FlyBase Allele Report: Dmel\EcR[31]

General Information

Symbol

Dmel\EcR³¹

Species

D. melanogaster

Name

FlyBase ID

FBal0090695

Feature type

allele

Associated gene

Dmel\EcR

Associated Insertion(s)

P{?lacW}EcR³¹

Carried in Construct

Key Links

Allele class

amorphic allele - genetic evidence

Mutagen

Delta2-3 transposase

Nature of the Allele

Allele class

amorphic allele - genetic evidence

(Choi et al., 2006)

Mutagen

Delta2-3 transposase

(Schubiger et al., 1998)

Progenitor genotype

EcR^k04504

(Schubiger et al., 1998)

Associated Insertion(s)

P{?lacW}EcR³¹

Cytology

Description

Carries 2 deletions of ~1.5kb and ~1kb, separated by 953bp of wild-type EcR-B genomic sequence. A 7bp stretch of the P{lacW}EcR^k04504 element remains.

(Carney et al., 2004)

Imprecise excision of the P{lacW} element, resulting in a 2.5kb deletion.

(Schubiger et al., 1998)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy (with EcR⁹⁹)

(Schubiger et al., 2003)

decreased cell death (with EcR⁹⁹)

(Choi et al., 2006)

decreased cell number | third instar larval stage (with EcR⁹⁹)

(Lee et al., 2019)

lethal (with Df(2R)ED1482)

(Kahsai et al., 2016)

lethal (with EcR⁹⁹)

(Schubiger et al., 2003)

lethal - all die before end of larval stage | recessive

(Schubiger et al., 1998)

partially lethal - majority die (with EcR⁹⁹)

(Choi et al., 2006)

some die during larval stage (with EcR⁹⁹)

(Choi et al., 2006)

some die during larval stage | recessive

(Schubiger et al., 1998)

Phenotype Manifest In

adult brain (with EcR^W53st)

(Lee et al., 2000)

adult mushroom body (with EcR^W53st)

(Lee et al., 2000)

gamma-lobe & neuron (with EcR^W53st)

(Lee et al., 2000)

larval CCAP neuron | third instar larval stage (with EcR⁹⁹)

(Lee et al., 2019)

neuron & ventral nerve cord (with EcR⁹⁹)

(Choi et al., 2006)

Detailed Description

Statement

Reference

EcR³¹/EcR⁹⁹ third instar larvae have slightly fewer Burs-immunoreactive CCAP neurons compared to wild-type controls.

(Lee et al., 2019)

EcR³¹/EcR⁹⁹ mutants (that are mutant for both EcR-B1 amd EcR-B2 isoforms) are developmentally arrested during the course of larval growth, although a few larval escapers develop into prepupae. At the larval stage, in the escapers, approximately 12 Crz-expressing ventral nerve cord neurons are present, while in wild-type they are absent.

Mutants that lack EcR-B1 (e.g. EcR^Q50st/EcR³¹) exhibit no difference in Crz-expressing ventral nerve cord neuronal cell death compared to wild-type.

EcR³¹/EcR⁹⁹ mutants, that lack EcR-B isoforms, exhibit significant differences to EcR^B2.Scer\UAS or EcR^B1.Scer\UAS rescued lines. Complete lack of programmed cell death is observed in 18% of the EcR-B mutants central nervous system, and partial cell death in the remaining 82% of specimens. Overall, approximately 75% of Crz-expressing ventral nerve cord neurons do not undergo programmed cell death in EcR-B mutants. By contrast, expression of EcR^B1.Scer\UAS or EcR^B2.Scer\UAS, under the control of Scer\GAL4^Crz restores complete cell death in 73% or 87% of EcR³¹/EcR⁹⁹ tissue, respectively, while four to eleven neurons are detectable in the remaining specimens.

(Choi et al., 2006)

Late stage EcR⁹⁹/EcR³¹ larvae fail to pupariate normally, but become stationary at the end of the third larval instar and later complete apolysis of their larval cuticle. Pruning of the larval Tv-neurons is inhibited in these animals.

(Schubiger et al., 2003)

In EcR^W53st/EcR³¹ the Mushroom Body (MB) γ neurons retain their larval characteristics including both dorsal and medial lobes and a prominent calyx. Gross abnormalities in brain development are also seen.

(Lee et al., 2000)

Most EcR³¹/EcR^M554fs animals fail to survive to the second larval instar stage and few mutant animals survive to the 3rd larval instar stage. 65-80% of larvae arrested prior to the second instar stage have both 1st and 2nd instar mouth hooks, while those arrested prior to the third instar stage have both 2nd and 3rd instar mouth hooks.

(Schubiger et al., 1998)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhancer of

Statement

Reference

EcR³¹/EcR[+] is a non-enhancer of abnormal neuroanatomy | pupal stage phenotype of Scer\GAL4^ppk.PG, par-1^HMS00405

(Herzmann et al., 2017)

Phenotype Manifest In

NOT Enhancer of

Statement

Reference

EcR³¹/EcR[+] is a non-enhancer of larval multidendritic class IV neuron | pupal stage phenotype of Scer\GAL4^ppk.PG, par-1^HMS00405

(Herzmann et al., 2017)

EcR³¹/EcR[+] is a non-enhancer of dendrite | pupal stage phenotype of Scer\GAL4^ppk.PG, par-1^HMS00405

(Herzmann et al., 2017)

Additional Comments

Genetic Interactions

Statement

Reference

The penetrance of the pruning defects observed in class IV dendritic arborizing neurons of pupae expressing par-1^HMS00405 under the control of Scer\GAL4^ppk.PG is not significantly modified by combination with a single copy of EcR³¹.

(Herzmann et al., 2017)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

EcR⁹⁹/EcR³¹ is rescued by EcR^B1.UAS/Scer\GAL4^Crz.PC

(Choi et al., 2006)

EcR⁹⁹/EcR³¹ is rescued by Scer\GAL4^Crz.PC/EcR^B2.UAS

(Choi et al., 2006)

EcR³¹/EcR^W53st is rescued by Scer\GAL4^Tab2-201Y/EcR^B1.UAS

(Lee et al., 2000)

EcR³¹/EcR^W53st is rescued by Scer\GAL4^Tab2-201Y/EcR^B2.UAS

(Lee et al., 2000)

Partially rescued by

EcR⁹⁹/EcR³¹ is partially rescued by Scer\GAL4^FMRFa.PS/EcR^A.UAS

(Schubiger et al., 2003)

EcR⁹⁹/EcR³¹ is partially rescued by Scer\GAL4^FMRFa.PS/EcR^B2.UAS

(Schubiger et al., 2003)

EcR⁹⁹/EcR³¹ is partially rescued by EcR^B1.UAS/Scer\GAL4^FMRFa.PS

(Schubiger et al., 2003)

Not rescued by

EcR³¹/EcR^W53st is not rescued by Scer\GAL4^Tab2-201Y/EcR^A.UAS

(Lee et al., 2000)

Comments

Expression of EcR^B1.Scer\UAS or EcR^B2.Scer\UAS, under the control of Scer\GAL4^Crz restores complete cell death in 73% or 87% of EcR³¹/EcR⁹⁹ tissue, respectively, while four to eleven neurons are detectable in the remaining specimens.

(Choi et al., 2006)

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

4900

y¹ w¹; EcR³¹/CyO, y⁺

Kyoto

107940

y¹ w¹; EcR³¹/CyO, y⁺

Notes on Origin