FlyBase Allele Report: Scer\GAL4[Act5C.PHb]

General Information

Symbol

Scer\GAL4^Act5C.PHb

Species

S. cerevisiae

Name

Actin 5C promoter construct of Hiromi

FlyBase ID

FBal0097155

Feature type

allele

Associated gene

Scer\GAL4

Associated Insertion(s)

Carried in Construct

P{Act5C-GAL4.Hb}

Also Known As

Act5C-GAL4, Act25-GAL4, pWA-GAL4, pWAGAL4, actin 5C-Gal4, actin5C-Gal4, P{w^+mC=A5c-GAL4}

Transgenic product class

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

Mutagen

in vitro construct

(Ishikawa et al., 1999)

Progenitor genotype

Carried in construct

P{Act5C-GAL4.Hb}

Cytology

Description

Act5C promoter sequences regulate expression of a GAL4 driver.

(Ishikawa et al., 1999)

Allele components

Component

Use(s)

Regulatory region(s)

Act5C

Encoded product / tool

GAL4

binary expression system - driver

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

distribution deduced from reporter (Gal4 UAS)

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

organism | ubiquitous

(Ishikawa et al., 1999)

Additional Information

Statement

Reference

Drives ubiquitous adult expression.

(FlyBase Curators, 2017)

Used as component of binary system in cell culture, to drive UAS construct of gene of interest.

(Usui et al., 1999)

Expression is observed in all adult cells.

(Ishikawa et al., 1999)

Marker for

Reflects expression of

Act5C

(Ishikawa et al., 1999, Usui et al., 1999)

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal locomotor behavior, with Pcmt^UAS.cCa

(Chavous et al., 2001)

abnormal locomotor rhythm, with cry^UAS.cIa

(Ishikawa et al., 1999)

chemical resistant, with TrxT^C35A.UAS

(Umeda-Kameyama et al., 2007)

chemical resistant, with TrxT^{D26A.K57I.UAS}

(Umeda-Kameyama et al., 2007)

chemical resistant, with TrxT^UAS.cUa

(Umeda-Kameyama et al., 2007)

increased cell death, with rpr^UAS.cIa

(Igaki et al., 2002, Kuranaga et al., 2002)

increased cell death, with Traf4^UAS.Tag:HA

(Kuranaga et al., 2002)

lethal, with β4GalT7^RNAi.C.UAS

(Takemae et al., 2003)

lethal, with β4GalT7^RNAi.N.UAS

(Takemae et al., 2003)

lethal | heat sensitive, with rt^RNAi.UAS

(Ichimiya et al., 2004)

lethal - all die before end of larval stage, with Dref^RNAi.h-h.UAS

(Yoshida et al., 2004)

lethal - all die before end of larval stage, with pic^RNAi.650.UAS

(Takata et al., 2004)

lethal - all die before end of pupal stage, with lark^UAS.Tag:HA

(Schroeder and Jackson, 2002)

lethal - all die before end of pupal stage, with sll^RNAi.UAS

(Kamiyama et al., 2003)

long lived, with Pcmt^UAS.cCa

(Chavous et al., 2001)

partially lethal | heat sensitive, with tw^RNAi.UAS.cIa

(Ichimiya et al., 2004)

some die during larval stage, with Dref^RNAi.h-h.UAS

(Yoshida et al., 2004)

some die during larval stage, with pic^RNAi.650.UAS

(Takata et al., 2004)

some die during pupal stage, with lark^UAS.Tag:HA

(Schroeder and Jackson, 2002)

some die during pupal stage, with sll^RNAi.UAS

(Kamiyama et al., 2003)

Phenotype Manifest In

actin filament, with Hem^{UAS.Tag:Myr(Src64B)}

(Bogdan and Klambt, 2003)

adult abdomen, with rt^RNAi.UAS

(Ichimiya et al., 2004)

adult abdomen, with tw^RNAi.UAS.cIa

(Ichimiya et al., 2004)

spindle, with Gβ13F::mira^UAS.cFa

(Fuse et al., 2003)

Detailed Description

Statement

Reference

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Scer\GAL4^Act5C.PHb, rpr^UAS.cIa has increased cell death phenotype, suppressible by BacA\p35^UAS.cHa, Scer\GAL4^Act5C.PHb

(Igaki et al., 2002)

Scer\GAL4^Act5C.PHb, rpr^UAS.cIa has increased cell death phenotype, suppressible | partially by Diap1^UAS.Tag:FLAG, Scer\GAL4^Act5C.PHb

(Kuranaga et al., 2002)

Scer\GAL4^Act5C.PHb, Traf4^UAS.Tag:HA has increased cell death phenotype, suppressible by Diap1^UAS.Tag:FLAG, Scer\GAL4^Act5C.PHb

(Kuranaga et al., 2002)

Suppressor of

Statement

Reference

BacA\p35^UAS.cHa, Scer\GAL4^Act5C.PHb is a suppressor of increased cell death phenotype of Scer\GAL4^Act5C.PHb, rpr^UAS.cIa

(Igaki et al., 2002)

Diap1^UAS.Tag:FLAG, Scer\GAL4^Act5C.PHb is a suppressor | partially of increased cell death phenotype of Scer\GAL4^Act5C.PHb, rpr^UAS.cIa

(Kuranaga et al., 2002)

Diap1^UAS.Tag:FLAG, Scer\GAL4^Act5C.PHb is a suppressor of increased cell death phenotype of Scer\GAL4^Act5C.PHb, Traf4^UAS.Tag:HA

(Kuranaga et al., 2002)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Y. Hiromi

(Hayashi and Yamaguchi, 1999, Ishikawa et al., 1999)

Comments

Carried in plasmid "pAct5C-Gal4", transfected into Kc cells and used in transcription activation assays.

(Hayashi and Yamaguchi, 1999)

Carried in plasmid "pWAGAL4", transfected into S2 cells and used to drive expression of Rala in these cells.

(Sawamoto et al., 1999)

Carried in a plasmid and transfected into S2 cells to drive stan expression.

(Usui et al., 1999)

Carried in a plasmid "pWAGAL4" transfected into S2 cells and used to drive expression of transfected Scer\UAS constructs.

(Igaki et al., 2000)

Carried in a plasmid and transfected into S2 cells to drive D expression contructs.

(Oda and Tsukita, 1999)

Carried in plasmid "pWAGAL4" and transfected into S2 cells as a driver construct.

(Igaki et al., 2002, Verkhusha et al., 2001)

Carried in plasmid "act5c-GAL4" transfected into S2 cells.

(Bogdan and Klambt, 2003)

Carried in plasmid "actin-promoter-GAL4", transfected into S2 cells.

(Iourgenko et al., 2003)

Carried in plasmid "pAct-Gal4" and transfected into S2 cells.

(Ogden et al., 2003)

Carried in plasmid "pWA-GAL4" and transfected into S2 cells as a driver construct.

(Hori et al., 2004)

Carried in plasmid "pWAGAL4", transfected into S2 cells and used as a driver construct.

(Umeda-Kameyama et al., 2007, Hirota et al., 2005, Hirota et al., 2002, Hirota et al., 2002, Kanda et al., 2002)

Carried in plasmid "pA5c-GAL4", transfected into S2 cells and used as a driver construct.

(Casso et al., 2005)

Expressed in Drosophila Schneider cells and used to drive expression of Hsap\PQBP1^Scer\UAS.cYa.

(Yoshimura et al., 2006)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Scer\GAL4^Act5C.PHb

pWAGAL4

(Kanuka et al., 2005)

Name Synonyms

Actin 5C promoter construct of Hiromi

(Ishikawa et al., 1999)

Secondary FlyBase IDs

References (44)

Report Sections

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