FlyBase Allele Report: Dmel\EcR[W53st]

General Information

Symbol

Dmel\EcR^W53st

Species

D. melanogaster

Name

FlyBase ID

FBal0083483

Feature type

allele

Associated gene

Dmel\EcR

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

Mutagen

ethyl methanesulfonate

(Bender et al., 1997)

Progenitor genotype

Cytology

Description

Mutation is within the EcR-B1 specific exon 2.

(Bender et al., 1997)

Nucleotide substitution: G?A.

(Bender et al., 1997)

Amino acid replacement: W53term.

(Bender et al., 1997)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

2R:6,123,403..6,123,403 [-]

Nucleotide change:

G6123403A

Reported nucleotide change:

G?A

Amino acid change:

W53term | EcR-PB; W53term | EcR-PG

Reported amino acid change:

W53term

Comment:

TGG to TAG nonsense mutation at codon W53.

(Bender et al., 1997)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal | recessive

(Bender et al., 1997)

lethal - all die during embryonic stage (with EcR¹²⁰)

(Carney et al., 2004)

partially lethal - majority die (with EcR¹¹)

(Carney et al., 2004)

some die during embryonic stage (with EcR¹²⁰)

(Carney et al., 2004)

some die during pupal stage (with EcR¹¹)

(Carney et al., 2004)

Phenotype Manifest In

adult brain (with EcR³¹)

(Lee et al., 2000)

adult mushroom body (with EcR³¹)

(Lee et al., 2000)

embryonic/larval midgut | embryonic stage

(Bender et al., 1997)

gamma-lobe & neuron (with EcR³¹)

(Lee et al., 2000)

histoblast nest

(Bender et al., 1997)

midgut imaginal island

(Bender et al., 1997)

Detailed Description

Statement

Reference

In EcR^W53st/EcR³¹ the Mushroom Body (MB) γ neurons retain their larval characteristics including both dorsal and medial lobes and a prominent calyx. Gross abnormalities in brain development are also seen.

(Lee et al., 2000)

Some EcR^W53st/EcR^M554fs animals survive to the third larval instar stage, but they do not pupariate. They initiate wandering, but fail to evert spiracles, shorten, attach themselves to a solid surface, or harden their cuticle. The larvae easily slip free of their cuticle when dissected, indicating that apolysis of the larval cuticle has been completed. The imaginal cells of the midgut islands begin to proliferate in EcR^W53st/EcR^M554fs larvae, but they fail to form a tube surrounding the larval epithelial cells, and the epithelial cells fail to become condensed into a compact mass. Proliferation of abdominal histoblasts is initiated in these larvae, but is limited to one or two cell doublings.

(Bender et al., 1997)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

EcR^W53st/EcR[+] is a suppressor of visible phenotype of Pax^UAS.cCa, Scer\GAL4^32B

(Chen et al., 2008)

Phenotype Manifest In