FlyBase Allele Report: Dmel\glu[k08819]

General Information

Symbol

Dmel\glu^k08819

Species

D. melanogaster

Name

FlyBase ID

FBal0043098

Feature type

allele

Associated gene

Dmel\glu

Associated Insertion(s)

P{lacW}glu^k08819

Carried in Construct

Also Known As

glu¹, l(2)k08819, SMC4^k08819

Key Links

Genomic Maps

JBrowse

Allele class

loss of function allele

Mutagen

P-element activity

Nature of the Allele

Allele class

loss of function allele

(Steffensen et al., 2001)

Mutagen

P-element activity

(Kania et al., 1995)

Progenitor genotype

Associated Insertion(s)

P{lacW}glu^k08819

Cytology

Description

A P{lacW} element is inserted upstream of nucleotides 352-375, which correspond to the Walker A motif residues 118-125 in the expressed product. There are stop codons in all reading frames at the 5' end of the insertion, meaning that a 122-residue truncated protein could be produced.

(Cobbe et al., 2006)

P{lacW} insertion in the glu coding sequence.

(Prokopenko et al., 2000)

Insertion of a P{lacW} element.

(Kania et al., 1995)

Allele components

Component

Use(s)

Inserted element

P{lacW}

enhancer trap

Encoded product / tool

lacZ

reporter enzyme

Tagged with

Tag:NLS(P)

nuclear protein localization tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

2L:16,737,930..16,737,930 [+]

(Cobbe et al., 2006, Beaton, 2000.1.31, Prokopenko et al., 2000, Cobbe et al., 1999, Spradling et al., 1999, Kania et al., 1995, BDGP Project Members, 1994-1999)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

adult brain (with glu^k06821a)

(Steffensen et al., 2001)

mitosis & nuclear chromosome (with glu^k06821a)

(Steffensen et al., 2001)

mitotic cell cycle (with glu^k06821a)

(Steffensen et al., 2001)

mitotic domain & mitotic anaphase

(Cobbe et al., 1999)

presumptive embryonic/larval peripheral nervous system

(Kania et al., 1995)

Detailed Description

Statement

Reference

glu^k08819 mutant embryos have a lower ratio of mitosis to apoptosis compared to wild-type embryos. This ratio decreases further with age.

(Cobbe et al., 2006)

About 9/10 of prophases or metaphases show abnormal condensation. About 4/5 of glu^k06821a/glu^k08819 anaphase or telophase figures show chromatin bridges, those bridges that persist to telophase also show abnormal decondensation. Mutant brains are smaller and show higher levels of apoptosis than wild-type. 2.5% of cells in glu^k06821a/glu^k08819 brains are apoptotic (compared to 0.5% in wild-type). Mean chromosome length in glu^k06821a/glu^k08819 brains that have been incubated in colchicine does not differ from wild-type.

(Steffensen et al., 2001)

Embryos show anaphase bridges in mitotic domains.

(Cobbe et al., 1999)

Ventral and lateral PNS of homozygous embryos exhibit subtle organisation defects resulting in irregularly shaped clusters.

(Kania et al., 1995)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

glu[+]/glu^k08819 is an enhancer of male semi-fertile phenotype of Cap-H2^Z3-5163/Cap-H2^Z3-0019

(Hartl et al., 2008)

Other

Statement

Reference

glu^k08819, pros¹⁷ has abnormal mitotic cell cycle phenotype

(Cobbe et al., 2006)

Phenotype Manifest In

Enhanced by

Statement

Reference

Cap-H2^Z3-0019, glu[+]/glu^k08819 has polytene chromosome phenotype, enhanceable by slmb[+]/slmb^UU11

(Buster et al., 2013)

Cap-H2^Z3-0019, glu[+]/glu^k08819 has nurse cell phenotype, enhanceable by slmb[+]/slmb^UU11

(Buster et al., 2013)

Cap-H2^Z3-0019, glu[+]/glu^k08819 has polytene chromosome phenotype, enhanceable by slmb^3A1/slmb[+]

(Buster et al., 2013)

Cap-H2^Z3-0019, glu[+]/glu^k08819 has nurse cell phenotype, enhanceable by slmb^3A1/slmb[+]

(Buster et al., 2013)

Other

Statement

Reference

Cap-H2^Z3-0019, glu[+]/glu^k08819 has nurse cell phenotype

(Buster et al., 2013)

Cap-H2^Z3-0019, glu[+]/glu^k08819 has polytene chromosome phenotype

(Buster et al., 2013)

Cap-H2^Z3-0019, glu[+]/glu^k08819 has polytene chromosome & nurse cell phenotype

(Hartl et al., 2008)

glu^k08819, pros¹⁷ has mitosis & nuclear chromosome phenotype

(Cobbe et al., 2006)

Additional Comments

Genetic Interactions

Statement

Reference

glu^k08819/+ ; Cap-H2^Z3-0019/+ double heterozygotes show some unpairing of polytene chromosomes in nurse cells. Unpairing is significantly increased if the animals are also heterozygous for either slmb^UU11 or slmb^3A1.

(Buster et al., 2013)

A glu^k08819 heterozygous background lead to a substantial decrease in fertility for Cap-H2^Z3-5163/Cap-H2^Z3-0019 mutants.

(Hartl et al., 2008)

77.3 +/- 9.1% of the nurse cells in stage 10 egg chambers of glu^k08819/+ ; Cap-H2^Z3-0019/+ double heterozygous females show persistence of polyteny, with the chromosomes having a loosened, but clear, polytene morphology.

(Hartl et al., 2008)

glu^k08819; pros¹⁷ mitotic cells accumulate in prophase/premetaphase. These cells delay in metaphase and display a high frequency of anaphase and telophase bridges.

(Cobbe et al., 2006)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Complements

glu^k06821a

(BDGP Project Members, 1994-1999)

Fails to complement

glu^k06821a

(BDGP Project Members, 1994-1999)

Comments

Images (0)

Mutant

Wild-type

Stocks (3)

Bloomington

10831

y¹ w^67c23; P{lacW}glu^k08819/CyO

Kyoto

108860

y¹ w^67c23; P{lacW}glu^k08819/CyO

102672

y¹ w^67c23 ; P{lacW}glu^k08819 / CyO

Notes on Origin

Discoverer

I. Kiss.

Comments

After six generations of outcrossing the lethality continued to segragate with the P{lacW} insertion.

(Beaton, 2000.1.31)

Complements: Cas^k03902. Complements: Mhc^k10423. Complements: l(2)k13905^k13905. Complements: l(2)k15102^k15102. Complements: l(2)k16215^k16215.

(BDGP Project Members, 1994-1999)

Lethality not revertible with excision of the P{lacW} insert, but lethality over Df(2L)H20 suggests the insertion may be the cause of the phenotype.

(Kania et al., 1995)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (8)

Reported As

Symbol Synonym

88/19

(Kania et al., 1995)

SMC4⁰⁰⁸⁸¹⁹

(Hartl et al., 2008)

SMC4^k08819

(Hartl et al., 2008, Hartl et al., 2008)

Smc4^k08819

(Buster et al., 2013)

glu1

(Steffensen et al., 1999)

glu¹

(Cobbe et al., 2006, Steffensen et al., 2001)

glu^k08819

(Bivik et al., 2015, Chanut-Delalande et al., 2007, Christensen et al., 2007.10.29)

l(2)k08819

(Prokopenko et al., 2000, Spradling et al., 1999)

Name Synonyms

Secondary FlyBase IDs

FBal0100364

References (18)

Report Sections

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