FlyBase Allele Report: Dmel\spi[SCP2]

General Information

Symbol

Dmel\spi^SCP2

Species

D. melanogaster

Name

FlyBase ID

FBal0056118

Feature type

allele

Associated gene

Dmel\spi

Associated Insertion(s)

P{PZ}spi^SCP2

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

loss of function allele

Mutagen

P-element activity

Nature of the Allele

Allele class

loss of function allele

(Tio et al., 1994)

Mutagen

P-element activity

(Tio et al., 1994)

Progenitor genotype

Associated Insertion(s)

P{PZ}spi^SCP2

Cytology

Description

P{PZ} insertion within 5' flanking sequences.

(Tio et al., 1994)

Allele components

Component

Use(s)

Inserted element

P{PZ}

enhancer trap

Encoded product / tool

lacZ

reporter enzyme

Tagged with

Tag:NLS(P)

nuclear protein localization tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

2L:19,575,578..19,583,783

(Yu et al., 2010, Brown and Freeman, 2003, Tio et al., 1994)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

partially lethal

(Tio et al., 1994)

viable

(Tio et al., 1994)

visible

(Freeman, 1996, Tio et al., 1994)

Phenotype Manifest In

egg | dorsal

(Wasserman and Freeman, 1998)

eye

(Freeman, 1996)

retina & basal lamina

(Tio et al., 1994)

rhabdomere

(Tio et al., 1994)

Detailed Description

Statement

Reference

spi^SCP2/spi¹ hypomorphic females lay eggs that show a significant loss of the most dorsal tissue. Germline clones reveal no requirement for spi in the oocyte in patterning the egg, or in the viability or patterning of the embryo.

(Wasserman and Freeman, 1998)

73% homozygotes are viable. Homozygotes exhibit a rough eye phenotype, ommatidia have variable and reduced numbers of rhabdomeres and defects in the retinal basement membrane (cells with rhabdomeres appear below the retina). Hemizygotes exhibit enhanced rough eye phenotype and reduced viability.

(Tio et al., 1994)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Null alleles of ru interact dominantly with spi^SCP1 and spi^SCP2.

(Wasserman et al., 2000)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

spi¹/spi^SCP2 is rescued by Scer\GAL4^GMR.PF/spi^UAS.cSa/Scer\GAL4^hs.PB

(Huang et al., 1998)

spi¹/spi^SCP2 is rescued by Scer\GAL4^αTub84B.PP/spi^UAS.cSa/Scer\GAL4^hs.PB

(Huang et al., 1998)

spi^SCP2/spi² is rescued by Scer\GAL4^GMR.PF/spi^UAS.cSa/Scer\GAL4^hs.PB

(Huang et al., 1998)

spi^SCP2/spi² is rescued by Scer\GAL4^αTub84B.PP/spi^UAS.cSa/Scer\GAL4^hs.PB

(Huang et al., 1998)

spi^SCP2/spi³ is rescued by Scer\GAL4^GMR.PF/spi^UAS.cSa/Scer\GAL4^hs.PB

(Huang et al., 1998)

spi^SCP2/spi³ is rescued by Scer\GAL4^αTub84B.PP/spi^UAS.cSa/Scer\GAL4^hs.PB

(Huang et al., 1998)

spi^SCP2 is rescued by spi^s.sev

(Freeman, 1996)

Partially rescued by

spi¹/spi^SCP2 is partially rescued by spi^UAS.cSa/Scer\GAL4^hs.PB

(Huang et al., 1998)

spi^SCP2/spi² is partially rescued by spi^UAS.cSa/Scer\GAL4^hs.PB

(Huang et al., 1998)

spi^SCP2/spi³ is partially rescued by spi^UAS.cSa/Scer\GAL4^hs.PB

(Huang et al., 1998)

Comments

Animals heterozygous for spi^SCP2 and spi¹, spi² or spi³ which have been rescued by spi^Scer\UAS.cSa with Scer\GAL4^hs.PB to the third instar stage show relatively normal ommatidial development at the posterior of the eye imaginal disc. More anterior columns of ommatidia have reduced photoreceptors. In the lamina, retinal projections appeared normal but LPCs show no neuronal differentiation. This phenotype is rescued by the introduction of Scer\GAL4^GMR.PF or in clones induced to produce Scer\GAL4^αTub84B.PP.

(Huang et al., 1998)

spi^s.sev can rescue the eye specific mutation.

(Freeman, 1996)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

spi^SCP2

spi^scp2

(Brown and Freeman, 2003, Wasserman et al., 2000)

Name Synonyms

Secondary FlyBase IDs

References (8)

Report Sections

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