FlyBase Allele Report: Dmel\gbb[D4]

General Information

Symbol

Dmel\gbb^D4

Species

D. melanogaster

Name

FlyBase ID

FBal0090532

Feature type

allele

Associated gene

Dmel\gbb

Associated Insertion(s)

Carried in Construct

Key Links

Allele class

Mutagen

ethyl nitrosourea

Nature of the Allele

Allele class

Mutagen

ethyl nitrosourea

(Chen et al., 1998)

Progenitor genotype

Cytology

Description

1bp deletion, resulting in a frame shift premature stop codon in the prodomain.

(Chen et al., 1998)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

decreased cell number | dominant | progressive

(Pan et al., 2007)

Phenotype Manifest In

Detailed Description

Statement

Reference

gbb^D4 homozygotes do not exhibit any obvious embryonic midgut constriction defects, as compared to controls.

(Galeone et al., 2017)

7-day old gbb^D4/+ germaria have normal numbers of germline stem cells (GSCs), but at 63-days old, these germaria have significantly lower GSC numbers compared to controls - most have only one or no GSCs. 35-day old and 63-day old heterozygous germaria also contain significantly fewer cysts than controls, although cap cells are maintained at normal numbers.

(Pan et al., 2007)

In gbb⁴/gbb^D4 males raised to adulthood at 18^oC and then shifted to 22^oC for 7 days, testes contain pn average 1.3 germline stem cells compared to an average of 9.1 in wild-type. Germline stem cells are eliminated completely after a week at 25^oC.

(Kawase et al., 2004)

In gbb⁴/gbb^D4 females raised to adulthood at 18^oC and then shifted to 29^oC for 7 days, 88% of germaria lack germ cells. Remaining germaria mostly contain only one germ cell.

(Song et al., 2004)

gbb^D4/gbb^D8 larvae have a greatly reduced fat body and appear transparent. The gastric caecae are reduced in length in approximately 50% of larvae. The larvae grow more slowly than wild-type and are somewhat reduced in size. gbb^D4/gbb^D8 embryos fail to form the first midgut constriction, but the second and third constrictions form.

(Chen et al., 1998)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhancer of

Statement

Reference

gbb^D4 is a non-enhancer of increased cell number | adult stage phenotype of mir-958^KO

(Mukherjee et al., 2021)

NOT Suppressor of

Statement

Reference

gbb^D4 is a non-suppressor of increased cell number | adult stage phenotype of mir-958^KO

(Mukherjee et al., 2021)

Other

Statement

Reference

gbb^D4, shn¹ has visible | dominant phenotype

(Chen et al., 1998)

gbb^D4, tkv⁶ has visible | recessive phenotype

(Chen et al., 1998)

gbb^D8/gbb^D4, tkv⁶ has lethal | recessive | embryonic stage phenotype

(Chen et al., 1998)

gbb^D4, tkv⁶ has visible | dominant phenotype

(Chen et al., 1998)

gbb^D4, tkv⁶ has lethal | recessive | embryonic stage phenotype

(Chen et al., 1998)

gbb^D4, put¹³⁵ has visible | dominant phenotype

(Chen et al., 1998)

dpp^s5, gbb^D4 has visible | dominant phenotype

(Chen et al., 1998)

Df(2L)tkv2, gbb^D4 has visible | dominant phenotype

(Chen et al., 1998)

Phenotype Manifest In

Enhanced by

Statement

Reference

gbb^D8/gbb^D4 has embryonic midgut constriction phenotype, enhanceable by tkv⁶

(Chen et al., 1998)

NOT Enhancer of

Statement

Reference

gbb^D4 is a non-enhancer of intestinal stem cell | adult stage | increased number phenotype of mir-958^KO

(Mukherjee et al., 2021)

NOT Suppressor of

Statement

Reference

gbb^D4 is a non-suppressor of intestinal stem cell | increased number | adult stage phenotype of mir-958^KO

(Mukherjee et al., 2021)

Other

Statement

Reference

bam^hs.PO, gbb⁴/gbb^D4 has follicle cell & germarium | conditional - heat sensitive phenotype

(Kirilly et al., 2005)

bam^hs.PO, gbb⁴/gbb^D4 has germarium | heat sensitive phenotype

(Kirilly et al., 2005)

dpp^hr56/dpp^e90, gbb^D4 has male germline stem cell phenotype

(Kawase et al., 2004)

dpp^hr56/dpp^hr4, gbb^D4 has male germline stem cell phenotype

(Kawase et al., 2004)

dpp^hr56/dpp^hr4, gbb[+]/gbb^D4 has male germline stem cell phenotype

(Kawase et al., 2004)

dpp^hr56/dpp^e90, gbb[+]/gbb^D4 has male germline stem cell phenotype

(Kawase et al., 2004)

gbb^D4, tkv⁶ has sex comb | ectopic phenotype

(Chen et al., 1998)

gbb^D4, tkv⁶ has tarsal segment phenotype

(Chen et al., 1998)

gbb^D8/gbb^D4, tkv⁶ has embryo phenotype

(Chen et al., 1998)

gbb^D8/gbb^D4, tkv⁶ has embryonic/first instar larval cuticle phenotype

(Chen et al., 1998)

gbb^D8/gbb^D4, tkv⁶ has embryonic head phenotype

(Chen et al., 1998)

gbb^D4, tkv⁶ has scutum phenotype

(Chen et al., 1998)

gbb^D4, tkv⁶ has leg phenotype

(Chen et al., 1998)

gbb^D4, tkv⁶ has unguis phenotype

(Chen et al., 1998)

Additional Comments

Genetic Interactions

Statement

Reference

bam^hs.PO animals cultured at 18^oC and heat shocked as late stage pupae (37^oC, 4 times for 2 hours each with an 8-12 hour interval) produce adult females with agametic germaria. If these animals also carry the temperature sensitive allelic combination gbb⁴/gbb^D4 and are shifted to 29^oC (the non-permissive temperature) for 10 days, the majority of germaria disintegrate. Among remaining germaria, less than half contain follicle cells but all contain terminal filament cells.

(Kirilly et al., 2005)

gbb^D4 dominantly enhances the tkv⁶ phenotype; tkv⁶ homozygotes have a normal notum and legs, but the presence of one copy of gbb^D4 results in defects in the notum and legs. The most distal tarsal segments and claws are truncated and the ventral lateral sex combs are duplicated. Double heterozygotes with Df(2L)tkv2, dpp^s5, shn¹ or put¹³⁵ may show imaginal disc development defects. No interaction with sax⁵, Mad^P1 or Med⁴ is seen in double heterozygous flies. tkv⁶ gbb^D4/tkv⁶ gbb^D8 embryos form only one midgut constriction. gbb^D4/tkv⁶ gbb^D8 embryos have head defects and an excessive ventral curvature. gbb^D4/tkv⁶ gbb^D8 embryos derived from homozygous tkv⁶ females have a dorsal open phenotype.

(Chen et al., 1998)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

63053

y¹ w^*; P{FRT(w^hs)}G13 sha¹ gbb^D4/SM6a

Notes on Origin

Discoverer

Selected as: Dominant enhancer of the tkv⁶ phenotype. Induced on: tkv⁶.

(Chen et al., 1998)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

(Chen et al., 1998)

Tgfβ-60A^D4

gbb^D4

(Mukherjee et al., 2021, Galeone et al., 2017, Pan et al., 2007, Kirilly et al., 2005)

Name Synonyms

Secondary FlyBase IDs

References (7)

Report Sections

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