FlyBase Allele Report: Dmel\pb[UAS.EGFP]

General Information

Symbol

Dmel\pb^UAS.EGFP

Species

D. melanogaster

Name

FlyBase ID

FBal0280820

Feature type

allele

Associated gene

Dmel\pb

Associated Insertion(s)

Carried in Construct

P{UAS-pb.GFP}

Key Links

Transgenic product class

wild_type

(Percival-Smith et al., 2005)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Percival-Smith et al., 2005)

Mutagen

in vitro construct

(Percival-Smith et al., 2005)

Progenitor genotype

Carried in construct

P{UAS-pb.GFP}

Cytology

Description

UASt regulatory sequences drive expression of full-length pb which is fused (via a 5 amino acid LSWSS linker) at the C-terminal end to EGFP.

(Percival-Smith et al., 2005)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Tagged with

EGFP

green fluorescent protein

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

arista, with Scer\GAL4^dpp.blk1

(Percival-Smith et al., 2005)

maxillary palp | ectopic, with Scer\GAL4^pb.PJ

(Percival-Smith et al., 2013)

Detailed Description

Statement

Reference

Expression of pb^{Scer\UAS.T:Avic\GFP-EGFP} under the control of Scer\GAL4^pb.PJ results in an antenna to maxillary palp transformation.

(Percival-Smith et al., 2013)

Expression of pb^{Scer\UAS.T:Avic\GFP-EGFP} under the control of Scer\GAL4^dpp.blk1 does not result in an arista-to-tarsus transformation, but deletion of distal segments is seen in 55% of cases.

(Percival-Smith et al., 2005)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

pb^UAS.EGFP, Scer\GAL4^dpp.blk1 is a suppressor of visible phenotype of Dfd^UAS.cLa, Scer\GAL4^dpp.blk1

(Percival-Smith et al., 2005)

pb^UAS.EGFP, Scer\GAL4^dpp.blk1 is a suppressor of visible phenotype of Antp^UAS.cBa, Scer\GAL4^dpp.blk1

(Percival-Smith et al., 2005)

pb^UAS.EGFP/Scer\GAL4^dpp.blk1 is a suppressor of visible phenotype of Scer\GAL4^dpp.blk1, Ubx^UAS.cCa

(Percival-Smith et al., 2005)

pb^UAS.EGFP/Scer\GAL4^dpp.blk1 is a suppressor of visible phenotype of Scer\GAL4^dpp.blk1, abd-A^UAS.cGa

(Percival-Smith et al., 2005)

pb^UAS.EGFP, Scer\GAL4^dpp.blk1 is a suppressor of visible phenotype of Abd-B^UAS.cCa, Scer\GAL4^dpp.blk1

(Percival-Smith et al., 2005)

pb^UAS.EGFP/Scer\GAL4^dpp.blk1 is a suppressor of visible phenotype of Scer\GAL4^dpp.blk1, Scr^UAS.cMa

(Percival-Smith et al., 2005)

Phenotype Manifest In

NOT suppressed by

Statement

Reference

Scer\GAL4^pb.PJ, pb^UAS.EGFP has maxillary palp | ectopic phenotype, non-suppressible by Scr^UAS.cPb/Scer\GAL4^pb.PJ

(Percival-Smith et al., 2013)

Scer\GAL4^pb.PJ, pb^UAS.EGFP has maxillary palp | ectopic phenotype, non-suppressible by Scer\GAL4^pb.PJ/Scr^UAS.Tag:FLAG

(Percival-Smith et al., 2013)

Scer\GAL4^pb.PJ, pb^UAS.EGFP has maxillary palp | ectopic phenotype, non-suppressible by Scr^Δocta.UAS/Scer\GAL4^pb.PJ

(Percival-Smith et al., 2013)

Scer\GAL4^pb.PJ, pb^UAS.EGFP has maxillary palp | ectopic phenotype, non-suppressible by Scr^{Δocta.CTD.UAS}/Scer\GAL4^pb.PJ

(Percival-Smith et al., 2013)

Scer\GAL4^pb.PJ, pb^UAS.EGFP has maxillary palp | ectopic phenotype, non-suppressible by Scr^{ΔKMAS.CTD.UAS}/Scer\GAL4^pb.PJ

(Percival-Smith et al., 2013)

Scer\GAL4^pb.PJ, pb^UAS.EGFP has maxillary palp | ectopic phenotype, non-suppressible by Scr^{ΔDYTQL.TS.CTD.UAS}/Scer\GAL4^pb.PJ

(Percival-Smith et al., 2013)

Suppressor of

Statement

Reference

pb^UAS.EGFP, Scer\GAL4^dpp.blk1 is a suppressor of pretarsus | ectopic phenotype of Dfd^UAS.cLa, Scer\GAL4^dpp.blk1

(Percival-Smith et al., 2005)

pb^UAS.EGFP, Scer\GAL4^dpp.blk1 is a suppressor of arista phenotype of Antp^UAS.cBa, Scer\GAL4^dpp.blk1

(Percival-Smith et al., 2005)

pb^UAS.EGFP, Scer\GAL4^dpp.blk1 is a suppressor of pretarsus | ectopic phenotype of Antp^UAS.cBa, Scer\GAL4^dpp.blk1

(Percival-Smith et al., 2005)

pb^UAS.EGFP/Scer\GAL4^dpp.blk1 is a suppressor of arista phenotype of Scer\GAL4^dpp.blk1, Ubx^UAS.cCa

(Percival-Smith et al., 2005)

pb^UAS.EGFP/Scer\GAL4^dpp.blk1 is a suppressor of pretarsus | ectopic phenotype of Scer\GAL4^dpp.blk1, Ubx^UAS.cCa

(Percival-Smith et al., 2005)

pb^UAS.EGFP/Scer\GAL4^dpp.blk1 is a suppressor of arista phenotype of Scer\GAL4^dpp.blk1, abd-A^UAS.cGa

(Percival-Smith et al., 2005)

pb^UAS.EGFP/Scer\GAL4^dpp.blk1 is a suppressor of pretarsus | ectopic phenotype of Scer\GAL4^dpp.blk1, abd-A^UAS.cGa

(Percival-Smith et al., 2005)

pb^UAS.EGFP, Scer\GAL4^dpp.blk1 is a suppressor of arista phenotype of Abd-B^UAS.cCa, Scer\GAL4^dpp.blk1

(Percival-Smith et al., 2005)

pb^UAS.EGFP, Scer\GAL4^dpp.blk1 is a suppressor of pretarsus | ectopic phenotype of Abd-B^UAS.cCa, Scer\GAL4^dpp.blk1

(Percival-Smith et al., 2005)

pb^UAS.EGFP/Scer\GAL4^dpp.blk1 is a suppressor of arista phenotype of Scer\GAL4^dpp.blk1, Scr^UAS.cMa

(Percival-Smith et al., 2005)

pb^UAS.EGFP/Scer\GAL4^dpp.blk1 is a suppressor of pretarsus | ectopic phenotype of Scer\GAL4^dpp.blk1, Scr^UAS.cMa

(Percival-Smith et al., 2005)

pb^UAS.EGFP, Scer\GAL4^dpp.blk1 is a suppressor of arista phenotype of Dfd^UAS.cLa, Scer\GAL4^dpp.blk1

(Percival-Smith et al., 2005)

Other

Statement

Reference

Scer\GAL4^pb.PJ, Scr^UAS.BFP, pb^UAS.EGFP has proboscis | ectopic phenotype

(Percival-Smith et al., 2013)

Scer\GAL4^pb.PJ, Scr^ΔLASCY.UAS, pb^UAS.EGFP has proboscis | ectopic phenotype

(Percival-Smith et al., 2013)

Scer\GAL4^pb.PJ, Scr^{ΔLASCY.NANGE.CTD.UAS}, pb^UAS.EGFP has proboscis | ectopic phenotype

(Percival-Smith et al., 2013)

Scer\GAL4^pb.PJ, Scr^{ΔDYTQL.KMAS.CTD.UAS}, pb^UAS.EGFP has proboscis | ectopic phenotype

(Percival-Smith et al., 2013)

Scer\GAL4^pb.PJ, Scr^{Δocta.LASCY.DYTQL.CTD.UAS}, pb^UAS.EGFP has proboscis | ectopic phenotype

(Percival-Smith et al., 2013)

Scer\GAL4^pb.PJ, Scr^{ΔLASCY.DYTQL.TS.CTD.UAS}, pb^UAS.EGFP has proboscis | ectopic phenotype

(Percival-Smith et al., 2013)

Scer\GAL4^pb.PJ, Scr^{ΔDYTQL.NANGE.TS.CTD.UAS}, pb^UAS.EGFP has proboscis | ectopic phenotype

(Percival-Smith et al., 2013)

Scer\GAL4^pb.PJ, Scr^{ΔLASCY.DYTQL.NANGE.TS.CTD.UAS}, pb^UAS.EGFP has proboscis | ectopic phenotype

(Percival-Smith et al., 2013)

Scer\GAL4^pb.PJ, Scr::Mmus\Hoxa5^{Scr-Hoxa5.UAS}, pb^UAS.EGFP has proboscis | ectopic phenotype

(Percival-Smith et al., 2013)

Scer\GAL4^pb.PJ, Scr^ΔDYTQL.UAS, pb^UAS.EGFP has proboscis | ectopic phenotype

(Percival-Smith et al., 2013)

Scer\GAL4^pb.PJ, Scr^ΔNANGE.UAS, pb^UAS.EGFP has proboscis | ectopic phenotype

(Percival-Smith et al., 2013)

Scer\GAL4^pb.PJ, Scr^ΔCTD.UAS, pb^UAS.EGFP has proboscis | ectopic phenotype

(Percival-Smith et al., 2013)

Scer\GAL4^pb.PJ, Scr^{ΔLASCY.DYTQL.UAS}, pb^UAS.EGFP has proboscis | ectopic phenotype

(Percival-Smith et al., 2013)

Scer\GAL4^pb.PJ, Scr^{ΔDYTQL.CTD.UAS}, pb^UAS.EGFP has proboscis | ectopic phenotype

(Percival-Smith et al., 2013)

Scer\GAL4^pb.PJ, Scr^{Δocta.LASCY.CTD.UAS}, pb^UAS.EGFP has proboscis | ectopic phenotype

(Percival-Smith et al., 2013)

Scer\GAL4^pb.PJ, Scr^{Δocta.DYTQL.CTD.UAS}, pb^UAS.EGFP has proboscis | ectopic phenotype

(Percival-Smith et al., 2013)

Scer\GAL4^pb.PJ, Scr^{ΔLASCY.DYTQL.CTD.UAS}, pb^UAS.EGFP has proboscis | ectopic phenotype

(Percival-Smith et al., 2013)

Additional Comments

Genetic Interactions

Statement

Reference

Co-expression of Scr^{Scer\UAS.T:Avic\GFP-EBFP} and pb^{Scer\UAS.T:Avic\GFP-EGFP} under the control of Scer\GAL4^pb.PJ results in an antenna to proboscis transformation.

Co-expression of Scr^Scer\UAS.cPb and pb^{Scer\UAS.T:Avic\GFP-EGFP} under the control of Scer\GAL4^pb.PJ does not result in an antenna to proboscis transformation. The antenna is transformed to maxillary palp, as in seen when pb^{Scer\UAS.T:Avic\GFP-EGFP} is expressed alone.

Co-expression of Scr^{Scer\UAS.T:Zzzz\FLAG} and pb^{Scer\UAS.T:Avic\GFP-EGFP} under the control of Scer\GAL4^pb.PJ does not result in transformation of the antenna to proboscis.

Co-expression of Scr^{Δocta.Scer\UAS} and pb^{Scer\UAS.T:Avic\GFP-EGFP} under the control of Scer\GAL4^pb.PJ does not result in transformation of the antenna to proboscis.

Co-expression of Scr^{ΔLASCY.Scer\UAS} and pb^{Scer\UAS.T:Avic\GFP-EGFP} under the control of Scer\GAL4^pb.PJ results in a mild transformation of the antenna to proboscis, but this is not significantly different from controls.

Co-expression of Scr^{ΔDYTQL.Scer\UAS} and pb^{Scer\UAS.T:Avic\GFP-EGFP} under the control of Scer\GAL4^pb.PJ results in a mild transformation of the antenna to proboscis, but this is not significantly different from controls.

Co-expression of Scr^{ΔNANGE.Scer\UAS} and pb^{Scer\UAS.T:Avic\GFP-EGFP} under the control of Scer\GAL4^pb.PJ results in a mild but significant transformation of the antenna to proboscis.

Co-expression of Scr^{ΔTS.Scer\UAS} and pb^{Scer\UAS.T:Avic\GFP-EGFP} under the control of Scer\GAL4^pb.PJ results in a mild transformation of the antenna to proboscis, but this is not significantly different from controls.

Co-expression of Scr^{ΔKMAS.Scer\UAS} and pb^{Scer\UAS.T:Avic\GFP-EGFP} under the control of Scer\GAL4^pb.PJ results in a mild transformation of the antenna to proboscis, but this is not significantly different from controls.

Co-expression of Scr^{ΔCTD.Scer\UAS} and pb^{Scer\UAS.T:Avic\GFP-EGFP} under the control of Scer\GAL4^pb.PJ results in a strong transformation of the antenna to proboscis.

Co-expression of Scr^{Δocta.CTD.Scer\UAS} and pb^{Scer\UAS.T:Avic\GFP-EGFP} under the control of Scer\GAL4^pb.PJ does not result in transformation of the antenna to proboscis.

Co-expression of Scr^{ΔLASCY.DYTQL.Scer\UAS} and pb^{Scer\UAS.T:Avic\GFP-EGFP} under the control of Scer\GAL4^pb.PJ results in a strong transformation of the antenna to proboscis.

Co-expression of Scr^{ΔDYTQL.CTD.Scer\UAS} and pb^{Scer\UAS.T:Avic\GFP-EGFP} under the control of Scer\GAL4^pb.PJ results in a strong transformation of the antenna to proboscis. This phenotype is seen at room temperature or 28.5[o]C. Tricombless cuticle is also seen in the maxillary palp. No evidence of a labial cap and chemosensory bristles is observed in theses patches of tricombless cuticle.

Co-expression of Scr^{ΔKMAS.CTD.Scer\UAS} and pb^{Scer\UAS.T:Avic\GFP-EGFP} under the control of Scer\GAL4^pb.PJ does not result in transformation of the antenna to proboscis.

Co-expression of Scr^{Δocta.LASCY.CTD.Scer\UAS} and pb^{Scer\UAS.T:Avic\GFP-EGFP} under the control of Scer\GAL4^pb.PJ results in a strong transformation of the antenna to proboscis in some flies.

Co-expression of Scr^{Δocta.DYTQL.CTD.Scer\UAS} and pb^{Scer\UAS.T:Avic\GFP-EGFP} under the control of Scer\GAL4^pb.PJ results in a strong transformation of the antenna to proboscis in some flies.

Co-expression of Scr^{ΔLASCY.DYTQL.CTD.Scer\UAS} and pb^{Scer\UAS.T:Avic\GFP-EGFP} under the control of Scer\GAL4^pb.PJ results in a weak transformation of the antenna to proboscis.

Co-expression of Scr^{ΔLASCY.NANGE.CTD.Scer\UAS} and pb^{Scer\UAS.T:Avic\GFP-EGFP} under the control of Scer\GAL4^pb.PJ results in a strong transformation of the antenna to proboscis.

Co-expression of Scr^{ΔDYTQL.TS.CTD.Scer\UAS} and pb^{Scer\UAS.T:Avic\GFP-EGFP} under the control of Scer\GAL4^pb.PJ does not result in transformation of the antenna to proboscis.

Co-expression of Scr^{ΔDYTQL.KMAS.CTD.Scer\UAS} and pb^{Scer\UAS.T:Avic\GFP-EGFP} under the control of Scer\GAL4^pb.PJ results in a strong transformation of the antenna to proboscis.

Co-expression of Scr^{Δocta.LASCY.DYTQL.CTD.Scer\UAS} and pb^{Scer\UAS.T:Avic\GFP-EGFP} under the control of Scer\GAL4^pb.PJ does not result in transformation of the antenna to proboscis.

Co-expression of Scr^{ΔLASCY.DYTQL.TS.CTD.Scer\UAS} and pb^{Scer\UAS.T:Avic\GFP-EGFP} under the control of Scer\GAL4^pb.PJ results in strong transformation of the antenna to proboscis in some flies.

Co-expression of Scr^{ΔDYTQL.NANGE.TS.CTD.Scer\UAS} and pb^{Scer\UAS.T:Avic\GFP-EGFP} under the control of Scer\GAL4^pb.PJ results in weak transformation of the antenna to proboscis.

Co-expression of Scr^{ΔLASCY.DYTQL.NANGE.TS.CTD.Scer\UAS} and pb^{Scer\UAS.T:Avic\GFP-EGFP} under the control of Scer\GAL4^pb.PJ results in weak transformation of the antenna to proboscis in some flies.

(Percival-Smith et al., 2013)

Co-expression of pb^{Scer\UAS.T:Avic\GFP-EGFP} suppresses the arista-to-tarsus transformation seen in flies expressing either Antp^Scer\UAS.cBa, Dfd^Scer\UAS.cLa, Scr^Scer\UAS.cMa, Ubx^Scer\UAS.cCa, abd-A^Scer\UAS.cGa or Abd-B^Scer\UAS.cCa under the control of Scer\GAL4^dpp.blk1.

(Percival-Smith et al., 2005)

Xenogenetic Interactions

Statement

Reference

Co-expression of Mmus\Hoxa5^Scer\UAS.cPa and pb^{Scer\UAS.T:Avic\GFP-EGFP} under the control of Scer\GAL4^pb.PJ does not result in transformation of the antenna to proboscis.

Co-expression of Scr::Mmus\Hoxa5^{Scr-Hoxa5.Scer\UAS} and pb^{Scer\UAS.T:Avic\GFP-EGFP} under the control of Scer\GAL4^pb.PJ results in a strong transformation of the antenna to proboscis.

(Percival-Smith et al., 2013)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

pb^{Scer\UAS.T:Avic\GFP-EGFP}

pb^UAS.EGFP

Name Synonyms

Secondary FlyBase IDs

References (2)

Report Sections

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