Klp64D^k5)

(Jana et al., 2011, Sarpal et al., 2003)

lethal | recessive

(Sarpal et al., 2003)

Phenotype Manifest In

basiconic sensillum of antennal segment 3 (with Klp64D^k1)

(Jana et al., 2011)

basiconic sensillum of antennal segment 3 (with Klp64D^k5)

(Jana et al., 2011)

cilium (with Klp64D^k1)

(Jana et al., 2011)

cilium (with Klp64D^k5)

(Jana et al., 2011)

Johnston organ (with Klp64D^k5)

(Sarpal et al., 2003)

scolopidial cilium & Johnston's organ (with Klp64D^k5)

(Sarpal et al., 2003)

trichoid sensillum of antennal segment 3 (with Klp64D^k1)

(Jana et al., 2011)

Detailed Description

Statement

Reference

The average peak electroantennogram response is reduced in Klp64D^k1/Klp64D^l4 and Klp64D^k5/Klp64D^l4 adults compared to controls (in response to ethyl acetate, n-butanol, benzaldehyde or propionic acid).

The electron-dense basal bodies and the connecting cilium are often present in the sensilla basiconica of the antenna in Klp64D^k5/Klp64D^l4 adults, but the outer segments of the basiconic cilia usually contain a slender extension with several singlet microtubules.

The length and volume expansions of both the basiconic and trichoid cilia are reduced at the second stage of cilia growth in Klp64D^k1/Klp64D^l4 antennae compared to wild type.

(Jana et al., 2011)

Klp64D^l4/Klp64D^k5 homozygotes have a reduced auditory response, producing a severely reduced sound evoked potentials from the Johnston's organ neurons.

(Sarpal et al., 2003)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Klp64D^k5/Klp64D^l4 has abnormal auditory perception phenotype, enhanceable by Kap3^V6

(Sarpal et al., 2003)

Klp64D^k5/Klp64D^l4 has abnormal neurophysiology phenotype, enhanceable by Kap3^V6

(Sarpal et al., 2003)

Other

Statement

Reference

Kap3^V6, Klp64D^k5/Klp64D^l4 has lethal phenotype

(Sarpal et al., 2003)

Kap3^V6, Klp64D^k5/Klp64D^l4 has partially lethal phenotype

(Sarpal et al., 2003)

Phenotype Manifest In

Enhanced by

Statement

Reference

Klp64D^k5/Klp64D^l4 has Johnston organ phenotype, enhanceable by Kap3^V6

(Sarpal et al., 2003)

Klp64D^k5/Klp64D^l4 has scolopidial cilium & Johnston's organ phenotype, enhanceable by Kap3^V6

(Sarpal et al., 2003)

Additional Comments

Genetic Interactions

Statement

Reference

The addition of Kap3^V6/+ significantly enhances the recessive lethality of Klp64D^l4/Klp64D^k5 animals. The addition of Kap3^V6/Y causes complete lethality. The addition of Kap3^V6/+ to Klp64D^l4/Klp64D^k5 also completely abolishes the sound evoked potentials usually obtained from the Johnston's organ neurons, and enhances the ciliary defects seen in these animals.

(Sarpal et al., 2003)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

Klp64D^l4

(Jana et al., 2011, Lee et al., 2008)

Name Synonyms

Secondary FlyBase IDs

References (3)

Report Sections

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