FlyBase Allele Report: Dmel\shg[UAS.cOa]

General Information

Symbol

Dmel\shg^UAS.cOa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Oda

FlyBase ID

FBal0103569

Feature type

allele

Associated gene

Dmel\shg

Associated Insertion(s)

Carried in Construct

P{UAS-shg.DECH}

Also Known As

UAS-DECH

Key Links

Transgenic product class

cDNA

(Oda and Tsukita, 1999)

wild_type

(Oda and Tsukita, 1999)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Oda and Tsukita, 1999)

wild_type

(Oda and Tsukita, 1999)

Mutagen

in vitro construct

(Oda and Tsukita, 1999)

Progenitor genotype

Carried in construct

P{UAS-shg.DECH}

Cytology

Description

A full length shg cDNA is driven by UASt sequences.

(Oda and Tsukita, 1999)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

shg

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates cancer

modeled by Ras85D^V12.UAS, scrib¹

(Pagliarini and Xu, 2003)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

visible, with Scer\GAL4^vg.PM

(Kataoka et al., 2001)

Phenotype Manifest In

wing margin, with Scer\GAL4^vg.PM

(Kataoka et al., 2001)

Detailed Description

Statement

Reference

When transiently transformed with Scer\GAL4^Act5C.PHb into S2 cells, promoted a strong cell aggregation phenotype.

(Oda and Tsukita, 1999)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Scer\GAL4^vg.PM, shg^UAS.cOa has visible phenotype, enhanceable by AGO1^ΔN.UAS, Scer\GAL4^vg.PM

(Kataoka et al., 2001)

Suppressed by

Statement

Reference

Scer\GAL4^vg.PM, shg^UAS.cOa has visible phenotype, suppressible by AGO1^UAS.cKa, Scer\GAL4^vg.PM

(Kataoka et al., 2001)

Scer\GAL4^vg.PM, shg^UAS.cOa has visible phenotype, suppressible | partially by AGO1^ΔC.UAS, Scer\GAL4^vg.PM

(Kataoka et al., 2001)

Scer\GAL4^vg.PM, shg^UAS.cOa has visible phenotype, suppressible by Scer\GAL4^vg.PM/arm^{S2.UAS.Tag:MYC}

(Kataoka et al., 2001)

Suppressor of

Statement

Reference

shg^UAS.cOa, Scer\GAL4^Act5C.PI is a suppressor of neoplasia | somatic clone phenotype of Ras85D^V12.UAS, Scer\GAL4^Act5C.PI, scrib¹

(Pagliarini and Xu, 2003)

Phenotype Manifest In

Enhanced by

Statement

Reference

Scer\GAL4^vg.PM, shg^UAS.cOa has wing margin phenotype, enhanceable by AGO1^ΔN.UAS, Scer\GAL4^vg.PM

(Kataoka et al., 2001)

Suppressed by

Statement

Reference

Scer\GAL4^vg.PM, shg^UAS.cOa has wing margin phenotype, suppressible by AGO1^UAS.cKa, Scer\GAL4^vg.PM

(Kataoka et al., 2001)

Scer\GAL4^vg.PM, shg^UAS.cOa has wing margin phenotype, suppressible by Scer\GAL4^vg.PM/arm^{S2.UAS.Tag:MYC}

(Kataoka et al., 2001)

Scer\GAL4^vg.PM, shg^UAS.cOa has wing margin phenotype, suppressible | partially by AGO1^ΔC.UAS, Scer\GAL4^vg.PM

(Kataoka et al., 2001)

Suppressor of

Statement

Reference

shg^UAS.cOa/Scer\GAL4^slbo.2.6 is a suppressor | partially of border follicle cell phenotype of Scer\GAL4^slbo.2.6, aop^ACT.UAS

(Schober et al., 2005)

Additional Comments

Genetic Interactions

Statement

Reference

The metastatic behaviour seen in clones in the eye disc which are homozygous for scrib¹ and are also expressing Ras85D^V12.Scer\UAS under the control of Scer\GAL4^Act5C.PI is suppressed if they are co-expressing shg^Scer\UAS.cOa.

(Pagliarini and Xu, 2003)

If shg^Scer\UAS.cOa expression, in a shg²/Df(2R)E2, α-Cat^{Scer\UAS.T:Avic\GFP-sg}, Scer\GAL4^btl.PS background, is particularly high, a tracheal phenotype can be seen - dorsal trunk branches are reduced, some branches failed in fusion.

(Oda and Tsukita, 1999)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

Scer\GAL4^btl.PS/shg^UAS.cOa rescues Df(2R)E2/shg²

(Oda and Tsukita, 1999)

Comments

The addition of shg^Scer\UAS.cOa to shg², Df(2R)E2, α-Cat^{Scer\UAS.T:Avic\GFP-sg}, Scer\GAL4^btl.PS flies rescues their tracheal phenotypes to nearly wild-type. Tracheal patterning is normal, although tracheal branches and connectives in the embryos are less extend than in wild-type. The addition of shg^Scer\UAS.cOa, driven by Scer\GAL4^btl.PS, into shg²/Df(2R)E2 flies rescues their tracheal fusion phenotype.

(Oda and Tsukita, 1999)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Comments

Carried in a plasmid and co transfected with Scer\GAL4^Act5C.PHb into S2 cells.

(Oda and Tsukita, 1999)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

shg^DECH

(Taniguchi et al., 2011)

shg^Scer\UAS.cOa

shg^UAS.cOa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Oda

(Oda and Tsukita, 1999)

Secondary FlyBase IDs

References (9)

Report Sections

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