FlyBase Allele Report: Dmel\apt[UAS.cEa]

General Information

Symbol

Dmel\apt^UAS.cEa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Eulenberg

FlyBase ID

FBal0082189

Feature type

allele

Associated gene

Dmel\apt

Associated Insertion(s)

Carried in Construct

P{UAS-apt.E}

Also Known As

UAS-apt, UAS-tdf

Key Links

Transgenic product class

cDNA

(Eulenberg and Schuh, 1997)

wild_type

(Eulenberg and Schuh, 1997)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Eulenberg and Schuh, 1997)

wild_type

(Eulenberg and Schuh, 1997)

Mutagen

in vitro construct

(Eulenberg and Schuh, 1997)

Progenitor genotype

Carried in construct

P{UAS-apt.E}

Cytology

Description

UASt regulatory sequences drive expression of the apt coding region from cDNA S3.

(Eulenberg and Schuh, 1997)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

apt

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates carcinoma

modeled by scrib^RNAi.UAS.cUa

(Song et al., 2023)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal cell migration | adult stage, with Scer\GAL4^slbo.2.6

(Starz-Gaiano et al., 2008)

abnormal size | adult stage, with Scer\GAL4^Bx-MS1096

(Wang et al., 2017)

abnormal size | larval stage, with Scer\GAL4^ap-md544

(Wang et al., 2017)

increased cell death | larval stage | somatic clone, with Scer\GAL4^Bx-MS1096

(Wang et al., 2017)

visible, with Scer\GAL4^Bx-MS1096

(Wang et al., 2017)

visible, with Scer\GAL4^sd-SG29.1

(Wang et al., 2017)

Phenotype Manifest In

border follicle cell | oogenesis stage S10, with Scer\GAL4^slbo.2.6

(Starz-Gaiano et al., 2008)

egg chamber | oogenesis stage S10, with Scer\GAL4^slbo.2.6

(Starz-Gaiano et al., 2008)

haltere, with Scer\GAL4^sd-SG29.1

(Wang et al., 2017)

wing, with Scer\GAL4^Bx-MS1096

(Wang et al., 2017)

wing, with Scer\GAL4^sd-SG29.1

(Wang et al., 2017)

wing disc | somatic clone, with Scer\GAL4^Bx-MS1096

(Wang et al., 2017)

wing disc dorsal compartment, with Scer\GAL4^ap-md544

(Wang et al., 2017)

wing margin bristle, with Scer\GAL4^Bx-MS1096

(Wang et al., 2017)

wing vein, with Scer\GAL4^Bx-MS1096

(Wang et al., 2017)

Detailed Description

Statement

Reference

Expression of apt^Scer\UAS.cEa under the control of Scer\GAL4^Bx-MS1096 leads to the formation of severe wing abnormalities in the adult, with very small, blistered wings, disruption of vein patterns and extra abnormal bristles in the wing margin.

Wing disc clones expressing apt^Scer\UAS.cEa under the control of Scer\GAL4^Bx-MS1096 exhibit significantly increased cell death, as compared to controls.

Expression of apt^Scer\UAS.cEa under the control of Scer\GAL4^sd-SG29.1 leads to complete loss of the wings and halteres in adults.

Wing discs expressing apt^Scer\UAS.cEa under the control of Scer\GAL4^ap-md544 exhibit a significant decrease in the size of the dorsal region of the disc, as compared to controls.

(Wang et al., 2017)

Expression of apt^Scer\UAS.cEa under the control of Scer\GAL4^slbo.2.6 results in complete failure of border cell movement in stage 10 egg chambers. The border cells remain at the anterior tip of the egg chamber.

(Starz-Gaiano et al., 2008)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Scer\GAL4^slbo.2.6, apt^UAS.cEa has abnormal cell migration | adult stage phenotype, suppressible by slbo^UAS.cRa/Scer\GAL4^slbo.2.6

(Starz-Gaiano et al., 2008)

Suppressor of

Statement

Reference

apt^UAS.cEa/Scer\GAL4^slbo.2.6 is a suppressor | partially of abnormal cell migration | adult stage phenotype of Scer\GAL4^slbo.2.6, slbo^UAS.cRa

(Starz-Gaiano et al., 2008)

Other

Statement

Reference

BacA\p35^UAS.cHa, Scer\GAL4^ap-md544, apt^UAS.cEa has hyperplasia | larval stage phenotype

(Wang et al., 2017)

Phenotype Manifest In

Suppressed by

Statement

Reference

Scer\GAL4^slbo.2.6, apt^UAS.cEa has egg chamber | oogenesis stage S10 phenotype, suppressible by slbo^UAS.cRa/Scer\GAL4^slbo.2.6

(Starz-Gaiano et al., 2008)

Scer\GAL4^slbo.2.6, apt^UAS.cEa has border follicle cell | oogenesis stage S10 phenotype, suppressible by slbo^UAS.cRa/Scer\GAL4^slbo.2.6

(Starz-Gaiano et al., 2008)

Suppressor of

Statement

Reference

apt^UAS.cEa/Scer\GAL4^slbo.2.6 is a suppressor | partially of egg chamber | oogenesis stage S10 phenotype of Scer\GAL4^slbo.2.6, slbo^UAS.cRa

(Starz-Gaiano et al., 2008)

apt^UAS.cEa/Scer\GAL4^slbo.2.6 is a suppressor | partially of border follicle cell | oogenesis stage S10 phenotype of Scer\GAL4^slbo.2.6, slbo^UAS.cRa

(Starz-Gaiano et al., 2008)

Other

Statement

Reference

BacA\p35^UAS.cHa, Scer\GAL4^ap-md544, apt^UAS.cEa has wing disc phenotype

(Wang et al., 2017)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of slbo^Scer\UAS.cRa suppresses the defects in border cell cluster detachment seen when apt^Scer\UAS.cEa is expressed under the control of Scer\GAL4^slbo.2.6. The border cell cluster invades into the nurse cells. The "stretched border cells" seen when slbo^Scer\UAS.cRa is expressed alone are almost never seen in these flies.

(Starz-Gaiano et al., 2008)

Xenogenetic Interactions

Statement

Reference

Co-expression of apt^Scer\UAS.cEa and BacA\p35^Scer\UAS.cHa under the control of Scer\GAL4^ap-md544 leads to overgrowth of the wing disc, as compared to controls.

(Wang et al., 2017)

Complementation and Rescue Data

Rescues

apt^UAS.cEa/Scer\GAL4^Crz.PU rescues apt^tdf-PΔ3/apt[+]

(McClure and Heberlein, 2013)

Partially rescues

Scer\GAL4^elav-C155/apt^UAS.cEa partially rescues apt^tdf-PΔ3

(Liu et al., 2003)

apt^UAS.cEa/Scer\GAL4^btl.PS partially rescues apt^tdf-PΔ3

(Eulenberg and Schuh, 1997)

Comments

Expression of apt^Scer\UAS.cEa under the control of Scer\GAL4^Crz.PU completely rescues the reduced ethanol sedation sensitivity of apt^tdf-PΔ3/+ flies.

(McClure and Heberlein, 2013)

apt^Scer\UAS.cEa; Scer\GAL4^elav-C155 partially rescues the embryonic trachea phenotype and completely rescues the embryonic CNS phenotype of apt^tdf-PΔ3 homozygotes.

(Liu et al., 2003)

Rescues the tracheal phenotype but not the germ band retraction defect.

(Eulenberg and Schuh, 1997)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

apt^Scer\UAS.cEa

apt^UAS.cEa

tdf^UAS.cEa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Eulenberg

(Eulenberg and Schuh, 1997)

Secondary FlyBase IDs

References (14)

Report Sections

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