FlyBase Allele Report: Dmel\nSyb[F33]

General Information

Symbol

Dmel\nSyb^F33

Species

D. melanogaster

Name

FlyBase ID

FBal0088778

Feature type

allele

Associated gene

Dmel\nSyb

Associated Insertion(s)

P{lacW}nSyb^F33

Carried in Construct

Also Known As

n-syb^F33-R

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

Delta2-3 transposase

Nature of the Allele

Allele class

Mutagen

Delta2-3 transposase

(Deitcher et al., 1998)

Progenitor genotype

P{lacW}34

(Deitcher et al., 1998)

Associated Insertion(s)

P{lacW}nSyb^F33

Cytology

Description

Insertion of a P{lacW} element in exon 1, 150bp upstream of the ATG codon.

(Deitcher et al., 1998)

Allele components

Component

Use(s)

Inserted element

P{lacW}

enhancer trap

Encoded product / tool

lacZ

reporter enzyme

Tagged with

Tag:NLS(P)

nuclear protein localization tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

3L:1,636,969..1,636,970 [-]

(Deitcher et al., 1998)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

neuromuscular junction

(Deitcher et al., 1998)

Detailed Description

Statement

Reference

The frequency of miniature excitatory synaptic currents (mESCs) in high K⁺ saline increases in a Ca²⁺-dependent manner in mutant larvae.

(Yoshihara et al., 1999)

Stimulation of the ventral ganglion fails to elicit any evoked current in the muscle in homozygous embryos, in contrast to wild-type. The frequency of miniature excitatory synaptic currents at the neuromuscular junction is reduced by approximately 75% in homozygous embryos compared to wild-type, although their mean amplitude is similar to that in wild-type embryos. Transheterozygotes with n-syb^I4 or n-syb^I18 can survive to adulthood, but the flies are very sluggish and often remain motionless for minutes at a time. The flies are also very uncoordinated.

(Deitcher et al., 1998)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhanced by

Statement

Reference

nSyb^F33 has abnormal neuroanatomy phenotype, non-enhanceable by Ranbp11[+]/Impβ11⁷⁰