FB2024_03 , released June 25, 2024
Allele: Dmel\BxUAS.Tag:HA
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General Information
Symbol
Dmel\BxUAS.Tag:HA
Species
D. melanogaster
Name
FlyBase ID
FBal0093488
Feature type
allele
Associated gene
Dmel\Bx
Associated Insertion(s)
Carried in Construct
P{UAS-Bx.T:HA1}
Also Known As
UAS-dLMO, UAS-Bx
Key Links
Transgenic product class
wild_type
(Milan et al., 1998)
Mutagen
Nature of the Allele
Transgenic product class
wild_type
(Milan et al., 1998)
Mutagen
in vitro construct
(Milan et al., 1998)
Progenitor genotype
Carried in construct
P{UAS-Bx.T:HA1}
Cytology
Description

UASt regulatory sequences drive expression of Bx tagged at the N-terminus with three copies of Tag:HA.

(Milan et al., 1998)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
Bx
Tagged with
Tag:HA
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Expression of BxUAS.Tag:HA under the control of Scer\GAL4He.PZ reduces the haemocyte count while its expression under the control of Scer\GAL4He.PZ or Scer\GAL4lz-gal4 leads to increased crystal cell numbers when compared to control larvae.

      (Chatterjee et al., 2019)

      Continual expression of BxUAS.Tag:HA under the control of Scer\GAL4mir-263b-Δ-G4 leads to decreased circadian rhythmicity and circadian power under constant dark, as well as a lack of morning anticipation activity under light-dark cycles, as compared to wild-type controls. Adulthood-specific expression under the additional control of Gal80[ts] also leads to a decrease in circadian rhythmicity compared to controls; this phenotype is not clearly induced by expression only during development.

      Continual expression of BxUAS.Tag:HA under the control of Scer\GAL4mir-263b-Δ-G4 disrupts the circadian plasticity of sLNv dorsal projections: while controls exhibit more axon terminal branches at ZT2 than at ZT14 under light-dark cycles or dark conditions, mutants present similar number of axon terminal branches at (presumptive) ZT2 and ZT14, which are similar to ZT14 in controls.

      (Nian et al., 2019)

      BxScer\UAS.T:Ivir\HA1; Scer\GAL4Act5C.PP somatic clones are recovered much less frequently in the dorsal compartment of 3rd instar wing discs than in the ventral compartment. The few surviving dorsal clones adopt more a rounded shape than ventral clones or wild-type control clones.

      (Milan et al., 2002)

      Expression of BxScer\UAS.T:Ivir\HA1 under the control of Scer\GAL4ptc-559.1 results in dorsal wing vein 3 having a ventral identity; vein 3 loses corrugation and the campaniform sensilla that are normally associated with it.

      (Milan et al., 2001)

      Wing discs expressing BxScer\UAS.T:Ivir\HA1 under the control of Scer\GAL4ap-md544 have small wing pouches.

      (Milan and Cohen, 1999)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Suppressed by
      NOT suppressed by
      Statement
      Reference

      BxUAS.Tag:HA, Scer\GAL4ap-md544 has wing disc phenotype, non-suppressible by apUAS.cMa, Scer\GAL4ap-md544

      (Weihe et al., 2001)
      NOT Enhancer of
      NOT Suppressor of
      Other
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      The loss of dorsally located somatic clones of BxScer\UAS.T:Ivir\HA1; Scer\GAL4Act5C.PP cells in late third instar wing discs is suppressed by the following transgenes: BacA\p35Scer\UAS.cHa, trnScer\UAS.cMa, capsScer\UAS.cSa, capsID.Scer\UAS, trnΔC.Scer\UAS.T:Hsap\MYC, fngScer\UAS.B.T:Hsap\MYC, and NECN.Scer\UAS.cGa.

      (Milan et al., 2002)

      Expression of BxScer\UAS.T:Ivir\HA1 under the control of Scer\GAL4ptc-559.1 in a Dlw1/+ background restores campaniform sensilla and corrugation on the dorsal surface of wing vein 3, indicating that these cells have dorsal identity. The wing is overgrown in these flies and an ectopic posterior wing margin is induced in the dorsal surface along the anterior-posterior compartment boundary.

      (Milan et al., 2001)

      Co-expression of apScer\UAS.cMa does not rescue wing pouch growth in wing discs expressing BxScer\UAS.T:Ivir\HA1 under the control of Scer\GAL4ap-md544. Co-expression of ap::BxdLAp.Scer\UAS.T:Zzzz\FLAG rescues wing pouch growth in wing discs expressing BxScer\UAS.T:Ivir\HA1 under the control of Scer\GAL4ap-md544.

      (Weihe et al., 2001)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      BxScer\UAS.T:Ivir\HA1
      BxUAS.T:HA1
      BxUAS.Tag:HA
      Name Synonyms
      Secondary FlyBase IDs
        References (11)