FB2024_03 , released June 25, 2024
Allele: Dmel\Abd-BUAS.Tag:V5
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General Information
Symbol
Dmel\Abd-BUAS.Tag:V5
Species
D. melanogaster
Name
FlyBase ID
FBal0340257
Feature type
allele
Associated gene
Dmel\Abd-B
Associated Insertion(s)
Carried in Construct
PBac{UAS-Abd-B.V5}
Also Known As
UAS-Abd-B
Key Links
Transgenic product class
wild_type
(Monedero Cobeta et al., 2017)
Mutagen
Nature of the Allele
Transgenic product class
wild_type
(Monedero Cobeta et al., 2017)
Mutagen
in vitro construct
(Monedero Cobeta et al., 2017)
Progenitor genotype
Carried in construct
PBac{UAS-Abd-B.V5}
Cytology
Description

UASt regulatory sequences drive expression of Abd-B (codon-optimized for expression in Drosophila), tagged with Tag:V5.

(Monedero Cobeta et al., 2017)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
Abd-B
Tagged with
Tag:V5
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      The expression of Abd-BUAS.Tag:V5 under the control of Scer\GAL4pros.PMG induces a significant decrease in the mitotic index of neuroblast daughter cells, but not of neuroblasts, in the embryonic ventral nerve cord T2 and T3 segments, as compared to controls.

      (Monedero Cobeta et al., 2017)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      NOT Enhanced by
      NOT Enhancer of
      Other
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      The decreased mitotic index of neuroblast daughter cells in the embryonic ventral nerve cord T2 and T3 segments induced by the expression of Abd-BUAS.Tag:V5 under the control of Scer\GAL4pros.PMG is not enhanced by Df(3L)ED225 or by the additional co-expression of UbxUAS.cCa and abd-AUAS.Tag:FLAG.

      Df(3L)ED225 mutants also expressing Abd-BUAS.Tag:V5 under the control of Scer\GAL4pros.PMG do not show a significant change in the mitotic index of neuroblasts in the embryonic ventral nerve cord T2 and T3 segments, as compared to wild-type controls.

      The triple co-expression of UbxUAS.cCa, abd-AUAS.Tag:FLAG and Abd-BUAS.Tag:V5 leads to a significant decrease in the number of NB3-5T lineage cells (Scer\GAL4GMR80G10-driving expression) and NB5-6T lineage cells (Scer\GAL4pros.PMG-driving expression) in the T1 and T3 segments, associated with both a severe decrease in the mitotic index of neuroblasts in the NB5-6T lineage, but not in the NB3-5T lineage, and a severe decrease in the mitotic index of the neuroblast daughter cells in the NB3-5T lineage, but not in the NB5-6T lineage, as compared to wild-type controls.

      (Monedero Cobeta et al., 2017)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (1)
      Reported As
      Symbol Synonym
      Abd-BUAS.Tag:V5
      Name Synonyms
      Secondary FlyBase IDs
        References (4)