P{lacW} insertion in the region between the 3' border of exon 8 and the 5' border of exon 11.
In tutlk14703 embryos, the ISNb motor axons succesfully reach the vicinity of their respective targets. However, once there, many fail to send one or more of the final axon branches to contact their muscle targets. Around a quarter of the hemisegments also lack ISNd nerves.
Approximately 20% of tutlk14703 homozygotes reach adulthood. They exhibit an optic chiasm that is disorganised and exhibits graps in the R7 termination line.
Flies with eyes composed of tutlk14703 mutant cells and brains expressing wild-type levels of tutl (generated using the EGUF technique) exhibit smaller eyes than wild-type, although the optic chiasma and R7 projections appear normal, indicating that the source of retinal axon defects in tutlk14703 mutants is reduced tutl levels in the optic lobe, rather than in the retinal axons.
tutlk14703/tutl23 mutants display a mild R7 tiling phenotype.
Homozygous tutlk14703 embryos show no gastrulation defects.
Most homozygotes are able to survive to adulthood if competition with heterozygous siblings is eliminated. However, competition from heterozygous siblings results in the death of homozygous animals (85-100% lethality) before adulthood. General morphology of the mutant larvae is normal. Homozygous larvae have a severely compromised ability to roll over from an inverted position; the time required to right themselves is significantly longer than control larvae. Homozygous adults cannot fly, although they walk normally and energetically on a level surface. The jump response is intact and robust, but attempts at flight result in the flies flipping themselves onto their backs, where continued efforts to fly are shown by the adults flapping their wings at high speeds (this only results in the flies spinning frantically in circles). Adults that become inverted are rarely able to right themselves, despite what appears to be an intense effort to roll over. Only 12-36% of adults are able to roll over after 60 seconds, in contrast to wild-type which roll over immediately, almost always in less than one second.
tutlk14703 is a suppressor of visible phenotype of HUAS.cMa, Scer\GAL4GMR.PF
tutlk14703/tutl[+] is a suppressor of visible | heat sensitive phenotype of peb1
tutlk14703/tutl[+] is a suppressor of lethal | recessive | partially | embryonic stage phenotype of peb308
tutlk14703 is an enhancer of eye phenotype of Scer\GAL4hs.2sev, mblC.UAS
tutlk14703 is a suppressor of eye phenotype of HUAS.cMa, Scer\GAL4GMR.PF
tutlk14703/tutl[+] is a suppressor of eye | heat sensitive phenotype of peb1
tutl23/tutlk14703 is rescued by tutlRE40452.UAS/Scer\GAL4GMR.long
Expression of either tutlGH15753.Scer\UAS or tutlAT02763.Scer\UAS driven by the pan-neuronal driver Scer\GAL4elav-C155 rescues most aspects of tutlk14703/tutlex383 mutant eye defects.
Expression of tutlScer\UAS.cFa under the control of Scer\GAL4GMR.long rescues the mild tiling phenotype found in tutlk14703/tutl23 R7 terminals.
I. Kiss.
Precise excision of the P{lacW} element rescues the semi-lethality and all the behavioural phenotypes of tutlk14703.