FlyBase Allele Report: Dmel\nSyb[ΔF33B]

General Information

Symbol

Dmel\nSyb^ΔF33B

Species

D. melanogaster

Name

FlyBase ID

FBal0088780

Feature type

allele

Associated gene

Dmel\nSyb

Associated Insertion(s)

Carried in Construct

Key Links

Allele class

loss of function allele

Mutagen

Delta2-3 transposase

Nature of the Allele

Allele class

loss of function allele

(Deitcher et al., 1998)

Mutagen

Delta2-3 transposase

(Deitcher et al., 1998)

Progenitor genotype

nSyb^F33

(Deitcher et al., 1998)

Cytology

Description

Imprecise excision of the P{lacW} element, removing much of exon 1 (including the start codon), and all of exons 2, 3 and 4.

(Deitcher et al., 1998)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

neuromuscular junction

(Deitcher et al., 1998)

synapse (with nSyb^I18)

(Stewart et al., 2000)

Detailed Description

Statement

Reference

n-syb¹⁴/n-syb^ΔF33B third instar larvae have a normal number of varicosities at the neuromuscular junction of muscle fibres 6 and 7 and synaptic proliferation at the neuromuscular junction is normal.

(Rieckhof et al., 2003)

Mosaic animals in which the eyes are made homozygous for n-syb^ΔF33B have eyes which are externally indistinguishable from those of controls, however, the on and off transients of the electroretinogram are absent in these flies. No evoked responses are observed at the neuromuscular junction in n-syb^ΔF33B larvae.

(Bhattacharya et al., 2002)

Excitatory synaptic currents (ESCs) are detected in mutant embryos, but virtually no large-amplitude ESCs characteristic of nerve-evoked ESCs are seen. Tetrodotoxin has no effect on the frequency or amplitude of ESCs (in contrast to wild type) and no evoked ESCs are elicited by nerve stimulation.

(Saitoe et al., 2001)

A reduction is seen in EJCs in n-syb^ΔF33B/n-syb^I18 larvae over a full range of Ca²⁺ concentrations. Sensitivity to Calcium is not altered. There are no differences in mEJC amplitude that can explain the observed reduction in synaptic transmission. A reduction in Ca²⁺ cooperativity of synaptic transmission is seen. The mean mEJC decay time is not affected.

(Stewart et al., 2000)

The frequency of miniature excitatory synaptic currents (mESCs) in high K⁺ saline increases in a Ca²⁺-dependent manner in mutant larvae.

(Yoshihara et al., 1999)

The longitudinal tracts of the ventral nervous system, the motor nerves and the terminals of the motor neurons appear normal in homozygous embryos. Stimulation of the ventral ganglion fails to elicit any evoked current in the muscle in homozygous embryos, in contrast to wild-type. The frequency of miniature excitatory synaptic currents at the neuromuscular junction is reduced by approximately 75% in homozygous embryos compared to wild-type, although their mean amplitude is similar to that in wild-type embryos. Transheterozygotes with n-syb^I4 or n-syb^I18 can survive to adulthood, but the flies are very sluggish and often remain motionless for minutes at a time.

(Deitcher et al., 1998)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

nSyb^ΔF33B has abnormal neurophysiology phenotype, suppressible by Syb^hs.PB

(Bhattacharya et al., 2002)

nSyb^ΔF33B has abnormal neurophysiology | somatic clone phenotype, suppressible by Syb^hs.PB

(Bhattacharya et al., 2002)

Phenotype Manifest In

Suppressed by

Statement

Reference

nSyb^ΔF33B has neuromuscular junction phenotype, suppressible by Syb^hs.PB

(Bhattacharya et al., 2002)

Additional Comments

Genetic Interactions

Statement

Reference

Scer\GAL4^elav-C155-driven expression of nSyb^K83A.UAS in the nSyb^ΔF33B background leads to reduced frequency of mESPCs at the larval NMJ compared to the rescue line expressing nSyb^UAS.cLa but the amplitude as well as area of the evoked excitatory postsynaptic currents is comparable in both lines.

(Vasin et al., 2016)

The on and off transients of the electroretinogram are restored in mosaic animals in which the eyes are homozygous for n-syb^ΔF33B if they are also expressing Syb^hs.PB. Evoked responses at the n-syb^ΔF33B neuromuscular junction are partially restored by expression of Syb^hs.PB.

(Bhattacharya et al., 2002)

In Mhc¹ ; n-syb^ΔF33B double mutants, Co²⁺ completely blocks the effect of forskolin on mSC frequency in larval neuromuscular junctions.

(Yoshihara et al., 2000)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

nSyb^ΔF33B is rescued by nSyb^{+tCH322-83G13}

(Venken et al., 2009)

Partially rescued by

nSyb^ΔF33B is partially rescued by nSyb^hs.PB

(Bhattacharya et al., 2002)

Comments

Evoked responses at the n-syb^ΔF33B neuromuscular junction are partially restored by expression of n-syb^hs.PB.

(Bhattacharya et al., 2002)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

51621

y¹ w^*; nSyb^ΔF33B/TM3, P{GAL4-Kr.C}DC2, P{UAS-GFP.S65T}DC10, Sb¹

Notes on Origin

Discoverer

Selected as: Lethal in combination with n-syb^F33.

(Deitcher et al., 1998)

Comments

Shows normal GABA staining in stage 17 nerve cord.

(Kueppers et al., 2003)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (6)

Reported As

Symbol Synonym

n-Syb^ΔF33B

n-syb^ΔF33

(Vasin et al., 2016)

n-syb^ΔF33B

(DeMill et al., 2014, Venken et al., 2009)

n-synaptobrevin^ΔF33B

(Kueppers et al., 2003)

nSyb^ΔF33B

(Yamashita et al., 2022)

nsyb^F33B

(Jin et al., 2018)

Name Synonyms

Secondary FlyBase IDs

References (15)

Report Sections

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