FB2024_03 , released June 25, 2024
Allele: Dmel\UbxUAS.cCa
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General Information
Symbol
Dmel\UbxUAS.cCa
Species
D. melanogaster
Name
Saccharomyces cerevisiae UAS construct a of Castelli-Gair
FlyBase ID
FBal0039101
Feature type
allele
Associated gene
Dmel\Ubx
Associated Insertion(s)
Carried in Construct
P{UAS-Ubx.Ia.C}
Also Known As
UAS-Ubx, UAS-UbxIa, UAS-Ubx Ia, UAS-UbxIa.C, UAS-Ubx.Ia.C
Key Links
Transgenic product class
cDNA
(Castelli-Gair et al., 1994)
wild_type
(Castelli-Gair et al., 1994)
Mutagen
Nature of the Allele
Transgenic product class
cDNA
(Castelli-Gair et al., 1994)
wild_type
(Castelli-Gair et al., 1994)
Mutagen
in vitro construct
(Prokop et al., 1998, Castelli-Gair et al., 1994)
Progenitor genotype
Carried in construct
P{UAS-Ubx.Ia.C}
Cytology
Description

UAS regulatory sequences drive expression of a cDNA that encodes the Ubx 1a variant plus 229bp of 5'UTR sequence and 127bp of 3'UTR sequence..

(Castelli-Gair et al., 1994)
Allele components
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
Ubx
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In

      abdominal 1 ventral denticle belt | ectopic, with Scer\GAL4arm.PS

      (Grenier and Carroll, 2000)

      abdominal 11 anal tuft, with Scer\GAL469B

      (Castelli-Gair et al., 1994)

      abdominal segment 1 & puparium & myoblast, with Scer\GAL4how-24B

      (Monier et al., 2005)

      abdominal segment 1 & puparium & myoblast, with Scer\GAL4NP5169

      (Monier et al., 2005)

      abdominal segment 1 & puparium & myofibril, with Scer\GAL4how-24B

      (Monier et al., 2005)

      abdominal segment 1 & puparium & myofibril, with Scer\GAL4NP5169

      (Monier et al., 2005)

      abdominal segment 1 | ectopic, with Scer\GAL4arm.PS

      (Li and McGinnis, 1999)

      abdominal segment 2 & puparium & myoblast, with Scer\GAL4how-24B

      (Monier et al., 2005)

      abdominal segment 2 & puparium & myoblast, with Scer\GAL4NP5169

      (Monier et al., 2005)

      abdominal segment 2 & puparium & myofibril, with Scer\GAL4how-24B

      (Monier et al., 2005)

      abdominal segment 2 & puparium & myofibril, with Scer\GAL4NP5169

      (Monier et al., 2005)

      abdominal segment 3 & puparium & myoblast, with Scer\GAL4how-24B

      (Monier et al., 2005)

      abdominal segment 3 & puparium & myoblast, with Scer\GAL4NP5169

      (Monier et al., 2005)

      abdominal segment 3 & puparium & myofibril, with Scer\GAL4how-24B

      (Monier et al., 2005)

      abdominal segment 3 & puparium & myofibril, with Scer\GAL4NP5169

      (Monier et al., 2005)

      abdominal segment 4 & puparium & myoblast, with Scer\GAL4how-24B

      (Monier et al., 2005)

      abdominal segment 4 & puparium & myoblast, with Scer\GAL4NP5169

      (Monier et al., 2005)

      abdominal segment 4 & puparium & myofibril, with Scer\GAL4how-24B

      (Monier et al., 2005)

      abdominal segment 4 & puparium & myofibril, with Scer\GAL4NP5169

      (Monier et al., 2005)

      abdominal segment 5 & puparium & myoblast | ectopic, with Scer\GAL4how-24B

      (Monier et al., 2005)

      abdominal segment 5 & puparium & myoblast | ectopic, with Scer\GAL4NP5169

      (Monier et al., 2005)

      adult head, with Scer\GAL4ey.PH

      (Jiao et al., 2001)

      antenna, with Scer\GAL4dpp.blk1

      (Grenier and Carroll, 2000, Yao et al., 1999)

      aorta primordium | anterior, with Scer\GAL4how-24B, Scer\GAL4twi.PG

      (Perrin et al., 2004)

      aorta primordium | ectopic | posterior, with Scer\GAL4how-24B, Scer\GAL4twi.PG

      (Perrin et al., 2004)

      arista, with Scer\GAL4dpp.blk1

      (Percival-Smith et al., 2005)

      cardioblast, with Scer\GAL4twi.PG

      (Ponzielli et al., 2002)

      denticle belt & abdominal segment 9, with Scer\GAL469B

      (Castelli-Gair et al., 1994)

      embryonic/larval central nervous system, with Scer\GAL4insc-Mz1407

      (Prokop et al., 1998)

      embryonic/larval dorsal vessel, with Scer\GAL4how-24B

      (Ryan et al., 2005)

      embryonic/larval lymph gland, with Scer\GAL4how-24B, Scer\GAL4twi.PG

      (Perrin et al., 2004)

      embryonic/larval ostium, with Scer\GAL4twi.PG

      (Ponzielli et al., 2002)

      embryonic/larval ostium | ectopic, with Scer\GAL4how-24B

      (Lovato et al., 2002)

      embryonic/larval pericardial cell | ectopic, with Scer\GAL4how-24B, Scer\GAL4twi.PG

      (Perrin et al., 2004)

      embryonic/larval posterior spiracle, with Scer\GAL469B

      (Castelli-Gair et al., 1994)

      embryonic/larval posterior spiracle, with Scer\GAL4h-1J3

      (Castelli-Gair et al., 1994)

      embryonic abdominal segment 1 | ectopic, with Scer\GAL4arm.PS

      (Galant and Carroll, 2002, Galant et al., 2002)

      embryonic epidermis, with Scer\GAL4arm.PS

      (Grenier and Carroll, 2000)

      embryonic head, with Scer\GAL4arm.PS

      (Li and McGinnis, 1999)

      embryonic mesothoracic segment, with Scer\GAL4arm.PS

      (Li and McGinnis, 1999)

      embryonic metathoracic segment, with Scer\GAL4arm.PS

      (Galant and Carroll, 2002, Galant et al., 2002, Li and McGinnis, 1999)

      embryonic pericardial cell, with Scer\GAL4Mef2.PR

      (Mandal et al., 2004)

      embryonic prothoracic segment, with Scer\GAL4arm.PS

      (Li and McGinnis, 1999)

      embryonic tritocerebrum, with Scer\GAL4sca-537.4

      (Sprecher et al., 2004)

      embryonic visceral muscle cell | increased number, with Scer\GAL4how-24B

      (LaBeau et al., 2009)

      eye, with Scer\GAL4ey.PH

      (Jiao et al., 2001)

      eye disc | posterior | somatic clone, with Scer\GAL4αTub84B.PL

      (Janody et al., 2004)

      gonadal sheath proper primordium | ectopic, with Scer\GAL4twi.PG

      (Greig and Akam, 1995)

      haltere | ectopic, with Scer\GAL4Bx-MS1096

      (de Navas et al., 2011, Grenier and Carroll, 2000)

      haltere | ectopic, with Scer\GAL4sd.PU

      (de Navas et al., 2011)

      interneuron | pupal stage, with Scer\GAL4GMR24B02

      (Mellert et al., 2016)

      Keilin organ, with Scer\GAL4arm.PS

      (Grenier and Carroll, 2000)

      Keilin organ, with Scer\GAL4h-1J3

      (Castelli-Gair et al., 1994)

      Keilin organ, with Scer\GAL4Kr.PM

      (Castelli-Gair et al., 1994)

      Kolbchen, with Scer\GAL4arm.PS

      (Grenier and Carroll, 2000)

      larval neuroblast | embryonic stage, with Scer\GAL4pros.PMG

      (Monedero Cobeta et al., 2017)

      larval ventral nerve cord | pupal stage, with Scer\GAL4GMR24B02

      (Mellert et al., 2016)

      leg | ectopic, with Scer\GAL4dpp.blk1

      (Grenier and Carroll, 2000)

      lymph gland primordium, with Scer\GAL4Mef2.PR

      (Mandal et al., 2004)

      mesothoracic ventral denticle belt, with Scer\GAL4arm.PS

      (Li and McGinnis, 1999)

      metathoracic ventral denticle belt, with Scer\GAL4arm.PS

      (Li and McGinnis, 1999)

      neuromere, with Scer\GAL4insc-Mz1407

      (Prokop et al., 1998)

      peripodial epithelium & dorsal mesothoracic disc, with Scer\GAL4426

      (Pallavi and Shashidhara, 2003)

      peripodial epithelium & dorsal mesothoracic disc, with Scer\GAL4vg.PM

      (Pallavi and Shashidhara, 2003)

      photoreceptor neuron | somatic clone, with Scer\GAL4αTub84B.PL

      (Janody et al., 2004)

      pretarsus | ectopic, with Scer\GAL4dpp.blk1

      (Percival-Smith et al., 2005)

      prothoracic ventral denticle belt, with Scer\GAL4arm.PS

      (Li and McGinnis, 1999)

      T1 beard | embryonic stage, with Scer\GAL4arm.PS

      (Li and McGinnis, 1999)

      thoracic neuroblast | larval stage, with Scer\GAL4αTub84B.PL

      (Bello et al., 2003)

      ventral nerve cord primordium | embryonic stage, with Scer\GAL4pros.PMG

      (Monedero Cobeta et al., 2017)

      wing, with Scer\GAL4Bx-MS1096

      (de Navas et al., 2011, Grenier and Carroll, 2000)

      wing, with Scer\GAL4dpp.blk1

      (de Navas et al., 2006)

      wing, with Scer\GAL4rn.PU

      (Percival-Smith et al., 2013)

      wing, with Scer\GAL4sd.PU

      (de Navas et al., 2011)

      wing blade, with Scer\GAL4vg.PM

      (Bischoff et al., 2009)

      wing margin, with Scer\GAL4sd-SG29.1

      (Adachi-Yamada et al., 2005)

      wing vein, with Scer\GAL4sd-SG29.1

      (Adachi-Yamada et al., 2005)
      Detailed Description
      Statement
      Reference

      The expression of UbxUAS.cCa under the control of Scer\GAL4pros.PMG induces a significant decrease in the mitotic index of neuroblast daughter cells in the embryonic ventral nerve cord T2 and T3 segments, but not in segment A1, despite of no significant changes in the mitotic index of neuroblasts in the same segments, as compared to controls.

      (Monedero Cobeta et al., 2017)

      Expression of UbxScer\UAS.cCa under the control of Scer\GAL4GMR24B02 (and thus transforming the 12A ventral nerve cord interneurons in segment T1 toward the T2 fate) leads to variable splitting and branching in T1 12A neurons at rates consistent with those typically seen in the T2 neuromere. However, within the animals ectopically expressing UbxScer\UAS.cCa in all 12A hemilineages, the proportions of neuronal wiring variants (neurite bundle split/unsplit, ectopic branches) differs between T1 and T2 segment neurons in the pupal brain.

      (Mellert et al., 2016)

      Expression of UbxScer\UAS.cCa under the control of Scer\GAL4rn.PU suppresses wing formation.

      (Percival-Smith et al., 2013)

      Expression of UbxScer\UAS.cCa under the control of Scer\GAL4sd.PU or Scer\GAL4Bx-MS1096 results in a transformation of wing to haltere.

      (de Navas et al., 2011)

      Ectopic expression of UbxScer\UAS.cCa driven by Scer\GAL4vg.PM leads to small, crumpled wings with small necrotic cells.

      (Bischoff et al., 2009)

      Embryos ectopically expressing Scer\GAL4how-24B driven UbxScer\UAS.cCa throughout the mesoderm show an increased number of alary muscle pairs, from the seven normally observed in wild-type, to approximately ten. The supernumerary muscles are usually paired, and in many cases the most anterior pair of muscles is angled acutely to ensure contact with the dorsal vessel.

      (LaBeau et al., 2009)

      Expression of UbxScer\UAS.cCa under the control of Scer\GAL4dpp.blk1 results in flies with smaller wings than normal.

      (de Navas et al., 2006)

      When UbxScer\UAS.cCa is driven by Scer\GAL4sd-SG29.1 abnormal wing vein patterns and incisions in the wing margin are seen.

      (Adachi-Yamada et al., 2005)

      UbxScer\UAS.cCa expression in all muscle cells at the onset of metamorphosis under the control of Scer\GAL4how-24B (and Scer\GAL80ts.αTub84B, placed at the restrictive temperature of 29oC at the onset of metamorphosis) results in tin-expressing myocytes in segments A1-A4 adopting the characteristics of adult segment A5 tin-expressing myocytes, including longitudinal orientation of the muscle fibers. UbxScer\UAS.cCa expression in cardiac myocytes under the control of Scer\GAL4NP5169 (and Scer\GAL80ts.αTub84B, blocking Scer\GAL4NP5169, placed at the restrictive temperature of 29oC at the onset of metamorphosis) results in tin-expressing myocytes in segments A1-A4 adopting the characteristics of adult segment A5 tin-expressing myocytes, including longitudinal orientation of the muscle fibers. UbxScer\UAS.cCa expression in all muscle cells at the onset of metamorphosis under the control of Scer\GAL4how-24B (and Scer\GAL80ts.αTub84B, placed at the restrictive temperature of 29oC at the onset of metamorphosis) does not affect the differentiation of adult ostiae that develop from A1-A5 svp-expressing cells.

      (Monier et al., 2005)

      Expression of UbxScer\UAS.cCa under the control of Scer\GAL4dpp.blk1 results in transformation of the arista to tarsus.

      (Percival-Smith et al., 2005)

      In stage 16 UbxScer\UAS.cCa; Scer\GAL4how-24B embryos, the number of cells in the dorsal vessel is increased from 104 to 116 cells compared to wild-type.

      (Ryan et al., 2005)

      When clones expressing UbxScer\UAS.cCa (driven by Scer\GAL4αTub84B.PL) in the posterior of eye disc, photoreceptor differentiation is blocked .

      (Janody et al., 2004)

      In stage-15 mutant embryos in which UbxScer\UAS.cCa is overexpressed under the control of Scer\GAL4Mef2.PR, lymph-gland progenitors are replaced by pericardial nephrocytes.

      (Mandal et al., 2004)

      In mutant embryos expressing UbxScer\UAS.cCa driven by both Scer\GAL4twi.PG and Scer\GAL4how-24B ectopic cardioblasts are seen and the lymph glands are systematically eliminated and replaced by major pericardial cells. The anterior aorta is also transformed into a posterior aorta and heart tissue.

      (Perrin et al., 2004)

      Expression of UbxScer\UAS.cCa under the control of Scer\GAL4sca-537.4 results in a mutant phenotype in the embryonic tritocerebrum. The phenotype has a penetrance of more than 95%.

      (Sprecher et al., 2004)

      Postembryonic neuroblast (pNB) clones in the larval thorax expressing UbxScer\UAS.cCa under the control of Scer\GAL4αTub84B.PL are reduced in size compared to wild-type clones and the clones show loss of the pNB in at least 82% of cases.

      (Bello et al., 2003)

      The number of peripodial membrane cells in the wing discs of UbxScer\UAS.cCa; Scer\GAL4vg.PM late 3rd instar larvae is decreased to less than 250 (from 400-450 in wild-type, wheras with UbxScer\UAS.cCa; Scer\GAL4426 it is increased to >550.

      (Pallavi and Shashidhara, 2003)

      Expression of UbxScer\UAS.cCa under the control of Scer\GAL4en-e16E does not result in the formation of ectopic oenocytes in the T1-T3 segments.

      (Brodu et al., 2002)

      Expression of UbxScer\UAS.cCa under the control of Scer\GAL4arm.PS in embryos results in the transformation of segment T3 into A1. The penetrance of this phenotype is 100%.

      (Galant and Carroll, 2002)

      Expression of UbxScer\UAS.cCa under the control of Scer\GAL4arm.PS in embryos results in the transformation of the identity of third thoracic segment to that of abdominal segment A1.

      (Galant et al., 2002)

      Expression of UbxScer\UAS.cCa under the control of Scer\GAL4how-24B in embryos does not inhibit heart cell specification in segments A5-A8. There is often an increase in cell size in the dorsal vessel at the A4/A5 boundary, consistent with the formation of an ectopic ostium within the aorta.

      (Lovato et al., 2002)

      Cardioblasts do not have normal morphology in embryos expressing UbxScer\UAS.cCa under the control of both Scer\GAL4twi.PG and Scer\GAL4how-24B and the ostiae are not functional.

      (Ponzielli et al., 2002)

      94% of flies expressing UbxScer\UAS.cCa under the control of Scer\GAL4ey.PH are wild type. Some animals are seen in which most of the head and one or both eyes of reduced size are present.

      (Jiao et al., 2001)

      Overexpression of UbxScer\UAS.cCa driven by Scer\GAL4dpp.blk1 causes transformation of the antenna towards a thoracic leg. Overexpression of UbxScer\UAS.cCa driven by Scer\GAL4Bx-MS1096 causes transformation of the wing towards a haltere in females. Males do not survive larval stages. The phenotype is stronger on the dorsal surface of the wing. Overexpression of UbxScer\UAS.cCa in the ectoderm, driven by Scer\GAL4arm.PS, transforms the denticle belts of thoracic segments towards A1 identity. The development of Keilin's organs and ventral pits are repressed.

      (Grenier and Carroll, 2000)

      When ectopically expressed by a strong Scer\GAL4arm.PS (arm-GAL4), UbxScer\UAS.cCa transforms head and all three thoracic segments into morphological replicas of A1 i.e. generating A1-like denticle belts in these more anterior segments. T1 develops without the characteristic patch of beard denticles. When ectopically expressed by a weak Scer\GAL4arm.PS (arm-GAL4r), UbxScer\UAS.cCa shows no abnormalities at all.

      (Li and McGinnis, 1999)

      The overall shape of the central nervous system is not perturbed in embryos expressing UbxScer\UAS.cCa under the control of Scer\GAL4Mz1407. The pattern of BrdU incorporation in the central nervous system is abnormal in stage 16/17 embryos; the lateral replicating clusters of the thoracic neuromeres are abolished, and ectopic sites of BrdU incorporation are seen in the ventral region of the thoracic neuromeres.

      (Prokop et al., 1998)

      Scer\GAL4twi.PG induced expression causes ectopic gonadal mesoderm formation in anterior segments of the embryo. Df(3R)Ubx109/Df(3R)P9 embryos lack gonads, ectopic expression of Ubx restores the formation of an encapsulated gonad.

      (Greig and Akam, 1995)

      Keilin's organs are suppressed in the UbxScer\UAS.cCa, Scer\GAL4h-1J3 or UbxScer\UAS.cCa, Scer\GAL4Kr.PM combination. With Scer\GAL469B, posterior spiracles are reduced to half their wild type size, in most embryos a small A9 denticle belt is formed anterior to the anal plates and the anal tuft is often reduced. Similar but weaker effects on the posterior spiracles are seen with Scer\GAL4h-1J3.

      (Castelli-Gair et al., 1994)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      NOT Enhanced by
      Suppressed by
      NOT Enhancer of
      Other
      Phenotype Manifest In
      NOT Enhanced by
      Suppressed by
      NOT Enhancer of
      Other
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      The decreased mitotic index of neuroblast daughter cells in the embryonic ventral nerve cord T2 and T3 segments induced by the expression of UbxUAS.cCa under the control of Scer\GAL4pros.PMG is not enhanced by Df(3L)ED225 or by the additional double co-expression of abd-AUAS.Tag:FLAG and Abd-BUAS.Tag:V5.

      Df(3L)ED225 mutants also expressing UbxUAS.cCa under the control of Scer\GAL4pros.PMG show a significant decrease in the mitotic index of neuroblasts in the embryonic ventral nerve cord T2 and T3 segments, as compared to either single background condition.

      The triple co-expression of UbxUAS.cCa, abd-AUAS.Tag:FLAG and Abd-BUAS.Tag:V5 leads to a significant decrease in the number of NB3-5T lineage cells (Scer\GAL4GMR80G10-driving expression) and NB5-6T lineage cells (Scer\GAL4pros.PMG-driving expression) in the T1 and T3 segments, associated with both a severe decrease in the mitotic index of neuroblasts in the NB5-6T lineage, but not in the NB3-5T lineage, and a severe decrease in the mitotic index of the neuroblast daughter cells in the NB3-5T lineage, but not in the NB5-6T lineage, as compared to wild-type controls.

      (Monedero Cobeta et al., 2017)

      Addition of UbxScer\UAS.cCa to Dfd16;Scr4 double mutant embryos (under the control of Scer\GAL4salm-459.2) results in both the Mx and Lb segments forming tracheal tubes instead of migratory gland primordia.

      (Sánchez-Higueras et al., 2014)

      Co-expression of pbScer\UAS.T:Avic\GFP-EGFP suppresses the arista-to-tarsus transformation seen in flies expressing UbxScer\UAS.cCa under the control of Scer\GAL4dpp.blk1.

      (Percival-Smith et al., 2005)

      The defect in the embryonic tritocerebrum caused by expression of UbxScer\UAS.cCa under the control of Scer\GAL4sca-537.4 is completely suppressed if the embryos are also coexpressing both exdScer\UAS.NLS.T:Zzzz\FLAG and hthScer\UAS.cPa.

      (Sprecher et al., 2004)

      Co-expression of hthScer\UAS.cPa partially suppresses the antenna to leg transformation phenotypes produced by ectopic expression of UbxScer\UAS.cCa driven by Scer\GAL4dpp.blk1. The antennae produced are aristaless and occasionally duplicated, similar to the hthScer\UAS.cPa phenotype.

      (Yao et al., 1999)

      The segmentation phenotype of Df(3R)Ubx109 is modified in the UbxScer\UAS.cCa, Scer\GAL4h-1J3 combination to transform alternate segments to resemble a wild type A1. This transformation is complete in the UbxScer\UAS.cCa, Scer\GAL469B combination. The sensory organ phenotype of Df(3R)Ubx109 is modified in the UbxScer\UAS.cCa, Scer\GAL4h-1J3 or UbxScer\UAS.cCa, Scer\GAL4Kr.PM combination to prevent the ventral migration of the cells that would form the kolbchen, though later differentiation produces misplaced kolbchen rather than dorsal hairs. The sensory organ phenotype of Df(3R)Ubx109 is modified in the UbxScer\UAS.cCa, Scer\GAL469B combination to affect the migration and differentiation of the kolbchen.

      (Castelli-Gair et al., 1994)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments

      The partial transformation of haltere into wing which is seen in UbxLDN/Ubxabx-1 Ubxbx-3 Ubxpbx-1 flies is strongly suppressed by expression of UbxScer\UAS.cCa under the control of Scer\GAL4Ubx-LDN.

      (de Navas et al., 2011)

      Expression of UbxScer\UAS.cCa rescues the phenotype of UbxLDN, Ubxbx-3, Ubxpbx-1, Ubxabx-1 flies.

      (de Navas et al., 2006)
      Images (0)
      Mutant
      Wild-type
      Stocks (2)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      UbxScer\UAS.cCa
      UbxUAS.cCa
      Name Synonyms
      Saccharomyces cerevisiae UAS construct a of Castelli-Gair
      (Castelli-Gair et al., 1994)
      Secondary FlyBase IDs
        References (58)