FlyBase Allele Report: Dmel\sktl[Δ15]

General Information

Symbol

Dmel\sktl^Δ15

Species

D. melanogaster

Name

FlyBase ID

FBal0094345

Feature type

allele

Associated gene

Dmel\sktl

Associated Insertion(s)

Carried in Construct

Key Links

Allele class

loss of function allele

Mutagen

Delta2-3 transposase

Nature of the Allele

Allele class

loss of function allele

(Hassan et al., 1998)

Mutagen

Delta2-3 transposase

(Hassan et al., 1998)

Progenitor genotype

sktl^k07505

(Hassan et al., 1998)

Cytology

Description

Deletion removing the P{lacW} and 0.7kb of flanking genomic sequence.

(Hassan et al., 1998)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

dorsal appendage

(Hassan et al., 1998)

egg

(Hassan et al., 1998)

egg | maternal effect (with sktl^2.3)

(Gervais et al., 2008)

egg chamber | germline clone

(Hassan et al., 1998)

nurse cell & nucleus

(Hassan et al., 1998)

oocyte nucleus | oogenesis stage S9 (with sktl^2.3)

(Gervais et al., 2008)

oocyte nucleus | oogenesis stage S10 (with sktl^2.3)

(Gervais et al., 2008)

ovary

(Hassan et al., 1998)

wing

(Hassan et al., 1998)

wing & macrochaeta

(Hassan et al., 1998)

Detailed Description

Statement

Reference

sktl^2.3/sktl^Δ15 females show defects in the localisation of the oocyte nucleus (31% of stage 9-10 oocytes show this phenotype) and they lay eggs that are ventralised.

(Gervais et al., 2008)

Lethality acts in the late first larval instar. Mutants show no morphological abnormalities. When the sktl^Δ15 chromosome is heterozygous with the sktl^k07505, insc^k07505 chromosome, late second instar lethality results. No abnormalities were detectable in the larval neuromuscular junction electrophysiological preparation indicating no defect in vesicular trafficking nor dense core vesicle secretion. Germ line clone analysis revealed arrest at stage 10 of oogenesis. Arrested egg chambers show defects in nurse cell nuclei. The few eggs that develop are small and show defects in the dorsal appendages. Clonal analysis in the wing revealed a requirement for sktl for cell viability and/or growth. The few bristles that are recovered in clones are wavy or bent, indicating cytoskeletal defects. Clonal analysis in the eye produced no mutant clones, suggesting a requirement for sktl for cell viability or cell division in imaginal discs.

(Hassan et al., 1998)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

sktl[+]/sktl^Δ15 is an enhancer of lethal phenotype of ash2¹⁸/ash2¹

(Cheng and Shearn, 2004)

Other

Statement

Reference

ash1²², sktl[+]/sktl^Δ15 has visible | dominant | homeotic phenotype

(Cheng and Shearn, 2004)

ash1²², sktl^Δ15 has visible | dominant | homeotic phenotype

(Cheng and Shearn, 2004)

ash2¹⁸, sktl^Δ15 has visible | dominant | homeotic phenotype

(Cheng and Shearn, 2004)

ash2², sktl^Δ15 has visible | dominant | homeotic phenotype

(Cheng and Shearn, 2004)

ash2¹⁸/ash2¹, sktl[+]/sktl^Δ15 has abnormal developmental rate phenotype

(Cheng and Shearn, 2004)

ash2¹⁸, sktl[+]/sktl^Δ15 has visible | dominant | homeotic phenotype

(Cheng and Shearn, 2004)

ash2², sktl[+]/sktl^Δ15 has visible | dominant | homeotic phenotype

(Cheng and Shearn, 2004)

ash2¹⁸/ash2¹, sktl^Δ15 has abnormal developmental rate phenotype

(Cheng and Shearn, 2004)

Phenotype Manifest In

Enhanced by

Statement

Reference

sktl^Δ15/sktl^2.3 has oocyte nucleus | oogenesis stage S9 phenotype, enhanceable by baz⁴/baz[+]

(Gervais et al., 2008)

sktl^Δ15/sktl^2.3 has oocyte nucleus | oogenesis stage S10 phenotype, enhanceable by baz⁴/baz[+]

(Gervais et al., 2008)

Other

Statement

Reference

ash1²², sktl[+]/sktl^Δ15 has mesothoracic leg phenotype

(Cheng and Shearn, 2004)

ash1²², sktl^Δ15 has mesothoracic leg phenotype

(Cheng and Shearn, 2004)

ash1²², sktl^Δ15 has metathoracic leg | ectopic phenotype

(Cheng and Shearn, 2004)

ash2¹⁸, sktl^Δ15 has mesothoracic leg phenotype

(Cheng and Shearn, 2004)

ash2¹⁸, sktl^Δ15 has metathoracic leg | ectopic phenotype

(Cheng and Shearn, 2004)

ash2², sktl^Δ15 has mesothoracic leg phenotype

(Cheng and Shearn, 2004)

ash2², sktl^Δ15 has metathoracic leg | ectopic phenotype

(Cheng and Shearn, 2004)

ash1²², sktl[+]/sktl^Δ15 has metathoracic leg | ectopic phenotype

(Cheng and Shearn, 2004)

ash2¹⁸, sktl[+]/sktl^Δ15 has mesothoracic leg phenotype

(Cheng and Shearn, 2004)

ash2¹⁸, sktl[+]/sktl^Δ15 has metathoracic leg | ectopic phenotype

(Cheng and Shearn, 2004)

ash2², sktl[+]/sktl^Δ15 has mesothoracic leg phenotype

(Cheng and Shearn, 2004)

ash2², sktl[+]/sktl^Δ15 has metathoracic leg | ectopic phenotype

(Cheng and Shearn, 2004)

Additional Comments

Genetic Interactions

Statement

Reference

baz⁴/+ enhances the nucleus mislocalisation defect seen in stage 9-10 sktl^2.3/sktl^Δ15 oocytes, such that 63% have a mislocalised nucleus.

(Gervais et al., 2008)

ash1²² sktl^Δ15 double heterozygotes show a homeotic transformation of third leg to second leg (3.7% penetrance). ash2² sktl^Δ15 double heterozygotes show a homeotic transformation of third leg to second leg (28.6% penetrance). ash2¹⁸ sktl^Δ15 double heterozygotes show a homeotic transformation of third leg to second leg (9.4% penetrance). sktl^Δ15/+ ; ash2¹/ash2¹⁸ larvae have delayed development and die at an earlier stage than ash2¹/ash2¹⁸ larvae.

(Cheng and Shearn, 2004)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to complement

sktl⁰⁵⁴⁷⁵

(Prokopenko et al., 2000)

sktl^k07505

(Hassan et al., 1998)

Comments

Complements insc⁸ and insc²².

(Hassan et al., 1998)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

sktl^Δ15

(Kim et al., 2021, Bothe et al., 2014, Gervais et al., 2008)

Name Synonyms

Secondary FlyBase IDs

References (6)

Report Sections

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