FlyBase Allele Report: Dmel\apt[tdf-PΔ3]

General Information

Symbol

Dmel\apt^tdf-PΔ3

Species

D. melanogaster

Name

FlyBase ID

FBal0082192

Feature type

allele

Associated gene

Dmel\apt

Associated Insertion(s)

Carried in Construct

Also Known As

apt^PΔ3

Key Links

Allele class

Mutagen

P-element activity

Nature of the Allele

Allele class

Mutagen

P-element activity

(Eulenberg and Schuh, 1997)

Progenitor genotype

apt^tdf-C5-2-5

(Eulenberg and Schuh, 1997)

Cytology

Description

Deletion of the first exon and about 1kb of the first intron.

(Eulenberg and Schuh, 1997)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal cell migration | adult stage | somatic clone

(Starz-Gaiano et al., 2008)

chemical resistant | dominant

(McClure and Heberlein, 2013)

lethal | recessive

(Eulenberg and Schuh, 1997)

Phenotype Manifest In

Detailed Description

Statement

Reference

Heterozygous flies show a dramatic reduction in ethanol sedation sensitivity compared to wild type.

(McClure and Heberlein, 2013)

Egg chambers containing homozygous apt^tdf-PΔ3 mutant clones in stage 10 egg chambers contain 1-7 additional invasive "stretched border cells", often dramatically stretched out and trailing behind the main cluster. This phenotype is observed even if only a few anterior follicle cells are mutant, but no stretched border cells are seen in wild type cells. No egg chambers are seen where the border cells are the only mutant cells.

(Starz-Gaiano et al., 2008)

apt^tdf-PΔ3 homozygous embryos have breakages in the CNS axon tracts and tracheal networks.

(Liu et al., 2003)

Egg chambers of females with homozygous germline clones arrest development in early oogenesis (approximately stage 6) and the oocyte fails to differentiate, with all nuclei becoming polyploid. The egg chambers then degenerate. Some egg chambers have an abnormal number of nuclei.

(Lie and Macdonald, 1999)

Stereotypic outgrowth of tracheal branches is severely disrupted in mutant embryos. Mutants show variable tracheal defects. Embryos fail to perform germ band retraction. Embryos derived from germline clones exhibit a severe and consistent tracheal phenotype: lack of tracheal branch outgrowth and tracheal pit formation. Mutant embryos also show specific defects in the central nervous system, stalled germ band retraction and defects during head formation.

(Eulenberg and Schuh, 1997)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Enhancer of

Statement

Reference

apt^tdf-PΔ3/apt[+] is an enhancer of presumptive embryonic/larval central nervous system phenotype of mbf1²

(Liu et al., 2003)

apt^tdf-PΔ3/apt[+] is an enhancer of presumptive embryonic/larval tracheal system phenotype of mbf1²

(Liu et al., 2003)

Other

Statement

Reference

apt^tdf-PΔ3, bru1^QB has embryonic head phenotype

(Lie and Macdonald, 1999)

apt^tdf-PΔ3, bru1^QB, nanos^L7 has embryonic head phenotype

(Lie and Macdonald, 1999)

Additional Comments

Genetic Interactions

Statement

Reference

Embryos derived from a cross of wild-type males to females heterozygous for aret^QB and also heterozygous for apt^tdf-PΔ3 occasionally have head defects (these defects are not seen in embryos derived from females singly heterozygous for aret^QB or apt^tdf-PΔ3). This phenotype is largely suppressed if the females are also heterozygous for nos^L7.

(Lie and Macdonald, 1999)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

apt^tdf-PΔ3/apt[+] is rescued by apt^UAS.cEa/Scer\GAL4^Crz.PU

(McClure and Heberlein, 2013)

Partially rescued by

apt^tdf-PΔ3 is partially rescued by Scer\GAL4^elav-C155/apt^UAS.cEa

(Liu et al., 2003)

apt^tdf-PΔ3 is partially rescued by apt^UAS.cEa/Scer\GAL4^btl.PS

(Eulenberg and Schuh, 1997)

Comments

Expression of apt^Scer\UAS.cEa under the control of Scer\GAL4^Crz.PU completely rescues the reduced ethanol sedation sensitivity of apt^tdf-PΔ3/+ flies.

(McClure and Heberlein, 2013)

Mutant phenotype can be rescued by Scer\GAL4^btl.PS-mediated expression of apt^Scer\UAS.cEa : tracheal branches grow out and develop the tracheal network including a dorsal trunk. Lack of germ band retraction is not rescued.

(Eulenberg and Schuh, 1997)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

apt^PΔ3

(Liu et al., 2014, McClure and Heberlein, 2013, Starz-Gaiano et al., 2008, Lie and Macdonald, 1999)

apt^tdf-PΔ3

apt^tdfPΔ3

(Starz-Gaiano et al., 2008)

tdf^PΔ3

(Liu et al., 2003)

Name Synonyms

Secondary FlyBase IDs

References (6)

Report Sections

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