FlyBase Allele Report: Dmel\barr[L305]

General Information

Symbol

Dmel\barr^L305

Species

D. melanogaster

Name

FlyBase ID

FBal0057771

Feature type

allele

Associated gene

Dmel\barr

Associated Insertion(s)

P{?'lacW}barr^L305

Carried in Construct

Key Links

Allele class

amorphic allele - genetic evidence

Mutagen

P-element activity

Nature of the Allele

Allele class

amorphic allele - genetic evidence

(Bhat et al., 1996)

Mutagen

P-element activity

(Bhat et al., 1996)

Progenitor genotype

barr^k00310

(Bhat et al., 1996)

Associated Insertion(s)

P{?'lacW}barr^L305

Cytology

Description

Imprecise excision of P{lacW} insertion.

(Bhat et al., 1996)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

barr^L305 homozygotes and barr^E831/barr^L305 transheterozygotes show variable embryonic muscle pattern defects, which may include: misshaped and disoriented muscles, missing muscles and/or "holes" in the muscle pattern; distorted pharynx musculature; heart cells are occasionally missing or duplicated. These musculature defects are also observed in barr^L305/Df(2L)Exel7077 transheterozygotes that also express barr^{UASp.cOa.EGFP} under the control of either Scer\GAL4^sns.PK or Scer\GAL4^{kirre-rP298-G4}, but not when under the control of either Scer\GAL4^twi.PB or Scer\GAL4^Mef2.PR.

(Jacobs, 2018.7.9)

Average Citrate Synthase activity is increased by 4% in heterozygous males

(Chen et al., 2008)

Homozygous embryos exhibit severe loss of PNS neurons and a significant increase in size of remaining neurons. Embryos arrest in cycle 16, in the majority of anaphase figures chromatids do not resolve but remain interlocked to form extensive chromatin bridges. The unresolved chromatids decondense without segregation and cytokinesis is attempted. Trl staining reveals mutation does not prevent centromere separation.

(Bhat et al., 1996)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT suppressed by

Statement

Reference

barr^L305 has enhancer of variegation phenotype, non-suppressible by glu^88-82

(Cobbe et al., 2006)

NOT Suppressor of

Statement

Reference

barr^L305 is a non-suppressor of suppressor of variegation phenotype of In(1)w^m4, glu^88-82

(Cobbe et al., 2006)

Other

Statement

Reference

Df(3L)BSC395, barr^L305 has decreased cell number | embryonic stage phenotype

(Jacobs, 2018.7.9)

Phenotype Manifest In

NOT Enhanced by

Statement

Reference

barr^L305 has abdominal segment 6 phenotype, non-enhanceable by Abd-B^Fab7-1/Abd-B[+]

(Lupo et al., 2001)

NOT suppressed by

Statement

Reference

barr^L305 has abdominal segment 6 phenotype, non-suppressible by Abd-B^Fab7-1/Abd-B[+]

(Lupo et al., 2001)

Enhancer of

Statement

Reference

barr[+]/barr^L305 is an enhancer of abdominal segment 6 phenotype of Abd-B^Fab7-2

(Lupo et al., 2001)

NOT Enhancer of

Statement

Reference

barr[+]/barr^L305 is a non-enhancer of abdominal segment 6 phenotype of Abd-B^Fab7-1

(Lupo et al., 2001)

NOT Suppressor of

Statement

Reference

barr[+]/barr^L305 is a non-suppressor of abdominal segment 6 phenotype of Abd-B^Fab7-1

(Lupo et al., 2001)

Other

Statement

Reference

Df(3L)BSC395, barr^L305 has embryonic/larval muscle system | embryonic stage phenotype

(Jacobs, 2018.7.9)

Additional Comments

Genetic Interactions

Statement

Reference

barr^L305, Df(3L)BSC395 double homozygous embryos show disorganized somatic muscles, which are drastically reduced in mass and number, as compared to controls.

(Jacobs, 2018.7.9)

Intermediate eye pigment levels are observed in glu^88-82, barr^L305 double mutants with a In(1)w^m4h background, showing that glu^88-82 suppressor effects of PEV are not dominant over the enhancer activity of barr^L305.

(Cobbe et al., 2006)

Chromatin condenses nonuniformly in prophase and prometaphase in postblastoderm Cap-G^K1 barr^L305 double mutant embryos. By metaphase, chromosome condensation has reached wild-type levels. Chromatid separation defects are seen in anaphase when only the fourth chromosome is clearly seen to separate, and lagging chromosomes are seen in telophase. The double mutant postblastoderm embryos have an increased number of prometaphase figures compared to wild-type embryos, suggesting that the length of prometaphase may be increased in the mutant embryos.

(Dej et al., 2004)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

barr^L305/Df(2L)Exel7077 is rescued by Scer\GAL4^da.G32/barr^{UASp.cOa.EGFP}

(Oliveira et al., 2007)

barr^L305/Df(2L)Exel7077 is rescued by Scer\GAL4^Tub.PU/barr^{UASp.cOa.EGFP}

(Oliveira et al., 2007)

barr^L305 is rescued by barr^hs.PB

(Bhat et al., 1996)