FlyBase Allele Report: Dmel\aop[ACT.UAS]

General Information

Symbol

Dmel\aop^ACT.UAS

Species

D. melanogaster

Name

activated

FlyBase ID

FBal0042850

Feature type

allele

Associated gene

Dmel\aop

Associated Insertion(s)

Carried in Construct

P{UAS-aop.ACT}

Also Known As

UAS-yan^ACT, UAS-aop^ACT, UAS-aop.ACT

Key Links

Transgenic product class

missense_variant

(Rebay and Rubin, 1995)

polypeptide_post_translational_processing_variant

(Rebay and Rubin, 1995)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

missense_variant

(Rebay and Rubin, 1995)

polypeptide_post_translational_processing_variant

(Rebay and Rubin, 1995)

Mutagen

in vitro construct

(O'Keefe et al., 1997, Shishido et al., 1997, Rebay and Rubin, 1995)

Progenitor genotype

aop^UAS.cRa

(Rebay and Rubin, 1995)

Carried in construct

P{UAS-aop.ACT}

Cytology

Description

A constitutively active form of aop is expressed under the control of UAS regulatory sequences.

(O'Keefe et al., 1997)

An activated form of aop is expressed under the control of UAS regulatory sequences.

(Shishido et al., 1997)

UAS regulatory sequences drive expression of aop in which phosphoacceptor residues at the eight consensus sites within the coding region are replaced with a nonphosphorylatable amino acid, alanine.

(Rebay and Rubin, 1995)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

aop

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy, with Scer\GAL4^elav-C155

(Tootle et al., 2003)

abnormal neuroanatomy | embryonic stage, with Scer\GAL4^repo

(Sepp and Auld, 2003)

decreased cell number | embryonic stage, with Scer\GAL4^tin.CΔ4, Scer\GAL4^tin.cBa

(Schwarz et al., 2018)

increased cell death, with Scer\GAL4^hs.PB

(Kurada and White, 1998)

increased cell number | larval stage, with Scer\GAL4^He.PZ

(Zettervall et al., 2004)

increased cell number | spermatogenesis, with Scer\GAL4^NP1624

(Feng et al., 2017)

lethal, with Scer\GAL4^dpp.blk1

(Wessells et al., 1999)

lethal - all die during embryonic stage, with Scer\GAL4^69B

(Riesgo-Escovar and Hafen, 1997)

lethal - all die during embryonic stage, with Scer\GAL4^elav-C155

(Rebay and Rubin, 1995)

long lived | RU486 conditional, with Scer\GAL4^Switch1.106

(Slack et al., 2015, Alic et al., 2014)

neoplasia | spermatogenesis, with Scer\GAL4^NP1624

(Feng et al., 2017)

partially lethal - majority die, with Scer\GAL4^GMR.PF

(Corl et al., 2009)

short lived, with Scer\GAL4^Switch1.106

(Martínez Corrales et al., 2022)

some die during embryonic stage, with Scer\GAL4^69B

(Riesgo-Escovar and Hafen, 1997)

some die during embryonic stage, with Scer\GAL4^elav-C155

(Rebay and Rubin, 1995)

visible, with Scer\GAL4^dpp.blk1

(Wessells et al., 1999)

visible, with Scer\GAL4^lz-gal4

(Behan et al., 2002)

Phenotype Manifest In

border follicle cell, with Scer\GAL4^slbo.2.6

(Schober et al., 2005)

cardiogenic mesoderm, with Scer\GAL4^how-24B

(Bidet et al., 2003)

cyst progenitor cell | increased number, with Scer\GAL4^NP1624

(Feng et al., 2017)

denticle row 1, with Scer\GAL4^en-e16E

(O'Keefe et al., 1997)

embryo, with Scer\GAL4^hs.PB

(Kurada and White, 1998)

embryonic/larval crystal cell, with Scer\GAL4^He.PZ

(Zettervall et al., 2004)

embryonic/larval dorsal vessel | embryonic stage, with Scer\GAL4^htl.POS

(Shishido et al., 1997)

embryonic/larval ganglionic tracheal branch, with Scer\GAL4^bs-23.26

(Gallio et al., 2004)

embryonic/larval somatic muscle cell, with Scer\GAL4^htl.POS

(Shishido et al., 1997)

embryonic cardioblast, with Scer\GAL4^tin.CΔ4, Scer\GAL4^tin.cBa

(Schwarz et al., 2018)

embryonic dorsal epidermis, with Scer\GAL4^69B

(Riesgo-Escovar and Hafen, 1997)

embryonic ostial cardioblast, with Scer\GAL4^tin.CΔ4, Scer\GAL4^tin.cBa

(Schwarz et al., 2018)

eye, with Scer\GAL4^lz-gal4

(Behan et al., 2002)

eye disc morphogenetic furrow, with Scer\GAL4^dpp.blk1

(Kumar and Moses, 2001)

fascicle | embryonic stage, with Scer\GAL4^repo

(Sepp and Auld, 2003)

heart primordium, with Scer\GAL4^tin.CΔ4, Scer\GAL4^tin.cBa

(Schwarz et al., 2018)

hemocyte, with Scer\GAL4^He.PZ

(Zettervall et al., 2004)

lamellocyte, with Scer\GAL4^He.PZ

(Zettervall et al., 2004)

larval longitudinal connective, with Scer\GAL4^sim.PS

(Scholz et al., 1997, Scholz et al., 1997)

macrochaeta, with Scer\GAL4^dpp.blk1

(Wessells et al., 1999)

male germline cell | increased number, with Scer\GAL4^NP1624

(Feng et al., 2017)

male germline stem cell | increased number, with Scer\GAL4^NP1624

(Feng et al., 2017)

melanotic mass, with Scer\GAL4^He.PZ

(Zettervall et al., 2004)

mesoderm, with Scer\GAL4^twi.PG

(Halfon et al., 2000)

motor neuron | embryonic stage, with Scer\GAL4^repo

(Sepp and Auld, 2003)

muscle founder cell, with Scer\GAL4¹¹⁵¹

(Dutta et al., 2005)

presumptive embryonic/larval central nervous system, with Scer\GAL4^elav-C155

(Tootle et al., 2003)

presumptive embryonic/larval peripheral nervous system, with Scer\GAL4^repo

(Sepp and Auld, 2003)

segment border muscle cell, with Scer\GAL4^how-24B

(Bidet et al., 2003)

sensory neuron & axon & embryo, with Scer\GAL4^repo

(Sepp and Auld, 2003)

spermatogonial cyst, with Scer\GAL4^NP1624

(Feng et al., 2017)

symmetrical commissure, with Scer\GAL4^sim.PS

(Scholz et al., 1997, Scholz et al., 1997)

symmetrical commissure, with Scer\GAL4^sli.PS

(Scholz et al., 1997, Scholz et al., 1997)

terminal tracheal cell, with Scer\GAL4^btl.PS

(Caviglia and Luschnig, 2013)

tracheal fusion cell, with Scer\GAL4^btl.PS

(Caviglia and Luschnig, 2013)

visceral muscle fiber, with Scer\GAL4¹¹⁵¹

(Dutta et al., 2005)

wing disc, with Scer\GAL4^dpp.blk1

(Wessells et al., 1999)

Detailed Description

Statement

Reference

Embryos expressing aop^ACT.UAS under the double control of Scer\GAL4^tin.cBa and Scer\GAL4^tin.CΔ4 leads to a severe decrease in the overall number of cardioblasts (both generic and ostial cardioblasts), as compared to controls.

(Schwarz et al., 2018)

The expression of aop^ACT.UAS under the control of Scer\GAL4^NP1624 leads to testes showing increased numbers of cyst stem cells (Zfh-1-positive cells), accompanied by structures that resemble stem cell, GB and spermatogonial tumors.

(Feng et al., 2017)

Expression of aop^ACT.Scer\UAS under the control of Scer\GAL4^Switch1.106 (and RU486 treatment) has no effect on lifespan compared to controls.

(Slack et al., 2015)

Expression of aop^ACT.Scer\UAS in the adult gut and fat body under the control of Scer\GAL4^Switch1.106 (in the presence of RU486) results in significant lifespan extension. No extension of lifespan is seen when aop^ACT.Scer\UAS is expressed solely in the gut under the control of Scer\GAL4^TIGS-2.

(Alic et al., 2014)

Expression of aop^ACT.Scer\UAS under the control of Scer\GAL4^btl.PS inhibits specification of tracheal fusion cells and terminal cells at every branch tip.

(Caviglia and Luschnig, 2013)

Expression of aop^ACT.Scer\UAS under the control of Scer\GAL4¹¹⁵¹ results in no muscle fibers being formed and no founders being selected.

(Dutta et al., 2005)

Overexpression of aop^ACT.Scer\UAS in outer border cells, driven by Scer\GAL4^slbo.2.6, results in a strong delay in border cell migration; 60% migrate less than 25%, 26% migrate 25-50%, 12% migrate 50-75% and 2% complete their migration.

(Schober et al., 2005)

Migration of many of the ganglionic tracheal branches is stalled or misrouted in aop^ACT.Scer\UAS; Scer\GAL4^bs-23.26 embryos, but branches do not cross the ventral midline.

(Gallio et al., 2004)

When expression is driven by Scer\GAL4^He.PZ, hemocyte proliferation is increased, lamellocyte numbers are increased and crystal cells numbers are increased. Melanotic masses form.

(Zettervall et al., 2004)

Embryos expressing aop^ACT.Scer\UAS under the control of Scer\GAL4^how-24B show loss of lateral adult muscle precursors and a dramatic reduction in precursors of the segmental border muscle. Embryos expressing aop^ACT.Scer\UAS under the control of Scer\GAL4^how-24B show marked loss of cardiac cells in some segments.

(Bidet et al., 2003)

Expression of aop^ACT.Scer\UAS in embryonic glia, under the control of Scer\GAL4^repo, causes PNS patterning defects in embryonic hemisegments. In hemisegments that are moderately affected, sensory axons can be misdirected and nerves in the CNS-PNS transition zone show incorrect fasciculation-bundling. In severely-affected hemisegments, there is a loss of sensory neurons and those remaining have misplaced cell bodies and misdirected sensory axons. Additionally, motor neuron patterning is disrupted.

(Sepp and Auld, 2003)

Central nervous system development is inhibited in embryos expressing aop^ACT.Scer\UAS under the control of Scer\GAL4^elav-C155.

(Tootle et al., 2003)

Flies expressing aop^ACT.Scer\UAS under the control of Scer\GAL4^lz-gal4 have an eye phenotype which resembles lz null flies.

(Behan et al., 2002)

Expression of aop^ACT.Scer\UAS under the control of Scer\GAL4^dpp.blk1 affects furrow reincarnation but not birth in the eye disc.

(Kumar and Moses, 2001)

Expression of aop^ACT.Scer\UAS under the control of Scer\GAL4^twi.PG inhibits eve-expressing progenitor formation in the mesoderm.

(Halfon et al., 2000)

Expression of aop^ACT.Scer\UAS driven by Scer\GAL4^dpp.blk1 leads to abnormal wing discs with a central cleft. In the few adult escapers that survive, a loss of macrochaetae on the scutellum is seen, and wings are small and sometimes bifurcated.

(Wessells et al., 1999)

When expression is driven by Scer\GAL4^hs.PB, extra cell death is induced.

(Kurada and White, 1998)

Embryos expressing aop^ACT.Scer\UAS under the control of Scer\GAL4^en-e16E are missing the normal first denticle row of each belt.

(O'Keefe et al., 1997)

Scer\GAL4^69B-mediated expression during embryogenesis prevents dorsal closure and results in dead embryos with a strong dorsal open phenotype.

(Riesgo-Escovar and Hafen, 1997)

Scer\GAL4^sim.PS-mediated expression leads to fused commissures and to disruption in the longitudinal axons. Scer\GAL4^sim.PS-mediated expression leads to moderate fusion of the segmental commissures.

(Scholz et al., 1997, Scholz et al., 1997)

Heart and somatic muscle formation is deranged in embryos expressing aop^ACT.Scer\UAS under the control of Scer\GAL4^htl.POS.

(Shishido et al., 1997)

Embryos fail to complete germ band retraction.

(Rebay and Rubin, 1995)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Scer\GAL4^GMR.PF, aop^ACT.UAS has partially lethal - majority die phenotype, enhanceable by hppy^RB.UAS/Scer\GAL4^GMR.PF

(Corl et al., 2009)

NOT Enhanced by

Statement

Reference

Scer\GAL4^Switch1.106, aop^ACT.UAS has long lived | RU486 conditional phenotype, non-enhanceable by Ras85D^N17.UAS, Scer\GAL4^Switch1.106

(Slack et al., 2015)

Scer\GAL4^Switch1.106, aop^ACT.UAS has long lived | RU486 conditional phenotype, non-enhanceable by chico¹/chico[+]

(Slack et al., 2015)

NOT suppressed by

Statement

Reference

Scer\GAL4^Switch1.106, aop^ACT.UAS has long lived | RU486 conditional phenotype, non-suppressible by Ras85D^N17.UAS, Scer\GAL4^Switch1.106

(Slack et al., 2015)

Scer\GAL4^Switch1.106, aop^ACT.UAS has long lived | RU486 conditional phenotype, non-suppressible by foxo^unspecified/foxo^unspecified

(Alic et al., 2014)

NOT Enhancer of

Statement

Reference

aop^ACT.UAS/Scer\GAL4^Switch1.106 is a non-enhancer of long lived | dominant phenotype of chico¹

(Slack et al., 2015)

Suppressor of

Statement

Reference

aop^ACT.UAS/Scer\GAL4^Switch1.106 is a suppressor of short lived phenotype of Scer\GAL4^Switch1.106, foxo^UAS.cFa, pnt^P1.UAS

(Alic et al., 2014)

Scer\GAL4^TIGS-2/aop^ACT.UAS is a suppressor | partially of short lived phenotype of Scer\GAL4^TIGS-2, foxo^UAS.cFa, pnt^P1.UAS

(Alic et al., 2014)

Phenotype Manifest In

Suppressed by

Statement

Reference

Scer\GAL4^slbo.2.6, aop^ACT.UAS has border follicle cell phenotype, suppressible | partially by shg^UAS.cOa/Scer\GAL4^slbo.2.6

(Schober et al., 2005)

Enhancer of

Statement

Reference

aop^ACT.UAS/Scer\GAL4^slbo.2.6 is an enhancer of border follicle cell phenotype of slbo⁰¹³¹⁰

(Schober et al., 2005)

Suppressor of

Statement

Reference

Scer\GAL4^69B/aop^ACT.UAS is a suppressor of embryonic/first instar larval cuticle | ventral phenotype of raw¹

(Byars et al., 1999)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of foxo^Scer\UAS.cFa enhances the reduction in lifespan seen when pnt^P1.Scer\UAS is expressed in the adult midgut and fat body under the control of Scer\GAL4^Switch1.106 (in the presence of RU486). This phenotype is completely suppressed upon expression of aop^ACT.Scer\UAS. Co-expression of foxo^Scer\UAS.cFa and pnt^P1.Scer\UAS also enhances the reduction in stored triacylglycerol (TAG) levels seen when either transgene is expressed alone. Gut integrity and feeding appear normal.

Expression of foxo^Scer\UAS.cFa enhances the reduction in lifespan seen when pnt^P1.Scer\UAS is expressed in the adult midgut under the control of Scer\GAL4^TIGS-2 (in the presence of RU486). This phenotype is partially suppressed upon expression of aop^ACT.Scer\UAS.

Flies co-expressing pnt^P1.Scer\UAS and foxo^Scer\UAS.cFa under the control of Scer\GAL4^Switch1.106 are starvation sensitive after 5 days in the presence of RU486, and this phenotype can be reversed upon expression of aop^ACT.Scer\UAS.

Homozygous foxo^unspecified does not suppress the increase in lifespan seen when aop^ACT.Scer\UAS is expressed in the adult gut and fat body under the control of Scer\GAL4^Switch1.106.

(Alic et al., 2014)

Coexpression of shg^Scer\UAS.cOa partially rescues the border cell migration defect of Scer\GAL4^slbo.2.6>aop^ACT.Scer\UAS egg chambers. Expression of aop^ACT.Scer\UAS, driven by Scer\GAL4^slbo.2.6, strongly enhances border cell migration defects in slbo⁰¹³¹⁰ egg chambers.

(Schober et al., 2005)

The ventral cuticle in raw¹ mutant embryos expressing aop^ACT.Scer\UAS under the control of Scer\GAL4^69B develops normally, as it does in embryos expressing aop^ACT.Scer\UAS under the control of Scer\GAL4^69B in a raw⁺ background.

(Byars et al., 1999)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

5789

w^*; P{UAS-aop.ACT}IIa

Kyoto

108425

w^*; P{UAS-aop.ACT}IIa/CyO

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (5)

Reported As

Symbol Synonym

Yan^CA

(Feng et al., 2017)

aop^ACT.Scer\UAS

aop^ACT.UAS

aop^CA

(Sun et al., 2020)

Name Synonyms

activated

(Rebay and Rubin, 1995)

Secondary FlyBase IDs

References (49)

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