FlyBase Allele Report: Dmel\CycB[2]

General Information

Symbol

Dmel\CycB²

Species

D. melanogaster

Name

FlyBase ID

FBal0094855

Feature type

allele

Associated gene

Dmel\CycB

Associated Insertion(s)

Carried in Construct

Key Links

Allele class

loss of function allele

Mutagen

Delta2-3 transposase

Nature of the Allele

Allele class

loss of function allele

(Jacobs et al., 1998)

Mutagen

Delta2-3 transposase

(Jacobs et al., 1998)

Progenitor genotype

CycB¹

(Jacobs et al., 1998)

Cytology

Description

Imprecise excision of P-element resulting in small deletion removing the region encoding α-helix 1, a conserved element of the cyclin box.

(Jacobs et al., 1998)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates cancer

modeled by Delta^UAS.cDa, Not3^KK102144

(Vicente et al., 2018)

modeled by Delta^UAS.cDa, twin^KK108897

(Vicente et al., 2018)

ameliorates germ cell cancer

modeled by snf¹⁴⁸

(Chau et al., 2009)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal cell number | oogenesis

(Flora et al., 2018)

abnormal cytokinesis

(Echard and O'Farrell, 2003)

abnormal mitotic cell cycle

(Echard and O'Farrell, 2003)

abnormal mitotic cell cycle | embryonic stage

(Aydogan et al., 2018)

female sterile

(Jacobs et al., 1998)

male sterile

(Jacobs et al., 1998)

viable

(Jacobs et al., 1998)

Phenotype Manifest In

embryonic abdominal segment (with CycB³), with CycB^2xNRE.hb

(Kadyrova et al., 2007)

female germline cell

(Chau et al., 2009)

female germline cell | oogenesis

(Flora et al., 2018)

female germline stem cell | oogenesis

(Flora et al., 2018)

filzkorper (with CycB³), with CycB^2xNRE.hb

(Kadyrova et al., 2007)

germarium

(Flora et al., 2018)

ovary

(Jacobs et al., 1998)

Detailed Description

Statement

Reference

CycB²/+ embryos exhibit a significant increase in S-phase length while they do not exhibit any changes in centriole growth rate, growth period or size when compared to controls.

(Aydogan et al., 2018)

CycB² homozygous germaria show an abnormal accumulation of undifferentiated, pre-cystoblast cells, as compared to controls, although about of third of them completely lack germline stem cells. These mutant germaria show an increased number of spectrosome-containing single cells, as compared to controls.

(Flora et al., 2018)

Mutant ovaries contain few or no germ cells.

(Chau et al., 2009)

Over 95% of embryos derived from CycB²/CycB³ ; CycB^2xNRE.hb females have defects in the abdominal segments, the posterior terminalia, or both. In extreme cases, the embryos die with an open posterior hole and not segments following the third thoracic one. The defects appear to arise during nuclear cleavage cycles 9-13; the somatic nuclei in the posterior of the embryos delay progression through cycles 9-13 and ultimately lose contact with the embryonic cortex, falling into the interior.

(Kadyrova et al., 2007)

Mutant embryos appear to start cytokinesis earlier than wild-type embryos. These embryos also appear to stay in cytokinesis longer than normal.

(Echard and O'Farrell, 2003)

Mutant females have rudimentary ovaries and lay few eggs. Homozygotes and hemizygotes reach adulthood.

(Jacobs et al., 1998)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

CycB[+]/CycB² is an enhancer of abnormal cell cycle phenotype of Det^{S125E.UASp.GFP}, Scer\GAL4^{VP16.nanos.UTR}

(Mathieu et al., 2013)

CycB^KG08886/CycB² is an enhancer of abnormal cell cycle phenotype of Det^{S125E.UASp.GFP}, Scer\GAL4^{VP16.nanos.UTR}

(Mathieu et al., 2013)

CycB² is an enhancer of visible phenotype of H^UAS.cMa, Scer\GAL4^GMR.PF

(Müller et al., 2005)

CycB[+]/CycB² is an enhancer of abnormal mitotic cell cycle | maternal effect phenotype of png¹⁰⁵⁸/png³³¹⁸

(Lee et al., 2001)

NOT Enhancer of

Statement

Reference

CycB² is a non-enhancer of visible phenotype of upd1^GMR.PB

(Mukherjee et al., 2006)

Suppressor of

Statement

Reference

CycB²/CycB² is a suppressor of increased cell number | recessive | dorsal closure stage phenotype of fzr^ie28

(Drechsler et al., 2018)

CycB² is a suppressor | partially of visible | adult stage phenotype of Delta^UAS.cDa, Scer\GAL4^ey.PH, twin^KK108897

(Vicente et al., 2018)

CycB² is a suppressor | partially of neoplasia | adult stage phenotype of Delta^UAS.cDa, Scer\GAL4^ey.PH, twin^KK108897

(Vicente et al., 2018)

CycB² is a suppressor of visible | adult stage phenotype of Delta^UAS.cDa, Not3^KK102144, Scer\GAL4^ey.PH

(Vicente et al., 2018)

CycB² is a suppressor of neoplasia | adult stage phenotype of Delta^UAS.cDa, Not3^KK102144, Scer\GAL4^ey.PH

(Vicente et al., 2018)

CycB[+]/CycB² is a suppressor of increased cell death phenotype of Scer\GAL4^hh.PU, bsk^KK108156, mad2^GD16427

(Wong et al., 2014)

CycB[+]/CycB² is a suppressor of increased cell death phenotype of Scer\GAL4^en.PU, bsk^KK108156, mad2^GD16427

(Wong et al., 2014)

CycB[+]/CycB² is a suppressor | partially of abnormal mitotic cell cycle | maternal effect | embryonic stage phenotype of Scer\GAL4^αTub84B.PL, Xpd^SH2137, Xpd^UAS.Tag:V5

(Li et al., 2010)

CycB[+]/CycB² is a suppressor | maternal effect | partially of abnormal mitotic cell cycle | maternal effect | embryonic cycle 14 phenotype of Df(3L)Cat/Capr², Fmr1³/Fmr1[+]

(Papoulas et al., 2010)

CycB[+]/CycB² is a suppressor of visible phenotype of Scer\GAL4^en-e16E, crol^UAS.cMa

(Mitchell et al., 2008)

CycB[+], CycB3^L6540, CycB², CycB3[+] is a suppressor | partially of visible phenotype of Scer\GAL4^GMR.PF, pbl^{ΔDH497-549.UAS}

(Gregory et al., 2007)

CycB[+]/CycB² is a suppressor of visible phenotype of E(mus304)[+]/PolA1^Emus304, mei-41^29D

(LaRocque et al., 2007)

NOT Suppressor of

Statement

Reference

CycB[+]/CycB² is a non-suppressor of increased cell number | recessive | dorsal closure stage phenotype of fzr^ie28

(Drechsler et al., 2018)

CycB² is a non-suppressor of visible phenotype of upd1^GMR.PB

(Mukherjee et al., 2006)

Other

Statement

Reference

CycB[+]/CycB², Scer\GAL4^unspecified, bw^D, crol^unspecified has abnormal eye color | dominant phenotype

(Schneiderman et al., 2010)

CycB[+]/CycB², CycD¹ has viable phenotype

(Emmerich et al., 2004)

CycB[+]/CycB², CycD² has partially lethal phenotype

(Emmerich et al., 2004)

CycB², CycD¹ has viable phenotype

(Emmerich et al., 2004)

CycA^C8LR1, CycB² has decreased occurrence of cell division | embryonic cycle 15 phenotype

(Jacobs et al., 1998)

CycB3^L6540, CycB² has lethal phenotype

(Jacobs et al., 1998)

CycB3³, CycB² has lethal phenotype

(Jacobs et al., 1998)

CycB3², CycB² has lethal phenotype

(Jacobs et al., 1998)

Phenotype Manifest In

Enhancer of

Statement

Reference

CycB[+]/CycB² is an enhancer of nurse cell phenotype of Det^{S125E.UASp.GFP}, Scer\GAL4^{VP16.nanos.UTR}

(Mathieu et al., 2013)

CycB[+]/CycB² is an enhancer of oocyte phenotype of Det^{S125E.UASp.GFP}, Scer\GAL4^{VP16.nanos.UTR}

(Mathieu et al., 2013)

CycB[+]/CycB² is an enhancer of female germline cyst phenotype of Det^{S125E.UASp.GFP}, Scer\GAL4^{VP16.nanos.UTR}

(Mathieu et al., 2013)

CycB[+]/CycB² is an enhancer of female fusome phenotype of Det^{S125E.UASp.GFP}, Scer\GAL4^{VP16.nanos.UTR}

(Mathieu et al., 2013)

CycB^KG08886/CycB² is an enhancer of egg chamber phenotype of Det^{S125E.UASp.GFP}, Scer\GAL4^{VP16.nanos.UTR}

(Mathieu et al., 2013)

CycB^KG08886/CycB² is an enhancer of ring canal phenotype of Det^{S125E.UASp.GFP}, Scer\GAL4^{VP16.nanos.UTR}

(Mathieu et al., 2013)

CycB^KG08886/CycB² is an enhancer of nurse cell phenotype of Det^{S125E.UASp.GFP}, Scer\GAL4^{VP16.nanos.UTR}

(Mathieu et al., 2013)

CycB^KG08886/CycB² is an enhancer of oocyte phenotype of Det^{S125E.UASp.GFP}, Scer\GAL4^{VP16.nanos.UTR}

(Mathieu et al., 2013)

CycB^KG08886/CycB² is an enhancer of female germline cyst phenotype of Det^{S125E.UASp.GFP}, Scer\GAL4^{VP16.nanos.UTR}

(Mathieu et al., 2013)

CycB^KG08886/CycB² is an enhancer of female fusome phenotype of Det^{S125E.UASp.GFP}, Scer\GAL4^{VP16.nanos.UTR}

(Mathieu et al., 2013)

CycB[+]/CycB² is an enhancer of egg chamber phenotype of Det^{S125E.UASp.GFP}, Scer\GAL4^{VP16.nanos.UTR}

(Mathieu et al., 2013)

CycB[+]/CycB² is an enhancer of ring canal phenotype of Det^{S125E.UASp.GFP}, Scer\GAL4^{VP16.nanos.UTR}

(Mathieu et al., 2013)

CycB² is an enhancer of eye phenotype of H^UAS.cMa, Scer\GAL4^GMR.PF

(Müller et al., 2005)

CycB² is an enhancer of cardioblast phenotype of CycA^C8LR1

(Han and Bodmer, 2003)

CycB² is an enhancer of mitotic cell cycle phenotype of CycA^C8LR1

(Han and Bodmer, 2003)

NOT Enhancer of

Statement

Reference

CycB² is a non-enhancer of eye phenotype of upd1^GMR.PB

(Mukherjee et al., 2006)

CycB² is a non-enhancer of phenotype of Myb^unspecified

(Fung et al., 2003)

Suppressor of

Statement

Reference

CycB²/CycB² is a suppressor of embryonic somatic muscle cell phenotype of fzr^ie28

(Drechsler et al., 2018)

CycB²/CycB² is a suppressor of embryonic myoblast | dorsal closure stage phenotype of fzr^ie28

(Drechsler et al., 2018)

CycB² is a suppressor | partially of eye phenotype of Delta^UAS.cDa, Scer\GAL4^ey.PH, twin^KK108897

(Vicente et al., 2018)

CycB² is a suppressor of eye phenotype of Delta^UAS.cDa, Not3^KK102144, Scer\GAL4^ey.PH

(Vicente et al., 2018)

CycB[+]/CycB² is a suppressor of wing blade posterior compartment phenotype of Scer\GAL4^en-e16E, crol^UAS.cMa

(Mitchell et al., 2008)

CycB[+], CycB3^L6540, CycB², CycB3[+] is a suppressor | partially of eye phenotype of Scer\GAL4^GMR.PF, pbl^{ΔDH497-549.UAS}

(Gregory et al., 2007)

CycB[+]/CycB² is a suppressor of eye phenotype of E(mus304)[+]/PolA1^Emus304, mei-41^29D

(LaRocque et al., 2007)

NOT Suppressor of

Statement

Reference

CycB[+]/CycB² is a non-suppressor of embryonic somatic muscle cell phenotype of fzr^ie28

(Drechsler et al., 2018)

CycB[+]/CycB² is a non-suppressor of embryonic myoblast | dorsal closure stage phenotype of fzr^ie28

(Drechsler et al., 2018)

CycB² is a non-suppressor of eye phenotype of upd1^GMR.PB

(Mukherjee et al., 2006)

CycB² is a non-suppressor of phenotype of Myb^unspecified

(Fung et al., 2003)

Other

Statement

Reference

CycB[+]/CycB², Scer\GAL4^unspecified, bw^D, crol^unspecified has eye phenotype

(Schneiderman et al., 2010)

CycA^C8LR1, CycB² has mitotic spindle | embryonic cycle 13 phenotype

(Jacobs et al., 1998)

CycB3³, CycB² has embryo | embryonic cycle 15 phenotype

(Jacobs et al., 1998)

CycB3^L6540, CycB² has spindle phenotype

(Jacobs et al., 1998)

CycB3^L6540, CycB² has embryo | embryonic cycle 15 phenotype

(Jacobs et al., 1998)

Additional Comments

Genetic Interactions

Statement

Reference

Cell death induced by bsk knockdown in cells exhibiting chromosomal instability (through expression of mad2^GD16427) is reduced by lengthening G[[2]] through a CycB² heterozygous background.

(Wong et al., 2014)

Reducing the level of CycB, through a CycB²/+ background, greatly enhances the Scer\GAL4^{nos.UTR.T:Hsim\VP16}>Det^{S125E.Scer\UAS.P\T.T:Avic\GFP} gain-of-function phenotype. Further reduction in the level of CycB, through a CycB²/CycB^KG08886 background, greatly enhances the Scer\GAL4^{nos.UTR.T:Hsim\VP16}>Det^{S125E.Scer\UAS.P\T.T:Avic\GFP} gain-of-function phenotype.

(Mathieu et al., 2013)

The nuclear division defects seen in embryos derived from homozygous Xpd^SH2137 females expressing Xpd^{Scer\UAS.T:SV5\V5} under the control of Scer\GAL4^αTub84B.PL are partially rescued if the females are carrying CycB²/+.

(Li et al., 2010)

Reduction of maternal CycB by one half (using the CycB² allele) in the Capr²/Df(3L)Cat, Fmr1³/+ background partially rescues the premature mitosis 14 phenotype seen in embryos of this genotype. (Reduction of the zygotic contribution of CycB has no effect.)

(Papoulas et al., 2010)

snf¹⁴⁸ ; CycB² double mutant ovaries are not tumourous and contain few or no germ cells, similar to the CycB² single mutant phenotype.

(Chau et al., 2009)

The reduced size of the posterior compartment of the wing that is seen in animals expressing crol^Scer\UAS.cMa under the control of Scer\GAL4^en-e16E is suppressed if they are also heterozygous for CycB².

(Mitchell et al., 2008)

The lethality seen in mei-41^29D males in which excision of an insertion of P{hsw^a} is induced during development is partly suppressed by CycB²/+.

The lethality seen in mei-41^29D males in which excision of an insertion of P{hsw^a} is induced during development is completely suppressed if the female parent carries CycA^C8LR1 and if the male progeny carries both CycA^C8LR1 and CycB².

(LaRocque et al., 2007)

The rough eye phenotype seen in mei-41^29D ; DNApol-α180^Emus304/+ adults is partially rescued by CycB²/+.

(LaRocque et al., 2007)

Adult viability of CycD²/Y ; CycB²/+ males is 90% of expected value.

(Emmerich et al., 2004)

The addition of CycB² has no effect on Myb^unspecified viability.

(Fung et al., 2003)

CycA^C8LR1, CycB² double mutants arrest mesodermal division at mitosis 15 a more severe phenotype than CycA^C8LR1 alone. Also a further reduction in the number of myocardial cells is seen.

(Han and Bodmer, 2003)

CycB3^L6540 CycB^- (CycB² or Df(2R)599-5) double mutants have defective late mitotic figures during mitosis 15 and multipolar spindles at stage 13.

(Jacobs et al., 1998)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

CycB¹/CycB² is rescued by CycB^UASp.GFP

(Mathieu et al., 2013)

CycB¹/CycB² is rescued by CycB^5E.UASp.GFP

(Mathieu et al., 2013)